Study on Biometric Parameters and Genetic Diversity of Labeo rohita from Harike Wetland-A Ramsar Site

2020 ◽  
Author(s):  
Surjya Narayan Datta ◽  
Armaandeep Kaur ◽  
Anuj Tyagi
Keyword(s):  
Genetic Diversity ◽  
Labeo Rohita ◽  
Coi Gene ◽  
Series Data ◽  
Ramsar Site ◽  
Important Food ◽  
Commercially Important ◽  
Food Fish ◽  
Harike Wetland ◽  
Catch Composition

Background: Harike wetland (31°08¢ N to 31°23¢ N latitudes and 74°90¢ E to 75°12¢ E longitudes) is an internationally important Ramsar site, supports rare, vulnerable and endangered plants, fish and other faunal species. Weed infestation, pollution and encroachment are considered as important threats of its biota thus time series data with respect to fish diversity, catch composition and genetic variability has an utter importance to interpret changes over time. Cyprinidae family of freshwater fishes includes carps is the largest fish family found in Harike wetland comprising around 50% of total fish composition by weight basis and among carps, Rohu (Labeo rohita) is one of the commercially important food fish species available throughout the year and preferred by consumers. As river Beas and Sutlej, two major rivers of Indus river system confluence at Harike thus possibility of variation within fish stock is very high. With this background present study was carried out to evaluate the fish biodiversity and catch composition of Harike wetland. Study also emphasized on biometrics, length –weight relationship and genetic diversity based on mitochondrial marker genes of commercially important food fish Labeo rohita. Methods: Assessment of fish catch composition and diversity were conducted in landing centre adjacent to Harike wetland through Rapid Fisheries Assessment by Market Survey (RFAMS) technique. Fin tissue samples were collected for genetic diversity analysis of L. rohita by cytochrome oxidase subunit I (COI) gene sequencing, From the fish genomic DNA a partial fragment of approximately 655bp was PCR amplified by FishF1 (5'-TCAACCAACCACAAAGACATTGGCAC-3') and FishR1 (5'-TCGACTAATCATAAAGATATCGGC AC-3') primer pair. Calculation of intraspecific mean and pairwise distances was performed by MEGA 6.0 software using the K2P parameters Results: Total 30 species of fishes were recorded from Harike wetland and these belong to 14 families and 21 genera. In L. rohita average weight (Wt), total length (TL), standard length (SL) and forked length (FL) were recorded 2600±5.64g (1700-3600 g), 58.2±5.65 cm (51.3-67.6cm), 48.0±8.54 cm (42.5-55.5cm) and 46.0±0.25 cm (38.0-54.5cm), respectively. Biometric study revealed that sufficient numbers of mature L. rohita are available in wetland. L. rohita established negative algometric growth (b= 2.701); thus species became slender as it increased in length. The pairwise distances ranged from 0.00 to 18.49% with a mean ±S.E value of 4.70%±0.40. Total of 9 haplotypes were observed in L. rohita COI sequences. The diversity in haplotype and nucletide values were observed 0.848 and 0.024, respectively. The present study states that L. rohita stocks in Harike wetland are genetically diverse.

scholarly journals Length-weight relationships of the Indian Major Carp, Labeo rohita (Cypriniformes: Cyprinidae) from six drainages of Ganga basin, India

2015 ◽  
Vol 7 (1) ◽  
pp. 71-77
Author(s):  
Javaid Iqbal Mir ◽  
Uttam Kumar Sarkar ◽  
Om Prakash Gusain ◽  
Arvind Kumar Dwivedi
Keyword(s):  
Labeo Rohita ◽  
Main Channel ◽  
Coefficient Of Determination ◽  
Important Food ◽  
Indian Major Carp ◽  
Ganga Basin ◽  
Natural Range ◽  
Food Fish ◽  
Management And Conservation ◽  
Tropical Freshwater

The Indian Major Carp, Labeo rohita, is a geographically widespread and economically important food fish species in tropical freshwater of India and adjacent countries. We studied the lengthweightrelationships of 1 033 specimens collected from the main channel of Ganga river and its five major drainages from March 2009 to July 2012. The length of males ranged from 16 to 92cm (females: 16to 94cm). The growth is allometric positive (b>3) for males, females and pooled sexes. The coefficient of determination (r2) in males ranged from 0,978 to 0,989 and for females from 0,958 to 0,985. Data from field populations are scarce and our results will be useful in the management and conservation of L. rohita populations in its natural range.

