The effect of inoculating the bovine teat duct with small numbers of Staphylococcus aureus

1965 ◽  
Vol 32 (2) ◽  
pp. 171-179 ◽  
Author(s):  
F. H. S. Newbould ◽  
F. K. Neave
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Post Inoculation ◽  
Intramammary Infection ◽  
Colony Forming Units ◽  
Previous Infection ◽  
Glass Rods

SummaryFifty-seven inoculations of a strain of Staphylococcus aureus were made into the outer 4 mm of the teat ducts of 8 cows. The inocula ranged from 10 to 600 colony-forming units (CFU) and were made with a stainless steel instrument designed to overcome the disadvantages of glass rods and cotton swabs hitherto used.A single inoculation resulted in either no colonization of the duct, in temporary colonization for up to 7 days, or in colonization followed by intramammary infection.The recovery of the organisms depended on the size of the inoculum. When 70–100 CFU were placed in the teat duct no organisms were recovered from 12 of 24 quarters after the 1st post-inoculation milking. When 500–600 CFU were used, organisms were recovered from all of 31 quarters for at least 3 milkings, and from 61% for 6 milkings or more, in spite of dipping the teats in a strong disinfectant twice daily.Intramammary infection developed in 1 of 12 quarters (8%) inoculated in the teat duct with about 600 CFU when the animals were milked twice daily, and in 5 of 19 (23%) quarters if the 1st post-inoculation milking was omitted.There was no evidence of sensitization resulting from previous infection.

Enzyme-Linked Immunosorbent Assay and Microbiologic Culture for Diagnosis of Staphylococcus aureus Intramammary Infection in Cows

1996 ◽  
Vol 59 (1) ◽  
pp. 6-10 ◽  
Author(s):  
PAUL C. BARTLETT ◽  
RONALD J. ERSKINE ◽  
PATRICK GASTON ◽  
PHILIP M. SEARS ◽  
HENDRICUS WILHELMUS HOUDIJK
Keyword(s):  
Staphylococcus Aureus ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Relative Sensitivity ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Status ◽  
Intramammary Infection ◽  
Previous Infection ◽  
Current Infection

Recent reports have indicated that the relative sensitivity and specificity of the ELISA test for detection of intramammary infection of cows with Staphylococcus aureus is not as high as originally reported. It has been suggested that antibodies measured by enzyme-linked immunosorbent assay (ELISA) more closely reflect previous infection status rather than current infection status, and that the delay in antibody formation following infection and the persistence of antibodies after elimination of infection may be responsible for some of the discrepancy observed between ELISA and bacterial culture results conducted on the same milk sample. This study (n = 209 cows) was undertaken to determine if an ELISA for S. aureus intramammary infection more closely reflects previous infection status than it does current infection status, and to ascertain whether correction of this time-delay factor substantially improves calculated values of ELISA relative sensitivity and specificity. Receiver-operator curves were constructed to compare different time-related definitions of microbiologic culture results used for comparison with ELISA results. A greater degree of curvature in receiver-operator curves indicated that ELISA results did more closely reflect culture results performed on milk samples taken 1 and 3 weeks previously. Insignificant improvement in sensitivity and specificity occurred when the database was limited to cows (n = 140) with milk production greater than 13.6 kg/day. However, values of sensitivity were all less than or equal to 90%, and values of specificity were all less than 54%.

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from <3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

scholarly journals Heat transfer analysis of Staphylococcus aureus on stainless steel with microwave radiation

1999 ◽  
Vol 87 (3) ◽  
pp. 396-401 ◽  
Author(s):  
C. B. A. Yeo ◽  
I. A. Watson ◽  
D. E. S. Stewart-Tull ◽  
V. H. H. Koh
Keyword(s):  
Heat Transfer ◽  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Microwave Radiation ◽  
Heat Transfer Analysis

A Survey of Coliforms and Staphylococcus aureus in Cheese Using Impedimetric and Plate Count Methods1

