Effect of different diet supplements in the pre- and post-partum period on reproductive performance in the dairy cow

2001 ◽  
Vol 26 (2) ◽  
pp. 313-321 ◽  
Author(s):  
N. H. Gardner ◽  
C. K. Reynolds ◽  
R.H. Phipps ◽  
A.K. Jones ◽  
D.E. Beever
Keyword(s):  
Sugar Beet ◽  
Dairy Cows ◽  
Reproductive Performance ◽  
Post Partum ◽  
Late Gestation ◽  
Diet Supplements ◽  
Group 2 ◽  
The Impact ◽  
Ration Group ◽  
Group 1

AbstractThe study compared the impact of feeding different energy supplements (barley, molassed sugar beet and fat) prior to calving and the effects of feeding supplemental fat post-partum, on subsequent production and reproductive efficiency of dairy cows. Forty-eight multiparous Holstein-Friesian dairy cows were assigned to one of two groups, six weeks prior to expected calving date and fed a grass based total mixed ration according to ME requirements for late gestation. Group 1 was supplemented with barley (B) or molassed sugar beet feed (SB) prior to calving and was then given a high starch lactation ration. Group 2 was supplemented with either fat (F) or no supplement (C) pre partum, and was then given a similar lactation ration as Group 1 but supplemented with fat. Lactation rations were fed through to week-20 post partum and the cows were monitored during this period. Milk yield (P<0.002) and milkfat (P<0.02) production were higher and milk protein concentration (P<0.001) was lower in Group 2. The number of days to first rise in progesterone following parturition was greater (P<0.01) in Group 2. Due to the design of the study, effects of prepartum supplementation were only evaluated within each lactation ration group. Conception rate to first service was higher (P<0.001) for B than SB supplemented cows in Group 1 and higher (P<0.02) for F than C supplemented cows in Group 2. Services per conception were lower (P=0.06) for B than SB supplemented cows in Group 1 and lower (P<0.05) for F than C supplemented cows in Group 2. Overall pregnancy rates and days open were not significantly different between the groups. The data shows that pre-partum nutrition had an important role in determining subsequent fertility. Despite having negative effects early post partum, supplementing with fat did not affect overall reproductive performance but it did improve milk production.

scholarly journals Systematic clinical examination of early postpartum cows and treatment of puerperal metritis did not have any beneficial effect on subsequent reproductive performance

2008 ◽  
Vol 53 (No. 2) ◽  
pp. 59-69 ◽  
Author(s):  
R. Dolezel ◽  
M. Vecera ◽  
T. Palenik ◽  
S. Cech ◽  
M. Vyskocil
Keyword(s):  
Beneficial Effect ◽  
Dairy Cows ◽  
Clinical Examination ◽  
Reproductive Performance ◽  
Pathological Condition ◽  
Post Partum ◽  
Control Program ◽  
Severe Form ◽  
Early Postpartum ◽  
The Impact

The objective of this field trial was to evaluate the impact of a control program based on systematic clinical examination on Day 10 ± 3<I> post partum (pp)</I> and treatment in the case of puerperal metritis on reproductive performance in dairy cows. Cows having serious dystocia as well as cows treated for retained placenta were not involved in the trial. The evaluation was performed by comparing reproductive performance between systematically examined cows (Group E, <I>n</I> = 83) and cows that were examined and treated occasionally on the basis of farmers’ notifications of a pathological condition (Group C, <I>n</I> = 95). In Group E, reproductive performance was compared between cows with a mild form of metritis (Group E1, <I>n</I> = 18), cows with a severe form of metritis (Group E2, <I>n</I> = 14) and cows without puerperal metritis (Group E0, <I>n</I> = 51). Clinical examination consisted in rectal and vaginal palpation with inspection of the lochia manually withdrawn from the vagina. Cows with puerperal metritis were treated with an intramuscular administration of PGF<sub>2&alpha;</sub> analogue – dinoprost. An intrauterine antibiotic (rifaximin foam) was added in cases of severe metritis. The examination and treatment (in cases of a pathological condition, <I>n</I> = 10) were repeated in Group E on Day 24 ± 3 <I>pp</I>. In addition, the incidence of puerperal metritis in the year seasons was evaluated. Occurrence of ovarian disorders (30.1 vs. 24.2%) and clinical endometritis (27.7 vs. 23.2%), calving to first service interval (83.2 vs. 85.4 days), pregnancy until Day 100 <I>pp</I> (30.8 vs. 35.3%) as well as until Day 150 <I>pp</I> (64.6 vs. 64.7%), services per conception (2.45 vs. 2.16), calving to conception interval (141.6 vs. 136.4 days), total culling rate (20.5 vs. 28.4%) and culling rate due to (sub)infertility (8.4 vs. 6.3) in Group E compared to Group C were not different. Only the first service pregnancy rate was lower in Group E (30.3 vs. 47.1%, <I>P</I> < 0.05). Even though no significant differences were found in the reproduction parameters between groups E1, E2 and E0, the worst parameters were in Group E2. The incidence of puerperal metritis in the year seasons was not different. The trial did not prove that there was a beneficial effect of systematic clinical examination on Day 10 ± 3 <I>pp</I> nor of treatment in cases of puerperal metritis, using PGF<sub>2&alpha;</sub> and intrauterine antibiotic, on reproductive performance in dairy cows.

