scholarly journals Changes in white muscle transcriptome induced by dietary energy levels in two lines of rainbow trout (Oncorhynchus mykiss) selected for muscle fat content

2009 ◽  
Vol 103 (5) ◽  
pp. 629-642 ◽  
Author(s):  
Catherine-Ines Kolditz ◽  
Elisabeth Plagnes-Juan ◽  
Edwige Quillet ◽  
Florence Lefèvre ◽  
Françoise Médale
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Energy Levels ◽  
High Energy ◽  
Fat Content ◽  
Fat Deposition ◽  
Dietary Energy ◽  
Expression Change ◽  
Vldl Receptor

Energy intake and genetic background are major determinants of muscle fat content in most animals, including man. We combined genetic selection and dietary energy supply to study the metabolic pathways involved in genetic and nutritional control of fat deposition in the muscle of rainbow trout (Oncorhynchus mykiss). Two experimental lines of rainbow trout, selected for lean (L) or fat (F) muscle, were fed with diets containing either 10 or 23 % lipids from the first feeding, up to 6 months. At the end of the trial, trout exhibited very different values of muscle fat content (from 4·2 to 10·1 % wet weight). Using microarrays made from a rainbow trout multi-tissue cDNA library, we analysed the molecular changes occurring in the muscle of the two lines when fed the low-energy or high-energy diet. The results from microarray analysis revealed that eleven metabolism-related genes were differentially expressed according to the diet while selection resulted in expression change for twenty-six genes. The most striking observation was the increased level of transcripts encoding the VLDL receptor and fatty acid translocase/CD36 following both the high-fat diet and upward selection for muscle fat content, suggesting that these two genes are relevant molecular markers of fat deposition in the white muscle of rainbow trout.

Effect of postweaning implantation of zeranol and dietary energy level on growth and reproductive performance of replacement beef heifers

1991 ◽  
Vol 71 (2) ◽  
pp. 265-270 ◽  
Author(s):  
M. Makarechian ◽  
P. F. Arthur ◽  
M. A. Price
Keyword(s):  
Reproductive Performance ◽  
Energy Requirement ◽  
Energy Levels ◽  
Reproductive Traits ◽  
High Energy ◽  
Dietary Energy ◽  
Maintenance Energy Requirement ◽  
And Control ◽  
Calving Difficulty ◽  
Effect On Growth

Eighty-one heifer calves weaned at an average age of 6 mo were used to study the effect of postweaning zeranol implantation, on the growth and reproductive performance of heifers fed to grow slowly on three dietary energy levels. Forty heifers were implanted with 36 mg of zeranol at 30 d and again at 120 d postweaning. The remaining heifers were used as ummplanted controls. Within the implanted and control groups, heifers were subdivided into three groups and fed either a low, medium or high energy diet, which provided approximately 105, 130 and 160%, respectively, of NRC maintenance energy requirement, until 1 yr of age. The heifers were then turned out to pasture with hay supplementation. Heifers were exposed to bulls for 35 d at approximately 14 mo of age. Zeranol had no significant effect on growth. The effect of zeranol on the reproductive traits studied was not significant, although the trend was that the zeranol implanted heifers had lower calf crop born (52.3 vs. 63.6%) and lower incidence of calving difficulty (18.5 vs. 32.1%) than control heifers. The level of energy in the diet had a significant (P < 0.01) effect on growth of the heifers, with those on the high energy diet having the highest mean growth rate, but no significant effect on reproductive performance. Key words: Zeranol, dietary energy, growth, reproduction, heifers

scholarly journals Dietary methionine availability affects the main factors involved in muscle protein turnover in rainbow trout (Oncorhynchus mykiss)

2014 ◽  
Vol 112 (4) ◽  
pp. 493-503 ◽  
Author(s):  
Ikram Belghit ◽  
Sandrine Skiba-Cassy ◽  
Inge Geurden ◽  
Karine Dias ◽  
Anne Surget ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Muscle Protein ◽  
Muscle Growth ◽  
Mrna Translation ◽  
Fish Feed ◽  
Mrna Levels ◽  
Methionine Deficiency ◽  
Main Factors

