Tubular intranuclear inclusions in a patient with methotrexate-associated pulmonary fibrosis

1983 ◽  
Vol 41 ◽  
pp. 796-797
Author(s):  
W.T. Gunning ◽  
P.J. Goldblatt
Keyword(s):  
Interstitial Fibrosis ◽  
Inflammatory Cells ◽  
Giant Cells ◽  
Skin Condition ◽  
Intermittent Fever ◽  
Electron Microscopic ◽  
Type Ii Pneumocytes ◽  
Microscopic Evaluation ◽  
Microscopic Studies ◽  
History Of

A forty-six year old male, who had received methotrexate therapy for the past year for psoriasis of twenty years' duration, presented with a cough which had persisted for two to three months. There was a two year history of dyspnea upon exertion as well as intermittent fever, chills and sweats. The methotrexate treatment did not improve the skin condition and, because there was evidence of hepatic toxicity, the drug was discontinued. Radiological examination of the chest revealed diffuse bilateral interstitial fibrosis with extensive changes; a subseguent lung biopsy was obtained. Light microscopic evaluation demonstrated marked interstitial fibrosis, chronic inflammation, thickening of the alveolar septa, proliferation of alveolar macrophages, occasional fibrin deposits and multinucleated giant cells.Electron microscopic studies revealed massive fibrosis with a mixed population of infiltrating inflammatory cells. Some alveolar spaces were lined by metaplastic cuboidal epithelial cells, and in numerous residual type II pneumocytes, aggregates of branching tubules lined by trilaminar membranes were identified within the nucleus.

scholarly journals Equine multinodular pulmonary fibrosis in southern Brazil: pathology and differential diagnosis

2017 ◽  
Vol 37 (11) ◽  
pp. 1247-1252 ◽  
Author(s):  
Pablo Estima-Silva ◽  
Clairton Marcolongo-Pereira ◽  
Bianca Lemos dos Santos ◽  
Ana Carolina Barreto Coelho ◽  
Lorena Alvariza Amaral ◽  
...  
Keyword(s):  
Pulmonary Fibrosis ◽  
Interstitial Fibrosis ◽  
Clinical Signs ◽  
Giant Cells ◽  
Interstitial Tissue ◽  
Intermittent Fever ◽  
Type Ii Pneumocytes ◽  
Sporadic Disease ◽  
De Medicina

ABSTRACT: Equine multinodular pulmonary fibrosis (EMPF) diagnosed at the Laboratório Regional de Diagnóstico of Faculdade de Medicina Veterinária, Universidade Federal de Pelotas (LRD/UFPel), is described. Differential aspects of other pulmonary diseases in horses with pneumonia and interstitial fibrosis were discussed. The disease occurred in a 15-year-old equine that presented with clinical signs of respiratory distress, intermittent fever, anorexia, and dyspnea. Macroscopically, there was enlargement of the lungs with whitish, pale, firm and well-delimited nodules, approximately 7-10 cm in diameter, distributed throughout the parenchyma. Histologically, the lung nodules had alveolar spaces with walls covered by cuboidal epithelium containing macrophages, neutrophils, lymphocytes, hyperplasia of type II pneumocytes and, eventually, multinucleated giant cells. The interstitium was markedly thickened by mature fibrous connective tissue and collagen. There were intranuclear inclusion bodies in the macrophages. The PCR technique for detecting the EHV-5 DNA was positive. In a retrospective study of pneumonia cases in horses with interstitial fibrosis diagnosed in the LRD/UFPel, two animals had macroscopic and histological lesions similar to those with EMPF, but they were negative for EHV-5 in PCR. Four cases diagnosed with pneumonia and interstitial tissue fibrosis had a histological pattern that was different from that observed in the EMPF animal, thus eliminating the possibility of EMPF. It is concluded that EMPF is a sporadic disease that should be considered in cases of respiratory disease in horses. Reports of such cases are important to alert technicians about the occurrence of rare diseases in Brazil. It is also necessary to establish the true role of EHV-5 in the pathogenesis of EMPF. Cases of pulmonary fibrosis such as EMPF, in which the virus is not present, should be studied to establish whether it could be an idiopathic form of the disease.

