LC-MS/MS analysis and pharmacokinetic study on five bioactive constituents of Tanreqing injection in rats

2016 ◽  
Vol 14 (10) ◽  
pp. 769-775 ◽  
Author(s):  
Feng ZHANG ◽  
Liang SUN ◽  
Shou-Hong GAO ◽  
Wan-Sheng CHEN ◽  
Yi-Feng CHAI
Keyword(s):  
Pharmacokinetic Study ◽  
Bioactive Constituents ◽  
Ms Analysis

An integrated platform for a high-throughput pharmacokinetic study of glycosides using a boronic acid-functionalized 96-well glass plate

2019 ◽  
Vol 55 (64) ◽  
pp. 9543-9546
Author(s):  
Ningning Zhao ◽  
Qianqian Gu ◽  
Zhiqiang Liu ◽  
Fengrui Song ◽  
Zifeng Pi ◽  
...  
Keyword(s):  
High Throughput ◽  
Pharmacokinetic Study ◽  
Boronic Acid ◽  
Large Scale ◽  
Glass Plate ◽  
The Novel ◽  
Ms Analysis ◽  
Integrated Platform

The novel Vial@FPBA strategy was established for a large-scale pharmacokinetic study of glycosides, during which glycosides were absorbed into a boronic acid-functionalized 96-well glass plate and directly desorbed for UHPLC-MS/MS analysis.

UPLC–MS–MS Analysis of Baicalin in the Cerebrospinal Fluid of Rabbits: Application to a Pharmacokinetic Study

2008 ◽  
Vol 68 (5-6) ◽  
pp. 463-466 ◽  
Author(s):  
Shao Liu ◽  
Xin-Zhong Li ◽  
Li-Min Xu ◽  
Peng Lei ◽  
Yi-Zeng Liang
Keyword(s):  
Cerebrospinal Fluid ◽  
Pharmacokinetic Study ◽  
Ms Analysis

scholarly journals Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2239 ◽  
Author(s):  
Vandana Gautam ◽  
Sukhmeen Kohli ◽  
Saroj Arora ◽  
Renu Bhardwaj ◽  
Mohsin Kazi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Chemical Composition ◽  
Salmonella Typhimurium ◽  
Antimutagenic Activity ◽  
Current Investigation ◽  
Bioactive Constituents ◽  
Rhododendron Arboreum ◽  
Ms Analysis ◽  
Deoxyribose Degradation

In this era of urbanization and environmental pollution, antioxidants and antimutagens derived from plants are promising safeguards for human health. In the current investigation, we analyzed the antioxidant and antimutagenic effects of the hexane, chloroform, and ethyl acetate fractions of Rhododendron arboreum Sm. leaves and determined their chemical composition. The different fractions inhibited lipid peroxidation, repressed the production of nitric oxide radicals, and prevented deoxyribose degradation. The antimutagenic activity of the leaf fractions was analyzed against 4-nitro-O-phenylenediamine, sodium azide and 2-aminofluorene mutagens in two test strains (TA-98 and TA-100) of Salmonella typhimurium. The experiment was conducted using pre- and co-incubation modes. The best results were obtained in the pre-incubation mode, and against indirect acting mutagen. The presence of a number of bioactive constituents was confirmed in the different fractions by GC-MS analysis. The study reveals the strong antioxidant and antimutagenic activity of R. arboreum leaves. We propose that those activities of R. arboreum might correspond to the combined effect of the phytochemicals identified by GC-MS analysis. To the best of our knowledge, this is the first report on the antimutagenic activity of R. arboreum leaves.

scholarly journals Phyllanthus wightianusMüll. Arg.: A Potential Source for Natural Antimicrobial Agents

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
D. Natarajan ◽  
R. Srinivasan ◽  
M. S. Shivakumar
Keyword(s):  
Fungal Pathogens ◽  
Antimicrobial Agents ◽  
Shigella Flexneri ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Infective Agent

