Dietary amino acid balance and requirements for pigs weaned at 3 weeks of age

1984 ◽  
Vol 39 (3) ◽  
pp. 441-448 ◽  
Author(s):  
Y. Zhang ◽  
I. G. Partridge ◽  
H. D. Keal ◽  
K. G. Mitchell

ABSTRACTNitrogen balance was measured in a series of experiments to determine the optimum balance between lysine, threonine and methionine plus cystine by successive additions of the free amino acids to a basal diet of barley, soya bean meal and tallow. The basal diet had a calculated digestible energy (DE) value of 14·5 MJ/kg and a crude protein content of 146 g/kg. The ranges of total lysine; threonine and methionine plus cystine tested were (g/kg) 6·6 to 11·8, 4·9 to 6·9 and 4·3 to 71, respectively. Latin-square designs were used involving a total of 33 pigs. Nitrogen utilization was optimized in pigs of 3 to 9 weeks of age when each kg of diet contained 10·5 g lysine, 6·4 g threonine and 5·0 g methionine plus cystine, corresponding to a ratio of 100: 61: 48. Thereafter, five diets were formulated in which the same ratio was maintained, but at different protein levels (129 to 192 g/kg). Again the diets were based on barley, soya bean meal and tallow supplemented with free amino acids. These diets were compared, in both nitrogen balance and performance experiments, with a conventional early-weaning diet with a protein content of 240 g/kg. All six diets had the same calculated DE value (14·5 MJ/kg). The N balance experiment was of Latin-square design and involved 18 pigs, and the performance experiment used 36 pigs fed individually from 3 to 9 weeks of age. A diet containing 0·99 g lysine per MJ DE (14·4 g lysine and 192 g balanced protein per kg) was considered to be adequate on the basis of the growth rate and food conversion efficiency results obtained.

2011 ◽  
Vol 96 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Tapas K. Mandal ◽  
Nargish Parvin ◽  
Santanu Mondal ◽  
Vijaylaxmi Saxena ◽  
Ashok K. Saxena ◽  
...  

1992 ◽  
Vol 54 (2) ◽  
pp. 281-287 ◽  
Author(s):  
F. Gatel ◽  
G. Buron ◽  
J. Fékéte

AbstractTwo experiments were carried out with weaned piglets from 8 to 25 kg live weight in order to determine the dietary amino acid content necessary for maximum growth. Six diets based on wheat, soya-bean meal, soya-bean oil and free amino acids were compared in each experiment. Essential amino acids were in the same relative proportion for all diets: (methionine + cystine)/lysine = 0·60 to 0·65; threonine/lysine = 0·65; tryptophan/lysine = 0·19. The range of amino acid content was 9·53 to 12·52 g lysine per kg in the first experiment and 11·34 to 15·94 g lysine per kg in the second experiment. The number of piglets used per diet was 136 (20 pens) and 106 (16 pens) in respectively the first and the second experiment. The relationship between either dietary lysine content or daily lysine intake and growth rate was quadratic and significant. Dietary lysine content and daily lysine intake which enable maximum growth were calculated according to this model. Dietary lysine contents were 15·5 and 14·9 g/kg for the first 3 weeks (8 to 17 kg) and for the overall post-weaning period (8 to 25 kg) respectively. Daily lysine intakes were 10·6 and 13·3 g/day respectively for the same two periods. Reasons for these values being higher than those currently cited are discussed.


1969 ◽  
Vol 11 (4) ◽  
pp. 553-556 ◽  
Author(s):  
H. F. Hintz ◽  
W. G. Pond ◽  
W. J. Visek

SUMMARYIn trials 1 and 2, supplements of urea and cottonseed meal increased the protein content of the basal diets from 12·2 to 14·0–14·6%, but had no significant effects on growth rate or carcass characteristics. In trial 3 the effect of cottonseed meal on growth rate was almost significant but urea had less effect. In trial 4, the basal diet contained 11·0% protein; soya bean meal increased this to 13·1% and had an almost significant effect on growth rate. Lysine and lysine + urea had no effect.


