XII.—Electron Microscopy of the Epithelial Cells of the Mid-gut and Hepatic Cæca of Blatta Orientalis

1961 ◽  
Vol 68 (2) ◽  
pp. 162-170
Author(s):  
L. T. Threadgold ◽  
R. A. R. Gresson
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Light Microscopy ◽  
Electron Micrographs ◽  
Anterior Part ◽  
Blatta Orientalis ◽  
Lateral Margins

SynopsisElectron micrographs of the anterior part of the mid-gut and hepatic cæca of Blatta orientalis Linn, show epithelial cells in both secretory and absorptive cycles. The epithelial cells possess microvilli and their lateral margins are provided with irregularly shaped projections that interdigitate with neighbouring cells. Vacuole-like structures were observed in association with the mitochondria of absorptive and degenerating cells. These vacuoles, it is suggested, represent degeneration products. Bundles of saccules and associated groups of vacuoles and vesicles represent the Golgi rods and granules of light microscopy. Epithelial cells engaged in absorption differ from those in which the elaboration of secretion is taking place, in the arrangement of the Golgi saccules and vacuoles, in the size of the Golgi vacuoles, in the distribution of the mitochondria, and in the possession of a less extensive endoplasmic reticulum.

Extrusion of Nuclear Material During Oogenesis in Blatta Orientalis

1962 ◽  
Vol s3-103 (62) ◽  
pp. 141-145
Author(s):  
R.A. R. GRESSON ◽  
L. T. THREADGOLD
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Nuclear Membrane ◽  
Close Association ◽  
Nuclear Material ◽  
Blatta Orientalis ◽  
Nucleolar Material

That nucleolar material is extruded to the cytoplasm of the young oocyte of Blatta orientalis is confirmed by means of electron microscopy. The nucleolus and nucleolar extrusions are shown to contain RNA. In addition to the nucleolar extrusions, vesicle-like structures originate in the nuclear membrane and from there pass into the cytoplasm where they become indistinguishable from elements of the endoplasmic reticulum. When the nucleolar extrusions reach the cytoplasm they increase in size, come into close association with a few mitochondria, and migrate towards the periphery of the cell. It is concluded that the emission of material from the nucleolus and the passage of vesicles from the nuclear membrane to the cytoplasm are necessary prerequisites for the process of vitellogenesis.

Tannic Acid as Fixative and Stain for Amyloid Tissues

1977 ◽  
Vol 35 ◽  
pp. 542-543
Author(s):  
O. G. Rodgers ◽  
T. Shirahama ◽  
A. S. Cohen
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Tannic Acid ◽  
Electron Micrographs ◽  
Amyloid Fibrils ◽  
Fibrillar Structure ◽  
High Magnification ◽  
Animal Kingdom ◽  
Morphologic Characteristics ◽  
Liver And Kidney

Amyloid is a protein deposited in a variety of organs in the disorder amyloidosis which is widely distributed in the animal kingdom. The identification of this substance and diagnosis of the disease is largely dependent on its morphologic characteristics and tinctorial properties by light microscopy and the fibrillar structure by electron microscopy (1). In electron micrographs prepared conventionally, very often the amyloid fibrils show low electron density, making it difficult to identify small airyloid deposits when scanning by low magnification, and difficult to analyze ultrastructural details at high magnification. After testing various fixatives and stains to achieve good fixation and staining of the anyloid fibrils, we have found that the use of tannic acid as a fixative (2) may satisfy these objectives.Various tissues (skin, tongue, prostate, spleen, liver and kidney) were obtained at biopsy, surgery or autopsy from patients with primary, secondary, myelcma-associated or hereditary amyloidosis. Spleen, liver and kidney from CBA/J mice in which amyloidosis was induced by daily casein injections were also used.

Postnatal Development of the Feline End Bulb of Held

1985 ◽  
Vol 43 ◽  
pp. 492-493
Author(s):  
S. A. Larsen ◽  
George D. Pappas
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Postnatal Development ◽  
Electron Micrographs ◽  
Cochlear Nucleus ◽  
Normal Development ◽  
Computer Assisted ◽  
Anteroventral Cochlear Nucleus ◽  
Adult Cats ◽  
Large Spherical

Maturation of the end bulb of Held (EBH) in the anteroventral cochlear nucleus (AVCN) was studied to determine what degree of plasticity is found in a synapse undergoing maturation, at the same time that the recipient neuron is increasing in size. Although the normal development of the neurons in the cochlear nucleus has been studied by Larsen and Morest, the normal development of the EBH has not been reported at the EM level.The EBH was studied in kittens ranging in age from birth to 60 days postpartum and in adult cats using electron microscopy and Golgi preparations with light microscopy. At selected developmental ages (birth, alternate days starting at the second day of life and extending throughout the first postnatal month, and adult), serial-sectioned electron micrographs of the EBH and its recipient neuron, the large spherical cell (LSC), were traced using computer-assisted morphometry.