Use by juvenile barramundi, Lates calcarifer (Bloch), and other fishes of temporary supralittoral habitats in a tropical estuary in Northern Australia

1983 ◽  
Vol 34 (5) ◽  
pp. 805 ◽  
Author(s):  
DJ Russell ◽  
RN Garrett
Keyword(s):  
Juvenile Fish ◽  
River Estuary ◽  
Temporary Pools ◽  
Tropical Estuary ◽  
Northern Australia ◽  
Tidal Inundation ◽  
Important Food ◽  
Lates Calcarifer ◽  
Commercially Important ◽  
Food Fish

Use by fishes of temporary pools created by high seasonal tides, which occur each year from October until April, on the saltpans and littorals adjacent to the Norman River estuary in northern Queensland was investigated. Fish entering the pools were sampled with one-way tidal traps placed in inlet gutters, and fish in the pools were poisoned using rotenone. Recruitment to the pools by fish occurred soon after the initial tidal inundation. Juvenile fish of 37 species, including the commercially important food fish L. calcarifer, used the pools. The first barramundi were found 8 weeks after initial inundation and for the remainder of the study they continually migrated into these habitats. The total length of the smallest L. calcarifer sampled was 9.5 mm. Salinities in pools containing fish ranged from 94 × 10-3 to less than 1 × 10-3 and temperatures reached 36�C.

Fish Erythrocytes as Biomarkers for the Toxicity of Sublethal Doses of an Azo Dye, Basic Violet-1 (CI: 42535)

2014 ◽  
Vol 21 (1) ◽  
pp. 264-273 ◽  
Author(s):  
Kirandeep Kaur ◽  
Arvinder Kaur
Keyword(s):  
Azo Dye ◽  
Labeo Rohita ◽  
Abnormal Behavior ◽  
Important Food ◽  
Short Term ◽  
Sem Study ◽  
Test Fish ◽  
Food Fish ◽  
Sublethal Doses ◽  
Scanning Electron

AbstractThe aim of the present study was to investigate poikilocytosis in Labeo rohita (an important food fish) as an early indicator of stress due to an azo dye, Basic Violet-1 (CI: 42535). This dye was observed to be very toxic to test fish (96 h LC50 as0.45 mg/L dye). Fish were given short-term (96 h) and subchronic (150 days) exposures to the dye, and poikilocytosis was recorded under light and scanning electron microscopy (SEM). Light microscopy helped in identification of micronuclei along with irregularities, notches, blebs, lobes, crenation, clumps, chains, spherocytes, vacuolation, and necrosis in erythrocytes. However, SEM indicated shrinkage, oozing of cytoplasm, and several new abnormal shapes including marginal foldings, discocytes, keratocytes, dacrocytes, degmacytes, acanthocytes, echinocytes, protuberances, stomatocytes, drepanocytes, holes in the membrane, stippling/spicules, crescent-shaped cells, triangular cells, and pentagonal cells. Earlier studies speculated changes in the membrane to be responsible for clumping and chaining of erythrocytes, whereas the present SEM study clearly indicates that oozing out of cytoplasm is also responsible for the formation of chains and clumps. This study also shows that erythrocytes exhibit pathological symptoms before the appearance of other external symptoms such as abnormal behavior or mortality of fish. There was a dose- and duration-dependent increase; therefore, poikilocytosis, especially echinocytes, spherocytes, and clumps, can act as a biomarker for the stress caused by azo dyes.

