High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

AbstractHigh performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.

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High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

Journal of Pharmaceutical and Biological Sciences ◽

10.18231/j.jpbs.2021.002 ◽

2021 ◽

Vol 9 (1) ◽

pp. 7-14

Author(s):

R B Pandhare ◽

R N Kulkarni ◽

V K Deshmukh ◽

P B Mohite ◽

A R Pawar

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Herbal Drugs ◽

Biomedical Analysis ◽

Analytical Technique ◽

Short Period ◽

Lichen Substances ◽

Medicinal Drugs ◽

Layer Chromatography

HPTLC is an advanced type of planar chromatography used extensively in the recent years for fingerprinting of medicinal plants, products and for screening lichen substances, quantification of active ingredients and herbal drugs, phytochemical and biomedical analysis and also used to check of presence of adulterants in the formulation. It is more sensitive and possible to run more sample in a short period of time, by using small amount of solvent. It is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. In this paper, a brief discussion of the type of instrument used in HPTLC, its entire approach, and how this procedure is superior to TLC is presented. This article attempts to concentrate on the use of HPTLC by including examples of medications, medicinal drugs, and formulations that have been analyzed using HPTLC.

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The occurrence of biomineralization products in four lichen species growing on sandstone in western Norway

The Lichenologist ◽

10.1006/lich.2002.0413 ◽

2002 ◽

Vol 34 (5) ◽

pp. 429-440 ◽

Cited By ~ 30

Author(s):

Torbjørg Bjelland ◽

Linda Sæbø ◽

Ingunn H. Thorseth

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Sem Analysis ◽

X Ray Diffraction ◽

Western Norway ◽

Lichen Substances ◽

Electron Microscopy Analysis ◽

Microscopy Analysis ◽

Layer Chromatography

AbstractHigh performance thin-layer chromatography/thin-layer chromatography, X-ray diffraction, and scanning electron microscopy analysis of thallus and lichen-rock interface samples, were undertaken to characterize biomineralization products in Fuscidea cyathoides, Ochrolechia tartarea, Ophioparma ventosa, and Pertusaria coralline, growing on sandstone in western Norway. Whewellite (monohydrate form of Ca oxalate) was found in the thallus of all species, but not in any of the weathering rinds beneath the species. A significantly higher amount of whewellite was detected in the thalli of F. cyathoides and O. ventosa than in the other two species. There were only a few differences in whewellite occurrence between the thallus edge and centre samples in the four species. HPTLC/TLC and SEM analysis indicate that lichen compounds occur within the rock beneath some of the lichen specimens. Only divaricatic acid was observed within the weathering rind beneath O. ventosa. No lichen substances were found in the weathering rind beneath F. cyathoides and P. corallina, whereas gyrophoric and lecanoric acids were found in the weathering rind beneath O. tartarea.

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