The Electron Microscopy of the ‘Golgi Apparatus’in the Purkinje Cells of Owls

1960 ◽  
Vol s3-101 (56) ◽  
pp. 389-394
Author(s):  
S. K. MALHOTRA ◽  
G. A. MEEK
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Purkinje Cells ◽  
Electron Micrographs ◽  
Optical Microscope

The classical site of the ‘Golgi apparatus’, the Purkinje cells of the cerebellum of owls, has been examined by electron microscopy. The greater part of the cytoplasm consists of aggregates of closely packed granular membranes ofendoplasmic reticulum. The objects described by Dalton and Felix in electron micrographs and called by them the Golgi apparatus are rarely seen in these preparations. It seems likely that the ‘Golgi apparatus’ of this cell as seen in the optical microscope is formed by the deposition of silver or osmium on the membranes of the endoplasmic reticulum, which form a network throughout the cytoplasm of this cell.

scholarly journals Immunocytochemical localization of ornithine transcarbamylase in rat intestinal mucosa. Light and electron microscopic study.

1988 ◽  
Vol 36 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Y Hamano ◽  
H Kodama ◽  
M Yanagisawa ◽  
Y Haraguchi ◽  
M Mori ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Light Microscopy ◽  
Intestinal Mucosa ◽  
Intestinal Epithelial Cells ◽  
Protein A ◽  
Intestinal Epithelial ◽  
Electron Microscopic ◽  
Small Intestinal

We investigated light and electron microscopic localization of ornithine transcarbamylase (OTC) in rat intestinal mucosa. In the immunoblotting assay of OTC-related protein, a single protein band with a molecular weight of about 36,500 is observed in extracts of liver and small intestinal mucosa but is not observed in those of stomach and large intestine. For light microscopy, tissue slices of the digestive system were embedded in Epon and stained by using anti-bovine OTC rabbit IgG and the immunoenzyme technique. For electron microscopy, slices of these and the liver tissues were embedded in Lowicryl K4M and stained by the protein A-gold technique. By light microscopy, the absorptive epithelial cells of duodenum, jejunum, and ileum stained positively for OTC, but stomach, large intestine, rectum, and propria mucosa of small intestine were not stained. Electron microscopy showed that gold particles representing the antigenic sites for OTC were confined to the mitochondrial matrix of hepatocytes and small intestinal epithelial cells. However, the enzyme was detected in mitochondria of neither liver endothelial cells, submucosal cells of small intestine, nor large intestinal epithelial cells. Labeling density of mitochondria in the absorptive epithelial cells of duodenum, jejunum, and ileum was about half of that in liver cells.

scholarly journals THE USE OF THIOL-SUBSTITUTED CARBOXYLIC ACIDS AS HISTOCHEMICAL SUBSTRATES

1965 ◽  
Vol 13 (8) ◽  
pp. 611-628 ◽  
Author(s):  
MARY BELL ◽  
RUSSELL J. BARRNETT
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Lipid Metabolism ◽  
Light Microscopy ◽  
Nuclear Envelope ◽  
Carboxylic Acids ◽  
Structural Level ◽  
Low Concentrations ◽  
Hydrolysis Of ◽  
Thiolacetic Acid

Thiobutyric, thiocaproic and thiocaprylic acids were synthesized, and enzyme histochemical methods were developed using these thiol-substituted carboxylic acids, as well as thiolacetic acid, as substrates with Pb++ as the capture reagent. The localization of final product of these histochemical reactions, PbS, was studied and compared in a variety of tissues with light microscopy. The enzymatic activities demonstrated were sensitive to low concentrations of E600. The localization of these reactions in several intensely reactive tissues, liver, testis and intestine, were also studied with electron microscopy. At a fine structural level, the final product was deposited primarily in relation to the membranous elements of the smooth and rough varieties of the endoplasmic reticulum, including the nuclear envelope, and of mitochondria. The results of these experiments are discussed, including the possible identity of the enzymes concerned with the hydrolysis of the thiol-substituted substrates. It was suggested that at least three activities were demonstrated in these experiments, one of which was the B type esterase of microsomes, and all of which functioned in lipid metabolism.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE ENDOPLASMIC RETICULUM IN NEWT NOTOCHORD CELLS AFTER DISTURBANCE WITH ULTRASONIC TREATMENT AND SUBSEQUENT REGENERATION

1964 ◽  
Vol 20 (1) ◽  
pp. 175-183 ◽  
Author(s):  
G. G. Selman ◽  
A. Jurand
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Ultrasonic Treatment ◽  
Electron Microscope Study ◽  
Triturus Alpestris ◽  
Notochord Cells ◽  
Parallel Arrays ◽  
Subsequent Regeneration