scholarly journals PSII-2 The impact of essential oil supplementation on sow milk composition and fecal dry matter

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 229-229
Author(s):  
Shannon L Dierking ◽  
H J Monegue ◽  
Merlin D Lindemann
Keyword(s):  
Dry Matter ◽  
Milk Composition ◽  
Late Gestation ◽  
Gross Energy ◽  
Group 3 ◽  
Group 2 ◽  
The Individual ◽  
Dietary Treatments ◽  
The Impact ◽  
Group 1

Abstract The transition from gestation to lactation greatly impacts the sow body. The expulsion of piglets, placenta, and fluids can result in dehydration and consequently constipation post-farrowing. The objective of this study was to determine the effects of an EO product (tradename Absorbezz) on sow fecal dry matter and milk composition. A total of 101 late-gestation sows from three farrowing groups (Group 1: 110 ± 3 d GEST, 263 ± 28 kg initial BW, Group 2: 109 ± 1 d GEST, 259 ± 26 kg initial BW, Group 3: 73 ± 5 d GEST, 238 ± 30 kg initial BW) were allotted to one of two treatments based on parity and BW in each group (Groups 1 and 2: CON: No EO, TRT: 10 mL/d of EO top-dressed; Group 3 received the EO as part of their diet, with a range dependent on the individual sow’s daily feed intake). Sows remained on their respective dietary treatments throughout the remainder of gestation and lactation. Samples were collected from each group (feces; d 110 of gestation, d 4 to 6 and day 14 to 17 of lactation; milk (Group 2 and 3); d 14 to 17). Analysis of variance was performed using PROC GLM of SAS. The TRT group did not experience any differences in the components of fat, protein, and total solids (P > 0.10), there was an increase in lactose (5.97 vs 5.84, P = 0.04), but a decrease in solids non-fat (10.91 vs. 11.00, P = 0.07), and gross energy compared to those of the CON group (4.29 mJ/kg vs. 4.45 mJ/kg, P = 0.08). Fecal DM was not affected at any collection time (P > 0.10). Overall, the addition of EO did not affect fecal DM, but did influence several milk components.

Post partum suboestrus in dairy cows : comparison of treatment with prostaglandin F2α or GnRH + prostaglandin F2α + GnRH

2001 ◽  
Vol 26 (2) ◽  
pp. 347-352 ◽  
Author(s):  
B. Grimard ◽  
G. Laumonnier ◽  
C. Ponsart ◽  
H. Fauxpoint ◽  
E. Barassin ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Post Partum ◽  
Prostaglandin F2α ◽  
Specific Protein ◽  
Pregnancy Rates ◽  
Progesterone Concentration ◽  
Treatment Groups ◽  
Group 2 ◽  
Group 1

