scholarly journals Ruminal Microbiota and Fermentation in Response to Dietary Protein and Energy Levels in Weaned Lambs

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 109 ◽  
Author(s):  
Xiaokang Lv ◽  
Kai Cui ◽  
Minli Qi ◽  
Shiqin Wang ◽  
Qiyu Diao ◽  
...  

Supplying sufficient nutrients, such as dietary energy and protein, has a great effect on the growth and rumen development of ruminants. This study was conducted to evaluate the effects of dietary energy and protein levels on growth performance, microbial diversity, and structural and physiological properties of the rumen in weaned lambs. A total of 64 two-month-old Hu lambs were randomly allotted to 2 × 2 factorial arrangements with four replicates and with four lambs (half male and half female) in each replicate. The first factor was two levels of dietary metabolizable energy (ME) density (ME = 10.9 MJ/Kg or 8.6 MJ/Kg), and the second factor was two levels of dietary crude protein (CP) content (CP = 15.7% or 11.8%). The trial lasted for 60 days. A low dietary energy level restrained the growth performance of lambs (p < 0.05). The ruminal concentration of acetate and the ratio of acetate to propionate increased but the propionate concentration decreased significantly with the low energy diet. However, the rumen morphology was not affected by the diet energy and protein levels. Moreover, a low energy diet increased ruminal bacterial diversity but reduced the abundance of the phylum Proteobacteria (p < 0.05) and genus Succinivibrionaceae_uncultured (p < 0.05), which was associated with the change in ruminal fermentation phenotypes. By indicator species analysis, we found three indicator OTUs in the high energy group (Succinivibrionaceae_uncultured, Veillonellaceae_unclassified and Veillonellaceae_uncultured (p < 0.01)) and two indicator OTUs in the low energy group (Bacteroidales_norank and Lachnospiraceae_uncultured (p < 0.01)). In conclusion, these findings added new dimensions to our understanding of the diet effect on rumen microbial community and fermentation response, and are of great significance for establishing the optimal nutrient supply strategy for lambs.

1998 ◽  
Vol 78 (4) ◽  
pp. 533-541 ◽  
Author(s):  
I. B. Mandell ◽  
E. A. Gullett ◽  
J. W. Wilton ◽  
O. B. Allen ◽  
R. A. Kemp

Forty-eight Hereford and 60 Simmental steers were used to evaluate breed differences as affected by dietary energy content on growth performance, carcass and chemical composition, and beef quality. Diets were based on corn silage, alfalfa haylage, whole corn, and SBM and were formulated to provide 2.52 to 2.81 Mcal kg−1 ME and 11.7 to 12.6% protein in the growing phase and 2.69 to 2.86 Mcal kg−1 ME and 9.7 to 10.4% protein in the finishing phase. Low- and high-ener-gy diets were formulated for each breed with the high-energy diet for Hereford serving as the low-energy diet for Simmental. Steers were slaughtered after attaining 8 to 10 mm backfat determined by ultrasound. Higher energy diets increased (P < 0.09) average daily gain (ADG) and feed efficiency within both breeds. Hereford gained more rapidly (P = 0.074) and were more (P = 0.001) efficient in converting feed to gain than Simmental. High-energy diets decreased (P = 0.001) days on feed for both breeds and increased (P = 0.001) carcass weights for Simmental. Otherwise, carcass and chemical composition were generally unaffected by dietary energy content. Simmental were heavier (P = 0.001) and leaner (P = 0.001) than Hereford while marbling classification and intramuscular fat content were similar (P > 0.10) between breeds. While shear force decreased (P = 0.043) feeding the low-energy diet to Simmental, other shear and palatability attributes were unaffected by dietary energy content or breed. Altering dietary energy content for Hereford and Simmental influenced growth performance without affecting carcass and chemical composition and beef quality. Key words: Hereford, Simmental, marbling, shear, palatability, growth performance, carcass


