Examination of Structural Differences in Gill Vasculature Among Some Batiod Elasmobranchs Using Corrosion Casting and SEM

2001 ◽  
Vol 7 (S2) ◽  
pp. 1208-1209
Author(s):  
R.L. Sherman
Keyword(s):  
Corpus Cavernosum ◽  
Circulatory System ◽  
Gross Anatomy ◽  
Corrosion Casting ◽  
Mixed Species ◽  
Raja Erinacea ◽  
Scyliorhinus Canicula ◽  
Structural Differences ◽  
Vascular Arcade ◽  
Gill Structure

Classic studies of gill vasculature indicate the gross anatomy of elasmobranch gills is similar in most species, and in general, resembles those of teleosts. However, studies of fine gill structure examining arterio-arterial pathways in several species of batoid elasmobranchs (Order: Rajiiformes, Suborder: Myliobatoidei) have reported some structural differences. These differences include the presence, in urolophids (Urolophus jamaicensis, U. mucosus, U. paucimaculatus), of 1) a tip channel, possibly an extension of the afferent filament artery that runs along the top of the filament corpus cavernosum, and 2) a vascular arcade, a vessel which connects the afferent filament arteries of a hemibranch near the tip of each filament (FIG. I). Until recently these structures were believed to be unique to the urolophids as they were not previously found in either rajids (Raja erinacea, R. clavata) or the shark Scyliorhinus canicula. Animals of mixed species and sexes were collected, perfused, and acrylic casts made of the circulatory system.

scholarly journals An accessory organ of the circulatory system in Sepia and Loligo

1962 ◽  
Vol 42 (2) ◽  
pp. 405-418 ◽  
Author(s):  
J. S. Alexandrowicz
Keyword(s):  
Ganglion Cells ◽  
Circulatory System ◽  
Retractor Muscle ◽  
Muscle Fibres ◽  
Sepia Officinalis ◽  
Motor Innervation ◽  
Vasomotor System ◽  
Inner Surface ◽  
Nerve Bundles ◽  
Structural Differences

SUMMARYAn accessory organ of the circulatory system has been found in Sepia officinalis and Loligo forbesi, situated on the inner surface of the mantle around the posterior pallial vessels. It is spheroidal in shape and consists chiefly of muscle fibres forming bundles anastomosing with one another, most of them taking a general circular course round the artery and the vein. In Sepia the muscle bundles are more loosely arranged, being imbedded in connective tissue of soft consistency; they are not continuous either with the musculature of the vessels or with that of the mantle, and show certain structural differences from them. The nerves approaching this organ from various directions carry elements of two systems: (a) fibres given off by the branches innervating the neighbouring mantle muscles; (b) elements of the vasomotor system; these are 3 trunks consisting of fibres of small calibre and containing groups or rows of ganglion cells. One of these trunks associates with one of the main mantle nerves, another runs through the retractor muscle of the branchia. Within the accessory organ they intermingle with the motor fibres, but the nerve bundles passing to the posterior pallial vessels consist of fibres of the vasomotor system only; it is possible that the muscles of the accessory organ are supplied, in addition to their motor innervation, with fibres of the vasomotor system.

Eicosapentaenoic acid in the form of phospholipids exerts superior anti-atherosclerosis effects to its triglyceride form in ApoE−/−mice

2019 ◽  
Vol 10 (7) ◽  
pp. 4177-4188 ◽  
Author(s):  
Ling-Yu Zhang ◽  
Lin Ding ◽  
Hao-Hao Shi ◽  
Jie Xu ◽  
Chang-Hu Xue ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Eicosapentaenoic Acid ◽  
Circulatory System ◽  
Artery Wall ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid ◽  
Structural Differences

EPA-PL was superior to EPA-TG in reducing lesion progression by modulating hepatic lipid metabolism and decreasing inflammation in the artery wall and circulatory system, which might be attributed to the structural differences at the sn-3 position.