AbstractTwo studies (expt. 1, 185 cows in 1996/97; expt. 2, 168 cows in 1997/98) were conducted with Prim ‘Holstein dairy cows in the Mayenne region (France) to investigate suboestrus. Cows which had not been observed in oestrus since calving were allocated alternately to treatment groups between 60 and 90 days post partum. Expt. 1. Group 1: GnRH (Day 0, 100 μg im), PGF2α (Day 7, 25 mg im), GnRH (Day 9, 100 μg im) with artificial insemination (AI) Day 10. Group 2 : PGF2α (Day 0, 25 mg im), AI at oestrus or if oestrus not observed a second PGF2α injection was given (Day 13) with AI on Day 16 and Day 17. Expt. 2. Group 1 : GnRH (Day 0, 100 /μg im), PGF2α (Day 7, 25 mg im), GnRH (Day 9, 100 μg im) with AI at observed oestrus after Day 0 or at Day 10 if oestrus not observed. Group 2 : PGF2α (Day 0, 25 mg im), AI at oestrus, or if oestrus was not observed a second PGF2α injection was given (Day 13) and AI at observed oestrus. Progesterone was measured in serum at Day 0 and in milk at AI. Pregnancy diagnosis was performed by measuring bovine Pregnancy Specific Protein B (bPSPB) (Day 50 + 3) and confirmed by ultrasonography when the result was doubtful.In expt. 1, farmers observed 47/101 (46.9 %) of group 1 cows in oestrus, 33/91 on Day 10 and 10 before Day 10. The progesterone concentrations were compatible with oestrus in 69/86 (80%) of cows on Day 10. In group 2, 36/83 (43.4 %) of cows were inseminated after the first PGF2α injection. After the second PGF2 α, only 29/43 (67 %) of cows had a low progesterone concentration at AI. The pregnancy rates were 36.1% and 32.5 % for groups 1 and 2, respectively. In expt. 2, oestrus was observed in 31/93 (33.7 %) of group 1 cows. In group 2, 51/75 (66 %) of cows were inseminated after the first injection of PGF2α, 13/75 (17.3 %) after the second and 11/75 (14.7 %) were not seen in oestrus. Pregnancy rates were 53.7% and 53.3% in groups 1 and 2, respectively. In conclusion, it is recommended that suboestrus be treated with PGF2α followed by AI at observed oestrus when oestrus detection is good while, the use of GnRH + PGF2α + GnRH is recommended when oestrus detection is poor.

scholarly journals Reproductive behavior of crossbred dairy cows grazing an intensive silvopastoral system under tropical dry forest conditions

2017 ◽  
Vol 69 (1) ◽  
pp. 1-9
Author(s):  
E. Sierra-Montoya ◽  
R. Barahona-Rosales ◽  
Z.T. Ruiz-Cortés
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
Reproductive Behavior ◽  
Nutrient Intake ◽  
Dry Matter ◽  
Dry Forest ◽  
Ovarian Activity ◽  
Silvopastoral System ◽  
Group 2 ◽  
Group 1

ABSTRACT It is widely accepted that selection for high milk yield in dairy cows has negatively affected their reproductive performance. After calving, dairy cows experience a nutritional imbalance due to an asynchrony in the occurrence of the lactation and dry matter intake peaks. In the tropics, this situation is exacerbated due to poor quality and/ or availability of the diet. A study was carried out to describe the nutritional and reproductive behavior of two-to-four parturition crossbred cows (Gyr (Bos indicus) x Holstein (Bos taurus)) divided in two groups according to their calving to first service interval (CFSI): Group 1: ≤50 days CFSI, n=7; Group 2: ≥50 days CFSI, n=8. Animals were grazing in an intensive silvopastoral system (ISS) based on Leucaena leucocephala and Cynodon plectostachyus and also received protein, energy and mineral supplementation. Dry matter (DMI) and nutrient intake were individually estimated and correlations between reproductive and nutritional parameters were performed. Additionally, serum progesterone concentration was monitored. Group 2 had greater forage and total DMI and milk production than Group 1 (P<0.05). Hence, Group 2 had greater nutrient intake from forages than Group 1 (P<0.05). Group 1 had a positive nutritional balance and an optimum reproductive performance compared to Group 2, which had a negative energy balance and lengthier days with open intervals (P<0.05). It was corroborated that protein and energy are required in adequate levels, to ensure that uterus undergoes involution correctly and ovarian activity starts soon in the postpartum period.

scholarly journals O CLOPROSTENOL E O HORMÔNIO LIBERADOR DE GONADOTROFINAS NA PERFORMANCE REPRODUTIVA DE VACAS LEITEIRAS APÓS O PUERPÉRIO

1999 ◽  
Vol 4 (1) ◽  
Author(s):  
LUIZ ERNANDES KOZICKI ◽  
ROMILDO ROMUALDO WEISS
Keyword(s):  
Dairy Cows ◽  
Intramuscular Injection ◽  
Post Partum ◽  
Research Work ◽  
Control Group ◽  
Group 3 ◽  
Group 2 ◽  
Rectal Palpation ◽  
Group 1 ◽  
Holstein Dairy Cows

