Fish Erythrocytes as Biomarkers for the Toxicity of Sublethal Doses of an Azo Dye, Basic Violet-1 (CI: 42535)

2014 ◽  
Vol 21 (1) ◽  
pp. 264-273 ◽  
Author(s):  
Kirandeep Kaur ◽  
Arvinder Kaur
Keyword(s):  
Azo Dye ◽  
Labeo Rohita ◽  
Abnormal Behavior ◽  
Important Food ◽  
Short Term ◽  
Sem Study ◽  
Test Fish ◽  
Food Fish ◽  
Sublethal Doses ◽  
Scanning Electron

AbstractThe aim of the present study was to investigate poikilocytosis in Labeo rohita (an important food fish) as an early indicator of stress due to an azo dye, Basic Violet-1 (CI: 42535). This dye was observed to be very toxic to test fish (96 h LC50 as0.45 mg/L dye). Fish were given short-term (96 h) and subchronic (150 days) exposures to the dye, and poikilocytosis was recorded under light and scanning electron microscopy (SEM). Light microscopy helped in identification of micronuclei along with irregularities, notches, blebs, lobes, crenation, clumps, chains, spherocytes, vacuolation, and necrosis in erythrocytes. However, SEM indicated shrinkage, oozing of cytoplasm, and several new abnormal shapes including marginal foldings, discocytes, keratocytes, dacrocytes, degmacytes, acanthocytes, echinocytes, protuberances, stomatocytes, drepanocytes, holes in the membrane, stippling/spicules, crescent-shaped cells, triangular cells, and pentagonal cells. Earlier studies speculated changes in the membrane to be responsible for clumping and chaining of erythrocytes, whereas the present SEM study clearly indicates that oozing out of cytoplasm is also responsible for the formation of chains and clumps. This study also shows that erythrocytes exhibit pathological symptoms before the appearance of other external symptoms such as abnormal behavior or mortality of fish. There was a dose- and duration-dependent increase; therefore, poikilocytosis, especially echinocytes, spherocytes, and clumps, can act as a biomarker for the stress caused by azo dyes.

scholarly journals Length-weight relationships of the Indian Major Carp, Labeo rohita (Cypriniformes: Cyprinidae) from six drainages of Ganga basin, India

2015 ◽  
Vol 7 (1) ◽  
pp. 71-77
Author(s):  
Javaid Iqbal Mir ◽  
Uttam Kumar Sarkar ◽  
Om Prakash Gusain ◽  
Arvind Kumar Dwivedi
Keyword(s):  
Labeo Rohita ◽  
Main Channel ◽  
Coefficient Of Determination ◽  
Important Food ◽  
Indian Major Carp ◽  
Ganga Basin ◽  
Natural Range ◽  
Food Fish ◽  
Management And Conservation ◽  
Tropical Freshwater

The Indian Major Carp, Labeo rohita, is a geographically widespread and economically important food fish species in tropical freshwater of India and adjacent countries. We studied the lengthweightrelationships of 1 033 specimens collected from the main channel of Ganga river and its five major drainages from March 2009 to July 2012. The length of males ranged from 16 to 92cm (females: 16to 94cm). The growth is allometric positive (b>3) for males, females and pooled sexes. The coefficient of determination (r2) in males ranged from 0,978 to 0,989 and for females from 0,958 to 0,985. Data from field populations are scarce and our results will be useful in the management and conservation of L. rohita populations in its natural range.

Study on Biometric Parameters and Genetic Diversity of Labeo rohita from Harike Wetland-A Ramsar Site

2020 ◽  
Author(s):  
Surjya Narayan Datta ◽  
Armaandeep Kaur ◽  
Anuj Tyagi
Keyword(s):  
Genetic Diversity ◽  
Labeo Rohita ◽  
Coi Gene ◽  
Series Data ◽  
Ramsar Site ◽  
Important Food ◽  
Commercially Important ◽  
Food Fish ◽  
Harike Wetland ◽  
Catch Composition

