Integrated Structural Analysis of N-Glycans and Free Oligosaccharides Allows for a Quantitative Evaluation of ER Stress

We have developed porTable 950 keV/3.95 MeV X-band (9.3 GHz) electron linear accelerator (LINAC)-based X-ray sources and conducted onsite prestressed concrete (PC) bridge inspection in the last 10 years. A T-shaped PC girder bridge with a thickness of 200–400 mm and a box-shaped PC girder bridge with a thickness of 200–800 mm were tested. X-ray transmission images of flaws such as thinning, fray, and disconnection caused by corrosion of PC wires and unfilled grout were observed. A three-dimensional structural analysis was performed to estimate the reduction in the yield stress of the bridge. In this study, we attempted to evaluate the unfilled grout quantitatively because it is the main flaw that results in water filling and corrosion. In the measured X-ray images, we obtained gray values, which correspond to the X-ray attenuation coefficients of filled/unfilled grouts, PC wires (steel) in a sheath, and concrete. Then, we compared the ratio of the gray values of the filled/unfilled grouts and PC wires to determine the stage of the unfilled grout. We examined this quantitative evaluation using the data obtained from a real T-shaped PC girder bridge and model samples to simulate thick box-shaped PC girder bridges. We obtained a clear quantitative difference in the ratios for unfilled and filled grouts, which coincided with our visual perception. We synthesized the experience and data and proposed a quantitative analysis for evaluating the unfilled grout for subsequent steps such as structural analysis and destructive evaluation by boring surveys.

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Modern approaches to product competitiveness evaluation for companies of various industries

MATEC Web of Conferences ◽

10.1051/matecconf/201821602016 ◽

2018 ◽

Vol 216 ◽

pp. 02016

Author(s):

Elena Tyunyukova ◽

Vladimir Ruban ◽

Vladimir Burovtsev

Keyword(s):

Structural Analysis ◽

Quantitative Evaluation ◽

Quantitative Assessment ◽

Desirability Function ◽

Qualitative And Quantitative ◽

Relevant Task ◽

Economic Studies

This study is aimed at forming modern approaches to product competitiveness evaluation for companies of various industries. Relevant task is to obtain a quantitative evaluation parameter for product competitiveness that enables to adequately compare competing products. The study was performed using the methods of systems and structural analysis, strategic modeling and general principles of economic studies. The paper includes review of the existing approaches to product competitiveness evaluation based on its qualitative and quantitative assessment. Key groups of parameters that effect competitiveness of the manufactured product were determined, and an approach based on Harrington desirability function was suggested for its evaluation.

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Structural analysis of the surface-layer protein of spirillum serpens by high-resolution electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077268 ◽

1983 ◽

Vol 41 ◽

pp. 738-739

Author(s):

W. H. Wu ◽

R. M. Glaeser

Keyword(s):

Electron Microscopy ◽

Surface Layer ◽

Structural Analysis ◽

High Resolution ◽

Low Dose ◽

High Resolution Electron Microscopy ◽

Optical Diffraction ◽

Surface Layer Protein ◽

Morphological Unit ◽

Resolution Electron

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.

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RNA polymerase: Preparation and structural analysis of helical polymers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011091x ◽

1984 ◽

Vol 42 ◽

pp. 152-153

Author(s):

E. Loren Buhle ◽

Pamela Rew ◽

Ueli Aebi

Keyword(s):

Structural Analysis ◽

Rna Polymerase ◽

Molecular Level ◽

X Ray Diffraction ◽

Helical Polymers ◽

X Ray ◽

E Coli ◽

The Past ◽

Ordered Arrays ◽

Low Ionic Strength

While DNA-dependent RNA polymerase represents one of the key enzymes involved in transcription and ultimately in gene expression in procaryotic and eucaryotic cells, little progress has been made towards elucidation of its 3-D structure at the molecular level over the past few years. This is mainly because to date no 3-D crystals suitable for X-ray diffraction analysis have been obtained with this rather large (MW ~500 kd) multi-subunit (α2ββ'ζ). As an alternative, we have been trying to form ordered arrays of RNA polymerase from E. coli suitable for structural analysis in the electron microscope combined with image processing. Here we report about helical polymers induced from holoenzyme (α2ββ'ζ) at low ionic strength with 5-7 mM MnCl2 (see Fig. 1a). The presence of the ζ-subunit (MW 86 kd) is required to form these polymers, since the core enzyme (α2ββ') does fail to assemble into such structures under these conditions.

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Interpreting HVEM of muscle-cell impulse networks

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012103x ◽

1992 ◽

Vol 50 (1) ◽

pp. 126-127

Author(s):

Paul DeCosta ◽

Kyugon Cho ◽

Stephen Shemlon ◽

Heesung Jun ◽

Stanley M. Dunn

Keyword(s):

Structural Analysis ◽

Muscle Cell ◽

Electron Micrographs ◽

Relative Size ◽

Automatic Classification ◽

Nerve Fibers ◽

Analysis Algorithm ◽

Structural Networks

Introduction: The analysis and interpretation of electron micrographs of cells and tissues, often requires the accurate extraction of structural networks, which either provide immediate 2D or 3D information, or from which the desired information can be inferred. The images of these structures contain lines and/or curves whose orientation, lengths, and intersections characterize the overall network.Some examples exist of studies that have been done in the analysis of networks of natural structures. In, Sebok and Roemer determine the complexity of nerve structures in an EM formed slide. Here the number of nodes that exist in the image describes how dense nerve fibers are in a particular region of the skin. Hildith proposes a network structural analysis algorithm for the automatic classification of chromosome spreads (type, relative size and orientation).

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