Filiform polyposis in a 20-year-old patient with ulcerative colitis – a rare occurrence

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S60-S60
Author(s):  
R Ramanarasimhaiah ◽  
B Chae ◽  
M Toprak ◽  
L M Opitz ◽  
H Wu
Keyword(s):  
Ulcerative Colitis ◽  
Surgical Margin ◽  
Inflammatory Cells ◽  
Clinical History ◽  
Right Colon ◽  
Microscopic Evaluation ◽  
Bowel Diseases ◽  
History Of ◽  
Filiform Polyposis ◽  
The Right

Abstract Introduction/Objective Filiform polyposis (FP), also referred as inflammatory polyposis or pseudopolyposis, is an uncommon benign entity usually diagnosed in patients with history of Crohn’s disease, ulcerative colitis, interstitial tuberculosis or histiocytosis X. It is characterized by multiple finger-like mucosal projections/pseudopolyps seen more commonly in transverse and descending colon. Pathogenesis of FP is uncertain but it is hypothesized to be reactive process to chronic inflammation leading to formation of fingerlike projections. The term filiform polyposis was coined in 1974 by Appleman HD and his collegues to describe numerous long slender worm-like (filiform) colonic lesions seen in radiographs. Herein, we are going to present a case of FP diagnosed in a 20-year-old male with history of medically refractive ulcerative colitis. Methods/Case Report The patient is a 20-year-old male, with a history of medically refractive ulcerative colitis on systemic steroids and vedolizumab. He underwent sigmoidoscopy and colonoscopy which reveals multiple pedunculated, polypoid lesions, ranging from 0.5 to 3 cm in length with white tips (cap of purulent mucous). Patient underwent total abdominal colectomy with end ileostomy. The specimen received by our deparment consist of a segment of right colon (63 cm in length, 3.5 cm in diameter) with attached ileum (3 cm in length, 2 cm in diameter) and appendix (6 cm in length, 0.8 cm in diameter). The part of the right colon is constricted/strictured measuring 24 cm in length, 2 cm in diameter. Opening reveals numerous (>100) pedunculated, finger-like polypoid lesions measuring from 0.3 cm to 2 cm in length. The lesions involve the entire right colon including distal colonic surgical margin. Appendix and ileum are not involved by the lesion and grossly unremarkable. Microscopic evaluation of the lesions reveal acute and chronic inflammatory cells, granulation tissue, areas of necrosis and reactive changes with focal mucosal atrophy consistent with chronic ulcerative colitis. Results (if a Case Study enter NA) NA Conclusion Filiform polyposis (FP) is an uncommon benign entity commonly seen in association with Inflammatory Bowel Diseases. Diagnosing FP requires comprehensive gross and microscopic examination as well as review of clinical history and imaginig studies. Because of its uncommon nature, we recommend to report this entity to raise awareness and to educate the pathologists who have not diagnosed it before.

scholarly journals Pollen morphology of selected taxa of Ehretiaceae from Western Ghats, India.

2018 ◽  
Vol 7 (11) ◽  
pp. 2446
Author(s):  
Amalaurpava Mary Michael ◽  
Vijay Gopal Gumma
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Western Ghat ◽  
Electron Microscopic ◽  
History Of Vegetation ◽  
Microscopic Studies ◽  
Reserve Forest ◽  
History Of ◽  
Phylogenetic Origin ◽  
Scanning Electron

Pollen Morphology is an important tool in the identification of a genera. Data on pollen morphology is used as a reference in other fields of palynology like allergic studies, melissopalynology, tracing the history of vegetation, genetic and evolutionary studies, climate change studies etc. Pollen morphology of two genera of Ehretiaceae family is studied using Scanning electron microscope. Palynological contributions are still fragmentary in the family Ehretiaceae of southern India. Ehretia pubescens Benth is a small tree belonging to Ehretiaceae family located at the foothill of Chamundi hill reserve forest which is a part of Western ghat near Mysuru and Cormona retusa (Vahl) Masam is a shrub found 3400ft above on top of Chamundi Hill. Pollen grains were acetolyzed and Scanning Electron Microscopic studies conducted to obtain data on pollen morphology. The study is conducted to assess the taxonomic significance of pollen morphology in relation to their phylogenetic origin of the two genera of Ehretiaceae in the region. The palynological evidence shows Ehretia pubescens with tri-zonocolporate and heterocolpate pollen grains and Cormona retusa with tricolpate pollen grain without the pseudocolpi. Both genera have foveolate tectum. Palynological data indicate that these two naturalized taxa ie. Ehretia pubescens and Cormona retusa belong to two different lineage of Ehretia and can be identified by their pollen morphology. Diversity in the pollen characters points towards the eurypalynous nature in Ehretiaceae.

scholarly journals Micropipette aspiration of human blood platelets: a defect in Bernard- Soulier's syndrome

Blood ◽  
1984 ◽  
Vol 63 (5) ◽  
pp. 1249-1252 ◽  
Author(s):  
JG White ◽  
SM Burris ◽  
D Hasegawa ◽  
M Johnson
Keyword(s):  
Blood Platelets ◽  
Surface Membrane ◽  
Giant Cells ◽  
Micropipette Aspiration ◽  
Electron Microscopic ◽  
Normal Cells ◽  
Glass Slides ◽  
Resistance To Deformation ◽  
Microscopic Studies ◽  
Cell Sizing

