Phylogenetic relations of European shrubby taxa of the genusUsnea

2011 ◽  
Vol 43 (5) ◽  
pp. 427-444 ◽  
Author(s):  
Lauri SAAG ◽  
Tiiu TÕRRA ◽  
Andres SAAG ◽  
Ruth DEL-PRADO ◽  
Tiina RANDLANE
Keyword(s):  
Maximum Parsimony ◽  
Chemical Separation ◽  
Molecular Data ◽  
Beta Tubulin ◽  
Phylogenetic Relations ◽  
Branch Lengths ◽  
Nuclear Its ◽  
Molecular Characters

AbstractThis study focuses on EuropeanUsneaspecies with sorediate shrubby thalli, with the aim to evaluate the morphological and chemical separation of species in the light of molecular data. Twenty-twoUsneaspecies, including widely distributed taxa such asU. diplotypus, U. fulvoreagens, U. glabrescens, U. lapponica, U. subfloridana, U. substerilisandU. wasmuthii, were included in the study using Bayesian and maximum parsimony analyses of nuclear ITS and beta-tubulin sequences. The analyses showed that: 1) most taxa that are morphologically well delimited are also distinct by means of molecular characters, 2) shrubby taxa in the sectionUsneathat are difficult to determine by traditional characters form a group of closely related but still genetically distinct entities, exceptU. diplotypusandU. substeriliswhich appear to be polyphyletic. The branch lengths differed largely between two parts of the ITS tree (sectionsUsneaandCeratinae).Usnea intermediais proposed as the sexually reproducing counterpart for the sorediateU. lapponica. Additionally, some new chemotypes ofUsneaspecies were determined.

scholarly journals Contribution to Globularia phylogeny based on nuclear ribosomal spacer and two chloroplast DNA regions

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Kroata Hazler Pilepić ◽  
Maja Friščić ◽  
Ahmet Duran ◽  
Semir Maslo ◽  
Rade Garić ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Maximum Parsimony ◽  
Nuclear Dna ◽  
Sequence Data ◽  
Leaf Tissue ◽  
Molecular Data ◽  
Rbcl Gene ◽  
Nuclear Its ◽  
Cellular Dna ◽  
Chloroplast Dna Regions

Background and Purpose: Molecular approach has a major impact on phylogenetic studies of plants, considering that it gives useful information about evolutionary events and relations on all taxonomic levels. The sequence data of the nuclear ITS and of two chloroplast regions, trnL-trnF spacer and rbcL gene, obtained from thirteen Globularia L. taxa, including five Anatolian endemics, representing six sections altogether, were analyzed in order to determine the relations between the European and the Anatolian species and get a better insight into the phylogeny of several closely related Globularia taxa.  Materials and Methods: Total cellular DNA was extracted from fresh or frozen leaf tissue of thirteen Globularia samples. The ITS regions of nuclear DNA and two chloroplast DNA regions were amplified and sequenced. Obtained nuclear and combined plastid data matrices were subjected to Maximum Parsimony analyses. Results and Conclusions: Molecular data that were obtained in this study indicate the existence of separate centers of diversification for the European and the Anatolian Globularia. The results provide support for relationships among the studied Anatolian endemic species and indications for a redefinition of affinities of some of the European species. The results presented herein are discussed along with available morphological, karyological, phytogeographical and molecular data. Keywords: Globularia, ITS, trnL-trnF, rbcL, Maximum Parsimony, Quaternary

The status of Myriangiaceae (Dothideomycetes)

Phytotaxa ◽  
2014 ◽  
Vol 176 (1) ◽  
pp. 219 ◽  
Author(s):  
ASHA J. DISSANAYAKE ◽  
RUVISHIKA S. JAYAWARDENA ◽  
SARANYAPHAT BOONMEE ◽  
KASUN M. THAMBUGALA ◽  
QING TIAN ◽  
...  
Keyword(s):  
Sequence Data ◽  
Molecular Data ◽  
Molecular Sequence Data ◽  
Molecular Sequence ◽  
The Family ◽  
The Status ◽  
Sexual State ◽  
Morphological And Molecular Data ◽  
Asexual State ◽  
Molecular Characters

