New-vista in fi nding antioxidant and anti-infl ammatory property of crude protein extract from Sauropus androgynus leaf

AbstractThe aim of this study was to verify the immunogenicity ofPterobothrium heteracanthum(Cestoda: Trypanorhyncha) crude protein extract (PH-CPE) in BALB/c mice. The parasites were obtained fromMicropogonias furnieri(Osteichthyes: Sciaenidae). Groups of six mice were each immunized with 10, 50 or 100 μg of PH-CPE, on days 0 and 35. Both specific IgG and IgE responses were developed after immunization. The immunoblot assay revealed that specific IgG recognizes PH-CPE proteins with two molecular weight ranges, 60–75 and 30–40 kDa, and that IgE recognizes larger proteins over 120 kDa. This appears to be the first report on the immunogenicity of metacestodes within the Pterobothriidae and that PH-CPE is a potential inducer of a specific IgE response.

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Toxicological evaluation and antibacterial activity of crude protein extract from endophytic bacteria associated with Algae Eucheuma spinosum

Journal of Physics Conference Series ◽

10.1088/1742-6596/1341/3/032006 ◽

2019 ◽

Vol 1341 ◽

pp. 032006

Author(s):

A Sugrani ◽

A Ahmad ◽

M N Djide ◽

H Natsir

Keyword(s):

Antibacterial Activity ◽

Endophytic Bacteria ◽

Crude Protein ◽

Protein Extract ◽

Toxicological Evaluation ◽

Crude Protein Extract

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Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract

Methods in Molecular Biology - Plant Germline Development ◽

10.1007/978-1-4939-7286-9_21 ◽

2017 ◽

pp. 265-274 ◽

Cited By ~ 2

Author(s):

Jan Fíla ◽

David Honys

Keyword(s):

Crude Protein ◽

Mature Pollen ◽

Protein Extract ◽

Crude Protein Extract ◽

Phosphoprotein Enrichment

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Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains

Dose-Response ◽

10.1177/1559325821992239 ◽

2021 ◽

Vol 19 (1) ◽

pp. 155932582199223

Author(s):

Ume Habiba ◽

Jaweria Nisar ◽

Muhammad Akram Choohan ◽

Syed Muhammad Ali Shah ◽

Zonaira Nisar ◽

...

Keyword(s):

Bacillus Subtilis ◽

Bacillus Cereus ◽

Crude Protein ◽

Protein Extraction ◽

Saccharum Officinarum ◽

Diffusion Method ◽

Albizia Lebbeck ◽

Zone Of Inhibition ◽

Cassia Fistula ◽

Protein Extract

Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against Escherichia coli, Bacillus subtilis, Neisseria gonorrhoea, Bacillus cereus and Proteus mirabilis. Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts’ antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of Cymbopogon citrates showed maximum zone of inhibition 11 mm and 9 mm against Bacillus subtilis and Bacillus cereus respectively and control chloramphenicol showed maximum zone of inhibition 26 mm against Bacillus subtilis. Cassia fistula showed maximum zone of inhibition of 7 mm against Bacillus cereus while Saccharum officinarum and Albizia lebbeck didn’t show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn’t show zone of inhibition against any bacteria. Only Albizia lebbeck showed minute zone of inhibition against Neisseria gonorrhea. On well diffusion method, Cassia fistula Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against Proteus mirabilis and Bacillus subtilis respectively. While Albizia lebbeck PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against Bacillus subtilis and Bacillus cereus. The results revealed that the protein extract of Albizia lebbeck, Cymbopogon citrates and Cassia fistula can be used tosynthesize antimicrobial drugs to treat the bacterial infections.