Methionine is a limiting essential amino acid in most plant-based ingredients of fish feed. In the present study, we aimed to determine the effect of dietary methionine concentrations on several main factors involved in the regulation of mRNA translation and the two major proteolytic pathways (ubiquitin–proteasome and autophagy-lysosomal) in the white muscle of rainbow trout (Oncorhynchus mykiss). The fish were fed for 6 weeks one of the three isonitrogenous diets providing three different methionine concentrations (deficient (DEF), adequate (ADQ) and excess (EXC)). At the end of the experiment, the fish fed the DEF diet had a significantly lower body weight and feed efficiency compared with those fed the EXC and ADQ diets. This reduction in the growth of fish fed the DEF diet was accompanied by a decrease in the activation of the translation initiation factors ribosomal protein S6 and eIF2α. The levels of the main autophagy-related markers (LC3-II and beclin 1) as well as the expression of several autophagy genes (atg4b, atg12 l, Uvrag, SQSTM1, Mul1 and Bnip3) were higher in the white muscle of fish fed the DEF diet. Similarly, the mRNA levels of several proteasome-related genes (Fbx32, MuRF2, MuRF3, ZNF216 and Trim32) were significantly up-regulated by methionine limitation. Together, these results extend our understanding of mechanisms regulating the reduction of muscle growth induced by dietary methionine deficiency, providing valuable information on the biomarkers of the effects of low-fishmeal diets.

RNA/DNA ratios in white muscle as estimates of growth in rainbow trout held at different temperatures

1990 ◽  
Vol 68 (7) ◽  
pp. 1494-1498 ◽  
Author(s):  
Moira M. Ferguson ◽  
Roy G. Danzmann
Keyword(s):  
Growth Rate ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Specific Growth ◽  
Fish Size ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Different Temperatures ◽  
Specific Growth Rates ◽  
Rna And Dna

The concentrations of RNA, DNA, and protein in white muscle from 240 uniquely tagged rainbow trout (Oncorhynchus mykiss) held at three temperatures (5, 8 (control), and 11 °C) were measured. Both RNA and RNA/DNA ratios were better predictors of recent length- and weight-specific growth rates than they were of absolute fish size. Furthermore, RNA concentrations were better predictors of growth than RNA/DNA ratios. The strength of the regression between either RNA/DNA ratio or RNA and growth rate did not differ consistently among temperatures. Fish reared at warmer temperatures had lower concentrations of RNA for both a given growth rate and a given DNA concentration compared with cold-reared trout. Warm-reared fish also had lower concentrations of DNA and higher protein/DNA ratios than cold-reared trout when fish size was standardized. The concomitant decrease in both RNA and DNA concentrations resulted in marginally lower RNA/DNA ratios in warm-reared fish.

scholarly journals Ruminal Microbiota and Fermentation in Response to Dietary Protein and Energy Levels in Weaned Lambs

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 109 ◽  
Author(s):  
Xiaokang Lv ◽  
Kai Cui ◽  
Minli Qi ◽  
Shiqin Wang ◽  
Qiyu Diao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Energy Levels ◽  
High Energy ◽  
Metabolizable Energy ◽  
Low Energy ◽  
Dietary Energy ◽  
Ruminal Fermentation ◽  
Protein Levels ◽  
Energy Group ◽  
Low Energy Diet

Supplying sufficient nutrients, such as dietary energy and protein, has a great effect on the growth and rumen development of ruminants. This study was conducted to evaluate the effects of dietary energy and protein levels on growth performance, microbial diversity, and structural and physiological properties of the rumen in weaned lambs. A total of 64 two-month-old Hu lambs were randomly allotted to 2 × 2 factorial arrangements with four replicates and with four lambs (half male and half female) in each replicate. The first factor was two levels of dietary metabolizable energy (ME) density (ME = 10.9 MJ/Kg or 8.6 MJ/Kg), and the second factor was two levels of dietary crude protein (CP) content (CP = 15.7% or 11.8%). The trial lasted for 60 days. A low dietary energy level restrained the growth performance of lambs (p < 0.05). The ruminal concentration of acetate and the ratio of acetate to propionate increased but the propionate concentration decreased significantly with the low energy diet. However, the rumen morphology was not affected by the diet energy and protein levels. Moreover, a low energy diet increased ruminal bacterial diversity but reduced the abundance of the phylum Proteobacteria (p < 0.05) and genus Succinivibrionaceae_uncultured (p < 0.05), which was associated with the change in ruminal fermentation phenotypes. By indicator species analysis, we found three indicator OTUs in the high energy group (Succinivibrionaceae_uncultured, Veillonellaceae_unclassified and Veillonellaceae_uncultured (p < 0.01)) and two indicator OTUs in the low energy group (Bacteroidales_norank and Lachnospiraceae_uncultured (p < 0.01)). In conclusion, these findings added new dimensions to our understanding of the diet effect on rumen microbial community and fermentation response, and are of great significance for establishing the optimal nutrient supply strategy for lambs.

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