O propósito da presente pesquisa foi o de estudar a indução do estro após o período puerperal em vacas de leite Holstein. Um grupo de 50 vacas da raça Holstein, oriundas da região de Curitiba, foi utilizado neste experimento. Para tanto, esses animais foram divididos em três grupos. No 50o dia após o parto, Cloprostenol, Gonadorelina = GnRH = e soro fisiológico foram administrados por injeção intramuscular na seguinte ordem: ao grupo 1 (n = 24), 0.5 mgde Cloprostenol, ao grupo 2 (n = 14), 250 g de GnRH, e ao grupo 3 (n = 12) - grupo controle - 5 ml de soro fisiológico. Palpação retal foi levada a efeito em todos os animais no 42o/ 43o, no 50o = dia da administração das substãncias em estudo = e no 57o e no 64o dias post partum, a fim de conhecer as condições do útero e das funções ovarianas. Verificou-se que o Cloprostenol induz o estro more rapidamente do que o GnRH ou o placebo (p < 0.01). O índice de prenhez após a primeira e a segunda inseminações artificiais, quando sob a ação do Cloprostenol (84.5%) foi superior àquele que ocorreu com o GnRH (75%) ou com o placebo (67%), neste caso no grupo controle. O intervalo parto/prenhez no grupo do Cloprostenol foi menor (89 dias) doque no caso do GnRH (127.1 dias) ou do grupo controle (108.8 days). O número de animais que não responderam aos diferentes tratamentos foi menor no caso do grupo do Cloprostenol. Por outro lado o tratamento com GnRH mostrou-se mais eficiente (p < 0.05) do que o grupo controle com respeito à indução ao estro (18.6 e 43.5 dias, respectivamente). Os grupos do GnRH e do Cloprostenol revelaram índices maiores de estro silencioso (33.3 e 28.5%, respectivamente), diferente do que ocorreu com o grupo controle (16.6 %) (p < 0.05). Abstract The purpose of the present research work was to study estrus’ induction after the puerperal period in Holstein dairy cows. A herd of 50 Holstein dairy cows from the Curitiba’s region were used in this experiment. They were divided in three groups. At the 50th day post partum, Cloprostenol, Gonadorelin = GnRH = and saline solution were administered by intramuscular injection to group 1 (n = 24), 0.5 mg of Cloprostenol, to group 2 (n = 14), 250 g of GnRH, and to group 3 (n = 12), 5 ml of saline as placebo. Rectal palpation was performed in all animals on the 42/43rd, the 50th = the day of the intramuscular injection = and on the 57th and 64th day post partum, in order to know the uterus condition and the ovary’s functions. It has been found that Cloprostenol induces cow’s estrus more quickly than GnRH or the placebo (p < 0.01). The rate of pregnancy after the 1st and the 2nd artificial insemination, under Cloprostenol effect (84.5%) was greater than under GnRH (75%) or the placebo (67%) at the control group. The parturition/pregnancy interval in the Cloprostenol group was smaller (89 days) than in the GnRH (127.1 days) or in the control (108.8 days) groups. The number of animals that did not respond to the treatment was smaller in the Cloprostenol group. On the other hand, the GnRH group was more efficient (p < 0.05) than the control group on the estrus’ induction (18.6 versus 43.5 days, respectively). The GnRH and the Cloprostenol groups displayed higher rates of silent estrus (33.3 and 28.5%, respectively) than the control group (16.6 %) (p < 0.05).

CD34 immunostain increases sensitivity of the diagnosis of fetal vascular malperfusion in placentas from ex-utero intrapartum treatment

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jerzy Stanek
Keyword(s):  
Statistical Significance ◽  
Treatment Procedure ◽  
Fetal Survival ◽  
Ex Utero Intrapartum Treatment ◽  
Life Threatening ◽  
Group 2 ◽  
Cd34 Immunostain ◽  
The Impact ◽  
Diaphragmatic Hernias ◽  
Group 1

