Dietary red palm oil reduces ischaemia–reperfusion injury in rats fed a hypercholesterolaemic diet

We have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia–reperfusion. However, little knowledge exists concerning the effects of RPO supplementation of a high-cholesterol diet on ischaemia–reperfusion injury. The signalling mechanisms responsible for RPO's effects in the presence of cholesterol also remain to be elucidated. Therefore, the aim of the present study was to examine the effects of RPO, given with a high-cholesterol diet, on mitogen-activated protein kinase (MAPK) phosphorylation and apoptosis. Long–Evans rats were fed a control diet, a control diet containing 2 % cholesterol, or a control diet containing 2 % cholesterol and 7 g RPO per kg (CRPO) for 5 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured after which hearts were freeze-clamped and used to assess MAPK phosphorylation and to evaluate apoptosis. Cholesterol supplementation caused a poor aortic output (AO) recovery compared with the control group (35·5 (sem 6·2) v. 55·4 (sem 2·5) %), but when RPO was added, the percentage AO increased significantly. The cholesterol group's poor AO was associated with a significant increase in p38-MAPK phosphorylation, whereas the CRPO-supplemented group showed as significant reduction in p38-MAPK phosphorylation when compared with the cholesterol-supplemented group. This significant reduction in p38-MAPK was also associated with reduced apoptosis as indicated by significant reductions in caspase-3 and poly(ADP-ribose) polymerase cleavage.

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Dietary red palm oil improves reperfusion cardiac function in the isolated perfused rat heart of animals fed a high cholesterol diet

Prostaglandins Leukotrienes and Essential Fatty Acids ◽

10.1016/j.plefa.2004.10.014 ◽

2005 ◽

Vol 72 (3) ◽

pp. 153-161 ◽

Cited By ~ 19

Author(s):

A.J. Esterhuyse ◽

E.F. du Toit ◽

A.J.S. Benadè ◽

J. van Rooyen

Keyword(s):

Cardiac Function ◽

Palm Oil ◽

Rat Heart ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Perfused Rat Heart ◽

Red Palm Oil ◽

Isolated Perfused Rat Heart

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Icariin Attenuates High-cholesterol Diet Induced Atherosclerosis in Rats by Inhibition of Inflammatory Response and p38 MAPK Signaling Pathway

Inflammation ◽

10.1007/s10753-015-0242-x ◽

2015 ◽

Vol 39 (1) ◽

pp. 228-236 ◽

Cited By ~ 27

Author(s):

Yanwu Hu ◽

Bo Sun ◽

Kai Liu ◽

Mengtong Yan ◽

Yang Zhang ◽

...

Keyword(s):

Inflammatory Response ◽

Signaling Pathway ◽

P38 Mapk ◽

Mapk Signaling ◽

Mapk Signaling Pathway ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol

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Influence of crocetin on high-cholesterol diet induced atherosclerosis in rats via anti-oxidant activity together with inhibition of inflammatory response and p38 MAPK signaling pathway

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2016.11.005 ◽

2018 ◽

Vol 25 (3) ◽

pp. 493-499 ◽

Cited By ~ 9

Author(s):

Shu-Ling Diao ◽

Jing-Wu Sun ◽

Bao-Xin Ma ◽

Xiao-Mei Li ◽

Dong Wang

Keyword(s):

Inflammatory Response ◽

Signaling Pathway ◽

P38 Mapk ◽

Mapk Signaling ◽

Mapk Signaling Pathway ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Anti Oxidant

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Lipoprotein lipase and hepatic lipase activities in a hypercholesterolaemic (RICO) strain of rat. Effect of dietary cholesterol

Biochemical Journal ◽

10.1042/bj2660349 ◽

1990 ◽

Vol 266 (2) ◽

pp. 349-353 ◽

Cited By ~ 12

Author(s):

F Sultan ◽

L E Cardona-Sanclemente ◽

D Lagrange ◽

C Lutton ◽

S Griglio

Keyword(s):

Lipoprotein Lipase ◽

Hepatic Lipase ◽

Control Diet ◽

Dietary Cholesterol ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

Cholesterol Feeding ◽

High Cholesterol ◽

Rate Limiting

Hepatic lipase (HL) and lipoprotein lipase (LPL) were assayed in heparinized plasma from male normocholesterolaemic (SW) and genetically hypercholesterolaemic (RICO) rats. Both strains were fed on either a semi-purified control diet or the same diet enriched with 0.5% or 1% cholesterol. HL activity was similar in both groups of rats fed on the control diet. LPL activity was found to be significantly lower in RICO rats (35% decrease, P less than 0.05). Feeding with a high-cholesterol diet led to a decrease in HL activity (15-23%) in both groups of rats but no change was detected in LPL activity, which remained consistently lower in the RICO rats. Thus, with the control diet, LPL activity is lower in RICO rats but presumably is not rate-limiting for their triacylglycerol clearance, given the normal triacylglycerol levels present. After cholesterol feeding, however, the lower LPL activity may become rate-limiting together with the decrease in HL activity, as in these circumstances hypertriacylglycerolaemia was evident and the hypercholesterolaemia of this strain was further increased.

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Sweet Potato Leaf Feeding Decreases Cholesterol, Oxidative Stress and Thrombosis Formation in Syrian Hamsters with a High-Cholesterol Diet

Life ◽

10.3390/life11080802 ◽

2021 ◽

Vol 11 (8) ◽

pp. 802

Author(s):

Hao-Hsiang Chang ◽

Yi-Chan Lan ◽

Shiu-Dong Chung ◽

Chiang-Ting Chien

Keyword(s):

Total Cholesterol ◽

Sweet Potato ◽

Control Diet ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Atherogenic Index ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Potato Leaf

Nutritional strategies to reduce hyperlipidemia and the risk of cardiovascular disease are gaining more public favor and medical professionals’ attention. The authors of this study explored the effect of sweet potato leaf powder (SPLP) feeding on the parameters of plasma lipids, reactive oxygen species, and time to thrombosis formation in Syrian hamsters fed with high-cholesterol diets. The animals were separated into six groups: a feeding control diet, a control diet containing 0.1% cholesterol, a control diet containing 0.2% cholesterol, a control diet containing 0.1% cholesterol plus 2.5% SPLP, a control diet containing 0.1% cholesterol plus 5% SPLP, and a control diet containing 0.2% cholesterol plus 5% SPLP for six weeks. The levels of serum total cholesterol (51% increase), low-density lipoprotein cholesterol (70.6% increase), very-low-density lipoprotein cholesterol (51.3% increase), and the triglyceride and atherogenic index (LDL-C/HDL-C) significantly increased in the high-cholesterol diet groups. Concomitant 5% sweet potato leaf powder ingestion significantly decreased the lipid profiles, with a 20.6% total cholesterol reduction in the 0.1% cholesterol diet groups, a 17.2% reduction in the 0.2% group, a 48.7% LDL reduction in the 0.1% cholesterol group, and a 30.3% reduction in the 0.2% group, with a consequent decrease in the atherogenic index. SPLP feeding was found to be associated with increased fecal sterol contents, with a 188.6% increase in the 0.1% cholesterol-fed group and a 177.3% increase in the 0.2% group. The SPLP-fed groups had depressed ROS levels, elongated FeCl3-induced times to thrombosis formation, and increased liver superoxide dismutase contents and SREBP-1 protein expression. Sweet potato leaf intake could reduce plasma total cholesterol, LDL, and oxidative stress. We suggest sweet potato leaf intake as a choice of nutritional strategy for hyperlipidemia and cardiovascular disease prevention.

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