A new leprose Leprocaulon (Ascomycota, Leprocaulales) from Great Britain

2017 ◽  
Vol 49 (3) ◽  
pp. 183-188 ◽  
Author(s):  
Alan ORANGE ◽  
Peter M. EARLAND-BENNETT ◽  
Christopher J. B. HITCH ◽  
Mark POWELL
Keyword(s):  
Fatty Acids ◽  
Great Britain ◽  
North America ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Usnic Acid ◽  
Its Sequence ◽  
Chemical Studies ◽  
Calcareous Rock ◽  
Layer Chromatography

AbstractLeprocaulon calcicola is described as new from walls in SE England; it is leprose, pale to mid blue-grey, and contains zeorin and usnic acid. It differs from L. knudsenii from North America in its habitat on mortared walls rather than non-calcareous rock and in its ITS sequence. ‘Lecanora’ ecorticata differs in the yellower colour, and the presence of unidentified fatty acids and traces of unknown terpenoids (but not zeorin) by thin-layer chromatography. Leprose lichens with usnic acid are still poorly known and sequencing must be used to support morphological and chemical studies.

scholarly journals Chemical Studies on Lichens. 5. Separation and Identification of the Antipodes of Usnic Acid by Thin Layer Chromatography.

1967 ◽  
Vol 21 ◽  
pp. 1376-1377 ◽  
Author(s):  
Gerd Bendz ◽  
Gerd Bohman ◽  
Johan Santesson ◽  
Pär Holmberg ◽  
G. Eriksson ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Usnic Acid ◽  
Chemical Studies ◽  
Layer Chromatography

Diacylglycerol Biosynthesis in Everted Sacs of Rat Intestinal Mucosa

1975 ◽  
Vol 53 (11) ◽  
pp. 1170-1183 ◽  
Author(s):  
W. C. Breckenridge ◽  
A. Kuksis
Keyword(s):  
Fatty Acids ◽  
Phosphatidic Acid ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Thin Layer Chromatography ◽  
Intestinal Mucosa ◽  
Gas Liquid Chromatography ◽  
Acid Pathway ◽  
Layer Chromatography ◽  
Everted Sacs

The molecular specificity in the biosynthesis of diacylglycerols by rat intestinal mucosa was examined by means of radioactive markers, thin-layer chromatography with silver nitrate and gas-liquid chromatography with radioactivity monitoring. Bile salt micelles of alternately labeled monoacylglycerols and free fatty acids were incubated with everted sacs of intestinal mucosa for various periods of time and the diacylglycerols were isolated by solvent extraction and thin-layer chromatography. Stereospecific analyses of the X-1,2-diacylglycerols labeled from 2-monoacylgiycerols showed that the sn-1,2-isomers (45–55%) were slightly in excess of the sn-2,3-isomers (34–45%) with the X-1,3-diacylglycerols accounting for the rest of the radioactivity (5–10%). This suggests that racemic diacylglycerols may be intermediates in the resynthesis of dietary fat in rat intestinal mucosa. Detailed analyses of the molecular species of the sn-1,2-diacylglycerols labeled from free fatty acids revealed that 10–45% of the total did not contain the acid present in the 2-monoacylglycerol supplied, and therefore had originated from the phosphatidic acid pathway. These findings are at variance with those obtained in isolated microsomes, which have suggested an inhibition of the phosphatidic acid pathway by monoacylglycerols as well as have given evidence of an exclusive synthesis of sn-1,2-diacylglycerols from 2-monoacylglycerols.

Standardization of Homoeopathic Mother Tincture of Strychnous nux vomica with the help of High-Performance Thin Layer Chromatography and correlation of its alkaloid markers with its Toxicological action

2021 ◽  
pp. 4203-4206
Author(s):  
Dharmendra B. Sharma ◽  
Parth Aphale ◽  
Vineet Sinnarkar ◽  
Sohan S. Chitlange ◽  
Asha Thomas
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Therapeutic Action ◽  
Aluminium Sheets ◽  
Mother Tincture ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Dark Blue

Background: Chromatography is one of the important laboratory technique in which the components of a mixture are separated on an adsorbent in order to analyze, identify, purify and quantify a mixture. Thin Layer Chromatography (TLC)is used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound. High Performance Thin Layer Chromatography is a sophisticated and automated form of Thin Layer Chromatography (TLC). The procedure simultaneously processes the sample and standard that results in better analytical precision and accuracy at a faster pace. Pharmacological/ Toxicological action of Nux Vomica is because of its active principles present in the seeds namely strychnine, brucine etc. This research paper aims to corelate the active principles present in Nux Vomica with the toxicological action of the same. Materials and Methods: 1. Standard Nux Vomica mother tincture was tested for its alkaloid markers and its correlation with the toxicological action was studied. 2. Analysis of the mother tincture was done using High Performance Thin Layer Chromatography. 3. Stationary phase consisted of TLC Aluminium sheets with silica gel 60 F253 pre-coated layer (20cm x 10cm), thickness-0.2mm, no. of tracks-18, band length-6mm. 4. Mobile Phase consisted of Chloroform: Methanol (9.5:0.5). 5. The plate was developed in developing chamber and observed under U.V. Light. Results: Colours seen on the HPTLC Plates of samples are greenwhich corresponds to strychnine, dark blue which corresponds to brucine, orange to alkaloids fluorescent green to sterols and pink to fatty acids which are evident on the chromatogram. Conclusion: Therapeutic action of Nux Vomica as noted in Homoeopathic Materia Medica is because of the active principles like strychnine, brucine, alkaloids, sterols, fatty acids present in it which is evident from the chromatogram.

scholarly journals Recovery of Bioactive Compounds from Hazelnuts and Walnuts Shells: Quantitative–Qualitative Analysis and Chromatographic Purification

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1363 ◽  
Author(s):  
René Herrera ◽  
Jarl Hemming ◽  
Annika Smeds ◽  
Oihana Gordobil ◽  
Stefan Willför ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Antioxidant Capacity ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Short Chain Fatty Acids ◽  
Extraction Process ◽  
Size Exclusion ◽  
Flash Chromatography ◽  
Layer Chromatography

Hazelnut (HS) and walnut (WS) shells, an abundant by-product of the processing industries of these edible nuts, are traditionally considered as a low-value waste. However, they are a source of valuable compounds with an interesting chemical profile for the chemical and pharmaceutical sectors. In this study, the lipophilic and hydrophilic extracts present in HS and WS were quantified and identified, then the polar fractions were chromatographically separated, and their antioxidant capacity was studied. The experimental work includes the isolation of crude lipophilic and hydrophilic extracts by an accelerated extraction process, chromatographic analysis (gas chromatography-flame ionization (GC-FID), GC-mass spectroscopy (GC-MS), high-performance size-exclusion chromatography (HPSEC), thin-layer chromatography (TLC)), and quantification of the components. In addition, a thorough compositional characterization of the subgroups obtained by flash chromatography and their antioxidant capacity was carried out. The gravimetric concentrations showed different lipophilic/hydrophilic ratios (0.70 for HS and 0.23 for WS), indicating a higher proportion of polar compounds in WS than in HS. Moreover, the lipophilic extracts were principally composed of short-chain fatty acids (stearic, palmitic, and oleic acid), triglycerides, and sterols. The polar fractions were screened by thin-layer chromatography and then separated by flash chromatography, obtaining fractions free of fatty acids and sugar derivatives (97:3 in HS and 95:5 in WS), and mixtures richer in phenolic compounds and flavonoids such as guaiacyl derivatives, quercetin, pinobanksin, and catechin. The most polar fractions presented a higher antioxidant capacity than that of the crude extracts.

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