Molecular prevalence and genetic characterization of piroplasms in dogs from Tunisia

Parasitology ◽  
2016 ◽  
Vol 143 (12) ◽  
pp. 1622-1628 ◽  
Author(s):  
MOHAMED R. RJEIBI ◽  
SAFA AMAIRIA ◽  
MARIEM ROUATBI ◽  
FATMA BEN SALEM ◽  
MOEZ MABROUK ◽  
...  
Keyword(s):  
North Africa ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Chain Reaction ◽  
Companion Dogs ◽  
Central Tunisia ◽  
The North ◽  
Babesia Vogeli ◽  
Polymerase Chain

SUMMARYIn this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (PCR) to identifyBabesiaandTheileriaspecies in 200 dogs from Northern and Central Tunisia between spring and autumn 2014. The overall molecular prevalence for piroplasms was 14·5% ± 0·05 (29/200); PCR detected 2 species, namelyBabesia vogeliandTheileria annulatawith an overall prevalence of 12·5 ± 0·04 and 2% ± 0·02, respectively. No differences in the molecular prevalences ofB. vogeliwere revealed for age and sex (P> 0·05). The molecular prevalence ofB. vogeliwas significantly higher in central Tunisia (26·5% ± 0·01) compared with the North (9·6% ± 0·04) (P< 0·05). More working and companion dogs were infected byB. vogeli(25·8 ± 0·15 and 21·1% ± 0·13, respectively) in comparison with guarding dogs (1·8% ± 0·03) (P< 0·05). ConcerningT. annulata,no significant variation was observed for all studied risk factors (P> 0·05). Comparison of the partial sequences of18S rRNAandTams 1genes confirmed the presence of 2 novelB. vogeliandT. annulatagenotypes. This is the first molecular detection ofT. annulataand genetic characterization of dogs’ piroplasms in Tunisia. Further studies are needed to better assess the epidemiological feature of piroplasms infection in North Africa.

scholarly journals Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Meng Qi ◽  
Zilin Wei ◽  
Ying Zhang ◽  
Qiyuan Zhang ◽  
Juanfeng Li ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Genetic Characterization ◽  
Intestinal Parasites ◽  
Small Subunit ◽  
Kindergarten Children ◽  
Chain Reaction ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Southern Xinjiang

Abstract Background Blastocystis is one of the most common intestinal parasites in humans and various animals worldwide. Few studies are available regarding the genetic characterization of Blastocystis infections in humans in China. Methods In the present study, 609 fecal samples were collected from two- to six-year-old kindergarten children in southern Xinjiang and were examined by polymerase chain reaction (PCR). Results The infection rate of Blastocystis was 14.3% (87/609); no significant difference was observed among counties and between sexes. Blastocystis subtypes ST1 (n = 38), ST2 (n = 8), and ST3 (n = 41) were identified by sequence analysis of the small subunit ribosomal RNA gene. Genetic polymorphisms were observed at the intra-subtype level, including seven variations for ST1 (ST1A to ST1G), four for ST2 (ST2A to ST2D), and two for ST3 (ST3A and ST3B); with ST1F and ST2B being new variations. Conclusions ST1 and ST3 are the two common Blastocystis subtypes in the study area. More extensive studies in both humans and animals in different regions are needed to better characterize the transmission of Blastocystis.

The Polymerase Chain Reaction (PCR) in the routine genetic characterization of retinoblastoma: A tool for the clinical laboratory

1997 ◽  
Vol 41 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Domenico Mastrangelo ◽  
Nicola Squitieri ◽  
Stefano Bruni ◽  
Theodora Hadjistilianou ◽  
Renato Frezzotti
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Characterization ◽  
Clinical Laboratory ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiejun Peng ◽  
Shan Bu ◽  
Yueyan Yin ◽  
Mengying Hua ◽  
Kuangjie Zhao ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Genetic Characterization ◽  
Biological Properties ◽  
Capsicum Frutescens ◽  
Chain Reaction ◽  
Positive Sense ◽  
Polymerase Chain ◽  
Pepper Vein Yellows Virus ◽  
Generation Sequencing

Tombusvirus-like associated RNAs (tlaRNAs) are positive-sense single-stranded RNAs found in plants co-infected with some viruses of the genus Polerovirus. Pod pepper vein yellows virus (PoPeVYV) was recently reported as a new recombinant polerovirus causing interveinal yellowing, stunting, and leaf rolling in Capsicum frutescens plants at Wenshan city, Yunnan province, China. The complete genome sequence of its associated RNA has now been determined by next-generation sequencing and reverse transcription (RT) polymerase chain reaction (PCR). PoPeVYV-associated RNA (PoPeVYVaRNA) (GenBank Accession No. MW323470) has 2970 nucleotides and is closely related to other group II tlaRNAs, particularly tobacco bushy top disease-associated RNA (TBTDaRNA, GenBank Accession No. EF529625). In infection experiments on Nicotiana benthamiana and C. frutescens plants, synergism between PoPeVYVaRNA and PoPeVYV was demonstrated, leading to severe interveinal yellowing of leaves and stunting of plants. The results provide further information on the genetic and biological properties of the various agents associated with pepper vein yellows disease (PeVYD).