1986 ◽  
Vol 107 (2) ◽  
pp. 263-272 ◽  
Author(s):  
J. A. Rooke ◽  
P. Alvareza ◽  
D. G. Armstrong

SummaryA 4 x 4 latin-square design experiment was carried out to determine the effects of increasing nitrogen (N) intake by feeding diets containing increasing amounts of soyabean meal upon the digestion of organic matter (OM) and N by cattle equipped with rumen and duodenal cannulae. A basal diet (B) containing 600 g ground barley and 400 g grass silage/kg diet and three diets (BS1, BS2 and BS3) in which increasing amounts of the barley were replaced by soya-bean meal were fed. The mean daily intakes of OM and N when each diet was fed were 4·56, 4·55, 4·30 and 4·52 kg OM and 920, 114·7, 138·3 and 164·1 g N for diets B, BSl, BS2 and BS3 respectively.Neither the amounts of OM entering the small intestine nor those voided in the faeces were altered by the diets fed. Thus the mean apparent OM digestibility for all the diets fed was 0·74 + 0·013 and the proportion of digestible OM intake apparently digested in the rumen was 0·77 + 0048.Mean daily concentrations of ammonia N in the rumen were significantly (P < 0·001) increased from 38 mg N/l (diet B) to 129 mg N/l (diet BS3) as N intake increased.The quantities of non-ammonia N and of amino acid N entering the small intestine were not significantly (P >0·05) increased as more soya-bean meal was added to the diet, although diet BSl supported the greatest flows of N to the small intestine. Thus as more soya-bean meal was added to the diet there were increasing net losses of nonammoniaN(P < 0·01) and amino acid N (P < 0·01) prior to the small intestine. Faecal N excretion was not increased (P > 0·05) as soya-bean meal intake increased and thus apparent N digestibility was significantly (P < 0·01) increased by increasing soyabean intake.Both the quantities of microbial N entering the small intestine daily and the apparent efficiency of microbial N synthesis within the rumen were increased when diet BSl was fed in comparison with the basal diet (B) and then declined when diets BS2 and BS3 were fed; these increases were not significant. The quantities of feed N entering the small intestine daily were not significantly (P > 0·05) increased as soya-bean meal intake increased; thus apparent feed N degradability in the rumen was significantly (P < 0·01) increased as soya-bean meal intake increased. In contrast, the rates of disappearance of N from each of the components of the diets fed, when incubated in the rumens of the cattle in porous synthetic fibre-bags, were not increased (P > 0·05) as soya-bean meal intake increased.


1995 ◽  
Vol 62 (3) ◽  
pp. 423-429 ◽  
Author(s):  
Jai-Jun Choung ◽  
David G. Chamberlain

SummaryTwo experiments were conducted to test the hypothesis that responses of milk production to the abomasal infusion of casein or hydrolysates of casein can be influenced by the proportion of peptide-bound amino acids in the infusate. Eight lactating cows were used in two Latin square experiments with period lengths of 10 d. In Expt 1, the four cows were at a late stage of lactation and the four treatments were a basal diet of silage and a barley–soya supplement alone or with infusions into the abomasum of 180 g/d of sodium caseinate, an enzymic hydrolysate of casein or an acid hydrolysate of casein; all treatments supplied equivalent amounts of all the amino acids, this being achieved by addition of free amino acids as required. As infused, the three treatments contained 89 (caseinate), 40 (enzymic hydrolysate) and 15 (acid hydrolysate) % of their amino acids as peptides. The increase of milk production in response to infusion was small and there were no significant differences between the infusion treatments. In Expt 2, the cows received a basal diet of grass silage and a supplement containing feather meal as the main source of protein. Treatments were the basal diet alone and with three infusion treatments as in Expt 1, except that the amount infused was 230 g/d. The response to infusion was greater in this experiment, there were significant differences between infusions and the yield of milk protein with the infusion treatments was linearly related (P = 0·001) to the proportion of peptide-bound amino acids in the infusate. Moreover, there was a close inverse relation (P = 0·007) between the proportion of peptide-bound amino acids in the infusate and the concentration of total peptidebound amino acids in blood plasma.


1987 ◽  
Vol 44 (3) ◽  
pp. 415-420 ◽  
Author(s):  
I. G. Partridge ◽  
A. G. Low ◽  
J. J. Matte

ABSTRACTIn order to determine the relative nutritional value of rapeseed meal (var. Tandem) in comparison with soya-bean meal and fish meal, seven barley-based diets were formulated to provide 13·1 MJ digestible energy and 8·8 g total lysine per kg. They contained soya-bean meal (S), rapeseed meal (R) or fish meal (F) alone, or equal proportions (on a total nitrogen basis) of soya and rapeseed, soya and fish meal, rapeseed and fish meal or soya, rapeseed and fish meal. The study involved 14 pigs growing from 40 to 90 kg, each fitted with a T cannula in the terminal ileum. Dietary treatments were applied during 7-day periods according to a 7 × 7 Latin-square design, replicated twice. The pigs were fed at 12-h intervals. Ileal digesta were collected during two 12-h periods from each pig after adaptation to each diet. Nutrient apparent digestibilities were measured using chromium III oxide as a marker; for diets S, R and F, respectively, values were: dry matter, 0·58, 0·51 and 0·66 (s.e. 0·014); N, 0·72, 0·67 and 0·73 (s.e. 0·011); lysine, 0·79, 0·72 and 0·83 (s.e. 0·010), with a similar relationship between diets for most other amino acids. In general, values for diets containing combinations of the protein supplements were intermediate between those for diets containing the respective single supplements. Following the main trial, seven pigs were given a semi-purified diet containing rapeseed meal as the only protein source; this gave amino acid digestibility values similar to or slightly higher than diet R. Diet R, which contained 375 g rapeseed meal per kg, presented no palatability problem. Rapeseed meal of the quality used in this trial could contribute a substantial proportion of the protein supplement for growing pigs. Its value should be enhanced by formulating diets according to the ileal digestibility of the limiting amino acids.