1959 ◽  
Vol 52 (3) ◽  
pp. 364-368 ◽  
Author(s):  
W. Bolton

1. The efficiency of food conversion by pullets and yearlings fed on high- and low-energy diets has been determined.2. Egg production was slightly higher by birds fed the low-energy diet for both pullets and yearling hens; pullets laid more eggs than yearling hens.3. For each age, the gross food intake was greater for birds fed the low-energy diet; the consumptions of digestible protein, non-protein digestible energy and metabolizable energy were the same for both diets and both ages of bird.4. The food cost per dozen eggs was slightly greater in the yearling year when the cost of rearing the pullets was ignored, and about the same when it was included.5. The efficiency of utilization of digestible energy and protein showed only a slight decrease from the pullet to the yearling year.6. The variation in the composition and digestibility of eighteen consecutive mixes of the highenergy diet and twenty-five of the low-energy diet was: crude protein 2%, non-protein digestible energy 5% and metabolizable energy 0·4 kg. cal./g. The digestibility of the crude protein and oil was 87%, for both diets; carbohydrates were 81% digestible in the high-energy diet and 59% in the low-energy diet.


2020 ◽  
Vol 100 (4) ◽  
pp. 631-639
Author(s):  
S. Serpunja ◽  
I.H. Kim

A total of 90 finishing pigs [(Yorkshire × Landrace) × Duroc] with an average body weight (BW) of 50.02 ± 1.78 kg were used in a 10 wk experiment. The pigs were distributed into three dietary treatments replicated six times with five pigs (two barrows and three gilts) per pen. The treatment diets were a positive control (PC; high-energy diet), a negative control (NC; low-energy diet), and an NC + 0.05% ferritin diet (TRT1). The supplementation of ferritin in a low-energy diet tended (P = 0.06) to increase the BW at week 5 compared with pigs fed low-energy diets without ferritin. At week 5 and overall period, the gain-to-feed ratio of pigs fed high-energy diets was higher (P < 0.05) compared with pigs fed low-energy diets. The pigs receiving a ferritin-supplemented diet had a comparable growth performance to pigs fed high-energy diets. At week 10, fecal Lactobacilli counts of pigs fed high-energy diets were higher (P < 0.05) compared with pigs fed low-energy diets. The supplementation of low-energy diets with ferritin resulted in comparable growth performance to pigs fed high-energy diets and had no adverse effect on digestibility and fecal gas emissions. Thus, it seems beneficial to include ferritin in low-energy diets of finishing pigs.


2019 ◽  
Vol 6 (2) ◽  
pp. 138 ◽  
Author(s):  
Catootjie Lusje Nalle ◽  
Marlin Rambu Kuba Yowi

High energy diet is related to high feed cost. Thus, it is essential to reduce energy diet but still support the good performance of birds. The utilization of low cost- feed ingredients combined with enzymes are the alternative way to do. The objective of this study was to investigate the effects of multy-enzyme supplementation on growth performance of broilers fed low energy basal diets containing rice bran. Experiment 1. The apparent metabolizable energy (AME) assay of yellow corn and rice bran. A total of 48 broilers were randomly distributed to 12 cages (4 birds/cage). A maize-soybean meal basal diet (mash form) was formulated and two assay diets were then developed by substituting rice bran (raw 250 g kg-1, w/w) and maize (500 g kg-1, w/w) of the basal diet. Total excreta collection method was used. The results showed that the AME value of rice bran was significantly lower (2032 kcal/kg DM) and 3112 Kcal/kg DM. Experiment II. The experiment was designed using a completely randomized design with four treatments and six replications. A total of 160 DOC (unsex) were randomly distributed to 16 pens (10 birds/pen). The experimental diets were iso-nitrogenous and iso-energetics. The results show that dietary treatments significantly affected (P0.05) FCR and mortality of broilers. Broilers fed P2, P3 and P4 diets had higher (P0.05). In conclusion, 1) the supplementation of enzymes in low energy diets containing rice bran improved BWG and FI but not FCR and mortality. 2) Birds fed rice bran-containing-diets supplemented with enzymes produced similar growth performance.


2013 ◽  
Vol 57 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Zhigang Zhang ◽  
Jianguo Wang ◽  
Ruifeng Gao ◽  
Weiqian Zhang ◽  
Xinwei Li ◽  
...  