The family Myriangiaceae is relatively poorly known amongst the Dothideomycetes and includes genera which are saprobic, epiphytic and parasitic on the bark, leaves and branches of various plants. The family has not undergone any recent revision, however, molecular data has shown it to be a well-resolved family closely linked to Elsinoaceae in Myriangiales. Both morphological and molecular characters indicate that Elsinoaceae differs from Myriangiaceae. In Elsinoaceae, small numbers of asci form in locules in light coloured pseudostromata, which form typical scab-like blemishes on leaf or fruit surfaces. The coelomycetous, “Sphaceloma”-like asexual state of Elsinoaceae, form more frequently than the sexual state; conidiogenesis is phialidic and conidia are 1-celled and hyaline. In Myriangiaceae, locules with single asci are scattered in a superficial, coriaceous to sub-carbonaceous, black ascostromata and do not form scab-like blemishes. No asexual state is known. In this study, we revisit the family Myriangiaceae, and accept ten genera, providing descriptions and discussion on the generic types of Anhellia, Ascostratum, Butleria, Dictyocyclus, Diplotheca, Eurytheca, Hemimyriangium, Micularia, Myriangium and Zukaliopsis. The genera of Myriangiaceae are compared and contrasted. Myriangium duriaei is the type species of the family, while Diplotheca is similar and may possibly be congeneric. The placement of Anhellia in Myriangiaceae is supported by morphological and molecular data. Because of similarities with Myriangium, Ascostratum (A. insigne), Butleria (B. inaghatahani), Dictyocyclus (D. hydrangea), Eurytheca (E. trinitensis), Hemimyriangium (H. betulae), Micularia (M. merremiae) and Zukaliopsis (Z. amazonica) are placed in Myriangiaceae. Molecular sequence data from fresh collections is required to confirm the relationships and placement of the genera in this family.

Wadithamnus, a new monotypic genus in Amaranthaceae

Phytotaxa ◽  
2017 ◽  
Vol 295 (2) ◽  
pp. 173 ◽  
Author(s):  
TIMOTHY A. HAMMER ◽  
ROBERT W. DAVIS ◽  
KEVIN R. THIELE
Keyword(s):  
Molecular Data ◽  
Coastal Areas ◽  
Monotypic Genus ◽  
Distinct Genus ◽  
Nuclear Its ◽  
Basal Position

A new monotypic genus from near-coastal areas of Oman and Yemen is here described and named Wadithamnus. The generitype is Wadithamnus artemisioides comb. nov. (basionym Aerva artemisioides). On the basis of morphology, W. artemisioides can be separated from Aerva on the basis of its 3(–7)-flowered cymes and flowers with two outer and four inner tepals, and four stamens alternating with the inner tepals. Molecular data (nuclear ITS and chloroplast trnK–matK sequences) confirm Wadithamnus as a distinct genus outside Aerva, the latter marker placing it at a basal position to the achyranthoid clade. The name Aerva artemisioides is lectotypified on a specimen preserved at WU.

scholarly journals Morphological and molecular identification of a new species of Atraporiella (Polyporales, Basidiomycota) in China

Phytotaxa ◽  
2017 ◽  
Vol 332 (1) ◽  
pp. 31 ◽  
Author(s):  
ZI-QIANG WU ◽  
SHAN SHEN ◽  
KAI-YUE LUO ◽  
ZHENG-HUI WANG ◽  
CHANG-LIN ZHAO
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Molecular Data ◽  
Fungal Species ◽  
Monophyletic Lineage ◽  
Inference Methods ◽  
A New Species ◽  
Molecular Characters

A new poroid wood-inhabiting fungal species, Atraporiella yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by cream pore surface when dry, which is easy to separate from substrate and very rapidly stained dark brown to black when bruised; hyphal system monomitic with generative hyphae hyaline to pale brown, thin-walled, unbranched, interwoven; slightly allantoid basidiospores, 2.2–3 × 0.8–1.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Atraporiella yunnanensis belonged to the residual polyporoid clade, formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and was closely related to A. neotropica, and then grouped with other related genera: Antrodiella, Pouzaroporia, Steccherinum and Xanthoporus. Both morphological and molecular characters confirmed the placement of the new species in Atraporiella.