scholarly journals Phylogeography of the Brittle Star Ophiura sarsii Lütken, 1855 (Echinodermata: Ophiuroidea) from the Barents Sea and East Atlantic

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 40
Author(s):  
Evgeny Genelt-Yanovskiy ◽  
Yixuan Li ◽  
Ekaterina Stratanenko ◽  
Natalia Zhuravleva ◽  
Natalia Strelkova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Barents Sea ◽  
Haplotype Diversity ◽  
Brittle Star ◽  
The Arctic ◽  
Coi Gene ◽  
High Genetic Diversity ◽  
Svalbard Archipelago ◽  
Mitochondrial Coi ◽  
The Barents Sea

Ophiura sarsii is a common brittle star species across the Arctic and Sub-Arctic regions of the Atlantic and the Pacific oceans. Ophiurasarsii is among the dominant echinoderms in the Barents Sea. We studied the genetic diversity of O.sarsii by sequencing the 548 bp fragment of the mitochondrial COI gene. Ophiurasarsii demonstrated high genetic diversity in the Barents Sea. Both major Atlantic mtDNA lineages were present in the Barents Sea and were evenly distributed between the northern waters around Svalbard archipelago and the southern part near Murmansk coast of Kola Peninsula. Both regions, and other parts of the O.sarsii range, were characterized by high haplotype diversity with a significant number of private haplotypes being mostly satellites to the two dominant haplotypes, each belonging to a different mtDNA clade. Demographic analyses indicated that the demographic and spatial expansion of O.sarsii in the Barents Sea most plausibly has started in the Bølling–Allerød interstadial during the deglaciation of the western margin of the Barents Sea.

scholarly journals Status of formalin in commercially important fishes from the northeastern region of Bangladesh

2020 ◽  
Vol 32 (1) ◽  
pp. 23-28
Author(s):  
TOFAEL AHMED SUMON ◽  
MD. ASHRAF HUSSAIN ◽  
FARJANA AKHTER MITA ◽  
JOYANTA BIR ◽  
SARKER MOHAMMED IBRAHIM KHALIL
Keyword(s):  
Labeo Rohita ◽  
Industrial Research ◽  
Catla Catla ◽  
Northeastern Region ◽  
Significant Difference ◽  
Fish Samples ◽  
The Status ◽  
Commercially Important ◽  
Tenualosa Ilisha

The study revealed the status of formalin used in four commercially important fishes namely Rohu (Labeo rohita), Catla (Catla catla), Mrigal (Cirrhinus cirrhosus) and Hilsa (Tenualosa ilisha) from Sylhet and Moulvibazar district for a period of four months from April to July 2016. A total of 100 fish samples from different town and village markets were collected and analyzed instantly on the market to detect the presence of formalin by using a kit developed by Bangladesh Council of Scientific and Industrial Research (BCSIR). Among the analyzed fishes 46.7, 26.7, 20.0 and 13.3% of Rohu, Catla, Mrigal and Hilsa, respectively were found treated with formalin from Sylhet district. Similarly, formalin was also detected in 40, 20, 20 and 20% of investigated Rohu, Catla, Mrigal and Hilsa from Moulvibazar district, individually. There was no significant difference in formalin used between town and village market, whereas a significant difference in application of formalin in domestic and imported fishes except Catla has been identified. Two-third of imported Rohu (66.7%) was found formalin positive followed by Hilsa (44.4%), Mrigal (40%) and Catla (35.7%). Amongst the domestic fishes, presence of formalin was detected in 27.8, 18.8, 10.0 and 4.8% in Rohu, Catla, Mrigal and Hilsa, separately. This devastating use of formalin in food fishes should be banned through proper enactment of the existing laws of the country, as well as timely and appropriate implementation of government monitoring is mandatory to combat the unethical formalin use.

