Increase of the content of QP47 (a desiccation-associated nuclear protein) in embryo cells during maturation of pea seeds

1994 ◽  
Vol 4 (4) ◽  
pp. 421-429 ◽  
Author(s):  
Donato Chiatante ◽  
Paola Brusa
Keyword(s):  
Cell Proliferation ◽  
Nuclear Protein ◽  
Early Stage ◽  
Seed Maturation ◽  
Radicle Elongation ◽  
Embryo Cells ◽  
Last Stage ◽  
Pea Seeds ◽  
Metabolic Activities

AbstractA nuclear protein (QP47) is synthesized during the last stage of seed maturation when the embryo cells start to dehydrate and enter a condition of metabolic quiescence. This protein is localized in the nucleoplasm surrounding the chromosomes. The correlation existing between the synthesis of QP47 and arrest of cell proliferation, suggests that the presence of this protein in the nucleus could influence its metabolic activities. This hypothesis is supported by the fact that degradation of this protein precedes resumption of cell proliferation during the early stage of radicle elongation.

PCNA and total nuclear protein content as markers of cell proliferation in pea tissue

1992 ◽  
Vol 102 (1) ◽  
pp. 71-78 ◽  
Author(s):  
SANDRA CITTERIO ◽  
SERGIO SGORBATI ◽  
MARISA LEVI ◽  
BRUNO MARIA COLOMBO ◽  
ELIO SPARVOLI
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Protein Content ◽  
Time Course ◽  
Nuclear Protein ◽  
Flow Cytometric Analysis ◽  
Nuclear Antigen ◽  
Cycle Phase ◽  
Proliferating Cells ◽  
Embryo Cells

The identification of cell proliferation markers has been shown to be a useful tool with which to study basic mechanisms of cell cycle progression. The use of immunofluorescence techniques revealed the presence of the proliferating cell nuclear antigen (PCNA) in pea tissue, where we observed a high PCNA expression in proliferating cells of the root meristem compared to noncycling cells of the differentiated leaf. The presence of PCNA was monitored also during the time-course of seed germination, before, during and after the cell cycle resumption of the embryo cells. PCNA is present in embryo cells not only during and after resumption of the cell cycle but also before, when cells have not yet begun replicating their genome. A bivariate flow cytometric analysis of DNA and nuclear protein content was used to localize precisely the cells of the examined pea tissues in different cell cycle phase subcompartments. A high correlation was found between the degree of cell proliferation and the protein content of G1 nuclei, on the one hand, and the percentage of PCNA positive cells on the other.

scholarly journals Proinflammatory signal suppresses proliferation and shifts macrophage metabolism from Myc-dependent to HIF1α-dependent

2016 ◽  
Vol 113 (6) ◽  
pp. 1564-1569 ◽  
Author(s):  
Lingling Liu ◽  
Yun Lu ◽  
Jennifer Martinez ◽  
Yujing Bi ◽  
Gaojian Lian ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Hypoxia Inducible Factor ◽  
Transcriptional Program ◽  
Biosynthetic Activity ◽  
Environmental Signals ◽  
Inhibition Of Glycolysis ◽  
Metabolic Activities ◽  
And Shifts

As a phenotypically plastic cellular population, macrophages change their physiology in response to environmental signals. Emerging evidence suggests that macrophages are capable of tightly coordinating their metabolic programs to adjust their immunological and bioenergetic functional properties, as needed. Upon mitogenic stimulation, quiescent macrophages enter the cell cycle, increasing their bioenergetic and biosynthetic activity to meet the demands of cell growth. Proinflammatory stimulation, however, suppresses cell proliferation, while maintaining a heightened metabolic activity imposed by the production of bactericidal factors. Here, we report that the mitogenic stimulus, colony-stimulating factor 1 (CSF-1), engages a myelocytomatosis viral oncogen (Myc)-dependent transcriptional program that is responsible for cell cycle entry and the up-regulation of glucose and glutamine catabolism in bone marrow-derived macrophages (BMDMs). However, the proinflammatory stimulus, lipopolysaccharide (LPS), suppresses Myc expression and cell proliferation and engages a hypoxia-inducible factor alpha (HIF1α)-dependent transcriptional program that is responsible for heightened glycolysis. The acute deletion of Myc or HIF1α selectively impaired the CSF-1– or LPS-driven metabolic activities in BMDM, respectively. Finally, inhibition of glycolysis by 2-deoxyglucose (2-DG) or genetic deletion of HIF1α suppressed LPS-induced inflammation in vivo. Our studies indicate that a switch from a Myc-dependent to a HIF1α-dependent transcriptional program may regulate the robust bioenergetic support for an inflammatory response, while sparing Myc-dependent proliferation.