Cymbidium xichouense (Orchidaceae; Epidendroideae), a new species from China: evidence from morphological and molecular data

Phytotaxa ◽  
2021 ◽  
Vol 484 (3) ◽  
pp. 291-297
Author(s):  
XINYU XU ◽  
CHANG-CHUN DING ◽  
WENQI HU ◽  
XIA YU ◽  
YU ZHENG ◽  
...  
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Phylogenetic Analyses ◽  
Plastid Dna ◽  
Molecular Data ◽  
Morphological Evidence ◽  
The Status ◽  
Nuclear Its ◽  
Morphological And Molecular Data ◽  
A New Species

A new species of Cymbidium (Orchidaceae), Cymbidium xichouense, from Yunnan Province, China, is described and illustrated based on morphological evidence and molecular analyses. The new orchid is morphologically similar to C. qinbeiense, but it has several morphological features that distinguish it from C. qiubeinense and all other recognized species in Cymbidium. Phylogenetic analyses based on nuclear (ITS) and plastid DNA (matK) were conducted, and the results also supported the status of C. xichouense as a new species, which is sister to C. qiubeiense.

Monographic revision of the endemic Helix mazzullii De Cristofori & Jan, 1832 complex from Sicily and re-introduction of the genus Erctella Monterosato, 1894 (Pulmonata, Stylommatophora, Helicidae)

Zootaxa ◽  
2011 ◽  
Vol 3134 (1) ◽  
pp. 1 ◽  
Author(s):  
M. STELLA COLOMBA ◽  
ARMANDO GREGORINI ◽  
FABIO LIBERTO ◽  
AGATINO REITANO ◽  
SALVATORE GIGLIO ◽  
...  
Keyword(s):  
Molecular Study ◽  
Molecular Data ◽  
Comprehensive Analysis ◽  
Molecular Dating ◽  
12S Rrna ◽  
Ecological Significance ◽  
Ecological Features ◽  
As Species ◽  
Individual Segment ◽  
Nuclear Its

Helix mazzullii De Cristofori & Jan, 1832 s.l.(Pulmonata, Stylommatophora, Helicidae) is an endemic, rupicolous, saxicavous taxon of northwestern Sicily. Its populations are vulnerable and it is of great ecological significance. However, its taxonomy, phylogeny and biogeography are still uncertain. The present paper reports on a comprehensive analysis of morphological diagnostic characters (shell and genitalia) joined by the molecular study of two mitochondrial (16S rRNA and 12S rRNA) and one nuclear (ITS-2) partial gene sequences, investigated by individual segment analyses or combining the three gene fragments with a concatenate analysis. Our results corroborated the hypothesis that this species is rather a group (i.e., the mazzullii group) including three different taxa (mazzullii, cephalaeditana and insolida) recognized as species. Moreover, molecular dating of lineages suggests that this complex might have occurred long before the Messinian salinity crisis. Finally, peculiar morphological and ecological features along with molecular data strongly support the proposal to re-introduce the genus Erctella Monterosato, 1894 for the H. mazzullii complex. Synonyms and bibliographic references are reported in the systematic part; collection records are listed in Appendix 1.

Contributions to the taxonomy of Rhyncholacis (Podostemaceae): Evidence of monophyly, description of a new species, and transfer of the monotypic Macarenia

Phytotaxa ◽  
2018 ◽  
Vol 357 (2) ◽  
pp. 107 ◽  
Author(s):  
C. THOMAS PHILBRICK ◽  
BRAD R. RUHFEL ◽  
CLAUDIA P. BOVE
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Phylogenetic Study ◽  
Trnl Intron ◽  
Nuclear Its ◽  
Lobed Leaf ◽  
A New Species ◽  
Made In

We conducted a phylogenetic study of neotropical subfamily Podostemoideae with a focus on Rhyncholacis and the monotypic Macarenia using molecular data (plastid: rbcL, trnL intron; nuclear: ITS). Our results placed the five included species of Rhyncholacis, one of which is newly described herein, in a moderately well supported (73 BP) clade with M. clavigera. These results support the transfer of M. clavigera to Rhyncholacis (R. clavigera); the nomenclatural changes are made. In addition, a new species of Rhyncholacis (R. paulana C.T. Philbrick & C.P. Bove) is illustrated and described. Rhyncholacis paulana is distinguished from all other species in the genus by its simple pinnately lobed leaf, which is fleshy and undulate. All other species of Rhyncholacis have leaves that are pinnately lobed, the lobes of which are repeatedly divided, or pinnately compound and characterized by finely dissected pinnate segments.