Abstract The objective of the study was to determine expression of gene of insulin receptor (INSR) in adipose tissue of postpartum dairy cows fed diets containing different amounts of energy at the antepartum period. Healthy pregnant dairy cows (n=45) on 21st d of the antepartum were divided into three groups differing in diet composition, namely: control group fed a normal diet, high energy group fed a high energy diet, and low energy group fed a low energy diet. Twenty-one days after parturition, INSR gene expression in adipose tissue was determined by internally controlled reverse transcriptase PCR. The level of INSR mRNA in adipose tissues of cows fed the high energy diet was substantially lower than that in cows fed normal or low energy diets. A relatively higher level of INSR mRNA in the adipose tissue of cows fed low energy diet may be beneficial for gluconeogenesis and lipogenesis, which can relieve an energy negative balance. Reduced level of INSR mRNA in adipose tissue of cows fed high energy diet indicates that the response to insulin has significantly decreased.


2008 ◽  
Vol 48 (9) ◽  
pp. 1217 ◽  
Author(s):  
A. Y. Abdullah ◽  
F. T. Awawdeh ◽  
H. S. Musallam ◽  
H. H. Titi ◽  
B. S. Obeidat ◽  
...  

Thirty Black goat male kids were used in a 3 × 2 factorial arrangement to evaluate the effects of three dietary energy levels (EL) and early castration (TRT) on growth performance, body and carcass characteristics. Upon weaning at 90 days of age, kids were randomly divided into three equal groups (n = 10: five intact and five castrated) and assigned to one of the three dietary treatments containing 15% CP and different energy levels: low (LEL; 10.44 MJ ME/kg DM), medium (MEL; 11.60 MJ ME/kg DM) and high energy (HEL; 12.90 MJ ME/kg DM). Kids were individually fed on an ad libitum basis and were slaughtered at the end of the fattening period. Results showed no differences between castrated and intact kids in feed and nutrient intake. The level of dietary energy affected dry matter intake (P < 0.05), metabolisable energy intake (P < 0.01), crude protein intake (P < 0.05), neutral detergent fibre (P < 0.001) and acid detergent fibre intakes (P < 0.001). Dry matter intake was 280, 305 and 264 g/kg W0.75.day for kids receiving LEL, MEL and HEL diets, respectively. Metabolisable energy intake was the highest for kids receiving the MEL diet (0.90 Mcal/kg W0.75.day) compared with LEL (0.73 Mcal/ kg W0.75.day) and HEL (0.88 Mcal/kg W0.75.day) diets. Final liveweight and average daily gain (ADG) were greater (P < 0.01) in MEL diets. Kids fed MEL gained 133 g/day, while ADG values were 92 and 100 g/day for kids fed LEL and HEL diets, respectively. Total muscle percentage was greater (P < 0.05) for kids fed MEL diets (55.9%) compared with LEL (53.8%) and HEL (51.1%) diets. Castrated kids had lower (P < 0.01) carcass muscle percentage, but higher carcass (P < 0.01) and subcutaneous (P < 0.001) fat percentages than intact kids. It can be concluded that the best growth performance was observed in kids receiving the MEL with no differences between castrated and intact kids. Castration influenced accretion of fat in castrated kids.


2020 ◽  
Vol 44 (2) ◽  
pp. 25-33
Author(s):  
B. Saleh ◽  
D. J. U. Kalla ◽  
S. T. Mbap ◽  
U. D. Doma ◽  
A. Y. Girgiri

The effects of varying levels of dietary energy and protein on gross morphology and histology of testes of FUNNAB – Alpha chickens were studied at the Abubakar Tafawa Balewa University Bauchi, Bauchi state. Twelve cocks were randomly divided into four dietary treatments; Standard diet (SD) (Control) (2650Kcal/Kg ME/ 16%CP), High Energy – Low Protein (HELP, 2800 Kcal/Kg ME/ 14%CP), High Energy – High Protein (HEHP, 2800 Kcal/Kg ME/ 18% CP) and Low Energy – Low Protein (LEHP, 2400 Kcal/Kg ME/ 18%CP) groups. A total of twelve FUNAAB – Alpha cocks were used for this experiment. The cocks were reared in floor pens under natural mating. At 69 weeks of age, all the cocks were slaughtered and testicles carefully removed for gross morphology and histological studies. A significant (P<0.05) influence of diet was noted on live weight with cocks fed HELP diet being heavier (P<0.05) than those in the other treatment groups. Means for all parameters of testicles measured where not different (P>0.05) from each other. Histological sections of the testes showed that HELP diet had mild effect on spermatogenesis evidenced by the scanty spermatozoa in the tubular lumen. It was concluded that FUNAAB – alpha cocks can be fed diets meant for breeding hens without adverse effect on spermatogenesis. However, feeding the LEHP diet produced roosters that were overweighed with slightly impaired spermatogenesis.