AbstractShort CommunicationsEXIT (ex-utero intrapartum treatment) procedure is a fetal survival-increasing modification of cesarean section. Previously we found an increase incidence of fetal vascular malperfusion (FVM) in placentas from EXIT procedures which indicates the underlying stasis of fetal blood flow in such cases. This retrospective analysis analyzes the impact of the recently introduced CD34 immunostain for the FVM diagnosis in placentas from EXIT procedures.Objectives and MethodsA total of 105 placentas from EXIT procedures (48 to airway, 43 to ECMO and 14 to resection) were studied. In 73 older cases, the placental histological diagnosis of segmental FVM was made on H&E stained placental sections only (segmental villous avascularity) (Group 1), while in 32 most recent cases, the CD34 component of a double E-cadherin/CD34 immunostain slides was also routinely used to detect the early FVM (endothelial fragmentation, villous hypovascularity) (Group 2). 23 clinical and 47 independent placental phenotypes were compared by χ2 or ANOVA, where appropriate.ResultsThere was no statistical significance between the groups in rates of segmental villous avascularity (29 vs. 34%), but performing CD34 immunostain resulted in adding and/or upgrading 12 more cases of segmental FVM in Group 2, thus increasing the sensitivity of placental examination for FVM by 37%. There were no other statistically significantly differences in clinical (except for congenital diaphragmatic hernias statistically significantly more common in Group 2, 34 vs 56%, p=0.03) and placental phenotypes, proving the otherwise comparability of the groups.ConclusionsThe use of CD34 immunostain increases the sensitivity of placental examination for FVM by 1/3, which may improve the neonatal management by revealing the increased likelihood of the potentially life-threatening neonatal complications.

scholarly journals The negative effects of COVID-19 and national lockdown on emergency surgery morbidity due to delayed access

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Francesco A. Ciarleglio ◽  
Marta Rigoni ◽  
Liliana Mereu ◽  
Cai Tommaso ◽  
Alessandro Carrara ◽  
...  
Keyword(s):  
Emergency Department ◽  
Blood Transfusion ◽  
Emergency Surgery ◽  
Surgical Care ◽  
Close Correlation ◽  
Negative Effects ◽  
Group 2 ◽  
Retrospective Comparative Study ◽  
The Impact ◽  
Group 1

Abstract Background The aim of this retrospective comparative study was to assess the impact of COVID-19 and delayed emergency department access on emergency surgery outcomes, by comparing the main clinical outcomes in the period March–May 2019 (group 1) with the same period during the national COVID-19 lockdown in Italy (March–May 2020, group 2). Methods A comparison (groups 1 versus 2) and subgroup analysis were performed between patients’ demographic, medical history, surgical, clinical and management characteristics. Results Two-hundred forty-six patients were included, 137 in group 1 and 109 in group 2 (p = 0.03). No significant differences were observed in the peri-operative characteristics of the two groups. A declared delay in access to hospital and preoperative SARS-CoV-2 infection rates were 15.5% and 5.8%, respectively in group 2. The overall morbidity (OR = 2.22, 95% CI 1.08–4.55, p = 0.03) and 30-day mortality (OR = 1.34, 95% CI 0.33–5.50, =0.68) were significantly higher in group 2. The delayed access cohort showed a close correlation with increased morbidity (OR = 3.19, 95% CI 0.89–11.44, p = 0.07), blood transfusion (OR = 5.13, 95% CI 1.05–25.15, p = 0.04) and 30-day mortality risk (OR = 8.00, 95% CI 1.01–63.23, p = 0.05). SARS-CoV-2-positive patients had higher risk of blood transfusion (20% vs 7.8%, p = 0.37) and ICU admissions (20% vs 2.6%, p = 0.17) and a longer median LOS (9 days vs 4 days, p = 0.11). Conclusions This article provides enhanced understanding of the effects of the COVID-19 pandemic on patient access to emergency surgical care. Our findings suggest that COVID-19 changed the quality of surgical care with poorer prognosis and higher morbidity rates. Delayed emergency department access and a “filter effect” induced by a fear of COVID-19 infection in the population resulted in only the most severe cases reaching the emergency department in time.

scholarly journals QOL-49. THE IMPACT OF OTOTOXICITY AND VISUAL IMPAIRMENT ON EDUCATION IN CHILDREN TREATED FOR CNS TUMOURS

2020 ◽  
Vol 22 (Supplement_3) ◽  
pp. iii440-iii440
Author(s):  
Harriet Dulson ◽  
Rachel McAndrew ◽  
Mark Brougham
Keyword(s):  
Visual Impairment ◽  
Impaired Hearing ◽  
Cranial Radiotherapy ◽  
Radiotherapy Group ◽  
Platinum Based Chemotherapy ◽  
Cns Tumours ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

