Surface-Enhanced Raman Spectroscopy for Bacterial Discrimination Utilizing a Scanning Electron Microscope with a Raman Spectroscopy Interface

2004 ◽  
Vol 76 (17) ◽  
pp. 5198-5202 ◽  
Author(s):  
Roger M. Jarvis ◽  
Alan Brooker ◽  
Royston Goodacre
Keyword(s):  
Raman Spectroscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Scanning Electron

Scanning Electron Microscopy and Surface Enhanced Raman Spectroscopy Correlation Studies of Functionalized Composite Organic-Inorganic SERS Nanoparticles on Cancer Cells

2011 ◽  
Vol 1316 ◽  
Author(s):  
Ai Leen Koh ◽  
Robert Sinclair
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Excitation Light ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Intensity ◽  
Scanning Electron

ABSTRACTComposite Organic-Inorganic Nanoparticles (COINs) are a novel type of surface-enhanced Raman (SER) scattering nanoparticle formed by aggregating inorganic silver particles in the presence of a chosen organic molecule with a distinct Raman fingerprint. Binding between antibody-functionalized COINs and cells is detected primarily using Raman spectroscopy, which measures spectral shifts of the excitation light due to inelastic scattering. It has been suggested that the amount of antibody-conjugated COINs binding on cells will vary according to the antigen-expression levels in cells and will lead to changes in measured SERS intensities. COINs functionalized with antibodies CD54 and CD8 were conjugated to U937 and SupT1 cancer cells and investigated in this study. SERS intensity measurements were obtained from each of the four sample variants and normalized against control samples comprising non-antibody-functionalized COINs with cells. The amount of COINs binding on cells was determined using scanning electron microscopy (SEM) and correlated with the SER spectroscopy intensity. Although we found a positive correlation between the number of COINs binding to cells and their respective SERS intensity, this relationship is not one-to-one, nor does it appear to be linear. We demonstrated that SEM imaging and SER spectroscopy can complement each other to provide information about COINs binding onto cancer cells.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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