Author(s):  
Said Bilginturan

Background: In this study, the effects of different protein and energy levels in mixed feed on growth performance and reproduction characteristics of heifers were investigated. Method: The material of the study consisted of 36 Holstein Friesian (black pied) breed cattle at the age of 6 months and average live body weight of 156.65 kg and the experiment was continued for 8 months. During the experiment, four different feeds containing 16% CP - 2500 kcal / kg ME for the 1st group, 16% CP - 2700 kcal / kg ME for the 2nd group, 18% CP - 2500 kcal / kg ME for the 3rd group, 18% HP - 2700 kcal / kg ME for the 4th group were given at 1.5% of the live weight of heifers. (Where CP and ME stand for Crude Protein and Metabolizable Energy, respectively). As roughage, an equal amount of straw and alfalfa hay mixture was given. Result: As a result of this study, the effects of the different protein levels on live body weight and body measurements (body length-BL, withers height-WH, hip height-HH, hip width-HW, body depth-BD, chest girth-CG) were not found significant (P is greater than 0.05). Similarly, the effects of different energy levels on body weight and body measurements (P is greater than 0.05) and protein X energy interaction in terms of live body weight and size were also not found significant (P is greater than 0.05). Regarding the reproductive properties, the differences between the groups in terms of the effect of different protein and energy levels on the first estrus age were found statistically significant (P is less than 0.05). As a result of the analysis made in terms of heifers age during the first insemination, the difference was found to be statistically significant (P is less than 0.05). The effect of different protein and energy levels on the conception rate in the first insemination was found significant (P is less than 0.05). As a result, it was concluded that affordable mixed feed combinations can be used within the limits of mixed feed combinations containing 2500-2700 kcal / kg ME and 16-18% CP, which were given in the investigation; however, low energy-high protein combinations would give more positive results considering their effects on the reproductive performances and conception rates at first insemination.


1958 ◽  
Vol 50 (1) ◽  
pp. 97-101 ◽  
Author(s):  
W. Bolton

1. The efficiency of food utilization for egg production by pullets has been determined for two diets, one high in energy and the other low.2. Both groups laid at the same rate (245 eggs/bird) throughout the year.3. Birds fed the low-energy diet ate more food, but the intakes of digestible protein, non-protein digestible energy and metabolizable energy were the same for both groups.4. The cost of food perton was higher for the high-energy diet; per bird per year it was the same for both groups.


Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 39 ◽  
Author(s):  
Chao Yang ◽  
Jianbo Zhang ◽  
Anum Ahmad ◽  
Pengjia Bao ◽  
Xian Guo ◽  
...  

The objective of this study was to investigate the effects of different dietary energy levels on serum concentrations of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), as well as gene expression of their associated binding proteins and receptors in yak. Fifteen adult male yaks with BW of 276.1 ± 3.5 kg were allotted in three dietary groups and were fed with low (LE), medium (ME), and high energy (HE) level diet having different NEg of 5.5 MJ/kg, 6.2 MJ/kg, 6.9 MJ/kg, respectively. The effects of these treatments on ADG, BW, ADFI, and feed conversion ratio were significant (p < 0.05) throughout the experimental period. Serum GH concentration decreased (p < 0.05) with an increase in dietary energy level on d 30 and d 60. While IGF-1 concentration was higher (p < 0.05) in ME group, as compared to LE and HE groups on d 60. The expression level of growth hormone receptor (GHR) was decreased (p < 0.001) and IGF-1 was increased with the increase in the dietary energy level. The relative expression of insulin-like growth factor binding protein 3 (IGFBP-3) was higher (p < 0.001) in ME and HE groups, except the LE group. In conclusion, our findings provide a first insight into the combined effect of GH and IGF-1 in controlling the metabolism and productivity of yak. It also showed that medium energy level diet contributed to promote growth performance of yak during the cold season.


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