Immunogenic activity of the fish tapewormPterobothrium heteracanthum(Trypanorhyncha: Pterobothriidae) in BALB/c mice

2013 ◽  
Vol 89 (2) ◽  
pp. 203-207 ◽  
Author(s):  
D.P.B.G. Mattos ◽  
M.A. Verícimo ◽  
L.M.S. Lopes ◽  
S.C. São Clemente
Keyword(s):  
Molecular Weight ◽  
Crude Protein ◽  
Specific Ige ◽  
Protein Extract ◽  
Immunoblot Assay ◽  
Crude Protein Extract ◽  
First Report ◽  
Specific Igg ◽  
Micropogonias Furnieri ◽  
Ige Response

AbstractThe aim of this study was to verify the immunogenicity ofPterobothrium heteracanthum(Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained fromMicropogonias furnieri(Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.

scholarly journals Specific IgE response in patients with brucellosis

1990 ◽  
Vol 105 (3) ◽  
pp. 571-577 ◽  
Author(s):  
G. F. Araj ◽  
A. R. Lulu ◽  
M. I. Khateeb ◽  
M. Haj
Keyword(s):  
Immunosorbent Assay ◽  
Specific Ige ◽  
Blood Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serological Markers ◽  
Microagglutination Test ◽  
Specific Igg ◽  
Ige Response

SUMMARYIn the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1–4demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81 % of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1and IgG3types while in chronic brucellosis IgG, IgA, IgE and IgG4were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

scholarly journals Total crude protein extract of Trichoderma spp. induces systemic resistance in pearl millet against the downy mildew pathogen

3 Biotech ◽  
2017 ◽  
Vol 7 (3) ◽  
Author(s):  
Boregowda Nandini ◽  
Puttaswamy Hariprasad ◽  
Harohalli Nanjegowda Shankara ◽  
Harischandra Sripathy Prakash ◽  
Nagaraja Geetha
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Crude Protein ◽  
Systemic Resistance ◽  
Protein Extract ◽  
Trichoderma Spp ◽  
Crude Protein Extract ◽  
Total Crude Protein

scholarly journals Evaluation of Antioxidant and Antidiabetic Potential of Crude Protein Extract of Pumpkin Seeds (Cucurbita maxima L.)

2020 ◽  
Vol 9 (2) ◽  
pp. 152-157
Author(s):  
Sarah Jane Monica ◽  
Sheila John ◽  
Saraswathi K ◽  
Madhanagopal R ◽  
Arumugam P
Keyword(s):  
Crude Protein ◽  
Cucurbita Maxima ◽  
Protein Extract ◽  
Crude Protein Extract ◽  
Pumpkin Seeds

scholarly journals New-vista in fi nding antioxidant and anti-infl ammatory property of crude protein extract from Sauropus androgynus leaf

2014 ◽  
Vol 13 (4) ◽  
pp. 375-383 ◽  
Author(s):  
Chakkere Shivamadhu Madhu ◽  
◽  
Honnayakanahalli Marichenne Gowda Manukumar ◽  
Puttalingaiah Basavaraju ◽  
◽  
...  
Keyword(s):  
Crude Protein ◽  
Protein Extract ◽  
Crude Protein Extract

Naturally Occurring IgG Autoantibodies to Platelet Cytoskeleton Tropomyosin

1996 ◽  
Vol 75 (04) ◽  
pp. 642-647 ◽  
Author(s):  
Ming Hou ◽  
Dick Stockelberg ◽  
Jack Kutti ◽  
Hans Wadenvik
Keyword(s):  
Molecular Weight ◽  
Human Platelet ◽  
Chronic Idiopathic Thrombocytopenic Purpura ◽  
Thrombocytopenic Purpura ◽  
Immunoblot Assay ◽  
Specific Antibodies ◽  
Platelet Protein ◽  
Naturally Occurring ◽  
Normal Individuals ◽  
Cytoskeleton Protein

SummaryWe have observed that naturally occurring serum antibodies generated a 30 Kd band in a platelet immunoblot assay. The target protein had the same molecular weight (30 Kd) under nonreduced and reduced electrophoretic conditions, and could be immunoblotted from either autologous or homologous platelet lysates. Also, the 30 Kd reactive autoantibodies could be totally adsorbed by platelet cytoskeletons. From these data one likely candidate for the autoantibody target was the intracellular platelet protein tropomyosin. Indeed, a commercially available monoclonal antitropomyosin antibody reacted with proteins comigrating with this 30 Kd band; affinity purified human platelet tropomyosin was bound by the antibodies that recognized the 30 Kd protein. This body of evidence conclusively demonstrated that naturally occurring serum autoantibodies reacted with the platelet cytoskeleton protein - tropomyosin. These tropomyosin specific antibodies were found in roughly the same percentage of sera from patients with chronic idiopathic thrombocytopenic purpura (ITP) as from normal individuals.

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