Ultrastructural comparison of cynomolgus monkey liver samples following immersion fixation in different primary fixatives

1987 ◽  
Vol 45 ◽  
pp. 712-713
Author(s):  
M. A. Egy
Keyword(s):  
Electron Microscopy ◽  
Young Adult ◽  
Normal Control ◽  
Adult Male ◽  
Cynomolgus Monkey ◽  
Hepatic Tissue ◽  
Pathology Laboratory ◽  
Cynomolgus Monkeys ◽  
Monkey Liver ◽  
Electron Microscopist

This study was undertaken to find an optimal primary fixative for the assessment of ultrastructural morphology of hepatic tissue from cynomolgus monkeys under common constraints in a pathology laboratory. No single fixative for electron microscopy is suitable for every organ, species or application. Immersion fixation is prescribed when a limited amount of tissue is available from biopsies or when tissues must be shared with other investigators. In addition, tissues are often collected at another location and then sent to the electron microscopist so a delay is incurred before processing is completed. The ultrastructural effects of eight fixatives and two designed delays were evaluated in this study.Fresh hepatic tissue was excised from the left lateral hepatic lobe of normal control young adult male cynomolgus monkeys and fixed by immersion according to 8 protocols.

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Alteration of Mitochondria in Oxythiamine-Induced, Acute Thiamine Deficiency in the Mouse

1976 ◽  
Vol 34 ◽  
pp. 284-285
Author(s):  
Itaru Watanabe ◽  
Dante G. Scarpelli
Keyword(s):  
Electron Microscopy ◽  
Adult Male ◽  
Thiamine Deficiency ◽  
Light And Electron Microscopy ◽  
Fatty Change ◽  
Deficient Diet ◽  
Subcutaneous Injections ◽  
Swiss Mice ◽  
Time Period ◽  
Ad Libitum

Acute thiamine deficiency was produced in mice by the administration of oxythiamine, a thiamine analogue, superimposed upon a thiamine deficient diet. Adult male Swiss mice (30 gm. B.W.) were fed with a thiamine deficient diet ad libitumand were injected with oxythiamine (170 mg/Kg B.W.) subcutaneously on days 4 and 10. On day 11, severe lassitude and anorexia developed, followed by death within 48 hours. The animals treated daily with subcutaneous injections of thiamine (300 μg/Kg B.W.) from day 11 through 15 were kept alive. Similarly, feeding with a diet containing thiamine (600 μg/Kg B.W./day) from day 9 through 17 reversed the condition. During this time period, no fatal illness occurred in the controls which were pair-fed with a thiamine deficient diet.The oxythiamine-treated mice showed a significant enlargement of the liver, which weighed approximately 1.5 times as much as that of the pair-fed controls. By light and electron microscopy, the hepatocytes were markedly swollen due to severe fatty change and swelling of the mitochondria.

Scanning electron microscopy of the integument of schistosoma rodhaini

1986 ◽  
Vol 44 ◽  
pp. 132-133
Author(s):  
P. Evers ◽  
C. Schutte ◽  
C. D. Dettman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Oral Sucker ◽  
Adult Male ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Sensory Receptors ◽  
East African ◽  
Dorsal Midline ◽  
Scanning Electron

S.rodhaini (Brumpt 1931) is a parasite of East African rodents which may possibly hybridize with the human schistosome S. mansoni. The adult male at maturity measures approximately 3mm long and possesses both oral and ventral suckers and a marked gynaecophoric canal. The oral sucker is surrounded by a ring of sensory receptors with a large number of inwardly-pointing spines set into deep sockets occupying the bulk of the ventral surface of the sucker. Numbers of scattered sensory receptors are found on both dorsal and ventral surfaces of the head (Fig. 1) together with two conspicuous rows of receptors situated symmetrically on each side of the midline. One row extends along the dorsal surface of the head midway between the dorsal midline and the lateral margin.

Infected urachal duct cyst in a young adult male

2021 ◽  
Vol 22 ◽  
pp. 100975
Author(s):  
Ellen Duncan ◽  
Suneet Bhansali ◽  
Ee Tein Tay
Keyword(s):  
Young Adult ◽  
Adult Male ◽  
Duct Cyst ◽  
Young Adult Male

scholarly journals Efficacy of Electromyographic-Biofeedback Supplementation Training with Patellar Taping on Quadriceps Strengthening in Patellofemoral Pain Syndrome among Young Adult Male Athletes

2021 ◽  
Vol 18 (9) ◽  
pp. 4514
Author(s):  
Asma Alonazi ◽  
Shahnaz Hasan ◽  
Shahnawaz Anwer ◽  
Azfar Jamal ◽  
Suhel Parvez ◽  
...  
Keyword(s):  
Young Adult ◽  
Adult Male ◽  
Functional Disability ◽  
Isometric Exercise ◽  
Quadriceps Strength ◽  
Control Group ◽  
Male Athletes ◽  
Young Adult Male ◽  
Patellar Taping ◽  
Experimental Group

This study compares the effects of electromyographic-biofeedback (EMG-BF)-guided isometric quadriceps strengthening with patellar taping and isometric exercise alone in patellofemoral pain syndrome (PFPS) among young adult male athletes. Sixty young adult male athletes with PFPS participated in the study. Participants were randomly divided into two groups: (1) EMG-BF-guided isometric exercise training with patellar taping (experimental group, n = 30), and (2) sham EMG-BF training with an isometric exercise program (control group, n = 30). Participants conducted their respective exercise programs for five days per week across four weeks. Study outcomes were pain (measured by the visual analog scale), functional disability (measured by the Kujala Anterior Knee Pain scale), and quadriceps strength (measured by an ISOMOVE dynamometer). Measurements were taken at baseline, Week 2, Week 4, and during a follow-up at Week 6. The experimental group demonstrated significantly lower VAS score at Weeks 2 and 4 compared to that of the control group (p = 0.008 and 0.0005, respectively). The score remained significantly lower at the Week 6 follow-up compared to the control group (p = 0.0005). There were no differences in knee function at Weeks 2 and 4 between the two groups (p = 0.086 and 0.171, respectively); however, the experimental group showed significantly better knee function at Week 6 compared to the control group (p = 0.002). There were no differences in quadriceps strength at Week 2 between the two groups (p = 0.259); however, the experimental group demonstrated significantly higher quadriceps strength at Weeks 4 and 6 compared to the control group (p = 0.0008). Four weeks of EMG-BF supplementation training with patellar taping demonstrated significant improvements in pain intensity, functional disability, and quadriceps muscle strength in young adult male athletes with PFPS.

Client-Clinician Relationships and Concomitant Factors in Stuttering Therapy

1966 ◽  
Vol 9 (2) ◽  
pp. 194-207 ◽  
Author(s):  
Eugene B. Cooper
Keyword(s):  
Young Adult ◽  
Therapeutic Relationship ◽  
Graduate Student ◽  
Adult Male ◽  
Personality Characteristics ◽  
Young Adult Male ◽  
Concomitant Factors ◽  
Client Progress ◽  
The Relationship ◽  
As If

Interrelationships among client progress in stuttering therapy, the nature of the affect interchange between client and clinician, and certain personality characteristics of both client and clinician were studied. Sixteen young adult male stutterers and their 11 graduate student clinicians served as subjects. Results support observations that the clinicians' and clients' personalities are significant variables in the stuttering therapy situation, support observations that important similarities exist between stuttering therapy and psychotherapy, and suggest that it is more accurate to note stages in the therapeutic relationship, rather than to characterize the relationship as if it were the same throughout therapy.

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