Abstract Previous reports have suggested that platelets from patients with Bernard-Soulier's syndrome (BSS) are not giant cells. Rather, they are normal-sized in suspension, but spread out on glass slides more readily than control cells, yielding the impression of being giant. The present study has used cell sizing techniques, electron microscopy, and micropipette aspiration to evaluate platelets from three patients with BSS. Cell sizing techniques revealed that BSS platelets were considerably larger than normal. The increased size was confirmed in electron microscopic studies of BSS platelets fixed in suspension. However, the BSS platelets did not contain increased amounts of internalized surface membrane considered to be the source of membrane necessary for excessive spreading. A possible explanation for increased spreading of BSS platelets was found in studies of their resistance to deformation in micropipettes. BSS platelets were much less resistant to deformation than normal cells or other abnormal platelets when aspirated under the same negative pressure. Their unusual deformability may explain the tendency of BSS platelets to spread more readily than normal cells on glass slides.

Morphologic Study of Carcinoid-Like Tumors and Their Relation to True Carcinoids, Using Tumors of the Breast as a Model

1983 ◽  
Vol 69 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Faripour A. Forouhar
Keyword(s):  
Ductal Carcinoma ◽  
Secretory Granules ◽  
Lobular Carcinoma ◽  
Prognostic Significance ◽  
Electron Microscopic Examination ◽  
Carcinoid Tumors ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Microscopic Studies ◽  
Membrane Bound

Two cases of an unusual type of infiltrating ductal carcinoma of the breast are presented. Both cases demonstrated a carcinoid-like pattern and were indistinguishable from carcinoid tumors of the breast by light microscopy. However, Grimelius stains and electron microscopic evaluation showed no evidence of membrane bound secretory granules. In regard to the prognostic significance and proper classification of carcinoids of the breast, awareness of carcinoid-like morphologic variants of infiltrating ductal or lobular carcinoma is important. It is also apparent that there is a spectrum of tumors which demonstrate some properties of true carcinoids, however, only the true carcinoids show a better prognosis and it serves no useful purpose to separate the rest of these tumors. The diagnosis of carcinoid tumors requires demonstration of secretory granules on electron microscopic examination or in special stains; conventional light microscopic studies alone are insufficient for this diagnosis. All these principles may be applied to carcinoid like tumors of other sites.

Tuberculoma of the Conus Medullaris: Case Report

Neurosurgery ◽  
2002 ◽  
Vol 50 (3) ◽  
pp. 651-653 ◽  
Author(s):  
Kudret Türeyen
Keyword(s):  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Mass Effect ◽  
Giant Cells ◽  
Conus Medullaris ◽  
Antituberculosis Therapy ◽  
Patient Reported ◽  
History Of ◽  
Leg Weakness ◽  
Muscle Groups

Abstract OBJECTIVE AND IMPORTANCE: Intramedullary spinal tuberculoma is a rare form of central nervous system tuberculosis. This article describes an affected patient who presented with left leg paresis. CLINICAL PRESENTATION: A 46-year-old man presented with a 7-day history of left leg weakness. The patient's medical history included infection with pulmonary tuberculosis 15 years previously, at which time he had been treated with antituberculosis therapy. The neurological examination performed at admission revealed left leg paresis with Grade 2/5 power in all muscle groups. The patient reported no urinary or bowel problems. INTERVENTION: Surgery was performed with the patient in the prone position. The procedure involved laminectomies at T11, T12, and L1, followed by a midline myelotomy. The mass was excised completely. Histopathological examination revealed a granulomatous lesion that contained Langhans' giant cells, inflammatory cells, and evidence of caseating necrosis. The patient was prescribed a 6-month course of antituberculosis therapy with pyrazinamide, isoniazid, and rifampin. CONCLUSION: The outcome was favorable. Recently, a number of authors have reported success with medical management of intraspinal tuberculoma. Intraspinal tuberculoma produces a mass effect that can jeopardize spinal cord function. The optimal treatment is a combination of microsurgical resection and antituberculosis chemotherapy.