scholarly journals Genetic Diversity and Population Structure of Metaphire vulgaris Based on the Mitochondrial COI Gene and Microsatellites

2021 ◽  
Vol 12 ◽  
Author(s):  
Yu Fang ◽  
Jie Chen ◽  
Honghua Ruan ◽  
Nan Xu ◽  
Ziting Que ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Coi Gene ◽  
Small Scale ◽  
The Novel ◽  
Mitochondrial Coi ◽  
Allele Number ◽  
Medicinal Value ◽  
Mitochondrial Coi Gene ◽  
Data Content ◽  
Genetic Structures

The earthworm species Metaphire vulgaris (a member of the Clitellata class) is widely distributed across China, and has important ecological functions and medicinal value. However, investigations into its genetic diversity and differentiation are scarce. Consequently, we evaluated the genetic diversity of five populations of M. vulgaris (GM, HD, NYYZ, QDDY, and QDY) in Yancheng, China via the mitochondrial COI gene and the novel microsatellites developed there. A total of nine haplotypes were obtained by sequencing the mitochondrial COI gene, among which NYYZ and QDDY populations had the greatest number of haplotypes (nh = 5). Further, the nucleotide diversity ranged from 0.00437 to 0.1243. The neighbor-joining trees and the TCS network of haplotypes indicated that earthworm populations within close geographical range were not genetically isolated at these small scale distances. Results of the identification of microsatellite molecular markers revealed that the allele number in 12 microsatellite loci ranged from 4 to 13. The observed heterozygosity ranged from 0.151 to 0.644, whereas the expected heterozygosity ranged from 0.213 to 0.847. The polymorphism data content of most sites was >0.5, which indicated that the designed sites had high polymorphism. Structural analysis results indicated that GM, HD, and NYYZ had similar genetic structures across the five populations. The Nei’s genetic distance between HD and NYYZ populations was the smallest (Ds = 0.0624), whereas that between HD and QDY populations was the largest (Ds = 0.2364). The UPGMA tree showed that HD were initially grouped with NYYZ, followed by GM, and then with QDDY. Furthermore, cross-species amplification tests were conducted for Metaphire guillelmi, which indicated that the presented markers were usable for this species. This study comprised a preliminary study on the genetic diversity of M. vulgaris, which provides basic data for future investigations into this species.

scholarly journals DNA BARCODING USING COI GENE SEQUENCES OF WILD BETTA FIGHTING FISH FROM INDONESIA: PHYLOGENY, STATUS AND DIVERSITY

2020 ◽  
Vol 26 (2) ◽  
pp. 97
Author(s):  
Melta R. Fahmi ◽  
Eni Kusrini ◽  
Erma P. Hayuningtiyas ◽  
Shofihar Sinansari ◽  
Rudhy Gustiano
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Gene Sequence ◽  
Ornamental Fish ◽  
Coi Gene ◽  
Tree Construction ◽  
In The Wild ◽  
Blast Program

The wild betta fish is a potential ornamental fish export commodity normally caught by traders or hobbyists in the wild. However, the population of wild betta in nature has declined and become a threat for their sustainability. This research was conducted to analyze the genetic diversity, phylogenetic relationships, and molecular identification through DNA COI gene sequence of Indonesian wild betta fish. A total of 92 wild betta fish specimens were collected in this study. Amplification of COI genes was carried out using Fish F1, Fish R1, Fish F2, and Fish R2 primers. The genetic diversity and phylogenetic relationships were analyzed using MEGA version 5 software program. Species identification of the specimen was conducted using BLAST program with 98-100% similarity value of the DNA sequences to indicate the same species. Phylogenetic tree construction showed seven monophyletic clades and showed that Betta smaragdina was the ancestral species of genus Betta in Indonesian waters. Genetic distance among species ranged from 0.02 to 0.30, whereas intra-species genetic distance ranged from 0 to 6.54.

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