scholarly journals 740. Rapid Adenoviral Capsid Engineering (RACE) Platform for High-Throughput Modification of High-Capacity Adenoviral Vectors

2009 ◽  
Vol 17 ◽  
pp. S282-S283
Keyword(s):  
High Throughput ◽  
High Capacity ◽  
Adenoviral Vectors

scholarly journals Ultra-high capacity microfluidic trapping of giant vesicles for high-throughput membrane studies

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 626-633 ◽  
Author(s):  
Naresh Yandrapalli ◽  
Tom Robinson
Keyword(s):  
High Throughput ◽  
High Capacity ◽  
Giant Vesicles ◽  
Microfluidic Platform

A high-capacity microfluidic platform designed to capture tens of thousands of giant vesicles for high-throughput membrane analysis.

Production and Quality Control of High-Capacity Adenoviral Vectors

Cell Biology ◽  
2006 ◽  
pp. 445-456 ◽  
Author(s):  
G SCHIEDNER ◽  
F KREPPEL ◽  
S KOCHANEK
Keyword(s):  
Quality Control ◽  
High Capacity ◽  
Adenoviral Vectors

scholarly journals Herpes Simplex Virus Type 1 Thymidine Kinase Sequence Fused to the lacZ Gene Increases Levels of β-Galactosidase Activity per Genome of High-Capacity but Not First-Generation Adenoviral Vectors In Vitro and In Vivo

2008 ◽  
Vol 83 (4) ◽  
pp. 2004-2010 ◽  
Author(s):  
M. Puntel ◽  
R. J. Barrett ◽  
S. Mondkar ◽  
V. Saxena ◽  
K. M. Kroeger ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transgene Expression ◽  
First Generation ◽  
High Capacity ◽  
Adenoviral Vectors ◽  
Simplex Virus

ABSTRACT Increased transgene expression per vector genome is an important goal in the optimization of viral vectors for gene therapy. Herein we demonstrate that herpes simplex virus type 1 (HSV1) thymidine kinase (TK) gene sequences (1,131 bp) fused to the 3′ end of lacZ increase transgene expression from high-capacity adenoviral vectors (HCAd), but not from first-generation (Ad) vectors. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), in contrast, increased transgene expression levels from Ad but not HCAd vectors. The differential activity of the HSV1 TK gene and WPRE sequences was detected both in vitro and in vivo and suggests potentially different mechanisms of action or the interaction of these elements with vector genomic sequences.

scholarly journals Glycated Haemoglobin: An Assessment of High Capacity Liquid Chromatographic and Immunoassay Methods

1992 ◽  
Vol 29 (5) ◽  
pp. 494-505 ◽  
Author(s):  
Susan J Standing ◽  
Richard P Taylor
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Throughput ◽  
High Performance ◽  
High Capacity ◽  
Glycated Haemoglobin ◽  
Coefficients Of Variation ◽  
Operation Flexibility ◽  
Liquid Chromatographic

We have assessed five high-throughput systems for the measurement of glycated haemoglobin and have reviewed published evaluations of individual analysers. All systems offered better precision than a widely used electroendosmosis method. The low pressure chromatography and immunoassay systems demonstrated greater between-batch imprecision than the high performance liquid chromatography analysers, the latter achieving the proposed analytical goal of between-batch coefficients of variation less than 5%. Agreement between all systems measuring HbA1 was good but there was variability amongst observed HbA1c values. The systems were also assessed for their quality of chromatographic separation, simplicity of operation, flexibility, cost and potential for interference by other haemoglobins.

Stacker Modules Used in a High-Capacity Robotics System for High Throughput Screening Compound Replication

1999 ◽  
Vol 4 (6) ◽  
pp. 373-379 ◽  
Author(s):  
Mark Beggs ◽  
Herman Blok ◽  
Jan Mertens
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
High Capacity ◽  
Robotic System ◽  
Throughput Capacity ◽  
Process Automation ◽  
Technology Process ◽  
Human Operators ◽  
Automation Project ◽  
Logistics Control

High throughput screening is now established as a key component of the pharmaceutical lead identification process in many pharmaceutical companies. Over recent years, thanks to advances in assay technology, process automation, and logistics control, the throughput capacity of HTS groups has increased significantly. It is now entirely possible to screen corporate compound collections against an individual pharmacological target within a timescale of several weeks. Despite these improvements, many HTS groups find that their capacity is limited by the rate at which they can provide test compounds in a "screen-ready" format. This limitation is usually imposed by the capacity and productivity of the single-armed robotic systems utilized. We have recently constructed a robotic system aimed at overcoming this particular problem. This system uses purpose-built microplate stacker units that provide high-capacity microplate storage and, importantly, provide an easy and fast interface between the robotic system and the human operators. This paper describes this automation project and the benefits that have resulted from its deployment.

scholarly journals High Throughput Printing of Two-Dimensional Materials into Wafer-scale Three-dimensional Architectures

2021 ◽  
Author(s):  
Xuan Wei ◽  
Chia-Ching Lin ◽  
Christine Wu ◽  
Ang-Yu Lu ◽  
Nadeem Qaiser ◽  
...  
Keyword(s):  
High Throughput ◽  
Transition Metal Dichalcogenides ◽  
Three Dimensional ◽  
High Capacity ◽  
Building Blocks ◽  
High Rate ◽  
Wearable Electronics ◽  
Two Dimensional ◽  
Critical Feature ◽  
Wafer Scale

Abstract Architected materials that actively respond to external stimuli hold tantalizing prospects for applications in energy storage, harvesting, wearable electronics and bioengineering. Transition metal dichalcogenides (TMDs) which represent the three-atom-thick, two-dimensional (2D) building blocks, are excellent candidates but have found limited success compared to metallic, inorganic, and organic counterparts due to the lack of up-scalable manufacturing. Here we report the high-throughput printing of 2D TMDs into wafer-scale 3D architectures with structural hierarchy across seven orders of magnitude between critical feature sizes. Anode made of 3D MoS2 architectures comprises the concentric vortex-like intricacy that unites technological merits from architecture, mechanical engineering, and electrochemistry not found in its bulk or exfoliated/epitaxy counterparts. The result is, contrary to expectation, the high-rate, high-capacity, and high-loading lithium (Li)-storage, surpassing those state-of-the-art anode designs while the technique offers an evaporation-like simplicity for industrial scalability.

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