Adsorption Characteristics and Molecular Simulation of Malachite Green onto Modified Distillers’ Grains

Adsorbent material was prepared using distillers’ grains (DG), which is a waste product of distilleries. The DG was pre-treated with NaOH and esterification-modified with CS2, which is a commonly used anionic modifier. The structure and morphology of the adsorbent was characterized by FTIR, XRD, EDS, SEM, BET, and zeta potential. The related mechanism of adsorption of malachite green (MG) onto modified distiller’s grains (MDG) was studied by adsorption experiments and molecular simulation techniques. The experimental results showed that CS2 successfully modified the DG fiber, and simultaneously yielded the MDG with a uniform pore distribution. MDG had a considerable adsorption capacity of 367.39 mg/g and a maximum removal rate of 96.51%. After eight adsorption–desorption cycle experiments, the adsorption removal rate of MDG to MG dye remained at 82.6%. The adsorption process could be fitted well by a pseudo-second-order kinetic model (the correlation coefficient R2 > 0.998) and Freundlich isotherm adsorption equation (the correlation coefficient R2 > 0.972). Moreover, the adsorption of MG dye by MDG is a spontaneous, endothermic, and increased entropy process. The results of molecular simulation showed that the mechanism of MG molecules onto MDG was mainly chemical adsorption. The adsorption performance of MG onto MDG was better and more stable than DG. Molecular simulation also provided a theoretical guidance of MDG adsorption–desorption for the research on recycling of DG resources.

Study for Whitening Effect, Antioxidant and Anti-wrinkle Activity of Tapioca-unhulled Barley Dried Distiller's Grains with Solubles

This study intends to present the value of use as an eco-friendly, bioactive functional material by utilizing the undervalued tapiocaunhulled barley dried distiller's grains with solubles (TBDDGS). The physiological activity of TBDDGS presented through whitening activity, antioxidant activity, and anti-wrinkle effect. As a result of measuring the mushroom tyrosinase inhibitory activity in the hot water extracts of TBDDGS, the extracts showed 1.21% at 20 mg/mL concentration, 7.39% at 50 mg/mL concentration, and 25.78% at 100 mg/mL concentration, depending on the concentration of the extracts. The radical scavenging ability of DPPH was 28.7% at 10 mg/mL concentration, 38.0% at 20 mg/mL concentration, 60.9% at 50 mg/mL concentration, and 80.1% at 100 mg/mL concentration. The collagenase inhibitory activity of the extracts was 92.8% at a 6 mg/mL concentration. Elastase inhibitory activity was 97.8% at 100 mg/mL concentration. From the above results, the collagenase inhibitory activity of the extracts was 92.8% at a 6 mg/mL concentration. The elastase inhibitory activity was 97.8% at 100 mg/mL concentration. Although TBDDGS in this study has lower physiological activity compared to the control group, it was considered to have industrial value as a functional cosmetic raw material in consideration of the advantages of stable raw material supply and price competitiveness as an eco-friendly cosmetic raw material.

POSSIBILITIES OF DRIED DISTILLERS’ GRAINS WITH SOLUBLES APPLICATION IN FEED MIXTURES

The possibility of utilization of dried distiller’s grains with solubles (DDGS) in feed mixtures for feed production was investigated. Samples of maize hybrids ZP 548 and ZP 655b and DDGS obtained from hybrids ZP 548 and ZP 548c were used as components of the mixtures.The total protein content ranged from 12.42 % to 31.18 %, moisture from 5.49 % to 9.55 and ash content of 1.85 % to 4.37 %. The contents of NDF, ADF, ADL, hemicellulose and cellulose fibers ranged from: 13.90 % to 48.13 %; 2.96 % to 20.69 %; 0.27 % to 2.44 %; 10.69 % to 30.17 %, 2.62 % to 18.32 %, respectively. In vitro dry matter digestibility ranged from 55.20 % to 89.76 %. It was concluded that the samples of DDG obtained from red and yellow maize hybrids kernel are very suitable as components for the preparation of animal feed mixtures.

