Functional Characterization of Salicylic Acid Carboxyl Methyltransferase from Camellia sinensis, Providing the Aroma Compound of Methyl Salicylate during the Withering Process of White Tea

2017 ◽  
Vol 65 (50) ◽  
pp. 11036-11045 ◽  
Author(s):  
Wei-Wei Deng ◽  
Rongxiu Wang ◽  
Tianyuan Yang ◽  
Li’na Jiang ◽  
Zheng-Zhu Zhang
Keyword(s):  
Salicylic Acid ◽  
Camellia Sinensis ◽  
Methyl Salicylate ◽  
Functional Characterization ◽  
Aroma Compound ◽  
White Tea ◽  
Carboxyl Methyltransferase

Functional Characterization of a New Tea (Camellia sinensis) Flavonoid Glycosyltransferase

2017 ◽  
Vol 65 (10) ◽  
pp. 2074-2083 ◽  
Author(s):  
Xianqian Zhao ◽  
Peiqiang Wang ◽  
Mingzhuo Li ◽  
Yeru Wang ◽  
Xiaolan Jiang ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Functional Characterization

scholarly journals Establishing a System for Functional Characterization of Full-Length cDNAs of Camellia sinensis

2019 ◽  
Vol 20 (23) ◽  
pp. 5929
Author(s):  
Lin ◽  
Cai ◽  
Du ◽  
Zhang ◽  
Xu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Heterologous Expression ◽  
Cdna Library ◽  
Camellia Sinensis ◽  
Functional Characterization ◽  
Full Length ◽  
Rare Gene ◽  
Cdna Collection ◽  
Reference Genomes

: Tea (Camellia sinensis) is enriched with bioactive secondary metabolites, and is one of the most popular nonalcoholic beverages globally. Two tea reference genomes have been reported; however, the functional analysis of tea genes has lagged, mainly due to tea’s recalcitrance to genetic transformation and the absence of alternative high throughput heterologous expression systems. A full-length cDNA collection with a streamlined cloning system is needed in this economically important woody crop species. RNAs were isolated from nine different vegetative tea tissues, pooled, then used to construct a normalized full-length cDNA library. The titer of unamplified and amplified cDNA library was 6.89 × 106 and 1.8 × 1010 cfu/mL, respectively; the library recombinant rate was 87.2%. Preliminary characterization demonstrated that this collection can complement existing tea reference genomes and facilitate rare gene discovery. In addition, to streamline tea cDNA cloning and functional analysis, a binary vector (pBIG2113SF) was reengineered, seven tea cDNAs isolated from this library were successfully cloned into this vector, then transformed into Arabidopsis. One FL-cDNA, which encodes a putative P1B-type ATPase 5 (CsHMA5), was characterized further as a proof of concept. We demonstrated that overexpression of CsHMA5 in Arabidopsis resulted in copper hyposensitivity. Thus, our data demonstrated that this represents an efficient system for rare gene discovery and functional characterization of tea genes. The integration of a tea FL-cDNA collection with efficient cloning and a heterologous expression system would facilitate functional annotation and characterization of tea genes.

scholarly journals Characterization of Solanum melongena Thioesterases Related to Tomato Methylketone Synthase 2

Genes ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 549 ◽  
Author(s):  
Khuat ◽  
Bui ◽  
Tran ◽  
Truong ◽  
Nguyen ◽  
...  
Keyword(s):  
Methyl Salicylate ◽  
Functional Characterization ◽  
Solanum Melongena ◽  
Industrial Applications ◽  
Biofuel Production ◽  
Mechanical Wounding ◽  
Acyl Carrier Proteins ◽  
E Coli ◽  
High Level