Background: Harike wetland (31°08¢ N to 31°23¢ N latitudes and 74°90¢ E to 75°12¢ E longitudes) is an internationally important Ramsar site, supports rare, vulnerable and endangered plants, fish and other faunal species. Weed infestation, pollution and encroachment are considered as important threats of its biota thus time series data with respect to fish diversity, catch composition and genetic variability has an utter importance to interpret changes over time. Cyprinidae family of freshwater fishes includes carps is the largest fish family found in Harike wetland comprising around 50% of total fish composition by weight basis and among carps, Rohu (Labeo rohita) is one of the commercially important food fish species available throughout the year and preferred by consumers. As river Beas and Sutlej, two major rivers of Indus river system confluence at Harike thus possibility of variation within fish stock is very high. With this background present study was carried out to evaluate the fish biodiversity and catch composition of Harike wetland. Study also emphasized on biometrics, length –weight relationship and genetic diversity based on mitochondrial marker genes of commercially important food fish Labeo rohita. Methods: Assessment of fish catch composition and diversity were conducted in landing centre adjacent to Harike wetland through Rapid Fisheries Assessment by Market Survey (RFAMS) technique. Fin tissue samples were collected for genetic diversity analysis of L. rohita by cytochrome oxidase subunit I (COI) gene sequencing, From the fish genomic DNA a partial fragment of approximately 655bp was PCR amplified by FishF1 (5'-TCAACCAACCACAAAGACATTGGCAC-3') and FishR1 (5'-TCGACTAATCATAAAGATATCGGC AC-3') primer pair. Calculation of intraspecific mean and pairwise distances was performed by MEGA 6.0 software using the K2P parameters Results: Total 30 species of fishes were recorded from Harike wetland and these belong to 14 families and 21 genera. In L. rohita average weight (Wt), total length (TL), standard length (SL) and forked length (FL) were recorded 2600±5.64g (1700-3600 g), 58.2±5.65 cm (51.3-67.6cm), 48.0±8.54 cm (42.5-55.5cm) and 46.0±0.25 cm (38.0-54.5cm), respectively. Biometric study revealed that sufficient numbers of mature L. rohita are available in wetland. L. rohita established negative algometric growth (b= 2.701); thus species became slender as it increased in length. The pairwise distances ranged from 0.00 to 18.49% with a mean ±S.E value of 4.70%±0.40. Total of 9 haplotypes were observed in L. rohita COI sequences. The diversity in haplotype and nucletide values were observed 0.848 and 0.024, respectively. The present study states that L. rohita stocks in Harike wetland are genetically diverse.

Morphometric SEM study of the effects of ACTH on endothelial fenestrae in the rat adrenal cortex

1984 ◽  
Vol 42 ◽  
pp. 234-235
Author(s):  
J. Curtis ◽  
K. S. Schwartz ◽  
R. P. Apkarian
Keyword(s):  
Anterior Pituitary ◽  
Lipid Droplets ◽  
Adrenocorticotropic Hormone ◽  
Unit Area ◽  
High Density Lipoproteins ◽  
Capillary Endothelium ◽  
Zona Fasciculata ◽  
Sem Study ◽  
Saline Vehicle ◽  
Scanning Electron

A scanning electron microscope (SEM) study was made of the effect of adrenocorticotropic hormone (ACTH) on the size and numbers of fenestrae/unit area in the capillary endothelium of the zona fasciculata (ZF) of the rat adrenal. The stimulatory effect of ACTH on cholesterol uptake via high density lipoproteins in the rat and evidence for the secretion of glucocorticoids by exocytosis of lipid droplets described by Rhodin suggest that endothelial change may accompany these transport phenomena.Twelve rats received two Dexamethasone (DEX) ip injections (25 μg DEX/100 g body wt.), the first at 8 PM and the second at 8 AM the next day, to inhibit the release of endogenous ACTH by the anterior pituitary. The animals were then divided into two groups. Six animals received only saline vehicle and six rats received ACTH (100 ng/100 g body wt.).

scholarly journals Photocatalytical Degradation of Congo Red Azo Dye Using Phosphono-Aminoacid-Cd(II) Pendant Groups Grafted on a Polymeric Support