Ultrasonic treatment of the tails of Triturus alpestris tadpoles, at intensities of 8 to 15 watts/cm2, at 1 megacycle/sec., for 5 minutes, disrupted the epidermis and caused pycnosis in individual cells of the muscle and neural tube, but caused no damage to the notochord that could be detected by light microscopy. Electron microscopy showed that this ultrasonic treatment disordered nearly all the endoplasmic reticulum (ER) of the notochord cells into irregularly rounded vesicles, but within 3 hours after treatment some parallel arrays of normal endoplasmic reticulum were seen near, and continuous with, the outer nuclear membrane. In addition, a re-ordering of the previously disordered ER took place throughout the cytoplasm, in some cases. A classification was made of the state of the ER as shown in electron micrographs of material fixed immediately, 3, and 24 hours after treatment. This showed that more than half the total endoplasmic reticulum in notochord cells was normal again by 24 hours after treatment.

scholarly journals Ultrastructure of the Epithelial Cells Associated with Tooth Biomineralization in the Chiton Acanthopleura hirtosa

2009 ◽  
Vol 15 (2) ◽  
pp. 154-165 ◽  
Author(s):  
Jeremy A. Shaw ◽  
David J. Macey ◽  
Lesley R. Brooker ◽  
Edward J. Stockdale ◽  
Martin Saunders ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epithelial Cells ◽  
Light Microscopy ◽  
Mineralization Process ◽  
Conventional Transmission Electron Microscopy ◽  
Physiological Conditions ◽  
Microwave Assisted ◽  
Transmission Electron ◽  
Extracellular Compartment

AbstractThe cusp epithelium is a specialized branch of the superior epithelium that surrounds the developing teeth of chitons and is responsible for delivering the elements required for the formation of biominerals within the major lateral teeth. These biominerals are deposited within specific regions of the tooth in sequence, making it possible to conduct a row by row examination of cell development in the cusp epithelium as the teeth progress from the unmineralized to the mineralized state. Cusp epithelium from the chiton Acanthopleura hirtosa was prepared using conventional chemical and microwave assisted tissue processing, for observation by light microscopy, conventional transmission electron microscopy (TEM) and energy filtered TEM. The onset of iron mineralization within the teeth, initiated at row 13, is associated with a number of dramatic changes in the ultrastructure of the apical cusp cell epithelium. Specifically, the presence of ferritin containing siderosomes, the position and number of mitochondria, and the structure of the cell microvilli are each linked to aspects of the mineralization process. These changes in tissue development are discussed in context with their influence over the physiological conditions within both the cells and extracellular compartment of the tooth at the onset of iron mineralization.

Marginal bands in camel erythrocytes

1979 ◽  
Vol 36 (1) ◽  
pp. 97-107
Author(s):  
W.D. Cohen ◽  
N.B. Terwilliger
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Electron Density ◽  
Electron Micrographs ◽  
Iron Content ◽  
Phase Contrast ◽  
Transmission Electron ◽  
Marginal Band ◽  
Whole Mounts

The elliptical, anucleate erythrocytes of camels have been examined for the presence of marginal bands and their constituent microtubules. Lysis of erythrocytes under microtubule-stabilizing conditions readily revealed marginal bands in at least 3 % of the cells, as observed by phase-contrast and darkfield light microscopy. Microtubules plus a marginal band-encompassing network of material are visible in lysed cell whole mounts with transmission electron microscopy. Marginal band microtubules are also evident in electron micrographs of thin-sectioned camel erythrocytes identifiable as reticuloyctes on the basis of submaximal electron density (reduced haemoglobin iron content) and presence of polysomes. The results suggest that marginal bands may be involved in morphogenesis of camel erythrocytes but are not required for maintenance of their ellipticity after cells are fully differentiated.

scholarly journals Progressive Depletion of Rough Endoplasmic Reticulum in Epithelial Cells of the Small Intestine in Monosodium Glutamate Mice Model of Obesity

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Kazuhiko Nakadate ◽  
Kento Motojima ◽  
Tomoya Hirakawa ◽  
Sawako Tanaka-Nakadate
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Rough Endoplasmic Reticulum ◽  
Defense Mechanism ◽  
Monosodium Glutamate ◽  
Light And Electron Microscopy ◽  
Obese Mice ◽  
Pathological Changes

Chronic obesity is a known risk factor for metabolic syndrome. However, little is known about pathological changes in the small intestine associated with chronic obesity. This study investigated cellular and subcellular level changes in the small intestine of obese mice. In this study, a mouse model of obesity was established by early postnatal administration of monosodium glutamate. Changes in body weight were monitored, and pathological changes in the small intestine were evaluated using hematoxylin-eosin and Nissl staining and light and electron microscopy. Consequently, obese mice were significantly heavier compared with controls from 9 weeks of age. Villi in the small intestine of obese mice were elongated and thinned. There was reduced hematoxylin staining in the epithelium of the small intestine of obese mice. Electron microscopy revealed a significant decrease in and shortening of rough endoplasmic reticulum in epithelial cells of the small intestine of obese mice compared with normal mice. The decrease in rough endoplasmic reticulum in the small intestine epithelial cells of obese mice indicates that obesity starting in childhood influences various functions of the small intestine, such as protein synthesis, and could impair both the defense mechanism against invasion of pathogenic microbes and nutritional absorption.

Sign in / Sign up

Export Citation Format

Share Document