Streptococcus pyogenes and Staphylococcus aureus

2013 ◽  
pp. 316-322
Author(s):  
Stephen B. Gordon ◽  
Eldryd Parry
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
And Staphylococcus Aureus

scholarly journals Nonspecific toxicities of Streptococcus pyogenes and Staphylococcus aureus dCas9 in Chlamydia trachomatis

2019 ◽  
Vol 77 (9) ◽  
Author(s):  
Wurihan Wurihan ◽  
Yehong Huang ◽  
Alec M Weber ◽  
Xiang Wu ◽  
Huizhou Fan
Keyword(s):  
Staphylococcus Aureus ◽  
Chlamydia Trachomatis ◽  
Streptococcus Pyogenes ◽  
Expression System ◽  
Knock Out ◽  
Guide Rna ◽  
Knock Down ◽  
Chromosomal Genes ◽  
Recent Advancement ◽  
And Staphylococcus Aureus

ABSTRACT Chlamydiae are common, important pathogens for humans and animals alike. Despite recent advancement in genetics, scientists are still searching for efficient tools to knock out or knock down the expression of chromosomal genes. We attempted to adopt a dCas9-based CRISPR interference (CRISPRi) technology to conditionally knock down gene expression in Chlamydia trachomatis using an anhydrotetracycline (ATC)-inducible expression system. Surprisingly, expression of the commonly used Streptococcus pyogenes dCas9 in C. trachomatis causes strong inhibition in the absence of any guide RNA (gRNA). Staphylococcus aureus dCas9 also shows strong toxicity in the presence of only an empty gRNA scaffold. Toxicity of the S. pyogenes dCas9 is readily observed with as little as 0.2 nM ATC. Growth inhibition by S. aureus dCas9 is evident starting at 1.0 nM ATC. In contrast, C. trachomatis growth was not affected by methionine-tRNA ligase overexpression induced with 10 nM ATC. We conclude that S. pyogenes and S. aureus dCas9 proteins in their current forms have limited utility for chlamydial research and suggest strategies to overcome this problem.

Expression of the Major Heat Shock Proteins DnaK and GroEL in Streptococcus pyogenes : A Comparison to Enterococcus faecalis and Staphylococcus aureus

2001 ◽  
Vol 42 (4) ◽  
pp. 264-268 ◽  
Author(s):  
Marinella S. Laport ◽  
Angela C.D. de Castro ◽  
Adriana Villardo ◽  
Jose Abelardo C. Lemos ◽  
Maria do Carmo F. Bastos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Shock Proteins ◽  
And Staphylococcus Aureus

scholarly journals Antibacterial Activity of Kaempferia Galanga L. Hard Candy Against Streptococcus pyogenes and Staphylococcus aureus Bacteria Growth

2021 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Maria Belgis ◽  
Ahmad Nafi’ ◽  
Giyarto Giyarto ◽  
Afina Desi Wulandari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Streptococcus Pyogenes ◽  
Volatile Content ◽  
Bacteria Growth ◽  
Kaempferia Galanga ◽  
Oil Concentration ◽  
And Staphylococcus Aureus ◽  
Hard Candy

Aromatic ginger (Kaempferia Galanga L.) is a spice plant that has a high volatile content with active compounds of ethyl p-methoxy cinnamate (23.65%) and ethyl cinnamate (5.98%). These two compounds have been reported to have antibacterial activity against Streptococcus pyogenes and Staphylococcus aureus, which are two types of bacteria that can cause sore throat. The application of aromatic ginger essential oil on hard candy can potentially reduce inflammation in the throat. This study aims to determine the effect of aromatic ginger essential oil concentration on hard candy in inhibiting the growth of Streptococcus pyogenes and Staphylococcus aureus bacteria and to determine their acceptance by the panelists. This research was conducted with 6 treatments of variations in the concentration of aromatic ginger essential oil, including 0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The results showed that the higher the concentration of aromatic ginger essential oil on hard candy would further increase the inhibition of the growth of Streptococcus pyogenes and Staphylococcus aureus bacteria. The highest inhibitory activity and the highest panelist acceptance was 1% essential oil concentration with 3.29% moisture content, 0.12% ash content, and 7.13% reducing sugar. The use of aromatic ginger essential oil on hard candy as a flavoring and well as inhibiting the growth of strep throat-causing bacteria (Streptococcus pyogenes and Staphylococcus aureus) has the potential to be developed. Keywords: Antibacterial, hard candy, aromatic ginger, essential oil, Streptococcus pyogenes, Staphylococcus aureus

scholarly journals Bacteriology and Antibiotic Susceptibility Pattern of Peitonsillar Abscess