2019 ◽  
Vol 70 (10) ◽  
pp. 3473-3476 ◽  
Author(s):  
Laura Cocheci ◽  
Lavinia Lupa ◽  
Aniela Pop ◽  
Aurelia Visa ◽  
Bianca Maranescu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Congo Red ◽  
Azo Dye ◽  
Organic Dyes ◽  
Degradation Process ◽  
Polymer Support ◽  
Polymeric Support ◽  
Vis Spectroscopy ◽  
Red Dye ◽  
Scanning Electron

In the present study, a new compound was used for decontaminating water polluted by Congo Red dye. Phosphono-aminoacid-Cd(II) pendant groups grafted on a polymeric support was prepared using impregnation of a polymer support functionalized with phosphono-aminoacid groups with an aqueous solution of Cd(NO3)2x4H2O. The catalyst was characterized by Fourier transform infrared ray, scanning electron microscope, termogravimetric analysis UV-Vis spectroscopy. The results suggest that the studied material could efficiently be used as photocatalyst in the degradation process of waters containing organic dyes under UV-Vis light irradiation.

Craniofacial abnormalities induced by retinoic acid: a preliminary histological and scanning electron microscopic (SEM) study

2000 ◽  
Vol 52 (5) ◽  
pp. 445-453 ◽  
Author(s):  
E.-N. Emmanouil-Nikoloussp ◽  
M. Goret-Nicaise ◽  
Ch. Foroglou ◽  
E. Katsarma ◽  
A. Dhem ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Craniofacial Abnormalities ◽  
Electron Microscopic ◽  
Sem Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

A SEM study of cell shedding during the formation of the area pellucida in the chick embryo

Development ◽  
1981 ◽  
Vol 64 (1) ◽  
pp. 11-22
Author(s):  
Barry Fabian ◽  
Hefzibah Eyal-Giladi
Keyword(s):  
Electron Microscopy ◽  
Central Area ◽  
Ventral Side ◽  
Round Cell ◽  
Physical Force ◽  
Sem Study ◽  
Cell Shedding ◽  
Cytoplasmic Processes ◽  
Scanning Electron ◽  
Area Pellucida

Intrauterine eggs at different stages of development were retrieved from hens and the blastodiscs were dissected out and prepared for scanning electron microscopy. Our observations trace the polarized shedding of cells from the central area of the lower layers of the blastodisc, which leads to the formation of the area pellucida. Prior to the shedding, single central blebs appear on the ventral side of the cells facing the sub-blastodermic cavity. We also noted the presence of lobopodia which establish contact with nearby cells. The possible function of the lobopodia is discussed. The cells of the blastodisc are at the same time (stages IV and V, E.-G.&K). also intimately interconnected via a profusion of threadlike cytoplasmic processes (filopodia). When shedding starts the blebs as well as the lobopodia disappear, and the filopodia undergo typical withdrawal steps such as showing a beaded stage and appearing drawn out or taut, before they finally disrupt to leave behind a smooth round cell which is detached from its neighbours. The beading phenomenon points to the possibility of a disassembly of the microtubular skeleton of the filopodia operated by some physical force influenced by gravity.

Neogene occurrences of the marine acritarch genusNannobarbophoraHabib and Knapp, 1982 emend., and the new speciesN. Gedlii

2003 ◽  
Vol 77 (2) ◽  
pp. 382-385 ◽  
Author(s):  
Martin J. Head
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
North Atlantic ◽  
Diagnostic Feature ◽  
Sem Study ◽  
Scanning Electron

The stratigraphic utility of small marine acritarchs in the Neogene has been known for more than a decade (e.g., de Vernal and Mudie, 1989). Their potential for biostratigraphy in the Cretaceous is also well known, and was elegantly elucidated by Habib and Knapp (1982) in a detailed scanning electron microscope (SEM) study from the western North Atlantic. Habib and Knapp erected 12 new acritarch genera including the acanthomorph genusNannobarbophoraand its three speciesN. barbata(the name of the type),N. pistilla, andN. platforma. A diagnostic feature ofNannobarbophorais the presence of spinules concentrated on, or restricted to, the surface of distally closed processes. Excystment is by a simple linear split (Habib and Knapp, 1982, p. 347).

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