1988 ◽  
Vol 51 (1) ◽  
pp. 53-55 ◽  
Author(s):  
F. A. KHAYAT ◽  
J. C. BRUHN ◽  
G. H. RICHARDSON
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Correlation Coefficient ◽  
Cheddar Cheese ◽  
Plate Count ◽  
Impedance Method ◽  
Colony Forming Units ◽  
Fresh Cheese ◽  
Cheese Products ◽  
And Staphylococcus Aureus

A total of 256 cheese samples were analyzed for coliform plate count using violet red bile agar and for an impedance count using BactometerR Coliform Medium with a correlation coefficient between methods of R=−.91. Fifty-four percent of the samples contained 102 to 107 colony forming units/gram (CFU/g). The highest counts were in cream and fresh cheese products. When 27 Cheddar cheese samples were inoculated with from 102 to 107 CFU of Escherichia coli/g a correlation of R=−.97 was found between methods. Two hundred of the cheese samples were analyzed for Staphylococcus aureus using Baird-Parker medium and impedance count using BactometerR S.aureus Medium. Five samples (2%) contained over 103 CFU/g. The strains isolated were coagulase-positive. When 34 samples of cheese were inoculated with 102 to 107 CFU of staphylococci/g, the correlation between the plate and impedance method was R=0.98.

Antimicrobial activity of free and liposome-encapsulated thymol and carvacrol against Salmonella and Staphylococcus aureus adhered to stainless steel

2017 ◽  
Vol 252 ◽  
pp. 18-23 ◽  
Author(s):  
Juliana Both Engel ◽  
Caroline Heckler ◽  
Eduardo Cesar Tondo ◽  
Daniel Joner Daroit ◽  
Patrícia da Silva Malheiros
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Stainless Steel ◽  
And Staphylococcus Aureus

scholarly journals Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers

2007 ◽  
Vol 38 (3) ◽  
pp. 538-543 ◽  
Author(s):  
Simone Cristina Marques ◽  
Jaíne das Graças Oliveira Silva Rezende ◽  
Lizandra Aparecida de Freitas Alves ◽  
Belami Cássia Silva ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Glass Surfaces

scholarly journals Multidrug-resistant Staphylococcus aureus (MDRSA) properties and their adherence ability on stainless steel surfaces at different temperature and time

Food Research ◽  
2020 ◽  
Vol 4 (S2) ◽  
pp. 57-66
Author(s):  
Nur-Syifa' J. ◽  
Nor-Khaizura M.A.R. ◽  
N.A. Mahyudin ◽  
Shafiqa-Atikah M.K. ◽  
Ummul-Izzatul Y.
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
High Capacity ◽  
Food Service ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Optimum Growth Temperature ◽  
Food Contact Surfaces ◽  
Steel Disc ◽  
Adherence Ability

Staphylococcus aureus is a pathogenic bacterium that capable to adhere on the processing surfaces that could cause a cross-contamination of foods. In Malaysia, S. aureus has been reported from foods and food-handlers hand at food service environment but the multidrug -resistant S. aureus (MDRSA) and their adherence on stainless steel were limited. This study was intended 1) to isolate S. aureus from food contact surfaces and characterize the isolates for MDRSA properties, and 2) to determine the adherence ability of the MDRSA strains. A total of thirty-eight S. aureus isolated from food premises in Sri Serdang were tested for the antibiotic resistance and it was carried out using five classes of antibiotics; Penicillin (I), Cephalosporins (II), Amino-glycosides (III), Quinolones Fluoroquinolone (IV), and Sulphonamide (V) by the standard procedures of Kirby-Bauer disc diffusion method. The adherence assay was performed on stainless steel disc at 25oC and 37oC on 24, 48 and 72 hrs incubation. As a result, twenty-three S. aureus were found as multidrugresistant towards the antibiotics. All the MDRSA can adhere on stainless steel with a minimum 4.00 log CFU/mL. The adherence of MDRSA on stainless steel during 72 hrs were ranging from 4.11 to 6.55 log CFU/mL and 4.25 to 6.86 log CFU/mL at 25oC and 37oC, respectively. The highest adherence was found on 48 hrs at both temperatures. The MDRSA strains revealed high capacity to adhere on stainless steel at 37oC. As a conclusion, the MDRSA strains shows the strong adherence ability at their optimum growth temperature.

Sign in / Sign up

Export Citation Format

Share Document