2010 ◽  
Vol 49 (178) ◽  
Author(s):  
A Acharya ◽  
R Gurung ◽  
B Khanal ◽  
A Ghimire
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
Clinical Laboratory ◽  
Positive Culture ◽  
Peritonsillar Abscess ◽  
Susceptibility Pattern ◽  
Acute Tonsillitis ◽  
Antibiotic Susceptibility Pattern ◽  
The Common ◽  
And Staphylococcus Aureus

INTRODUCTION: Peritonsillar abscess is a common complication of acute tonsillitis. Its management consists of aspiration or surgical drainage followed by appropriate antibiotics. Appropriate antibiotic treatment depends on the common organisms associated with infection and their antibioticsensitivity pattern in local scenario. METHODS: Pus samples aspirated from diagnosed cases of peritonsillar abscess and submitted for culture and sensitivity in last two years were included in this study. Identification of different microorganisms was made on the basis of the microscopic findings, observation of their colony morphology and standard biochemical reactions. Susceptibility pattern to commonly used antibiotics were determined by Clinical Laboratory Standards Institute (CLSI) guidelines. RESULTS: Altogether 24 pus samples were included in the study. Positive culture was obtained from 18 samples. Among them one organism was isolated from 13 samples whereas from five samples multiple organisms were isolated. From six samples no organisms could be cultured. Altogether Streptococcus pyogenes was isolated from 12 samples and Staphylococcus aureus from five samples. Other isolated organisms included Haemophlilus influenzae, Pseudomonas aeruginosa, Escherichia coli and Enterococcus species. Penicillin was effective for Streptococcus pyogenes whereas Staphylococcus aureus was resistant to it. Cloxacillin was found to be effective for Staphylococcus aureus. Ciprofloxacin and Ceftazidime were found to be effective for both organisms. CONCLUSIONS: Streptococcus pyogenes and Staphylococcus aureus were more commonly associated with peritonsillar abscess. Streptococcus pyogenes were sensitive to penicillin but all Staphylococcus aureus were resistant to it. This fact should be considered in clinical practice for management of peritonsillar abscess. KEYWORDS: Bacteriology, Peritonsillar abscess, Susceptibility pattern.

Prevalence of Microorganisms in Acute Pharyngitis from Throat Swab in a tertiary care hospital

2021 ◽  
Vol 15 (9) ◽  
pp. 2182-2185
Author(s):  
Mumtaz Ahmad Umar ◽  
Mirza Nasheed Baig ◽  
Nighat Arif ◽  
Naveed Arshad ◽  
Ayesha Jawad ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Streptococcus Pyogenes ◽  
Antimicrobial Agents ◽  
Throat Swab ◽  
Tertiary Care ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Acute Pharyngitis ◽  
And Staphylococcus Aureus

Background: Acute pharyngitis is the inflammation of the mucous membrane of oropharynx which is caused by various infectious organisms like bacteria, viruses and fungi. The other less common seen causes include allergies and gastro-esophageal reflux disease. Aim: To look for range of microorganisms involved in causing acute pharyngitis on throat swab specimens. Methods: After getting consent all the pharyngitis patients attending ENT Department at Akbar Niazi Teaching Hospital (ANTH), Islamabad from January to June 2017 were included in the study. From total of 100 patients throat swabs were obtained by established aseptic method and were sent for culture. The organisms involved were recognized by biochemical tests & their propensity to antimicrobial agents was established by established methods. Data was entered and analyzed by using SPSS v.21. Results: Out of 100 specimens’ microorganisms were identified in 25 specimens while 75 specimens had no growth. Streptococcus pyogenes was the frequently seen organism 9(90%), while the list was followed by Staphylococcus aureus 14(56%) and Candida albicans 3(12%). In 40% single pathogen was identified with Streptococcus pyogenes in 9 samples (90%) and Staphylococcus aureus in 1(10%), whereas 60% of the culture reports were that of the mix infection. Conclusion: The study concluded that streptococcus pyogenes and staphylococcus aureus were responsible for majority of single organism infections, while candida albicans together with these bacteria were isolated from mixed infections. Keywords: Pharyngitis; Streptococcus pyogenes; Throat; Microorganisms.

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