Electrogenerated Chemiluminescence Peptide-Based Biosensor for the Determination of Prostate-Specific Antigen Based on Target-Induced Cleavage of Peptide

2014 ◽  
Vol 86 (3) ◽  
pp. 1372-1379 ◽  
Author(s):  
Honglan Qi ◽  
Min Li ◽  
Manman Dong ◽  
Sanpeng Ruan ◽  
Qiang Gao ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Electrogenerated Chemiluminescence

Reagent-less electrogenerated chemiluminescence peptide-based biosensor for the determination of prostate-specific antigen

Talanta ◽  
2012 ◽  
Vol 100 ◽  
pp. 162-167 ◽  
Author(s):  
Honglan Qi ◽  
Chen Wang ◽  
Xiaoying Qiu ◽  
Qiang Gao ◽  
Chengxiao Zhang
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Electrogenerated Chemiluminescence

scholarly journals Determination of Non-α1-Antichymotrypsin-complexed Prostate-specific Antigen as an Indirect Measurement of Free Prostate-specific Antigen: Analytical Performance and Diagnostic Accuracy

2003 ◽  
Vol 49 (6) ◽  
pp. 887-894 ◽  
Author(s):  
Sebastian Wesseling ◽  
Carsten Stephan ◽  
Axel Semjonow ◽  
Michael Lein ◽  
Brigitte Brux ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Sensitivity And Specificity ◽  
Specific Antigen ◽  
Roc Curves ◽  
Indirect Measurement ◽  
Free Psa ◽  
Specificity And Sensitivity ◽  
Diagnostic Sensitivity And Specificity ◽  
Total Psa

Abstract Background: A new assay measures prostate-specific antigen (PSA) not complexed to α1-antichymotrypsin (nACT-PSA) after removing PSA complexed to ACT by use of anti-ACT antibodies. We evaluated nACT-PSA and its ratio to total PSA (tPSA) as alternatives to free PSA (fPSA) and its ratio to tPSA in differentiating prostate cancer (PCa) and benign prostatic hyperplasia (BPH) in patients with tPSA of 2–20 μg/L. Methods: PSA in serum of 183 untreated patients with PCa and 132 patients with BPH was measured retrospectively on the chemiluminescence immunoassay analyzer LIAISON® (Byk-Sangtec Diagnostica) with the LIAISON tPSA and LIAISON fPSA assays. The nACT-PSA fraction was determined with a prototype assay measuring the residual PSA after precipitation of ACT-PSA with an ACT-precipitating reagent. Results:nACT-PSA was higher than fPSA in samples with fPSA concentrations <1 μg/L but lower in samples with >1 μg/L fPSA. The median ratios of fPSA/tPSA and of nACT-PSA/tPSA were significantly different between patients with BPH and PCa (19.4% vs 12.2% and 17.4% vs 13.0%, respectively). Within the tPSA ranges tested (2–20, 2–10, and 4–10 μg/L), areas under the ROC curves for the fPSA/tPSA ratios were significantly larger than those for nACT-PSA/tPSA. In the tPSA ranges <10 μg/L, the areas under the ROC curves for fPSA/tPSA were significantly larger than those for tPSA, whereas the areas for nACT-PSA/tPSA were not. At decision limits for 95% sensitivity and specificity, both ratios significantly increased specificity and sensitivity, respectively, compared with tPSA, but the fPSA/tPSA ratio showed higher values. Conclusions: nACT-PSA and its ratio to tPSA provide lower diagnostic sensitivity and specificity than fPSA/tPSA. The fPSA/tPSA ratio represents the state-of-the-art method for differentiating between PCa and BPH.

scholarly journals Simultaneous determination of free and total prostate-specific antigen by a magnetic particle-based time-resolved fluoroimmunoassay

2017 ◽  
Vol 31 (6) ◽  
pp. e22137 ◽  
Author(s):  
Bin Zhou ◽  
Jue Zhang ◽  
Zhongwei Lv ◽  
Jun Fan ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Prostate Specific Antigen ◽  
Magnetic Particle ◽  
Specific Antigen ◽  
Time Resolved ◽  
Total Prostate Specific Antigen

Determination of Prostate-Specific Antigen in Serum and a Reference Material by On-Chip Immunoaffinity Chromatography

2014 ◽  
Vol 47 (18) ◽  
pp. 2919-2928 ◽  
Author(s):  
Sanja Goč ◽  
Maja Kosanović ◽  
Snežana Golubović ◽  
Ljiljana Hajduković ◽  
Miroslava Janković
Keyword(s):  
Reference Material ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Immunoaffinity Chromatography ◽  
On Chip

Determination of mean population values of the prostate-specific antigen test after correcting for verification bias

2005 ◽  
Vol 23 (16_suppl) ◽  
pp. 6031-6031
Author(s):  
R. S. Punglia ◽  
A. V. D’Amico ◽  
W. J. Catalona ◽  
K. A. Roehl ◽  
K. M. Kuntz
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Test ◽  
Verification Bias ◽  
Prostate Specific Antigen Test

scholarly journals Biosensor Based on a Nanowire Field-effect Transistor for the Determination of Prostate Specific Antigen

2017 ◽  
Vol 27 ◽  
pp. 234-235 ◽  
Author(s):  
Maya Rubtsova ◽  
Galina Presnova ◽  
Denis Presnov ◽  
Vladimir Krupenin ◽  
Vitaly Grigorenko ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Specific Antigen ◽  
Effect Transistor
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