2-Methylketones are involved in plant defense and fragrance and have industrial applications as flavor additives and for biofuel production. We isolated three genes from the crop plant Solanum melongena (eggplant) and investigated these as candidates for methylketone production. The wild tomato methylketone synthase 2 (ShMKS2), which hydrolyzes β-ketoacyl-acyl carrier proteins (ACP) to release β-ketoacids in the penultimate step of methylketone synthesis, was used as a query to identify three homologs from S. melongena: SmMKS2-1, SmMKS2-2, and SmMKS2-3. Expression and functional characterization of SmMKS2s in E. coli showed that SmMKS2-1 and SmMKS2-2 exhibited the thioesterase activity against different β-ketoacyl-ACP substrates to generate the corresponding saturated and unsaturated β-ketoacids, which can undergo decarboxylation to form their respective 2-methylketone products, whereas SmMKS2-3 showed no activity. SmMKS2-1 was expressed at high level in leaves, stems, roots, flowers, and fruits, whereas expression of SmMKS2-2 and SmMKS2-3 was mainly in flowers and fruits, respectively. Expression of SmMKS2-1 was induced in leaves by mechanical wounding, and by methyl jasmonate or methyl salicylate, but SmMKS2-2 and SmMKS2-3 genes were not induced. SmMKS2-1 is a candidate for methylketone-based defense in eggplant, and both SmMKS2-1 and SmMKS2-2 are novel MKS2 enzymes for biosynthesis of methylketones as feedstocks to biofuel production.

Functional characterization of three flavonol synthase genes from Camellia sinensis: Roles in flavonol accumulation

Plant Science ◽  
2020 ◽  
Vol 300 ◽  
pp. 110632
Author(s):  
Xiaolan Jiang ◽  
Yufeng Shi ◽  
Zhouping Fu ◽  
Wei-Wei Li ◽  
Sanyan Lai ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Functional Characterization ◽  
Flavonol Synthase

scholarly journals Evolutionary and functional characterization of leucoanthocyanidin reductases from Camellia sinensis

Planta ◽  
2017 ◽  
Vol 247 (1) ◽  
pp. 139-154 ◽  
Author(s):  
Peiqiang Wang ◽  
Lingjie Zhang ◽  
Xiaolan Jiang ◽  
Xinlong Dai ◽  
Lijuan Xu ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Functional Characterization

scholarly journals Structural and functional insights into the LBD family involved in abiotic stress and flavonoid synthases in Camellia sinensis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xueying Zhang ◽  
Yuqing He ◽  
Wenda He ◽  
Hui Su ◽  
Yuefei Wang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Camellia Sinensis ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Flavonoid Biosynthesis ◽  
Gene Promoters ◽  
Rna Seq ◽  
Lateral Organ ◽  
Insight Into

Abstract Lateral organ boundaries domain (LBD) proteins are plant-specific transcription factors that play a crucial role in growth and development, as well as metabolic processes. However, knowledge of the function of LBD proteins in Camellia sinensis is limited, and no systematic investigations of the LBD family have been reported. In this study, we identified 54 LBD genes in Camellia sinensis. The expression patterns of CsLBDs in different tissues and their transcription responses to exogenous hormones and abiotic stress were determined by RNA-seq, which showed that CsLBDs may have diverse functions. Analysis of the structural gene promoters revealed that the promoters of CsC4H, CsDFR and CsUGT84A, the structural genes involved in flavonoid biosynthesis, contained LBD recognition binding sites. The integrative analysis of CsLBD expression levels and metabolite accumulation also suggested that CsLBDs are involved in the regulation of flavonoid synthesis. Among them, CsLOB_3, CsLBD36_2 and CsLBD41_2, localized in the nucleus, were selected for functional characterization. Yeast two-hybrid assays revealed that CsLBD36_2 and CsLBD41_2 have self-activation activities, and CsLOB_3 and CsLBD36_2 can directly bind to the cis-element and significantly increase the activity of the CsC4H, CsDFR and CsUGT84A promoter. Our results present a comprehensive characterization of the 54 CsLBDs in Camellia sinensis and provide new insight into the important role that CsLBDs play in abiotic and flavonoid biosynthesis.

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