Inhibitory Effects of Purple Sweet Potato Leaf Extract on the Proliferation and Lipogenesis of the 3T3-L1 Preadipocytes

2015 ◽  
Vol 43 (05) ◽  
pp. 915-925 ◽  
Author(s):  
Shou-Lun Lee ◽  
Hsien-Kuang Lee ◽  
Ting-Yu Chin ◽  
Ssu-Chieh Tu ◽  
Ming-Hsun Kuo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Sweet Potato ◽  
Early Stage ◽  
Water Extract ◽  
Potato Leaf ◽  
Purple Sweet Potato ◽  
Pre Treatment ◽  
Dose Dependent Decrease ◽  
Dose Dependent

Purple sweet potato leaves (PSPLs) are healthy vegetable that is rich in anti-oxidants. A solution of boiling water extract of PSPL (PSPLE) is believed to be able to prevent obesity and metabolic syndrome in the countryside of Taiwan, but its efficacy has not yet been verified. The purpose of this study was to investigate the possible anti-adipogenesis effect of PSPLE in vitro. PSPLE was used to treat the 3T3-L1 cells, and the effects on cell proliferation and adipogenesis were investigated. The results showed that PSPLE caused a dose-dependent decrease in the cell proliferation of 3T3-L1 preadipocytes, but did not alter the cell viability. In addition, PSPLE induced ERK inactivation in the 3T3-L1 preadipocytes. Furthermore, pre-treatment of confluent 3T3-L1 cells with PSPLE led to reduced lipid accumulation in differentiated 3T3-L1 cells. The inhibition of lipogenesis could result from the PSPLE-induced down-regulation of the expression of the C/EBPα and SREBP-1 transcription factors during 3T3-L1 adipocyte differentiation. These results suggest that PSPLE not only inhibits cell proliferation at an early stage but also inhibits adipogenesis at a later stage of the differentiation program.

scholarly journals HCaRG, a Novel Calcium-regulated Gene Coding for a Nuclear Protein, Is Potentially Involved in the Regulation of Cell Proliferation

2000 ◽  
Vol 275 (41) ◽  
pp. 32234-32243 ◽  
Author(s):  
Nicolas Solban ◽  
Hong-Peng Jia ◽  
Sylvie Richard ◽  
Sandra Tremblay ◽  
Alison M. Devlin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nuclear Protein ◽  
Gene Coding

scholarly journals Analysis of Activation Process of Carbon Black Based on Structural Parameters Obtained by XRD Analysis

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 153
Author(s):  
Sang-Min Lee ◽  
Sang-Hye Lee ◽  
Jae-Seung Roh
Keyword(s):  
Carbon Black ◽  
Early Stage ◽  
Structural Parameters ◽  
Xrd Analysis ◽  
Activation Process ◽  
Co2 Gas ◽  
Carbon Black Surface ◽  
Black Surface ◽  
Last Stage ◽  
Pore Properties

In the present study, carbon black activated by CO2 gas was examined through XRD analysis, especially with regard to changes in its structural parameters. Based on the results, its activation process was thoroughly analyzed. The activation process was controlled by isothermally activating the carbon black inside a reaction tube through which CO2 gas flowed. With this approach, the degree of activation was varied as desired. At an early stage of the activation process, the amorphous fraction on the carbon black surface was preferentially activated, and later the less-developed crystalline carbon (LDCC) region inside the carbon black particles started to be activated. The latter process was attributable to the formation of pores inside the carbon black particles. As the activation process proceeded further, the more-developed crystalline carbon (MDCC) region started to be activated, thereby causing the pores inside the carbon black particles to grow larger. At the last stage of the activation process, La was found to be decreased to about 40 Å. This implied that the edges of the graphite crystals had been activated, thus causing the internal pores to grow and coalesce into larger pores. Activated conductive Super-P with enhanced pore properties is expected to have wide applications.

scholarly journals Translocation of protein tyrosine phosphatase Pez/PTPD2/PTP36 to the nucleus is associated with induction of cell proliferation

2000 ◽  
Vol 113 (17) ◽  
pp. 3117-3123 ◽  
Author(s):  
C. Wadham ◽  
J.R. Gamble ◽  
M.A. Vadas ◽  
Y. Khew-Goodall
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Nuclear Protein ◽  
Vascular Endothelial Cells ◽  
Tyrosine Phosphatase ◽  
Subcellular Localisation ◽  
Vascular Endothelial ◽  
Proliferating Cells ◽  
Wound Edge

Pez is a non-transmembrane tyrosine phosphatase with homology to the FERM (4.1, ezrin, radixin, moesin) family of proteins. The subcellular localisation of Pez in endothelial cells was found to be regulated by cell density and serum concentration. In confluent monolayers Pez was cytoplasmic, but in cells cultured at low density Pez was nuclear, suggesting that it is a nuclear protein in proliferating cells. This notion is supported by the loss of nuclear Pez when cells are serum-starved to induce quiescence, and the rapid return of Pez to the nucleus upon refeeding with serum to induce proliferation. Vascular endothelial cells normally exist as a quiescent confluent monolayer but become proliferative during angiogenesis or upon vascular injury. Using a ‘wound’ assay to mimic these events in vitro, Pez was found to be nuclear in the cells that had migrated and were proliferative at the ‘wound’ edge. TGFbeta, which inhibits cell proliferation but not migration, inhibited the translocation of Pez to the nucleus in the cells at the ‘wound’ edge, further strengthening the argument that Pez plays a role in the nucleus during cell proliferation. Together, the data presented indicate that Pez is a nuclear tyrosine phosphatase that may play a role in cell proliferation.

scholarly journals E2FA and E2FB transcription factors coordinate cell proliferation with seed maturation

Development ◽  
2019 ◽  
Vol 146 (22) ◽  
pp. dev179333 ◽  
Author(s):  
Tünde Leviczky ◽  
Eszter Molnár ◽  
Csaba Papdi ◽  
Erika Őszi ◽  
Gábor V. Horváth ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factors ◽  
Seed Maturation
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