scholarly journals Molecular phylogeny of the Taeniapterini (Diptera: Micropezidae) using nuclear and mitochondrial DNA, with a reclassification of the genus Taeniaptera Macquart

2015 ◽  
Vol 46 (5) ◽  
pp. 411-430 ◽  
Author(s):  
Morgan D. Jackson ◽  
Stephen A. Marshall ◽  
Jeffrey H. Skevington
Keyword(s):  
Mitochondrial Dna ◽  
Bayesian Inference ◽  
Molecular Phylogeny ◽  
Maximum Parsimony ◽  
New World ◽  
Molecular Data ◽  
Species Group ◽  
Mitochondrial Genes ◽  
Paraphyletic Group ◽  
Total Dna

DNA molecular data are used to generate a phylogeny for the micropezid subfamily Taeniapterinae. Thirty-two taeniapterine species were sampled, including 10 of the 20 New World genera recognized by Steyskal, as well as one genus formerly treated as a synonym of Poecilotylus Hennig (Hemichaeta Steyskal). Five species from the Micropezinae were included as outgroups. A total DNA dataset of 4705 bp, including mitochondrial genes (12S and cytochrome c oxidase I (COI)) and nuclear coding genes (wingless and CAD), was analysed using maximum parsimony and Bayesian inference. The genus Taeniaptera Macquart was found to be non-monophyletic with respect to the remainder of the Taeniapterini analysed here. Taeniaptera is restricted to the Taeniaptera trivittata Macquart species group, Mitromyia Cresson is resurrected to contain the Taeniaptera grata (Wulp) species group, and Paragrallomyia Hendel is resurrected to contain most species previously considered Taeniaptera. Poecilotylus is recognized as a paraphyletic group awaiting further research.

Biatora Britannica sp. Nov. and the Occurrence of Biatora Efflorescens in the British Isles

2001 ◽  
Vol 33 (3) ◽  
pp. 181-187 ◽  
Author(s):  
Christian Printzen ◽  
H. Thorsten Lumbsch ◽  
Alan Orange
Keyword(s):  
Its Region ◽  
Molecular Data ◽  
Sister Taxon ◽  
British Isles ◽  
Close Relationship ◽  
Nuclear Its ◽  
Relationship Of

AbstractBiatora Britannica sp. nov is described from Wales. The species is the sorediate sister taxon of the Madeiran Biatora hertelii. The close relationship is supported by the presence of the hymenial pigment Hertelii-green, which is hitherto known only from these two species. Molecular data from the nuclear ITS-region of 14 Bratora¯ species renders further support for the close relationship of both species. Sterile, sorediate material from the British Isles earlier referred to B. efflorescens may in fact belong to B. britannica.

Dunnarts from Boullanger Island: new evidence and reassessment of a taxonomic issue with resource implications.

2006 ◽  
Vol 28 (1) ◽  
pp. 51 ◽  
Author(s):  
A. N. Start ◽  
D. Moro ◽  
M. Adams ◽  
R. Bencini
Keyword(s):  
Genetic Study ◽  
Molecular Data ◽  
Morphological Data ◽  
Island Population ◽  
New Taxon ◽  
Fresh Tissue ◽  
New Subspecies ◽  
New Evidence ◽  
Molecular Characters

Crowther et al. (1999) described an island population of an Australian dasyurid marsupial, Sminthopsis griseoventer, as a new subspecies on molecular (allozyme) and morphological grounds despite a previously published, contradictory genetic study and evidence of sympatry with its mainland conspecific. The legitimacy of this taxonomic arrangement has implications for the allocation of scarce management resources because the new taxon could be considered 'Critically Endangered'. Samples of the original tissues from which the molecular data cited by Crowther et al. were obtained no longer exist. Therefore, salient components of that work were rerun with fresh tissue and the results used to reassess evidence that the island population warrants recognition as a subspecies. We conclude that neither the molecular nor the morphological data supports differentiation at taxonomic or evolutionarily significant levels. Nevertheless, there is a good case for recognising the island population as an important management unit. This case study emphasises the importance of sound taxonomy determined by macro and molecular characters as a prerequisite to allocating resources for conservation.

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