Abstract INTRODUCTION Children treated for CNS tumours experience a very high burden of adverse effects. Platinum-based chemotherapy and cranial radiotherapy can cause ototoxicity, which may be particularly problematic in patients who have impaired vision and cognition as a result of their tumour and associated treatment. This study assessed the prevalence of impaired hearing and vision and how this may impact upon education. METHODS 53 patients diagnosed with solid tumours in Edinburgh, UK between August 2013–2018 were included in the study. Patients were split into three groups according to treatment received: Group 1 – cisplatin-based chemotherapy and cranial radiotherapy; Group 2 - platinum-based chemotherapy, no cranial radiotherapy; Group 3 – benign brain tumours treated with surgery only. Data was collected retrospectively from patient notes. RESULTS Overall 69.5% of those treated with platinum-based chemotherapy experienced ototoxicity as assessed by Brock grading and 5.9% of patients had reduced visual acuity. Patients in Group 1 had the highest prevalence of both. 44.4% of patients in Group 1 needed increased educational support following treatment, either with extra support in the classroom or being unable to continue in mainstream school. 12.5% of Group 2 patients required such support and 31.3% in Group 3. CONCLUSIONS Children with CNS tumours frequently require support for future education but those treated with both platinum-based chemotherapy and cranial radiotherapy are at particular risk, which may be compounded by co-existent ototoxicity and visual impairment. It is essential to provide appropriate support for this patient cohort in order to maximise their educational potential.

scholarly journals Association between serum oestradiol level on the hCG administration day and neonatal birthweight after IVF-ET among 3659 singleton live births

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu Liu ◽  
Jing Li ◽  
Wanyu Zhang ◽  
Yihong Guo
Keyword(s):  
Birth Weight ◽  
Ovarian Stimulation ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
The Impact ◽  
Ivf Et ◽  
Group 1

AbstractOestradiol, an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization-embryo transfer (IVF-ET) cycles. A supraphysiologic E2 level is inevitable during controlled ovarian hyper-stimulation (COH), and its effect on the outcome of IVF-ET is controversial. The aim of this retrospective study is to evaluate the association between elevated serum oestradiol (E2) levels on the day of human chorionic gonadotrophin (hCG) administration and neonatal birthweight after IVF-ET cycles. The data of 3659 infertile patients with fresh IVF-ET cycles were analysed retrospectively between August 2009 and February 2017 in First Hospital of Zhengzhou University. Patients were categorized by serum E2 levels on the day of hCG administration into six groups: group 1 (serum E2 levels ≤ 1000 pg/mL, n = 230), group 2 (serum E2 levels between 1001 and 2000 pg/mL, n = 524), group 3 (serum E2 levels between 2001 and 3000 pg/mL, n = 783), group 4 (serum E2 levels between 3001 and 4000 pg/mL, n = 721), group 5 (serum E2 levels between 4001 and 5000 pg/mL, n = 548 ), and group 6 (serum E2 levels > 5000 pg/mL, n = 852). Univariate linear regression was used to evaluate the independent correlation between each factor and outcome index. Multiple logistic regression was used to adjust for confounding factors. The LBW rates were as follows: 3.0% (group 1), 2.9% (group 2), 1.9% (group 3), 2.9% (group 4), 2.9% (group 5), and 2.0% (group 6) (P = 0.629), respectively. There were no statistically significant differences in the incidences of neonatal LBW among the six groups. We did not detect an association between peak serum E2 level during ovarian stimulation and neonatal birthweight after IVF-ET. The results of this retrospective cohort study showed that serum E2 peak levels during ovarian stimulation were not associated with birth weight during IVF cycles. In addition, no association was found between higher E2 levels and increased LBW risk. Our observations suggest that the hyper-oestrogenic milieu during COS does not seem to have adverse effects on the birthweight of offspring after IVF. Although this study provides some reference, the obstetric-related factors were not included due to historical reasons. The impact of the high estrogen environment during COS on the birth weight of IVF offspring still needs future research.

scholarly journals THU0227 CAFFEINE INTAKE MODULATES DISEASE ACTIVITY AND CYTOKINES LEVELS IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 340.2-341
Author(s):  
V. Orefice ◽  
F. Ceccarelli ◽  
C. Barbati ◽  
R. Lucchetti ◽  
G. Olivieri ◽  
...  
Keyword(s):  
Disease Activity ◽  
Lupus Erythematosus ◽  
Caffeine Intake ◽  
Caffeine Consumption ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