Phyllanthus wightianusbelongs to Euphorbiaceae family having ethnobotanical importance. The present study deals with validating the antimicrobial potential of solvent leaf extracts ofP. wightianus. 11 human bacterial pathogens (Bacillus subtilis,Streptococcus pneumoniae,Staphylococcus epidermidis,Proteus vulgaris,Pseudomonas aeruginosa,Klebsiella pneumoniae,Salmonella typhimurium, Escherichia coli, Shigella flexneri, Proteus vulgaris, andSerratia marcescens) and 4 fungal pathogens (Candida albicans, Cryptococcus neoformans, Mucor racemosus, andAspergillus niger) were also challenged with solvent leaf extracts usingagar well and disc diffusion methods. Further, identification of the active component present in the bioactive extract was done using GC-MS analysis. Results show that all extracts exhibited broad spectrum (6–29 mm) of antibacterial activity on most of the tested organisms. The results highlight the fact that the well in agar method was more effective than disc diffusion method. Significant antimicrobial activity was detected in methanol extract againstS. pneumoniae(29 mm) with MIC and MBC values of 15.62 μg/mL. GC-MS analysis revealed that 29 bioactive constituents were present in methanolic extract ofP. wightianus,of which 9,12-octadecaenioic acid (peak area 22.82%; RT-23.97) and N-hexadecanoic acid (peak area 21.55% RT-21.796) are the major compounds. The findings of this study show thatP. wightianusextracts may be used as an anti-infective agent in folklore medicine.

scholarly journals PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS OF THE HEXANE EXTRACT FROM ACANTHOSPERMUM HISPIDUM (whole plant)

2021 ◽  
Vol 46 (1) ◽  
Author(s):  
Akoh, O. U ◽  
Mac-Kalunta O. M ◽  
Emewusim, C. M
Keyword(s):  
Hexane Extract ◽  
Raw Material ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Bioactive Constituents ◽  
Invasive Weed ◽  
Ms Analysis ◽  
Standard Procedures ◽  
Whole Plant ◽  
Entire Plant

Although Acanthospermum hispidum has long been considered as a competitive invasive weed of agricultural plantations, it has recently become a sought after raw material to manufacture syrups in the treatment of asthma, bronchitis and fever. In the present study, the bioactive components of Acanthospermum hispidium entire plant have been accessed via GC-MS analysis. Six compounds from the hexane extract of the whole plant were identified. The most prevalent are glycerine (100.00%), 1-benzoypiperidine (40.26%) and erythritol (15.79%). Phytochemical screening investigation carried out using standard procedures showed the presence of Alkaloids, Steriods, Saponnins, Tannin, Flavonoids, Phenol, Terpenoids.

scholarly journals GC-MS analysis of the essential oil, aroma components and n-hexane extract of St. John Wort (Hypericum perforatum L., Hypericaceae)

2018 ◽  
Vol 64 (01) ◽  
pp. 63-77
Author(s):  
Arijeta Shabani ◽  
Marija Karapandzova ◽  
Ivana Cvetkovikj Karanfilova ◽  
Gjose Stefkov ◽  
Svetlana Kulevanova
Keyword(s):  
Essential Oil ◽  
Hypericum Perforatum ◽  
Experimental Studies ◽  
Hexane Extract ◽  
Bioactive Constituents ◽  
Specific Chemical ◽  
Ms Analysis ◽  
Hexane Extracts ◽  
Pharmacological Potential ◽  
Aroma Components

St. John Wort (Hypercum perforatum L., Hypericaceae) has been used as a medicinal plant for a long period of time as this plant is characterized by a diversity of bioactive constituents which possess well documented pharmacological activities including antiviral, antimicrobial, anti-inflammatory, antioxidant, hepatoprotective and anti-tumoral activity. Nowadays, special interest is put on its essential oil as some experimental studies showed great biological and pharmacological potential. According this, the main goal of this study was GC/MS analysis of the essential oil, aroma components as well as n-hexane extracts of Hypericum perforatum that grows in Western region in R. Macedonia. GC/FID/MS analyses of the isolated essential oils from leaf, flower and herb resulted in the identification of 84 compounds. The fraction of sesquiterpenes was dominated in all examined oils and the main constituents were germacrene D (17.77-39.03%), E-caryophyllene (11.37-25.71%) and β-selinene (0.69-4.77%). GC/HS/MS analyses of the aroma components resulted in the identification of 23 compounds. Among them, isononane was identified as main aroma component (up to 75%). GC/FID/MS analyses of the n-hexane extracts resulted in the identification of 60 compounds which were characterized by the presence of terpenoid (mono- and sesquiterpene) components and non-terpenoid constituents mainly consisted of hydrocarbons and their oxygenate derivatives and related components. The non-terpenoid fraction represented the largest part of the analysed extracts. The most abundant were nonacosane (15.45-49.28%), octacosane (1.33-40.05%) and pentacosane (1.68-9.04%). The aerial parts of H. perforatum collected from Western part of R. Macedonia could be considered as a good source of essential oil with specific chemical profile as well as aroma components and high lipophilic compounds, but further investigation should be done in accordance to their possible commercial or medicinal use. Keywords: Hypericum perforatum, essential oil, aroma components, n-hexane extract, GC-MS

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