Paracrystalline structures in the oocytes of a mouse, mus cervicolor poppaeus

Large and multiple paracrystalline bodies exist in the cytoplasm of the oocytes of a wild mouse strain originating in Thailand, Mus cervicolor poppaeus. The structural details of these crystals are identical to much smaller paracrystalline structures described in preimplantation embryos of the laboratory mouse, Mus musculus (Enders and Schlafke, 1965; Calarco and Brown, 1969; Calarco and Szöllösi, 1973). These latter often occur in close proximity or even in contact with the rough endoplasmic reticulum (RER). A number of A-type virus like particles bud into these cirternae. In spite of the fact that thus far no intracisternal A-type particles were recognized in oocytes of Mus cervicolor poppaeus the similarity between the two paracrystalline structures warrants their description and comparison.In the cytoplasm of oocytes we collected by puncture from large Graafian follicles of Mus cervicolor poppaeus. In one micron sections stained with Richardson´s strain (Richardson et al., 1960), rhomboidal crystalline bodies of various sizes can be recognized. The crystalloids stain intensely blue (Fig. 1).

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The Morphology of Normal and Abberrant Murine Type Leukemia Virus and Its Relationship to Ribosomes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094632 ◽

1972 ◽

Vol 30 ◽

pp. 274-275

Author(s):

T. N. Tahmisian ◽

E. J. Ainsworth

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Rough Endoplasmic Reticulum ◽

Intraperitoneal Injection ◽

Leukemia Virus ◽

X Rays ◽

Type Virus ◽

Unit Membrane ◽

Viral Membrane ◽

Viral Development

Studies are in progress to characterize a transmissible leukemia and the causal agent which is presumably viral. The neoplasm, originally isolated from an aged irradiated B6CF1, mouse, has been transmitted in irradiated (200 R; 250 kVp X rays) weanling mice by intraperitoneal injection of millipore filtered (0.45μ) supernatant from spleen cell suspensions. At 22 days after filtrate injection in syngeneic mice, spleen, lymph nodes, and thymus were removed and prepared for electron microscopy to determine the presence of virus and morphology of viral development.The ultrastructure of cells from the above organs showed viral infection by a murine type leukemia virus with many “C” type buds 90 to 110nm formed in the cisternae of the rough endoplasmic reticulum (Fig. 1). The limiting membrane of the “C” type virus, apparently isolating it from the cytoplasm, is the unit membrane from the rough endoplasmic reticulum. The viral membrane is not invariably intact.

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Homology of p53 intronic sequences between four laboratory mouse strains and Japanese wild mouse (Mus musculus molossinus Mishima)

Genome ◽

10.1139/g94-145 ◽

1994 ◽

Vol 37 (6) ◽

pp. 1022-1026 ◽

Cited By ~ 1

Author(s):

Masayuki Tokumitsu ◽

Katsuhiro Ogawa

Keyword(s):

Loss Of Heterozygosity ◽

Mus Musculus ◽

Laboratory Mouse ◽

Wild Mouse ◽

P53 Gene ◽

Inbred Strains ◽

Mouse Strains ◽

Restriction Enzyme Digestion ◽

Laboratory Mice ◽

Wild Mice

Strain variation in the mouse p53 gene sequences was investigated in various regions of the gene in 14 inbred strains of laboratory mice and one Japanese wild mouse strain (Mus musculus molossinus Mishima, M. MOL-MSM). Nucleotides within p53 introns 1 and 7, found to be identical in 10 of the laboratory strains (129/J, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6J, CBA/J, CE/J, NZB, and SWR/J), were substituted for other nucleotide sequences in common with M. MOL-MSM and the four other strains (DBA/1J, DBA/2J, I/LnJ, and P/J). The latter were documented to have originated from a common ancestor. These observations thus suggested the possibility that the p53 gene may have become substituted by outcrossing of this ancestral strain with Asian mice; this is presumably related to the documentation that Japanese mice brought to western countries were used as laboratory mice early in this century. To establish p53 gene heterozygosity, female C3H/HeJ and male DBA/2J mice were mated to produce F1, hybrids (C3D2F1,). Electrophoresis of PCR fragments including polymorphic regions with or without restriction enzyme digestion, allowed clear distinction of paternal and maternal p53 alleles. These markers, therefore, should be useful for studying the loss of heterozygosity of the p53 gene during the carcinogenic process.Key words: p53 gene, polymorphism, Japanese wild mice, laboratory mice, loss of heterozygosity.

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Modification of Pineal Ultrastructure by Exogenous Hormones

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060866 ◽

1967 ◽

Vol 25 ◽

pp. 170-171

Author(s):

R. A. Turner ◽

A. E. Rodin ◽

D. K. Roberts

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Rough Endoplasmic Reticulum ◽

Estradiol Benzoate ◽

Female Rats ◽

List Type ◽

Type Number ◽

Pregnant Rats ◽

Gonadal Atrophy ◽

Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

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Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143468 ◽

1986 ◽

Vol 44 ◽

pp. 368-369

Author(s):

V.J. Montpetit ◽

S. Dancea ◽

L. Tryphonas ◽

D.F. Clapin

Keyword(s):

Animal Model ◽

Endoplasmic Reticulum ◽

Dorsal Root Ganglia ◽

Rough Endoplasmic Reticulum ◽

Dorsal Root ◽

Vitamin B6 ◽

Morphological Changes ◽

Model System ◽

Sensory Nerves ◽

Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

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