Cerebral Ovine Herpesvirus 2 Infection of Cattle Is Associated With a Variable Neuropathological Phenotype

2020 ◽  
pp. 030098582097049
Author(s):  
Melanie M. Hierweger ◽  
Céline L. Boujon ◽  
Ronja V. Kauer ◽  
Mireille Meylan ◽  
Torsten Seuberlich ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Viral Encephalitis ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
The Central Nervous System ◽  
Consistent Feature ◽  
Polymerase Chain ◽  
The Brain

Cross-species infection with ovine herpesvirus 2 (OvHV-2) in cattle causes malignant catarrhal fever (MCF). MCF may involve the central nervous system (CNS) with necrotizing arteritis and/or vasculitis described to be unique to MCF and discriminatory compared to other viral CNS infections. However, a systematic histopathological characterization of the neural form of MCF in cattle is lacking. We examined medulla oblongata ( n = 9) or the entire brain ( n = 9) of 18 cattle in which OvHV-2 was identified by quantitative polymerase chain reaction (qPCR), in order to pinpoint potential variations in neuropathology. In 2/18 animals (11%) no lesions were identified, while 16/18 cattle (89%) had brain lesions of varying severity. Presence and quantities of OvHV-2 nucleic acid were determined by in situ hybridization and qPCR, respectively, and were related to the severity of lesions. Fifteen of 18 animals (83%) showed vasculitis, which was mainly of the lymphohistiocytic type, while pathognomonic necrotizing arteritis was only rarely present. Neuroparenchymal lesions included gliosis and/or neuronal changes in 7/16 brains with lesions (44%). The number of CD3+ lymphocytes was highest in animals with simultaneous vascular and neuroparenchymal lesions and high viral genome load. In one animal, OvHV-2 was exclusively observed in CD3+ lymphocytes but not in neurons or microglia. In conclusion, the neuropathological phenotype of bovine MCF in the brain was variable. In some cases, lesions mimicked neurotropic viral encephalitis, while pathognomonic necrotizing arteritis was not a consistent feature of neural MCF. Therefore, molecular detection of OvHV-2 is warranted in the presence of nonsuppurative encephalitis and in the absence of necrotizing arteritis.

scholarly journals Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Rúben Fernandes ◽  
Paula Amador ◽  
Carla Oliveira ◽  
Cristina Prudêncio
Keyword(s):  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Generation Cephalosporin ◽  
Fourth Generation ◽  
Chain Reaction ◽  
E Coli ◽  
The North ◽  
Genetic Patterns ◽  
Polymerase Chain

Extended-spectrumβ-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, includingEscherichia coli(67.9%),Klebsiella pneumoniae(30.6%),Klebsiella oxytoca(0.5%),Enterobacter aerogenes(0.5%), andCitrobacter freundii(0.5%).β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboringblaESBLgenes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found forE. coliisolates and five clusters forK. pneumoniaestrains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.

Isolation and Molecular Characterization of Turkey Pox Virus from Maharashtra, India: A Review

2021 ◽  
Author(s):  
M.M. Pawade ◽  
P.P. Mhase ◽  
D.M. Muglikar ◽  
V.D. Lonkar ◽  
P.V. Mehere ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Virus Isolation ◽  
Viral Infections ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Chain Reaction ◽  
Slow Spreading ◽  
Polymerase Chain ◽  
Avian Pox

Avian pox diseases are contagious and slow spreading viral infections in birds. The present study was aim to, isolate and molecular characterization of turkeypox virus from a clinical case. Ten out of the twelve scab lesions sample collected from clinically suspected cases were positive for avian pox viurs (APV) based on virus isolation and polymerase chain reaction. We conducted genetic characterization of the APV strain. The phylogenetic analyses of P4b gene APV genome indicated that, avian poxviruses fragments sequenced in this study clustered along the A clade of avipoxviruses, genetically related to Indian fowl pox virus isolated from chicken, showing 99% homology.

Sign in / Sign up

Export Citation Format

Share Document