A Dilute-and-Shoot UHPLC-MS/MS Isotope Dilution Method for Simultaneous Determination and Confirmation of Eleven Mycotoxins in Dried Distillers Grains with Solubles

Background Natural contamination of mycotoxins in dried distiller’s grains with solubles (DDGS) as a mainstream animal feed ingredient poses risk to animal health. Objective A regulatory method was needed for the agency to simultaneously detect eleven mycotoxins of high regulatory priority in DDGS. Methods Ten grams of DDGS sample were extracted twice with acetonitrile/water under mildly acidic condition. Two aliquots from the combined crude extract were taken and processed separately: (1) diluted 400-fold with solvent for analysis of deoxynivalenol and fumonisins B1 and B2; (2) pH adjusted to 7.5, then diluted 15.7-fold for analysis of aflatoxins B1, B2, G1, G2, ochratoxin A, zearalenone, and T-2 and HT-2 toxins. Uniformly-labelled 13C-isotopologues of these mycotoxins were added as internal standards to the diluted extracts for quantitative analysis by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS). Results. The linear quantitation ranges (µg/kg) were: aflatoxin B1, B2, G1, and G2, 1.57 to 105; zearalenone, 16.3 to 1090; T-2 toxin, 3.14 to 208; HT-2 toxin, 48.2 to 3220; ochratoxin A, 0.47 to 31.4; deoxynivalenol, 240 to 16000; fumonisin B1 and B2, 320 to 21200. Accuracies for these analytes at each of three fortification levels range from 70.7% to 100%, with corresponding relative standard deviations between 1.4% to 10.5%. True recoveries were all higher than 83%. Conclusions This method was successfully validated to meet the agency’s performance guidelines for regulatory methods. Highlights This method is easy, quick and robust to simultaneously quantify and confirm presence of eleven regulated mycotoxins in DDGS.

Barrier Properties and Hydrophobicity of Biodegradable Poly(lactic acid) Composites Reinforced with Recycled Chinese Spirits Distiller’s Grains

Adding natural biomass to poly(lactic acid) (PLA) as a reinforcing filler is a way to change the properties of PLA. This paper is about preparing PLA/biomass composites by physically melting and blending Chinese Spirits distiller’s grains (CSDG) biomass and PLA to optimize the composite performance. Composites of modified PLA (MPLA) with varying amounts of CSDG were also prepared by the melt-mixing method, and unmodified PLA/CSDG composites were used as a control group for comparative analysis. The functional groups of MPLA enhanced the compatibility between the polymer substrate and CSDG. The composite water vapor/oxygen barrier and mechanical properties were studied. It was found that the barrier and mechanical properties of MPLA/CSDG composites were significantly improved. SEM was adopted to examine the tensile section structure of the composites, and the compatibility between the filler and the matrix was analyzed. An appropriate amount of CSDG had a better dispersibility in the matrix, and it further improved the interfacial bonding force, which in turn improved the composite mechanical properties. X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry were conducted to determine the crystalline properties and to analyze the stability of the composites. It was found that the CSDG content had a significant effect on the crystallinity. Barrier and biodegradation mechanisms were also discussed.

257 Including Sweet Bran in the Diet of Growing Feedlot Steers Increased Body Weight and Influenced Rate of Feed Disappearance

This study assessed the effect of Sweet BranTM (SWBR; 40% DM inclusion; Cargill Corn Milling, Blair, NE) or modified distiller’s grains (MDGS; 25% DM inclusion) in the diet of newly received feedlot steers on feeding behavior and growing period performance. Diets were formulated for similar metabolizable protein and DM. Two hundred sixteen freshly weaned Angus-cross steers (253 kg ± 18.1) were stratified by BW to 36 pens (n = 18 per treatment; 6 steers/pen). Individual BW were recorded on d 0, 28, and 60. Eight pens per treatment were utilized to assess rate of feed disappearance early (day 5 and 6) and late (53 and 54) during growing by weighing feed in bunks every 2 hours post-delivery for 12 consecutive hours. Feed weights were averaged across consecutive days within pen and timepoint. Slopes were calculated between each timepoint to determine rate of feed disappearance. Data were analyzed with Proc Mixed of SAS 9.4; diet was fixed effect and pen was experimental unit. Feed disappearance slopes were analyzed as repeated measures with the repeated effect of time. Body weights did not differ on d 28 (P ≥ 0.29), but d 60 BW and d 0 to 60 ADG were greater for SWBR than MDGS (P < 0.05). Steers fed SWBR had greater DMI throughout the trial (P < 0.05). Feed efficiency (G:F) did not differ throughout the trial (P ≥ 0.31). Rate of feed disappearance on d 5/6 was greater for SWBR between hours 6–8, and greater for MDGS from hours 10–12. On d 53/54, SWBR had increased feeding rate during hours 0–2, while MDGS was increased from hours 8–10. Steers fed SWBR had a greater rate of feed disappearance earlier in the day and had improved DMI and growth than steers fed MDGS.