A Tailor-Made Self-Sufficient Whole-Cell Biocatalyst Enables Scalable Enantioselective Synthesis of (R)-3-Quinuclidinol in a High Space-Time Yield

Anatase-free titanium silicalite-1 (TS-1) zeolite with high framework titanium content is highly required for catalysing selective oxidation reactions, while its synthesis generally suffers from cost, efficiency and environmental issues. Herein,...

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Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism

Microorganisms ◽

10.3390/microorganisms8111843 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1843

Author(s):

Sebastian Beblawy ◽

Laura-Alina Philipp ◽

Johannes Gescher

Keyword(s):

Escherichia Coli ◽

Electron Transfer ◽

Point Mutations ◽

Selective Adaptation ◽

Space Time ◽

Transport Chain ◽

Whole Cell Biocatalyst ◽

Genes Encoding ◽

Space Time Yield ◽

Respiratory Conditions

Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with an oxidation state independent from the substrate. The whole cell biocatalyst transfers the surplus of electrons to an electrode that can be used as a non-depletable electron acceptor. So far, anode-assisted fermentations were shown to provide high carbon efficiencies but low space-time yields. This study aimed at increasing space-time yields of an Escherichia coli-based anode-assisted fermentation of glucose to acetoin. The experiments build on an obligate respiratory strain, that was advanced using selective adaptation and targeted strain development. Several transfers under respiratory conditions led to point mutations in the pfl, aceF and rpoC gene. These mutations increased anoxic growth by three-fold. Furthermore, overexpression of genes encoding a synthetic electron transport chain to methylene blue increased the electron transfer rate by 2.45-fold. Overall, these measures and a medium optimization increased the space-time yield in an electrode-assisted fermentation by 3.6-fold.

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Efficient synthesis of Ibrutinib chiral intermediate in high space-time yield by recombinant E. coli co-expressing alcohol dehydrogenase and glucose dehydrogenase

RSC Advances ◽

10.1039/c8ra08100j ◽

2019 ◽

Vol 9 (4) ◽

pp. 2325-2331 ◽

Cited By ~ 1

Author(s):

Yitong Chen ◽

Baodi Ma ◽

Songshuang Cao ◽

Xiaomei Wu ◽

Yi Xu

Keyword(s):

Alcohol Dehydrogenase ◽

Glucose Dehydrogenase ◽

Space Time ◽

Optically Active ◽

Efficient Synthesis ◽

E Coli ◽

Efficient Process ◽

High Space ◽

Space Time Yield

A simple and efficient process for the synthesis of optically active (S)-N-boc-3-hydroxy piperidine was developed using the “designer cells” co-expressing alcohol dehydrogenase and glucose dehydrogenase.

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Characterization of a newly synthesized carbonyl reductase and construction of a biocatalytic process for the synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate with high space-time yield

Applied Microbiology and Biotechnology ◽

10.1007/s00253-013-5042-3 ◽

2013 ◽

Vol 98 (4) ◽

pp. 1671-1680 ◽

Cited By ~ 19

Author(s):

Zhong-Yu You ◽

Zhi-Qiang Liu ◽

Yu-Guo Zheng

Keyword(s):

Space Time ◽

Carbonyl Reductase ◽

High Space ◽

Space Time Yield ◽

Biocatalytic Process

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Production of cellulase by Microbulbifer hydrolyticus through co-fermentation of glucose and xylose from lignocellulose

BioResources ◽

10.15376/biores.15.4.8689-8695 ◽

2020 ◽

Vol 15 (4) ◽

pp. 8689-8695

Author(s):

Huan Liu ◽

Xiaolan Huang ◽

Qi Xiao ◽

Yue Yu ◽

Li Deng ◽

...

Keyword(s):

Growth Rate ◽

Marine Bacterium ◽

Cellulase Activity ◽

Cellulase Production ◽

Space Time ◽

Processing Industry ◽

Distiller's Grains ◽

High Space ◽

Space Time Yield ◽

Fast Growth Rate

Cellulase is a compound enzyme that catalyzes cellulose into monosaccharides or oligosaccharides. Large amounts of cellulase are needed with the development of the lignocellulose processing industry, which necessitates faster methods to produce cellulase. In this work, the marine bacterium Microbulbifer hydrolyticus IRE-31-192 was selected to produce cellulase, due to its fast growth rate and short high space-time yield. Co-fermentation of glucose and xylose to produce cellulase was investigated on the basis of previous work. When the ratio of glucose/xylose was 2:1 (w/w), 294 U/L cellulase activity with highest space-time yield of 12.2 U/L h was obtained. The hydrolytic liquid of lignocellulose prepared from dried distiller’s grains with solubles (DDGS) with the similar ratio of glucose/xylose was used as medium to produce cellulase. The efficiency of cellulase production from processed and unprocessed hydrolysates of DDGS was compared. Unprocessed hydrolysates were more beneficial for the production of cellulase, such that its activity was 261 U/L with a space-time yield of 14.5 U/L h. Thus, commonly used pure glucose and xylose could be replaced by hydrolysates of DDGS, and marine bacteria has potential application for cellulase production.

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