Larval development of Angiostrongylus chabaudi, the causative agent of feline angiostrongylosis, in the snail Cornu aspersum

Parasitology ◽  
2017 ◽  
Vol 144 (14) ◽  
pp. 1922-1930 ◽  
Author(s):  
V. COLELLA ◽  
M. A. CAVALERA ◽  
G. DEAK ◽  
V. D. TARALLO ◽  
C. M. GHERMAN ◽  
...  
Keyword(s):  
Intermediate Host ◽  
Developmental Stages ◽  
Land Snails ◽  
Morphological Description ◽  
Intermediate Hosts ◽  
Mammal Species ◽  
Larval Stages ◽  
Ecological Features ◽  
Cornu Aspersum ◽  
Angiostrongylus Chabaudi

SUMMARYNematodes of the Angiostrongylidae family, such as Angiostrongylus vasorum and Angiostrongylus cantonensis, may cause potentially life-threatening diseases in several mammal species. Alongside these well-known species, Angiostrongylus chabaudi has been recently found affecting the cardiopulmonary system of domestic and wild cats from Italy, Germany, Greece, Romania and Bulgaria. Nonetheless, significant gaps in the understanding of A. chabaudi epidemiology include the lack of information of species acting as intermediate host and of the morphological description of larval stages. Cornu aspersum (n = 30) land snails were infected with 100 first-stage larvae of A. chabaudi collected from a naturally infected wildcat in Romania. Larvae at different developmental stages were found in 29 out of 30 (96·7%) infected snails and a total of 282 (mean 9·8 ± 3·02 larvae per each specimen) were collected from the gastropods. Here we demonstrate that A. chabaudi develops in snails and report C. aspersum as potential intermediate host for this parasitic nematode. Findings of this study are central to understand the ecological features of feline angiostrongylosis and its epidemiology within paratenic and intermediate hosts.

scholarly journals Molecular data reshape our understanding of the life cycles of three digeneans (Monorchiidae and Gymnophallidae) infecting the bivalve, Donax variabilis: it’s just a facultative host!

Parasite ◽  
2021 ◽  
Vol 28 ◽  
pp. 34
Author(s):  
Kristina M. Hill-Spanik ◽  
Claudia Sams ◽  
Vincent A. Connors ◽  
Tessa Bricker ◽  
Isaure de Buron
Keyword(s):  
Gulf Of Mexico ◽  
Intermediate Host ◽  
Atlantic Coast ◽  
The United States ◽  
Life Cycles ◽  
Intermediate Hosts ◽  
Larval Stages ◽  
Complete Life Cycle ◽  
Morphological Criteria ◽  
Donax Variabilis

The coquina, Donax variabilis, is a known intermediate host of monorchiid and gymnophallid digeneans. Limited morphological criteria for the host and the digeneans’ larval stages have caused confusion in records. Herein, identities of coquinas from the United States (US) Atlantic coast were verified molecularly. We demonstrate that the current GenBank sequences for D. variabilis are erroneous, with the US sequence referring to D. fossor. Two cercariae and three metacercariae previously described in the Gulf of Mexico and one new cercaria were identified morphologically and molecularly, with only metacercariae occurring in both hosts. On the Southeast Atlantic coast, D. variabilis’ role is limited to being a facultative second intermediate host, and D. fossor, an older species, acts as both first and second intermediate hosts. Sequencing demonstrated 100% similarities between larval stages for each of the three digeneans. Sporocysts, single tail cercariae, and metacercariae in the incurrent siphon had sequences identical to those of monorchiid Lasiotocus trachinoti, for which we provide the complete life cycle. Adults are not known for the other two digeneans, and sequences from their larval stages were not identical to any in GenBank. Large sporocysts, cercariae (Cercaria choanura), and metacercariae in the coquinas’ foot were identified as Lasiotocus choanura (Hopkins, 1958) n. comb. Small sporocysts, furcocercous cercariae, and metacercariae in the mantle were identified as gymnophallid Parvatrema cf. donacis. We clarify records wherein authors recognized the three digenean species but confused their life stages, and probably the hosts, as D. variabilis is sympatric with cryptic D. texasianus in the Gulf of Mexico.

