scholarly journals Using CCD’s in Introductory-Level College Astronomy Laboratories

1990 ◽  
Vol 105 ◽  
pp. 142-144
Author(s):  
Thomas J. Balonek
Keyword(s):  
Dark Current ◽  
Software Package ◽  
Multiple Access ◽  
Ccd Camera ◽  
Analysis Software ◽  
Camera System ◽  
Black And White ◽  
Standard Data ◽  
College Astronomy ◽  
Cooled Ccd

Our university recently purchased a liquid-nitrogen-cooled CCD camera system (from Photometrics Ltd., Tucson, Arizona) which has been installed on our campus’ 40-cm multiple access (Cassegrain/Newtonian) telescope. Images are reduced online at the observatory using Photometrics’ microcomputer-based analysis software package, which includes operations for standard data acquisition and initial stages of data reduction — including corrections for bias, dark current, and flat fielding. Images are displayed on a 256-level-gray-scale black and white monitor. Additional post-processing can be done either on the CCD system’s computer at the observatory or on IBM-AT/PC’s located both at the observatory and in the laboratory.

A Peltier-cooled CCD Camera

1991 ◽  
Vol 9 (1) ◽  
pp. 158-159 ◽  
Author(s):  
B. D. Carter ◽  
M. C. B. Ashley
Keyword(s):  
Dark Current ◽  
Cooling System ◽  
Ccd Camera ◽  
Radiative Heating ◽  
Peltier Effect ◽  
Charge Coupled Device ◽  
Automated Imaging ◽  
Cooled Ccd ◽  
Ccd Detectors ◽  
Careful Design

AbstractWe describe the application of Peltier effect cooling to charge coupled device (CCD) detectors. We are developing this technique to produce a CCD camera which requires low maintenance, yet has sufficiently small dark-current for long exposure imaging. This camera will be used in an automated imaging telescope at Siding Spring Observatory. The design principles used to maximise cooling of the detector, and hence minimise dark-current, are discussed. A small dark-current can be obtained only if great care is taken to reduce or eliminate convective, conductive and radiative heating of the chip. In addition, a path of high thermal conductivity must be provided for the heat removed from the CCD. A recent laboratory test of our cooling system demonstrates that careful design can lead to sufficiently low CCD dark-current for many astronomical applications.

Five-Dimensional Microscopy using Widefield-Deconvolution: Practical Considerations and Biological Applications

1996 ◽  
Vol 54 ◽  
pp. 268-269
Author(s):  
W.F. Marshall ◽  
K. Oegema ◽  
J. Nunnari ◽  
A.F. Straight ◽  
D.A. Agard ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
High Speed ◽  
Laser Scanning ◽  
Ccd Camera ◽  
Image Plane ◽  
Time Lapse ◽  
Laser Scanning Confocal Microscopy ◽  
Three Dimensions ◽  
Excitation Light ◽  
Cooled Ccd

The ability to image cells in three dimensions has brought about a revolution in biological microscopy, enabling many questions to be asked which would be inaccessible without this capability. There are currently two major methods of three dimensional microscopy: laser-scanning confocal microscopy and widefield-deconvolution microscopy. The method of widefield-deconvolution uses a cooled CCD to acquire images from a standard widefield microscope, and then computationally removes out of focus blur. Using such a scheme, it is easy to acquire time-lapse 3D images of living cells without killing them, and to do so for multiple wavelengths (using computer-controlled filter wheels). Thus, it is now not only feasible, but routine, to perform five dimensional microscopy (three spatial dimensions, plus time, plus wavelength).Widefield-deconvolution has several advantages over confocal microscopy. The two main advantages are high speed of acquisition (because there is no scanning, a single optical section is acquired at a time by using a cooled CCD camera) and the use of low excitation light levels Excitation intensity can be much lower than in a confocal microscope for three reasons: 1) longer exposures can be taken since the entire 512x512 image plane is acquired in parallel, so that dwell time is not an issue, 2) the higher quantum efficiently of a CCD detect over those typically used in confocal microscopy (although this is expected to change due to advances in confocal detector technology), and 3) because no pinhole is used to reject light, a much larger fraction of the emitted light is collected. Thus we can typically acquire images with thousands of photons per pixel using a mercury lamp, instead of a laser, for illumination. The use of low excitation light is critical for living samples, and also reduces bleaching. The high speed of widefield microscopy is also essential for time-lapse 3D microscopy, since one must acquire images quickly enough to resolve interesting events.

Gamma-ray imaging with an image intensifier and a cooled CCD camera

2001 ◽  
Author(s):  
Naoki Saitoh ◽  
Kenro Kuroki ◽  
Kenji Kurosawa ◽  
Norimitsu Akiba
Keyword(s):  
Gamma Ray ◽  
Image Intensifier ◽  
Ccd Camera ◽  
Gamma Ray Imaging ◽  
Cooled Ccd

scholarly journals Multiscale 2D-SPR Biosensing for Cell Chips

2007 ◽  
Vol 19 (5) ◽  
pp. 519-523 ◽  
Author(s):  
Masayasu Suzuki ◽  
◽  
Toyohiro Ohshima ◽  
Shintaro Hane ◽  
Yasunori Iribe ◽  
...  
Keyword(s):  
Protein A ◽  
Cell Activity ◽  
Ccd Camera ◽  
Pdms Film ◽  
Sensor Film ◽  
Spr Imaging ◽  
Measurement Protocol ◽  
A Cell ◽  
Cooled Ccd ◽  
Imaging Sensor

Evaluating cell activity and functions in different-sized cell chambers requires multiscale sensing. We have been developing multiscale biosensing applied from 10 µm to 1 mm. We measured mouse IgG in micro wells using a high-resolution two-dimensional surface plasmon resonance (SPR) imaging affinity sensor. This sensor uses high refractive optics, a 1X to 7X microscopic lens, and a cooled CCD camera. The micro-well array was prepared with a PDMS film on gold sensor film. Protein A immobilized on sensor film was used for IgG recognition. SPR sensitivity was dramatically decreased with 10 and 8.5 µm microwells. To improve sensor sensitivity, we optimized the sensor’s measurement angle and exposure time, enabling mouse IgG to be detected in wells of 1 mm, 30 µm, and 10 µm using the same 2D-SPR imaging sensor and measurement protocol. These results show the feasibility of multiscale biosensing use in antibody production in a micro well or a cell chamber.

Optical Analysis of Pulse Combustion Using Shadowgraph and Planar CH-LIF Imaging Technique

2000 ◽  
Vol 123 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Yojiro Ishino ◽  
Tatsuya Hasegawa ◽  
Shigeki Yamaguchi ◽  
Norio Ohiwa
Keyword(s):  
Large Scale ◽  
Ccd Camera ◽  
Pulsation Period ◽  
Planar Imaging ◽  
Camera System ◽  
Band Emission ◽  
Pulse Combustor ◽  
Pulse Combustion ◽  
Combustion Processes ◽  
Ch Radical

Planar imaging of laser-induced fluorescence of CH radical is made to examine combustion processes in a valveless pulse combustor. An excimer-pumped dye laser tuned to a wavelength of 387 nm is used to excite the R1N″=6 line of (0,0) band of the B2Σ−−X2Π system of CH radical, and an image-intensified CCD camera system is used to detect the (0,1) band emission at around 435 nm. According to the CH-LIF images, it is found that the progress in combustion during a pulsation period is expressed by the enlargement and breakup of the earlobe-shaped flame front along the outline of a pair of large-scale eddies of fresh mixture.

Sign in / Sign up

Export Citation Format

Share Document