Antigenic similarity among culture forms of the ‘brucei’ group of trypanosomes

Parasitology ◽  
1964 ◽  
Vol 54 (3) ◽  
pp. 593-596 ◽  
Author(s):  
J. Richard Seed
Keyword(s):  
Specific Antiserum ◽  
Agglutination Reaction ◽  
Antigenic Similarity ◽  
Cross Reactions ◽  
Cultural Forms

Extensive cross-reactions have been found between cultural forms of trypanosomes of the ‘brucei’ group by the agglutination reaction. The antigen (or antigens) responsible for agglutination are apparently specific to the ‘brucei’ group of trypanosomes. These antigens are also quite stable to the presence of specific antiserum in contrast to the serotype of the blood trypanosomes which are known to be altered in the presence of specific antiserum.

scholarly journals Characterization of epitope specificity of Proteus penneri 7 lipopolysaccharide core region.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Agata Palusiak ◽  
Zygmunt Sidorczyk
Keyword(s):  
Core Region ◽  
Specific Antiserum ◽  
Specific Antibodies ◽  
Cross Reactions ◽  
Epitope Specificity ◽  
The Core ◽  
Serological Studies ◽  
The Cross ◽  
Proteus Penneri

To extend the knowledge on the fragments of Proteus penneri lipopolysaccharide core regions, which determine the cross-reactions with specific antibodies, serological studies were performed by use of P. penneri 7 core-specific antiserum and Proteus sp. lipopolysaccharides. Different reactivity of the tested antiserum with three groups of antigens suggested differences in their core regions' epitope specificity. Comparing the results of the serological investigations with the previously determined structures of the core regions of the tested P. penneri lipopolysaccharides allowed distinguishing two potential tri- and tetrasaccharide epitopes and a third fragment which could not be determined precisely.

scholarly journals THE ANTIGENIC SIMILARITY OF HUMAN LOW DENSITY LIPOPROTEINS

1955 ◽  
Vol 102 (2) ◽  
pp. 105-118 ◽  
Author(s):  
Lawrence Levine ◽  
Dorothy L. Kauffman ◽  
Ray K. Brown
Keyword(s):  
Complement Fixation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Double Diffusion ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Antigenic Similarity ◽  
Cross Reactions

The following human low density lipoproteins were prepared: ß-lipoproteins of densities greater than 1.040 (A, B,C) a ß-lipoprotein of –S1·063 = 5 (D), a lipoprotein of –S1·063 = 19 (E), and a lipoprotein of –S1·063 = 70 (F). Data are presented which show the immunochemical homogeneity of the D lipoprotein rabbit-anti-D lipoprotein system. Cross-reactions between antibody to A and D lipoproteins and the above lipoproteins have been demonstrated by quantitative precipitation, quanitative complement fixation, and single and double diffusion in agar. The antigenic similarities appear to be associated with the protein portions of the molecule. The antisera produced did not differentiate the low density lipoprotein classes.

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

Chapter 10: Diagnosis

2019 ◽  
Author(s):  
Gerhard Dobler
Keyword(s):  
Cerebrospinal Fluid ◽  
Viral Encephalitis ◽  
Clinical Symptoms ◽  
Serological Tests ◽  
Specific Antibodies ◽  
Cross Reactions ◽  
Neuro Imaging ◽  
Tbev Infection

• TBE appears with non-characteristic clinical symptoms, which cannot be distinguished from oth-er forms of viral encephalitis or other diseases. • Cerebrospinal fluid and neuro-imaging may give some evidence of TBE, but ultimately cannot confirm the diagnosis. • Thus, proving the diagnosis “TBE” necessarily requires confirmation of TBEV-infection by detec-tion of the virus or by demonstration of specific antibodies from serum and/or cerebrospinal fluid. • During the phase of clinic symptoms from the CNS, the TBEV can only rarely be detected in the cerebrospinal fluid of patients. • Most routinely used serological tests for diagnosing TBE (ELISA, HI, IFA) show cross reactions resulting from either Infection with other flaviviruses or with other flavivirus vaccines.

Chapter 10: Diagnosis

2020 ◽  
Keyword(s):  
Cerebrospinal Fluid ◽  
Viral Encephalitis ◽  
Clinical Symptoms ◽  
Serological Tests ◽  
Specific Antibodies ◽  
Cross Reactions ◽  
Neuro Imaging ◽  
Tbev Infection

TBE appears with non-characteristic clinical symptoms, which cannot be distinguished from other forms of viral encephalitis or other diseases. Cerebrospinal fluid and neuro-imaging may give some evidence of TBE, but ultimately cannot confirm the diagnosis. Thus, proving the diagnosis “TBE” necessarily requires confirmation of TBEV-infection by detection of the virus or by demonstration of specific antibodies from serum and/or cerebrospinal fluid. During the phase of clinic symptoms from the CNS, the TBEV can only rarely be detected in the cerebrospinal fluid of patients. Most routinely used serological tests for diagnosing TBE (ELISA, HI, IFA) show cross reactions resulting from either infection with other flaviviruses or with other flavivirus vaccines.

INFLUENCE OF SPECIFIC ANTISERUM ON BIOLOGICAL EFFECTS OF HUMAN GONADOTROPHINS

1967 ◽  
Vol 56 (1_Suppl) ◽  
pp. S122
Author(s):  
P.-J. Czygan ◽  
D. Krebs ◽  
F. Lehmann ◽  
G. Bettendorf
Keyword(s):  
Biological Effects ◽  
Specific Antiserum

A Deep Pocket for the Truth of the Times

2017 ◽  
Author(s):  
Michael Germana
Keyword(s):  
Music Criticism ◽  
Invisible Man ◽  
The Past ◽  
Historical View ◽  
Sense Of Time ◽  
Cultural Forms ◽  
The Times ◽  
Musical Forms

Chapter 5 treats Ellison’s music criticism as an expression of his commitment to durational time and a critique of cultural forms like bebop that, in Ellison’s estimation, lend form to a discontinuous present. Rather than suggest, as many critics have, that Ellison was simply nostalgic for danceable swing music or hostile toward emerging musical forms, this chapter shows that Ellison’s primary criticism of bebop is that it formalizes a discontinuous sense of time and thereby affirms an historical view of the past structured by an analogous, sequentially static sense of time. Ellison’s problem with bebop, in other words, is neither musicological nor sociological, but temporal. Folk jazz and the blues, by contrast, affirm a durational view of time in the form of a “pocket” or groove entirely unlike the spatialized groove of history described in Invisible Man. In short, Ellison finds in musical grooves antidotes to the groove of history.

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