Detection and identification of Lactobacillus delbrueckii subsp. lactis bacteriophages by PCR

2006 ◽  
Vol 73 (2) ◽  
pp. 146-153 ◽  
Author(s):  
Miriam Zago ◽  
Angela De Lorentiis ◽  
Domenico Carminati ◽  
Lucia Comaschi ◽  
Giorgio Giraffa

A sensitive PCR method amplifying an internal fragment of the major tail protein gene was developed to detect Lactobacillus delbrueckii subsp. lactis lytic bacteriophages in undefined, thermophilic whey starters used in Italy for production of Grana and Provolone cheeses. PCR was applied to several lytic Lb. delbrueckii subsp. lactis bacteriophages, which were highly diverse according to restriction analysis and phage host range. PCR detected the presence of phages in two out of 11 cultures, when applied to whey starters for Grana Padano cheese sampled from different cheese plants. The presence of actively growing phages in infected cultures was confirmed by traditional test. The PCR method proved to be useful to screen for the presence of Lb. delbrueckii subsp. lactis phages in thermophilic whey starters.

2002 ◽  
Vol 22 (1-2) ◽  
pp. 143-149 ◽  
Author(s):  
Sandrine Petry ◽  
Satanislav Dusko Ehrlich ◽  
Emmanuelle Maguin

2021 ◽  
Vol 9 (5) ◽  
pp. 921
Author(s):  
Yoshihito Suda ◽  
Nana Sasaki ◽  
Kyoma Kagawa ◽  
Mariano Elean ◽  
Binghui Zhou ◽  
...  

Lactobacillus delbrueckii subsp. delbrueckii TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates. These two immunological and biotechnological characteristics prompted us to evaluate whether the soymilk by-product okara fermented with the TUA4408L strain can serve as an immunobiotic feed with the ability to beneficially modulate the intestinal immunity of piglets after weaning to improve their productivity. Our in vivo studies demonstrated that the administration of immunobiotic TUA4408L-fermented okara feed significantly increased piglet growth performance and meat quality. These positive effects were associated with the ability of the TUA4408L-fermented okara feed to beneficially modulate both intestinal microbiota and immunity in pigs. The immunobiotic feed improved the abundance of the beneficial bacteria Lactobacillus and Lactococcus in the gut of pigs, reduced blood markers of inflammation, and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These findings indicate that the immunobiotic TUA4408L-fermented okara feed could be an economical and environmentally friendly option to improve the growth performance and immune health of pigs.


Author(s):  
Fabienne Morel ◽  
Christophe Gilbert ◽  
Christophe Geourjon ◽  
Jacques Frot-Coutaz ◽  
Raymond Portalier ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document