Effects of Yimu Shenghuasan Preparation on the Cytochrome P450 in Endometrial Cells and Immune Function of Dairy Cows

Postpartum uterine infections affect ovarian function and delay ovulation in cattle. As dietary fats can affect immune cell function, we investigated the influence of prepartum diets on postpartum uterine inflammatory status (UIS) as assessed 25 ± 1 days postpartum by endometrial cytology (normal: ≤8% polymorphonuclear cells (PMN) vs subclinical endometritis (SCE): >8% PMN) and associations between SCE, pro- and anti-inflammatory cytokine gene expression and ovarian function. During the last 5 weeks of gestation, dairy cows received a diet supplemented with 8% rolled sunflower (n = 10) or canola seed (n = 9) or no oilseed (n = 9). Ovaries were scanned until 35 days postpartum. Prepartum diets did not influence SCE, but a preovulatory-size follicle developed sooner (P ≤ 0.05), the interval to first ovulation was shorter and the proportion of cows ovulating within 35 days postpartum was greater in the sunflower seed group. Although mRNA expression of cytokines was not affected by diet, cows with SCE had higher (P ≤ 0.05) expression of interleukin-1β (IL1B), interleukin-8 (CXCL8), IL10 and tumour necrosis factor-α (TNF) than normal cows. The interval (mean ± s.e.m.) from calving to preovulatory-size follicle was shorter (P ≤ 0.05) in normal (13.2 ± 0.9 days) than SCE cows (18.7 ± 1.4 days). In summary, a prepartum diet supplemented with sunflower seed positively influenced postpartum ovarian function without affecting UIS or pro- and anti-inflammatory cytokine gene expression in endometrial cells.

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Endocrine and physiological events from ovulation to establishment of pregnancy in cattle

BSAP Occasional Publication ◽

10.1017/s0263967x00033607 ◽

2001 ◽

Vol 26 (1) ◽

pp. 81-91 ◽

Cited By ~ 1

Author(s):

W.W. Thatcher ◽

M. Binelli ◽

D. Arnold ◽

R. Mattos ◽

L. Badinga ◽

...

Keyword(s):

Dairy Cows ◽

Post Partum ◽

Secretory Response ◽

Embryo Survival ◽

Endometrial Cells ◽

Stat Proteins ◽

In Vivo Experiments ◽

Feeding Diets

AbstractA series of in vitro and in vivo experiments were conducted to characterise the dialogue between embryo and maternal units relative to the mechanisms controlling embryo survival in dairy cattle. Endometrial explants from pregnant cows had an attenuated PGF2α secretory response following treatment with melittin (stimulator of PLA2) and phorbol 12, 13 dibutyrate (PDBu). Thus previous exposure to the conceptus appears to regulate the endometrial synthetic pathway at a point coincident with or distal to PLA2 as well as inhibit PKC or PKC mediated events. Endometrial explants collected from cows receiving intrauterine infusions of rblFN-τ had a reduced secretory response following stimulation with PDBu indicating attenuation in PKC activity. Based upon tyrosine-phosphorylation of STAT-proteins and their translocation to the nucleus after treatment with rbIFN-τ, the JAK-STAT pathway is functional in immortalised bovine endometrial cells (BEND cells). Bend cells, exposed to rblFN-τ, reduced PDBu induction of PGF2α secretion and also decreased protein expression of Cox-2 and PLA. RblFN-τ clearly reduced PKC mediated events leading to an antiluteolytic response in endometrial cells. Feeding diets containing 2.6, 5.2 and 7.8% Menhaden fish meal to lactating dairy cows reduced uterine secretion of PGF2α following sequential injections of oestradiol and oxytocin. Thus antiluteolytic effects in early pregnancy may be amplified by feeding by-pass fats. Pregnancy rate to a timed insemination at first service post-partum is increased in association with injection of bST(500 mg; sc) given at insemination. Furthermore injection of bST at time of insemination in superovulated donor cows increased the number of blastocysts and reduced number of unfertilised embryos. Prospects of integrating novel strategies to improve embryo development and survival into reproductive management systems appear to be attainable in high producing dairy cows.

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Chromium yeast alleviates heat stress by improving antioxidant and immune function in Holstein mid-lactation dairy cows

Animal Feed Science and Technology ◽

10.1016/j.anifeedsci.2020.114635 ◽

2020 ◽

Vol 269 ◽

pp. 114635

Author(s):

Q. Shan ◽

F.T. Ma ◽

Y.H. Jin ◽

D. Gao ◽

H.Y. Li ◽

...

Keyword(s):

Heat Stress ◽

Immune Function ◽

Dairy Cows ◽

Chromium Yeast

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Effects of Photoperiod During the Dry Period on Cellular Immune Function of Dairy Cows

Journal of Dairy Science ◽

10.3168/jds.s0022-0302(04)73507-9 ◽

2004 ◽

Vol 87 (11) ◽

pp. 3683-3689 ◽

Cited By ~ 31

Author(s):

T.L. Auchtung ◽

J.L. Salak-Johnson ◽

D.E. Morin ◽

C.C. Mallard ◽

G.E. Dahl

Keyword(s):

Immune Function ◽

Dairy Cows ◽

Dry Period ◽

Cellular Immune Function ◽

Cellular Immune

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Effects of whole flaxseed, raw soybeans, and calcium salts of fatty acids on measures of cellular immune function of transition dairy cows

Journal of Dairy Science ◽

10.3168/jds.2015-9974 ◽

2016 ◽

Vol 99 (6) ◽

pp. 4590-4606 ◽

Cited By ~ 16

Author(s):

J.R. Gandra ◽

R.V. Barletta ◽

R.D. Mingoti ◽

L.C. Verdurico ◽

J.E. Freitas ◽

...

Keyword(s):

Fatty Acids ◽

Immune Function ◽

Dairy Cows ◽

Calcium Salts ◽

Cellular Immune Function ◽

Transition Dairy Cows ◽

Cellular Immune

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Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.737608 ◽

2021 ◽

Vol 8 ◽

Author(s):

Guanxin Lv ◽

Hai Wang ◽

Jianfa Wang ◽

Shuai Lian ◽

Rui Wu

Keyword(s):

Immune System ◽

Immune Function ◽

Dairy Cows ◽

Respiratory Burst ◽

Inflammatory Responses ◽

Late Onset ◽

Leukemia Virus ◽

Polymorphonuclear Neutrophil ◽

Inflammatory Factors ◽

Provirus Load

Enzootic bovine leukemia is a late-onset, neoplastic infection caused by the bovine leukemia virus (BLV). BLV infection hinders the function of the immune system and induces other diseases, which negatively affects the performance and health of the infected cows. As the first line of defense against invading foreign pathogenic microorganisms, polymorphonuclear neutrophil (PMN) plays a vital role in the immune system of dairy cows. However, research on the effect of BLV infection on the immune function of PMN in dairy cows is scarce. Therefore, this experiment aimed to elucidate the effects and effect mechanisms of BLV infection on the immune function of PMN in dairy cows with different BLV provirus loads by detecting the chemotaxis, migration, adhesion, phagocytosis, respiratory burst function, and the formation of NETs. The experimental results showed that BLV infection had no significant effect on the phagocytosis of PMN but inhibited their migration and respiratory burst function, and the effects were closely related to the BLV provirus load. Under high BLV provirus load, PMN produced large amounts of NETs, chemokine CXCL7, adhesion molecule CD18, and pro-inflammatory factors IL-8 and TNF-α, triggering inflammatory responses, and tissue damage. The results of this study will help reveal the reason why BLV infection causes the high incidence of mammary gland inflammation in dairy cows.

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