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Enzymological aspects of de novo synthesis of fructooligosaccharides in leaf disks of certain Asteraceae. IV. The activity of sucrose–sucrose 1-fructosyltransferase

Canadian Journal of Botany ◽

10.1139/b74-178 ◽

1974 ◽

Vol 52 (6) ◽

pp. 1369-1377 ◽

Cited By ~ 18

Author(s):

K. R. Chandorkar ◽

F. W. Collins

Keyword(s):

Crude Protein ◽

De Novo ◽

Gel Filtration ◽

Apparent Molecular Weight ◽

Degree Of Polymerization ◽

Transferase Activity ◽

Protein Extract ◽

Sucrose Medium ◽

Filtration Technique ◽

Leaf Disks

Sucrose–sucrose fructosyltransferase capable of synthesizing the trisaccharide 1F-fructosylsucrose from sucrose was partially purified from crude protein extracts of (1) lettuce leaf disks, which were incubated on a sucrose medium for 72 h, and (2) lettuce leaf midvein–petiole tissue, which normally contains fructosans ranging in degree of polymerization from 3 to about 10–11. Maximum transferase activity was associated with the protein exhibiting an apparent molecular weight of about 100 000 as estimated by gel filtration technique. The transferase was not detected in protein extract of unincubated leaf blade tissue. Evidence presented strongly suggests that the transferase was synthesized de novo in response to incubation of leaf disks on sucrose medium.

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Effects of Increased Dietary Protein and Energy on Composition and Functional Capacities of Blood Mononuclear Cells from Vaccinated, Neonatal Calves

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.75.5.357 ◽

2005 ◽

Vol 75 (5) ◽

pp. 357-368 ◽

Cited By ~ 8

Author(s):

Foote ◽

Nonnecke† ◽

Waters ◽

Palmer ◽

Beitz ◽

...

Keyword(s):

Weight Gain ◽

B Cell ◽

Dietary Protein ◽

Crude Protein ◽

Interleukin 2 ◽

Milk Replacer ◽

Standard Diet ◽

Gamma Delta ◽

Ifn Gamma ◽

Functional Capacities

Effects of increased protein and energy provided by an intensified milk replacer on the antigen-specific, cell-mediated immune response of the neonatal calf were examined. Calves were fed a standard (0.45 kg/day of a 20% crude protein, 20% fat milk replacer; n = 11) or intensified (1.14 kg/day of a 28% crude protein, 20% fat milk replacer; n = 11) diet from 0 to 6 weeks of age. All calves were vaccinated with Mycobacterium bovis bacillus Calmette-Guerin (BCG) at 1 week of age. The daily weight gain of intensified-diet calves (0.62 kg/day) was greater than the weight gain of standard-diet calves (0.29 kg/day). Liver, kidney, heart, thymus, and subcervical lymph nodes from intensified-diet calves were heavier than the same organs from standard-diet calves. Flow cytometric analysis of peripheral blood mononuclear cell (PBMC) populations indicated that CD4+ cells, gamma delta TCR+ cells, and monocyte percentages, although unaffected by diet during the first 5 weeks of the study, were higher in intensified-diet calves at week 6. The decline in gamma deltad TCR+ cell percentages and increase in B cell percentages with increasing age seen in all calves are characteristic of the maturing immune system of the calf. CD8+ T cell or B cell percentages were not affected by diet. In intensified-diet calves, percentages of CD4+ expressing interleukin-2 receptor increased and percentages of gamma delta TCR+ cells expressing interleukin-2 receptor decreased with time. The same populations in standard-diet calves did not change with time. Percentages of CD4+ and CD8+ T cells, and B cells expressing MHC class II antigen, were unaffected by diet or age. Although mitogen-induced interferon (IFN)-gamma and nitric oxide (NO) secretion increased with age for all calves, PBMC from intensified-diet calves produced less IFN-gamma and more NO than did cells from standard-diet calves at week 6 of the study. Antigen-induced secretion of IFN-gamma and NO also increased with age but was unaffected by diet. Antigen-elicited delayed-type hypersensitivity was unaffected by diet, suggesting increased dietary protein and energy did not alter adaptive immunity in vivo. Overall, these results suggest that feeding calves a commercially available, intensified milk replacer affects minimally the composition and functional capacities of PBMC populations. Additional research is necessary to determine whether these subtle effects influence the calf’s susceptibility to infectious disease.

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