Background:Systemic lupus erythematosus (SLE) is an autoimmune disease mainly affecting women of childbearing age. The interplay between genetic and environmental factors may contribute to disease pathogenesis1. At today, no robust data are available about the possible contribute of diet in SLE. Caffeine, one of the most widely consumed products in the world, seems to interact with multiple components of the immune system by acting as a non-specific phosphodiesterase inhibitor2.In vitrodose-dependent treatment with caffeine seems to down-regulate mRNA levels of key inflammation-related genes and similarly reduce levels of different pro-inflammatory cytokines3.Objectives:We evaluated the impact of caffeine consumption on SLE-related disease phenotype and activity, in terms of clinimetric assessment and cytokines levels.Methods:We performed a cross-sectional study, enrolling consecutive patients and reporting their clinical and laboratory data. Disease activity was assessed by SLE Disease Activity Index 2000 (SLEDAI-2k)4. Caffeine intake was evaluated by a 7-day food frequency questionnaire, including all the main sources of caffeine. As previously reported, patients were divided in four groups according to the daily caffeine intake: <29.1 mg/day (group 1), 29.2-153.7 mg/day (group 2), 153.8-376.5 mg/day (group 3) and >376.6 mg/day (group 4)5. At the end of questionnaire filling, blood samples were collected from each patient to assess cytokines levels. These were assessed by using a panel by Bio-Plex assays to measure the levels of IL-6, IL-10, IL-17, IL-27, IFN-γ, IFN-α and Blys.Results:We enrolled 89 SLE patients (F/M 87/2, median age 46 years, IQR 14; median disease duration 144 months, IQR 150). The median intake of caffeine was 195 mg/day (IQR 160.5). At the time of the enrollment, 8 patients (8.9%) referred a caffeine intake < 29.1 mg/day (group 1), 27 patients (30.3%) between 29.2 and 153.7 mg/day (group 2), 45 patients (51%) between 153.8 and 376.5 mg/day (group 3) and 9 patients (10.1%) >376.6 mg/day (group 4). A negative correlation between the levels of caffeine and disease activity, evaluated with SLEDAI-2K, was observed (p=0.01, r=-0.26). By comparing the four groups, a significant higher prevalence of lupus nephritis, neuropsychiatric involvement, haematological manifestations, hypocomplementemia and anti-dsDNA positivity was observed in patients with less intake of caffeine (figure 1 A-E). Furthermore, patients with less intake of caffeine showed a significant more frequent use of glucocorticoids [group 4: 22.2%,versusgroup 1 (50.0%, p=0.0001), group 2 (55.5%, p=0.0001), group 3 (40.0%, p=0.009)]. Moving on cytokines analysis, a negative correlation between daily caffeine consumption and serum level of IFNγ was found (p=0.03, r=-0.2) (figure 2A); furthermore, patients with more caffeine intake showed significant lower levels of IFNα (p=0.02, figure 2B), IL-17 (p=0.01, figure 2C) and IL-6 (p=0.003, figure 2D).Conclusion:This is the first report demonstrating the impact of caffeine on SLE disease activity status, as demonstrated by the inverse correlation between its intake and both SLEDAI-2k values and cytokines levels. Moreover, in our cohort, patients with less caffeine consumption seems to have a more severe disease phenotype, especially in terms of renal and neuropsychiatric involvement. Our results seem to suggest a possible immunoregulatory dose-dependent effect of caffeine, through the modulation of serum cytokine levels, as already suggested byin vitroanalysis.References:[1]Kaul et alNat. Rev. Dis. Prim.2016; 2. Aronsen et alEurop Joul of Pharm2014; 3. Iris et alClin Immun.2018; 4. Gladman et al J Rheumatol. 2002; 5. Mikuls et alArth Rheum2002Disclosure of Interests:Valeria Orefice: None declared, Fulvia Ceccarelli: None declared, cristiana barbati: None declared, Ramona Lucchetti: None declared, Giulio Olivieri: None declared, enrica cipriano: None declared, Francesco Natalucci: None declared, Carlo Perricone: None declared, Francesca Romana Spinelli Grant/research support from: Pfizer, Consultant of: Novartis, Gilead, Lilly, Sanofi, Celgene, Speakers bureau: Lilly, cristiano alessandri Grant/research support from: Pfizer, Guido Valesini: None declared, Fabrizio Conti Speakers bureau: BMS, Lilly, Abbvie, Pfizer, Sanofi

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