The Decapod Crustacean Circulatory System: A Case That Is neither Open nor Closed

2005 ◽  
Vol 11 (1) ◽  
pp. 18-36 ◽  
Author(s):  
Iain J. McGaw
Keyword(s):  
Blue Crab ◽  
Small Diameter ◽  
Circulatory System ◽  
Decapod Crustacean ◽  
Ventral Surface ◽  
The Body ◽  
Corrosion Casting ◽  
Scanning Electron Micrographs ◽  
System A ◽  
Blind Ending

Historically, the decapod crustacean circulatory system has been classed as open. However, recent work on the blue crab,Callinectes sapidus, suggests the circulatory system may be more complex than previously described. Corrosion casting techniques were refined and used to map the circulatory system of a variety of crab species (order: Decapoda; family: Cancridae) to determine if the complexity observed in the blue crab was present in other species. Seven arteries arose from the single chambered heart. The anterior aorta, the paired anterolateral arteries, and the paired hepatic arteries exited from the anterior aspect of the heart. The small-diameter posterior aorta exited posteriorly from the heart. Exiting from the ventral surface of the heart, the sternal artery branched to supply the legs and mouthparts of the crab. These arteries were more complex than previously described, with arterioles perfusing all areas of the body. The arterioles split into fine capillary-like vessels. Most of these capillaries were blind ending. However, in several areas (antennal gland, supraesophageal ganglion) complete capillary beds were present. After passing through the capillary-like vessels, blood drained into a series of sinuses. However, rather than being arbitrary spaces as previously described, scanning electron micrographs showed the sinuses to be distinct units. Most of the sinuses formed a series of flattened membrane-bound lacunae. This complexity may qualify the decapod crustacean circulatory system as one that is “partially closed” rather than open.

scholarly journals Gross anatomy and ultrasonography of the udder in goat

2017 ◽  
Vol 34 (03) ◽  
pp. 137-142
Author(s):  
Z. Adam ◽  
G. Ragab ◽  
A. Awaad ◽  
M. Tawfiek ◽  
M. Maksoud
Keyword(s):  
Pelvic Floor ◽  
Abdominal Wall ◽  
Gross Anatomy ◽  
Corrosion Casting ◽  
Lactation Period ◽  
Anatomical Dissection ◽  
Pathological Conditions ◽  
Precise Knowledge ◽  
Dairy Animals ◽  
Teat Canal

Abstract Introduction: The udder is a very important structural and physiological component in all dairy animals, so the precise knowledge of its normal gross morphology is fundamental for the clinical examination. Objective: The current study aimed to clarify the gross anatomical characteristics and ultrasonographic findings of the udder in Egyptian native breeds of goat (Baladi goat). Materials and Methods: Thirteen healthy Baladi goats during lactation period were grossly investigated and then they were examined through B-mode ultrasonography. Two specimens were used for corrosion casting and the remaining specimens were subjected to the anatomical dissection. Results: The gross anatomical investigation revealed that the udder of goat was consisted of two halves; each one had mammary body and teat, and it was suspended in the ventral abdominal wall and pelvic floor through the medial and lateral suspensory laminae. Moreover, each half was composed of a single mammary unit which included the mammary glandular parenchyma, lactiferous ducts, lactiferous sinus and teat canal ended by a teat orifice. These mammary structures showed variant echogenicity during ultrasonographic examination according to their reflective intensity to the ultrasound. Conclusion: The morphological features and ultrasonographic findings obtained in this study could assist in the further diagnosis of several pathological conditions of the udder of goat.

scholarly journals Ameliorative effects of curcumin on microvascular complicationin streptozotocin-induced diabetic rat penis

2019 ◽  
Vol 9 (2) ◽  
pp. 92
Author(s):  
Wipapan Khimmaktong ◽  
Manaras Komolkriengkrai ◽  
Piyakorn Boonyoung ◽  
Vipavee Anupunpisit
Keyword(s):  
Morphological Changes ◽  
Histological Study ◽  
Corpus Cavernosum ◽  
Vascular Diseases ◽  
Diabetic Rats ◽  
Collagen Fibers ◽  
Venous Sinus ◽  
Diabetic Rat ◽  
Corrosion Casting ◽  
Venous Sinuses

