Fractographic Studies of the Honey Bee Sensilla Coeloconica and Sensilla Amupullacea Campaniformia by SEM

1974 ◽  
Vol 32 ◽  
pp. 186-187
Author(s):  
Alfred Dietz ◽  
Leroy M. Anderson ◽  
Malcolm T. Sanford
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Surface Structure ◽  
Honey Bee ◽  
Honey Bees ◽  
Sense Organs ◽  
The Past ◽  
Sensory Structures ◽  
Scanning Electron

The antennal sensory organs of honey bees have been studied by many researchers in the past. In most instances their work was confined to readily identifiable cuticular sensory structures such as the pore plate organ (Figs. 1, 3) and several types of hair-like sensilla (Fig. 1). The total number of receptors on a honey bee antennae is roughly 13,000 of which about 8,200 belong to the hair-like receptors or s. trichodea group (Fig. 1). The pore plate organs or s. placodea comprise the next largest group with 3,000 receptors. The pit peg sense organs or s. ampullacea (Figs. 3, 5), and s. coeloconica (Figs. 1, 2) are present in considerably smaller number (approximately 300) and have received little attention since they cannot be readily identified on the basis of their surface structure. Thus, little is known about the fine structure of these pit peg organs. In this study, pit peg organs of plastic embedded antennae were examined by scanning electron microscopy.

scholarly journals Scanning electron microscopic studies on tongue of open-nesting honey bees Apis dorsata F. and Apis florea F. (Hymenoptera: Apidae)

2015 ◽  
Vol 7 (1) ◽  
pp. 324-327
Author(s):  
Neelima R. Kumar ◽  
Kalpna Nayyar ◽  
Ruchi Sharma ◽  
Anudeep Anudeep
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Honey Bee ◽  
Honey Bees ◽  
Floral Resources ◽  
Apis Florea ◽  
Apis Dorsata ◽  
Electron Microscopic ◽  
Native Flora ◽  
Scanning Electron

Taste stimuli play vital role in the life of honey bees. Sensory structures observed on tongue of the honey bees with the help of Scanning electron microscopy (SEM) have become an important tool in analyzing honey bee biodiversity which offers an advanced diagnostic tool to study honey bee biogeography and determine adaptive variations to native flora. Tongue of honey bees present a high geographic variability in regard to the floral resources visited by the bees. The present study has determined to determine differences in the tongue ofopen-nesting bees by scanning electron microscopy of Apis dorsata and Apis florea. The two bees showed distinct morphological variations with respect to the lapping and sucking apparatus. It was observed that the ridges on the proximal region exhibited rough surface on A.dorsata whereas spinous in case of A.florea. Moreover, the arrangement of hair in the middle part of the tongue also differed in the two species. The shape of flabellum differed in the two species reason being the influence of native flora. It was observed that the shape of flabellum was oval in A.dorsata whereas in A.florea it was triangular. These differences indicated for the role of native flora and honey bee biodiversity.

SEM observations on egg-shell of the Japanese tusser, Antheraea yamamai and Chinese tusser,Antheraea pernyi

1990 ◽  
Vol 48 (3) ◽  
pp. 98-99
Author(s):  
Hu Cui ◽  
Jian Hong ◽  
Gao Oikang ◽  
Wu Xiaojiang ◽  
Ye Gongyin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Surface Structure ◽  
Shell Surface ◽  
Antheraea Pernyi ◽  
Egg Shells ◽  
Egg Shell ◽  
Scanning Electron

The egg-shell surface structure of the Japanese tusser and Chinese tusser was observed by means of scanning electron microscopy. There were a lot of similarities between the two egg-shells, but the fine structure may be easily distinguished. As to the Japanese tusser, the petals of the petaloid pattern around micropyle were elongate and raised in the middle (Fig. 1); micropylar tubes numbered 11-13 (Figs. 2 and 3); the wider and thicker bank formed irregularly shaped net-like structure of the egg-shell surface other than in the vicinity of micropyle (Fig. 5); and the thickness of the egg-shell was about 70 μm. In the Chinese tusser the petals were shorter, wider,and even; micropylar tubes numbered 8-9 (Fig. 4); the narrow and low bank formed hexagonal, pentagonal, heptagonal, or octagonal net-like structure; the aeropyle wall was well developed, almost the same in size (Figs. 8 and 9); and the thickness of the egg-shell was about 40 μm.

scholarly journals Differences in the number of antennal sensory structures of males of three honey bee types

1999 ◽  
Vol 59 (1) ◽  
pp. 161-166 ◽  
Author(s):  
A. C. STORT ◽  
M. M. B. MORAES-ALVES
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Honey Bee ◽  
The Other ◽  
Sensory Structures ◽  
Scanning Electron

The number of sensilla campaniformia and sensilla coeloconica + sensilla ampullacea of flagellomeres 2 to 11 of the antennae of three types of males (Italian, African and Africanized) was determined by scanning electron microscopy. Comparison of the three male types showed that Italian males did not differ from African males in number of sensilla coeloconica + sensilla ampullacea and that both differed from Africanized males in terms of flagellomere 11. With respect to flagellomeres 3 and 10, Italian males were similar to Africanized males and both differed from African males. No differences between the three male types were detected in the other flagellomeres. In relation to the number of sensilla campaniformia Italian males differed of the African and Africanized males with respect to flagellomere 11.

Labrocibarial sensilla in the female of the black fly Simulium damnosum s.l. (Diptera: Simuliidae)

1987 ◽  
Vol 65 (2) ◽  
pp. 441-444 ◽  
Author(s):  
D. Jefferies
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
External Morphology ◽  
Sense Organs ◽  
Black Fly ◽  
Food Canal ◽  
Simulium Damnosum ◽  
Scanning Electron

Five types of sensilla were identified at the entrance to or within the food canal in female Simulium damnosum s.l., four on the labrum and one in the cibarium. The external morphology of these sense organs was investigated using scanning electron microscopy. Other cuticular projections present do not appear to be sensory in nature. No differences were found in the number, position, or fine structure of the mouthpart sensilla in two different cytospecies of S. damnosum, S. sanctipauli Vajime &Dunbar and S. sirbanum Vajime &Dunbar.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

Comparative Study of the Fine Morphology of Sensory Receptors in Aspidogaster conchicola and Cotylaspis insignis

1972 ◽  
Vol 30 ◽  
pp. 150-151
Author(s):  
Venita F. Allison ◽  
J. E. Ubelaker ◽  
J. H. Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nervous System ◽  
Osmic Acid ◽  
Sensory Receptors ◽  
Transmission Electron ◽  
Sensory Structures ◽  
Fine Morphology ◽  
Scanning Electron

It has been suggested that parasitism results in a reduction of sensory structures which concomitantly reflects a reduction in the complexity of the nervous system. The present study tests this hypothesis by examining the fine morphology and the distribution of sensory receptors for two species of aspidogastrid trematodes by transmission and scanning electron microscopy. The species chosen are an ectoparasite, Cotylaspis insignis and an endoparasite, Aspidogaster conchicola.Aspidogaster conchicola and Cotylaspis insignis were obtained from natural infections of clams, Anodonta corpulenta and Proptera purpurata. The specimens were fixed for transmission electron microscopy in phosphate buffered paraformaldehyde followed by osmic acid in the same buffer, dehydrated in an ascending series of ethanol solutions and embedded in Epon 812.

Ultrastructure of the soil fungus, Geomyces pannorus

1984 ◽  
Vol 42 ◽  
pp. 738-739
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Low Temperatures ◽  
Soil Fungus ◽  
Organic Soils ◽  
Anatomical Studies ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

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