1960 ◽  
Vol 55 (2) ◽  
pp. 215-223 ◽  
Author(s):  
B. Laksesvela

1. The potency of balancing interactions between the protein of herring press-cake (dried) and solubles (condensed) on one side, and certain vegetable feeds on the other, has been examined on 2- to 4-week-old chicks:(a) Initially by comparing the co-effect of a series of different combinations of the herring products when ingested together with certain cereals plus extracted ground-nut meal, to that estimated earlier when the same combinations of herring products formed the sole dietary protein.(b) Thereafter by studying whether the co-effect of the herring products was influenced or not by substituting extracted soya-bean meal for the ground-nut meal.(c) Further by tentative trials to show that registered interactions were caused by some keystone amino acids.2. A special dietary system was devised for the purpose (a basal diet of actual feeds + a synthetic, practically protein-free basal diet + the tested feed, eventually + adjustments), the results being recorded as combinative protein value = c.p.v.3. Interactions between dietary constituents not only were shown under the circumstances, but proved to be an easily acting factor of great power. Herring solubles in proportions of 15–45% were formerly found to bring about a statistically significant improvement of the meal when fed together with this as the only protein of the diet. But: (a) the introduction of certain cereals and ground-nut readily converted the beneficial effect of the solubles into a significantly negative one. (b) Whereas soya-beans instead of ground-nut evidently reversed the situation again. In this third grouping solubles at moderate levels appeared neutral, meaning that herring solubles combined significantly better with soya-bean than with ground-nut when fed to young chicks in presence of a set of cereals and herring meal, (c) Responses to supplemental, crystalline amino acids showed that the interactions between the protein sources could be attributed to their constituent amino acids. Thus the little efficient combination of solubles and ground-nut plus certain cereals could be greatly improved by small quantities of all ten essential amino acids together, and equally much by lysine plus threonine only. Contrarily, the diet appeared weakened when lysine, threonine or isoloucine were omitted singly or together from the whole ten. The same was the case with single addition of leucine.4. The discussion stresses the pre-eminent importance of the diet in biological estimations of protein value. It also points out the practical significance of skilful combination of different protein sources.


1968 ◽  
Vol 10 (3) ◽  
pp. 311-318 ◽  
Author(s):  
P. J. Davies

Energy intake and nitrogen balance studies have been conducted on 12 lambs fed ad libitum on four all-concentrate diets of equal crude-protein content in an experiment of latin square design. Each diet contained one cereal, either barley or oats and one protein supplement, either white-fish meal or soya bean meal.Protein supplements had no influence on energy intake, but intake was significantly higher when the lambs were given barley rather than oat diets.There were highly significant differences in the degree of utilization of digested nitrogen between diets containing fish meal and soya bean meal, and between diets containing barley and oats.The energy: nitrogen ratio of urine was inversely related to the proportion of urinary nitrogen present as urea and directly related to the degree of utilization of digested nitrogen.


1995 ◽  
Vol 62 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Jai-Jun Choung ◽  
David G. Chamberlain

SummaryThe effects of the form in which amino acids are presented to the abomasum on the milk production of dairy cows receiving a basal diet of grass silage and a barley-based supplement were examined in two experiments. Effects of abomasal infusions of sodium caseinate were compared with the effects of corresponding levels of either an enzymic hydrolysate of casein (Expt 1) or a corresponding mixture of free amino acids (FAA; Expt 2). In Expt 1, although the yield of protein in milk increased progressively with each level of infusion, the yields of protein were greater for the caseinate than for the hydrolysate. Again, in Expt 2, for milk protein yield, sodium caseinate was superior to FAA at the lower level of infusion. In both experiments, the hydrolysate and FAA treatments were associated with higher concentrations of fat in the milk. There were indications of differences in the pattern of secretion of glucagon between the caseinate and FAA treatments. It is concluded that the differences between treatments relate either to the kinetics of absorption of amino acid residues or to the action of bioactive peptides released during digestion of casein.


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