Life cycle studies on two Digenea, Paradistomum geckonum (Dicrocoeliidae) and Mesocoelium sociale (Mesocoeliidae), in geckonid lizards from Indonesia

1987 ◽  
Vol 65 (10) ◽  
pp. 2491-2497 ◽  
Author(s):  
Murray J. Kennedy ◽  
L. M. Killick ◽  
M. Beverley-Burton
Keyword(s):  
Life Cycle ◽  
Intermediate Host ◽  
Intermediate Hosts ◽  
Larval Stages ◽  
Hemidactylus Frenatus ◽  
Pulmonate Gastropod ◽  
First Intermediate Host

Life cycle studies of Paradistomum geckonum (Dicrocoeliidae) were attempted experimentally. The pulmonate gastropod Lamellaxis gracilis served as the first intermediate host; geckonid lizards (Cosymbotus platyurus, Gehyra mutilata, and Hemidactylus frenatus) served as definitive hosts. The life cycle of Mesocoelium sociale (Mesocoeliidae) was studied in naturally infected first intermediate hosts (L. gracilis, Huttonella bicolor) and experimentally in geckonid definitive hosts (C. platyurus, G. mutilata, and H. frenatus). Some naturally infected L. gracilis were infected concurrently with larval stages of both digeneans. Second intermediate hosts, presumed to be arthropods, were experimentally unnecessary. Metacercariae of P. geckonum were not found. Cercariae of M. sociale formed encysted metacercariae in the same individual snails.

scholarly journals Larval trematodes in freshwater gastropods from Mato Grosso, Brazil: diversity and host-parasites relationships

2013 ◽  
Vol 13 (4) ◽  
pp. 34-38 ◽  
Author(s):  
Aline Carvalho de Mattos ◽  
Maria Fernanda Furtado Boaventura ◽  
Monica Ammon Fernandez ◽  
Silvana Carvalho Thiengo
Keyword(s):  
Schistosoma Mansoni ◽  
Intermediate Host ◽  
Intermediate Hosts ◽  
Mato Grosso ◽  
Freshwater Gastropods ◽  
Larval Stages ◽  
Pomacea Maculata ◽  
Surrounding Areas ◽  
First Time

A survey for freshwater gastropods carrying trematodes parasites was conducted in Manso Dam and the surrounding areas frequented by tourist, focusing particularly on the Pantanal region. Infected snails were recovered from twelve of the eighteen investigated municipalities and forty-one cercaria-snail pairings were recorded. Among these pairings were several first records of snails serving as intermediate hosts for trematodes in Brazil including Biomphalaria amazônica Paraense, 1966, Biomphalaria occidentalis Paraense, 1981, Marisa planogyra Pilsbry, 1933, Pomacea maculata Perry, 1830, Pomacea scalaris (d'Orbigny, 1835) and Gundlachia radiata (Guilding, 1828). Echinostomatidae and Strigeidae were the most common trematode families (ca. 47%) and the greatest diversity of larvae were obtained from Drepanotrema lucidum (Pfeiffer, 1839). Paramphistomatidae, Schistosomatidae or Spirorchiidae and Notocotylidae or Pronocephalidae were recovered in D. lucidum for the first time extending the number of families which use this gastropod as intermediate host. Although no specimens were found harboring larval stages of Schistosoma mansoni Sambon, 1907 other trematode larvae were discovered, including the Schistosomatidae Brevifurcate apharingeate cercaria that can cause dermatitis in humans. Continued studies on the taxonomy and biology of trematodes are essential to better understand the biodiversity of these parasites as well as the epidemiological aspects for control of associated zoonosis.

Molecular evidence linking the larval and adult stages of Mexiconema cichlasomae (Dracunculoidea: Daniconematidae) from Mexico, with notes on its phylogenetic position among Dracunculoidea

2018 ◽  
Vol 93 (05) ◽  
pp. 580-588 ◽  
Author(s):  
A.L. May-Tec ◽  
A. Martínez-Aquino ◽  
M.L. Aguirre-Macedo ◽  
V.M. Vidal-Martínez
Keyword(s):  
Life Cycle ◽  
Developmental Stages ◽  
Gravid Female ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Molecular Evidence ◽  
Intermediate Hosts ◽  
Larval Stages ◽  
The Family ◽  
Cichlasoma Urophthalmus