Effect of formaldehyde inhalation on rat livers: A light and electron microscopic study

2010 ◽  
Vol 26 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Selman Cikmaz ◽  
Tunc Kutoglu ◽  
Mehmet Kanter ◽  
Recep Mesut
Keyword(s):  
Liver Tissue ◽  
Exposure Period ◽  
Electron Microscopic Level ◽  
Albino Rats ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Microscopic Evaluation ◽  
Microscopic Studies ◽  
Wistar Albino Rats ◽  
The Individual

It is well known that formaldehyde (FA) is cytotoxic and potentially carcinogenic. Although the individual effects of this reactant on cells has been investigated, the cytotoxicity exerted by the coexistence of FA is poorly understood. The aim of this study was to investigate the effects of FA on the liver in rats, by light and electron microscopic level. We used 18 Wistar albino rats divided into three groups, exposed to 0 (control), 19.7 ppm FA gas for a total of 4 weeks, 8 h/day, 5 days a week (subacute) and 20.3 parts per million (ppm) FA gas for a total of 13 weeks, 8 h/day, 5 days a week (subchronic). After the completion of the exposure period, they were sacrificed by decapitation and their liver tissue samples were taken in order to be processed for light and electron microscopic studies. Light microscopic evaluation of liver tissue samples of FA-exposed rats revealed enlarged sinusoids filled with blood and mononuclear cell infiltration in the portal areas and around the central veins. In addition, some of the hepatocytes showed loss of cytoplasm, and some had a hyperchromatic nucleus. The cells of FA-exposed livers, on the other hand, showed an electron-lucent ground-cytoplasm and a hypertrophy of the smooth-surfaced endoplasmic reticulum. In conclusion, we observed that exposure FA caused diverse histopathological changes indicating the destruction in the liver tissue and this destruction has direct relationship with the length of the exposure period.

scholarly journals Micropipette aspiration of human blood platelets: a defect in Bernard- Soulier's syndrome

Blood ◽  
1984 ◽  
Vol 63 (5) ◽  
pp. 1249-1252 ◽  
Author(s):  
JG White ◽  
SM Burris ◽  
D Hasegawa ◽  
M Johnson
Keyword(s):  
Blood Platelets ◽  
Surface Membrane ◽  
Giant Cells ◽  
Micropipette Aspiration ◽  
Electron Microscopic ◽  
Normal Cells ◽  
Glass Slides ◽  
Resistance To Deformation ◽  
Microscopic Studies ◽  
Cell Sizing

Previous reports have suggested that platelets from patients with Bernard-Soulier's syndrome (BSS) are not giant cells. Rather, they are normal-sized in suspension, but spread out on glass slides more readily than control cells, yielding the impression of being giant. The present study has used cell sizing techniques, electron microscopy, and micropipette aspiration to evaluate platelets from three patients with BSS. Cell sizing techniques revealed that BSS platelets were considerably larger than normal. The increased size was confirmed in electron microscopic studies of BSS platelets fixed in suspension. However, the BSS platelets did not contain increased amounts of internalized surface membrane considered to be the source of membrane necessary for excessive spreading. A possible explanation for increased spreading of BSS platelets was found in studies of their resistance to deformation in micropipettes. BSS platelets were much less resistant to deformation than normal cells or other abnormal platelets when aspirated under the same negative pressure. Their unusual deformability may explain the tendency of BSS platelets to spread more readily than normal cells on glass slides.

scholarly journals The Natural History of Uterine Leiomyomas: Light and Electron Microscopic Studies of Fibroid Phases, Interstitial Ischemia, Inanosis, and Reclamation

2013 ◽  
Vol 2013 ◽  
pp. 1-20 ◽  
Author(s):  
Gordon P. Flake ◽  
Alicia B. Moore ◽  
Deloris Sutton ◽  
Grace E. Kissling ◽  
John Horton ◽  
...  
Keyword(s):  
Cell Death ◽  
Uterine Leiomyomas ◽  
Electron Microscopic ◽  
Nutritional Deprivation ◽  
Limited Life ◽  
Microscopic Studies ◽  
History Of ◽  
End Stage ◽  
Dying Cells ◽  
Phagocytic Reaction

We propose, and offer evidence to support, the concept that many uterine leiomyomas pursue a self-limited life cycle. This cycle can be arbitrarily divided on the basis of morphologic assessment of the collagen content into 4 phases: (1) proliferation, (2) proliferation and synthesis of collagen, (3) proliferation, synthesis of collagen, and early senescence, and (4) involution. Involution occurs as a result of both vascular and interstitial ischemia. Interstitial ischemia is the consequence of the excessive elaboration of collagen, resulting in reduced microvascular density, increased distance between myocytes and capillaries, nutritional deprivation, and myocyte atrophy. The end stage of this process is an involuted tumor with a predominance of collagen, little to no proliferative activity, myocyte atrophy, and myocyte cell death. Since many of the dying cells exhibit light microscopic and ultrastructural features that appear distinct from either necrosis or apoptosis, we refer to this process as inanosis, because it appears that nutritional deprivation, or inanition, is the underlying cause of cell death. The disposal of myocytes dying by inanosis also differs in that there is no phagocytic reaction, but rather an apparent dissolution of the cell, which might be viewed as a process of reclamation as the molecular contents are reclaimed and recycled.

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