scholarly journals High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery

2021 ◽  
Vol 9 (1) ◽  
pp. 7-14
Author(s):  
R B Pandhare ◽  
R N Kulkarni ◽  
V K Deshmukh ◽  
P B Mohite ◽  
A R Pawar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Herbal Drugs ◽  
Biomedical Analysis ◽  
Analytical Technique ◽  
Short Period ◽  
Lichen Substances ◽  
Medicinal Drugs ◽  
Layer Chromatography

HPTLC is an advanced type of planar chromatography used extensively in the recent years for fingerprinting of medicinal plants, products and for screening lichen substances, quantification of active ingredients and herbal drugs, phytochemical and biomedical analysis and also used to check of presence of adulterants in the formulation. It is more sensitive and possible to run more sample in a short period of time, by using small amount of solvent. It is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. In this paper, a brief discussion of the type of instrument used in HPTLC, its entire approach, and how this procedure is superior to TLC is presented. This article attempts to concentrate on the use of HPTLC by including examples of medications, medicinal drugs, and formulations that have been analyzed using HPTLC.

High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

1993 ◽  
Vol 25 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Ulf Arup ◽  
Stefan Ekman ◽  
Louise Lindblom ◽  
Jan-Eric Mattsson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Lichen Substances ◽  
Improved Technique ◽  
Layer Chromatography

AbstractHigh performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.

scholarly journals The occurrence of biomineralization products in four lichen species growing on sandstone in western Norway

2002 ◽  
Vol 34 (5) ◽  
pp. 429-440 ◽  
Author(s):  
Torbjørg Bjelland ◽  
Linda Sæbø ◽  
Ingunn H. Thorseth
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Western Norway ◽  
Lichen Substances ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Layer Chromatography

AbstractHigh performance thin-layer chromatography/thin-layer chromatography, X-ray diffraction, and scanning electron microscopy analysis of thallus and lichen-rock interface samples, were undertaken to characterize biomineralization products in Fuscidea cyathoides, Ochrolechia tartarea, Ophioparma ventosa, and Pertusaria coralline, growing on sandstone in western Norway. Whewellite (monohydrate form of Ca oxalate) was found in the thallus of all species, but not in any of the weathering rinds beneath the species. A significantly higher amount of whewellite was detected in the thalli of F. cyathoides and O. ventosa than in the other two species. There were only a few differences in whewellite occurrence between the thallus edge and centre samples in the four species. HPTLC/TLC and SEM analysis indicate that lichen compounds occur within the rock beneath some of the lichen specimens. Only divaricatic acid was observed within the weathering rind beneath O. ventosa. No lichen substances were found in the weathering rind beneath F. cyathoides and P. corallina, whereas gyrophoric and lecanoric acids were found in the weathering rind beneath O. tartarea.

High Performance Thin Layer Chromatography: A Mini Review

2016 ◽  
Vol 2 (4) ◽  
pp. 219-226
Author(s):  
S. V. Saibaba ◽  
P. Shanmuga Pandiyan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Separation Efficiency ◽  
Analytical Tool ◽  
Combinatorial Approach ◽  
Herbal Drug ◽  
Biomedical Analysis ◽  
Layer Chromatography

High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have made HPTLC a power analytical tool in the field of analysis. Experts are of the opinion that HPTLC future to combinatorial approach and the utilization of instrumental HPTLC toward the analysis of drug formulations, bulk drugs, and natural products will increase in the future

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