AbstractWe describe the larval developmental stages and life cycle of the dracunculid nematodeMexiconema cichlasomaein both the intermediate,Argulus yucatanus(Crustacea: Branchiura), and definitive hosts,Cichlasoma urophthalmus(Perciformes: Cichlidae), from the Celestun tropical coastal lagoon, Yucatan, Mexico. The morphological analyses showed significant differences between the total length of L1 found inM. cichlasomaegravid female and L2–L3 inA. yucatanus.This result indicates that theM. cichlasomaelarval development occurs in the intermediate host. We obtained sequences from the small subunit (SSU) ribosomal marker from larval stages ofM. cichlasomaeinA. yucatanusand adult nematodes inC. urophthalmus. Our morphological and molecular results support conspecificity betweenM. cichlasomaelarvae inA. yucatanusand the adult stages inC. urophthalmus. We briefly discuss the phylogenetic position ofM. cichlasomaeamong the Daniconematidae, and provide evidence of the monophyly of the daniconematids associated with branchiurid intermediate hosts. Based on the phylogenetic results, we support the transfer of theMexiconemagenus to the family Skrjabillanidae and do not support the lowering of family Daniconematidae to subfamily.

Children, snails and worms: the Brachylaima cribbi story

2016 ◽  
Vol 37 (1) ◽  
pp. 30 ◽  
Author(s):  
Andrew R Butcher
Keyword(s):  
Life Cycle ◽  
South America ◽  
South Australia ◽  
Land Snails ◽  
Intermediate Hosts ◽  
Larval Stages ◽  
Wide Range ◽  
Terrestrial Life ◽  
North And South America ◽  
North And South

Brachylaimids are parasitic trematode fluke worms that have a terrestrial life cycle involving land snails and slugs as the first and/or second intermediate hosts for the cercarial and metacercarial larval stages. A wide range of mammals, birds, reptiles and amphibians are the definitive hosts for the adult worm. Brachylaima spp. have been reported from most continents including Europe, Africa, Asia, North and South America and Australia. There are over 70 described species in the genus with seven species indigenous to Australia. Although Brachylaima spp. are a cosmopolitan terrestrial trematode they have not been recorded to infect humans other than the three Brachylaima cribbi infections reported in two children and an adult from South Australia.

Observations on the Larval Stages of Muellerius capillaris within the Intermediate Hosts Agriolimax agrestis and A. reticulatus

1957 ◽  
Vol 31 (1-2) ◽  
pp. 1-16 ◽  
Author(s):  
J. H. Rose
Keyword(s):  
Intermediate Host ◽  
Infective Stage ◽  
Intermediate Hosts ◽  
Rate Of Development ◽  
Larval Stages ◽  
Infective Larvae ◽  
Artificial Digestion ◽  
Do So ◽  
Muellerius Capillaris

1. The rate of development of the larvae of M. capillaris is slowed down as the temperature is lowered; at 25°C. the infective stage is reached after 8 days; at 5°C. after 98 days.2. The differences between “pre-infective” and “infective” larvae, as described by Gerichter (1948), have been confirmed.3. Larvae at the “infective” stage can withstand artificial digestion, but “pre-infective” larvae are unable to do so.4. The infective larvae can survive for long periods within the foot of the intermediate host.5. Pre-infective larvae can survive for seven days after the death of the intermediate host; infective larvae can live for sixteen days.6. Naturally-infected slugs were found throughout the greater part of the year. 13.5 per cent of the slugs collected were infected but in most instances only a few larvae were present in any one slug.

Differences in snail ecology lead to infection pattern variation of Echinostoma spp. larval stages

2014 ◽  
Vol 59 (3) ◽  
Author(s):  
Michael Zimmermann ◽  
Kyle Luth ◽  
Gerald Esch
Keyword(s):  
United States ◽  
Intermediate Host ◽  
Feeding Ecology ◽  
Intermediate Hosts ◽  
Midwestern United States ◽  
Host Behavior ◽  
Larval Stages ◽  
Infection Pattern

AbstractThe infection patterns of parasites are often tied to host behavior. Although most studies have investigated definitive hosts and their parasites, intermediate host behavior may play a role in shaping the distribution and accumulation of parasites, particularly the larval stages. In an attempt to answer this question, more than 4,500 pulmonate snails were collected from 11 states in the mid-Atlantic and Midwestern United States in the summer of 2012. These snails were necropsied and echinostome metecercariae were commonly observed infecting the snails as 2nd intermediate hosts (20.0%). The snails included species of 3 genera with distinct differences in the infection patterns of Echinostoma spp. metacercariae among them. Physa spp. (comprising of P. acuta and P. gyrina) snails exhibited a significantly higher prevalence of infection (23.5%) than both Lymnaea columella (11.6%) and Helisoma spp. (comprising of H. anceps and H. trivolvis) (14.2%; P < 0.05), with no difference in prevalence observed between the latter 2 genera (P > 0.05). The intensity of metacercariae within the snail hosts was significantly different between the 3 genera (P < 0.05), with L. columella having the highest intensity (24.3 ± 5.6), followed by Physa spp. (15.2 ± 1.5) and Helisoma spp. (5.0 ± 0.9). Differences in prevalence and intensity were also observed when the different snail families co-habited the same body of water. The disparities in infection patterns are likely due to distinct differences in the behavioral and feeding ecology of the snail hosts.