Background: Vascular diseases from diabetes typically result in conditions that interfere with the microvascular functions, such as erectile dysfunction (ED) of the penis. It is unrevealed which morphological changes the microvasculature of the penis may result from the treatment and supplementation of curcumin.Objective: This study aims to determine the effects of curcumin, the active compound of turmeric, on penile microvasculature in experimentally induced diabetic rats.Methods:  Rat were divided into three groups, control © and diabetic (DM). Diabetic rats supplemented with curcumin (DMC) (200 mg/kg BW). For induction of a diabetic condition, rats were injected with a single intraperitoneal injection of streptozotocin (60 mg/kg BW). A histological study and vascular corrosion casting along with scanning electron microscopy (SEM) were used to determine a morphological change of microvessels in the penis of rats after 8 weeks of experiments.Results: The histological investigations of penile tissue of DM group revealed thickly lined venous sinuses with smooth but sparse muscle layers. Dorsal arteries (DA) and deep dorsal veins (DV) of the penis were present of narrow lumen and showed thickening of collagen fibers at their wall of tunica media. An increasing thickness of collagen fibers was observed. Vascular corrosion casting revealed critically decreased diameters of DM venous sinus in the penis’ glans, which were observed as shrunken and partially lacking in the distal area of the glans. The diameter of venous sinuses in corpus cavernosum (CC) of DM rats were dilated and the density of venous sinuses was decreased. Interestingly, the signs of vessel restoration and improvement were presented by increasing diameters and recovering with healthy and nourished DA, DV, venous sinus at the area of the glans, and CC of the penis in DMC groups. Conclusion: The results have shown that the effects of curcumin could improve the destruction of the penile microvasculature in induced diabetic rats by STZ. Keywords: Diabetes mellitus, streptozotocin, curcumin, penis, Microvasculature, vascular corrosion cast

High Resolution Observations of Ion-Milling Induced Transformation in Synthetic Hollandses

1981 ◽  
Vol 39 ◽  
pp. 114-115 ◽  
Author(s):  
T. J. Headley
Keyword(s):  
High Resolution ◽  
Radioactive Waste Disposal ◽  
Ion Milling ◽  
Minor Elements ◽  
Waste Forms ◽  
Carbon Substrates ◽  
Structural Differences ◽  
Oxide Phases ◽  
Argon Ions ◽  
High Resolution Microscopy

Oxide phases having the hollandite structure have been identified in multiphase ceramic waste forms being developed for radioactive waste disposal. High resolution studies of phases in the waste forms described in Ref. [2] were initiated to examine them for fine scale structural differences compared to natural mineral analogs. Two hollandites were studied: a (Ba,Cs,K)-titan-ate with minor elements in solution that is produced in the waste forms, and a synthesized BaAl2Ti6O16 phase containing ∼ 4.7 wt% Cs2O. Both materials were consolidated by hot pressing at temperatures above 1100°C. Samples for high resolution microscopy were prepared both by ion-milling (7kV argon ions) and by crushing and dispersing the fragments on holey carbon substrates. The high resolution studies were performed in a JEM 200CX/SEG operating at 200kV.

Some quantitative applications of vascular corrosion casting

1992 ◽  
Vol 50 (1) ◽  
pp. 738-739
Author(s):  
Fred E. Hossler
Keyword(s):  
Blood Vessels ◽  
Nuclear Orientation ◽  
Three Dimensional ◽  
Surrounding Tissue ◽  
Confocal Laser ◽  
Corrosion Casting ◽  
Venous Valve ◽  
Casting Technique ◽  
Low Viscosity ◽  
Quantitative Measurements

Preparation of replicas of the complex arrangement of blood vessels in various organs and tissues has been accomplished by infusing low viscosity resins into the vasculature. Subsequent removal of the surrounding tissue by maceration leaves a model of the intricate three-dimensional anatomy of the blood vessels of the tissue not obtainable by any other procedure. When applied with care, the vascular corrosion casting technique can reveal fine details of the microvasculature including endothelial nuclear orientation and distribution (Fig. 1), locations of arteriolar sphincters (Fig. 2), venous valve anatomy (Fig. 3), and vessel size, density, and branching patterns. Because casts faithfully replicate tissue vasculature, they can be used for quantitative measurements of that vasculature. The purpose of this report is to summarize and highlight some quantitative applications of vascular corrosion casting. In each example, casts were prepared by infusing Mercox, a methyl-methacrylate resin, and macerating the tissue with 20% KOH. Casts were either mounted for conventional scanning electron microscopy, or sliced for viewing with a confocal laser microscope.

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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