The development ofMoniezia expansain the intermediate host

Parasitology ◽  
1938 ◽  
Vol 30 (4) ◽  
pp. 491-501 ◽  
Author(s):  
Horace W. Stunkard
Keyword(s):  
Intermediate Host ◽  
Developmental Stages ◽  
Body Cavity ◽  
The Body ◽  
Intermediate Hosts

When eggs ofMonieza expansaare fed to galumnid mites, the onchospheres emerge in the intestine and migrate to the body cavity. Here they undergo metamorphosis and develop into cysticercoids. The dissection of mites, at various intervals after exposure, has yielded a successive series of developmental stages. Protocols of the experiments and photographs of living larvae are presented. These results demonstrate that mites serve as intermediate hosts ofMoniezia, and probably also of other anoplocephaline cestodes.

The paramphistomes (Trematoda) of Australian ruminants.

1953 ◽  
Vol 1 (2) ◽  
pp. 193 ◽  
Author(s):  
PH Durie
Keyword(s):  
Life Cycle ◽  
Intermediate Host ◽  
Marked Effect ◽  
Mantle Cavity ◽  
Developmental Period ◽  
Intermediate Hosts ◽  
Minimum Period ◽  
Rate Of Development ◽  
Larval Stages

A description of the development at 27�C. of Ceylonocotyle streptocoelium (Fischoeder 1901) Nasmark 1937, a paramphistome from the rumen and reticulum of sheep and cattle in Australia, is given in detail. Eggs hatched after 16 days' incubation and miracidia penetrated the intermediate host, a planorbid snail, Glyptanisus gilberti Dunker 1848, through the mantle cavity. The development of the larval stages of the parasite within the intermediate host was completed 34 days after infection, when cerariae were secreted by snails. Cysts of C. streptocoelium fed to sheep and cattle showed that flukes reached maturity in the rumen in 48 and 56 days respectively. The life cycle of the fluke, therefore, was completed in a minimum period of approximately 3.5 months under these conditions. It was found that temperature had a marked effect on the rate of development of larval stages within the molluscan intermediate host, the developmental period at 20�C. being twice as long as that at 27�C. The various stages occurring in the molluscan intermediate host are described in detail. The development of Paramphistomum ichikauiai Fukui 1922 is compared with that of C. streptocoelium. The rate of development was found to be more rapid than for C. streptocoelium under the same conditions. Eggs hatched after 12 days incubation and cercariae were secreted from the intermediate host, Segnitilia alphena Iredale 1943, 25 days after infection. Flukes were found to reach maturity in sheep 49-51 days after cysts had been fed. The eggs and intra-molluscan stages are similar in both species, but the cercariae may be readily distinguished by anatomical differences in their excretory systems. The life cycle in this species takes approximately 3 months to complete under these conditions. The molluscan intermediate hosts of the two species of amphistomes are described and notes on their bionomics are given.

Muellerius capillaris in north-east Zaire: prevalence in sheep and goats and determination of intermediate hosts

1989 ◽  
Vol 63 (4) ◽  
pp. 298-301
Author(s):  
J. Cabaret ◽  
C. Chartier
Keyword(s):  
Intermediate Host ◽  
Land Snails ◽  
Intermediate Hosts ◽  
Natural Conditions ◽  
Sheep And Goats ◽  
Adult Sheep ◽  
North East ◽  
Muellerius Capillaris

ABSTRACTMuellerius capillaris was the only species of nematode recovered from the lungs of young and adult sheep and goats of north-east Zaire. The prevalences were of 19% (sheep) and 32% (goats). The slug Atoxon pallens contained larvae in natural conditions and represents a potential intermediate host. The land snails Achatinidae (Achatina stuhlmanni and Limicolaria spp.) could not be experimentally infected with M. capillaris larvae.

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