Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications

2021 ◽  
Vol 4 (3) ◽  
pp. 2514-2522
Author(s):  
Odair Bim-Júnior ◽  
Fabiana Curylofo-Zotti ◽  
Mariana Reis ◽  
Yvette Alania ◽  
Paulo N. Lisboa-Filho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Collagen Scaffolds ◽  
Engineering Applications

Effects of Mechanical Stress on the In Vitro Degradation of Porous Composite Scaffold for Bone Tissue Engineering

2007 ◽  
Vol 342-343 ◽  
pp. 273-276 ◽  
Author(s):  
Yun Qing Kang ◽  
Guang Fu Yin ◽  
Lin Luo ◽  
Ke Feng Wang ◽  
Yu Zhang
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid ◽  
Bone Tissue Engineering ◽  
Mechanical Stress ◽  
Mechanical Loading ◽  
Body Fluid ◽  
Compressive Loading ◽  
Porous Scaffolds ◽  
In Vitro Degradation

In bone tissue engineering, porous scaffolds served as the temporary matrix are often subjected to mechanical stress when implanted in the body. Based on this fact, the goal of this study was to examine the effects of mechanical loading on the in vitro degradation characteristics and kinetics of porous scaffolds in a custom-designed loading system. Porous Poly(L-lactic acid)/β-Tricalcium Phosphate (PLLA/β-TCP) composite scaffolds fabricated by using solution casting/compression molding/particulate leaching technique (SCP) were subjected to degradation in simulated body fluid (SBF) at 37°C for up to 6 weeks under the conditions: with and without static compressive loading, respectively. The results indicated that the increase of the porosity and decrease of the compressive strength under static compressive loading were slower than that of non-loading case, and so did the mass loss rate. It might be due to that the loading retarded the penetration, absorption and transfer of simulated body fluid. These data provide an important step towards understanding mechanical loading factors contributing to degradation.

Multifarious Fabrication Approaches of Producing Aligned Collagen Scaffolds for Tissue Engineering Applications

2020 ◽  
Vol 6 (2) ◽  
pp. 779-797 ◽  
Author(s):  
Ankush Dewle ◽  
Navanit Pathak ◽  
Prakash Rakshasmare ◽  
Akshay Srivastava
Keyword(s):  
Tissue Engineering ◽  
Collagen Scaffolds ◽  
Engineering Applications

Evaluation of the in vitro biodegradation and biological behavior of poly(lactic-co-glycolic acid)/nano-fluorhydroxyapatite composite microsphere-sintered scaffold for bone tissue engineering

2017 ◽  
Vol 33 (2) ◽  
pp. 146-159 ◽  
Author(s):  
Mohammadreza Tahriri ◽  
Fathollah Moztarzadeh ◽  
Arash Tahriri ◽  
Hossein Eslami ◽  
Kimia Khoshroo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Weight Loss ◽  
Simulated Body Fluid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Body Fluid ◽  
Bone Repair ◽  
Glycolic Acid ◽  
Composite Scaffold

The objective of this research was to study the degradation and biological characteristics of the three-dimensional porous composite scaffold made of poly(lactic- co-glycolic acid)/nano-fluorhydroxyapatite microsphere using sintering method for potential bone tissue engineering. Our previous experimental results demonstrated that poly(lactic- co-glycolic acid)/nano-fluorhydroxyapatite composite scaffold with a ratio of 4:1 sintered at 90ºC for 2 h has the greatest mechanical properties and a proper pore structure for bone repair applications. The weight loss percentage of both poly(lactic- co-glycolic acid)/nano-fluorhydroxyapatite and poly(lactic- co-glycolic acid) scaffolds demonstrated a monotonic trend with increasing degradation time, that is, the incorporation of nano-fluorhydroxyapatite into polymeric scaffold could lead to weight loss in comparison with that of pure poly(lactic- co-glycolic acid). The pH change for composite scaffolds showed that there was a slight decrease until 2 weeks after immersion in simulated body fluid, followed by a significant increase in the pH of simulated body fluid without a scaffold at the end of immersion time. The mechanical properties of composite scaffold were higher than that of poly(lactic- co-glycolic acid) scaffold at total time of incubation in simulated body fluid; however, it should be noted that the incorporation of nano-fluorhydroxyapatite into composite scaffold leads to decline in the relatively significant mechanical strength and modulus during hydrolytic degradation. In addition, MTT assay and alkaline phosphatase activity results defined that a general trend of increasing cell viability was seen for poly(lactic- co-glycolic acid)/nano-fluorhydroxyapatite scaffold sintered by time when compared to control group. Eventually, experimental results exhibited poly(lactic- co-glycolic acid)/nano-fluorhydroxyapatite microsphere-sintered scaffold is a promising scaffold for bone repair.

Gelatin Coated 45S5 Bioglass®-Derived Scaffolds for Bone Tissue Engineering

2013 ◽  
Vol 541 ◽  
pp. 31-39 ◽  
Author(s):  
Anke Lisa Metze ◽  
Alexandra Grimm ◽  
Patcharakamon Nooeaid ◽  
Judith A. Roether ◽  
Jasmin Hum ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Compressive Strength ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Gelatin Layer ◽  
Gelatin Concentration ◽  
Gelatin Coating ◽  
45S5 Bioglass ◽  
Highly Porous

Highly porous 45S5 Bioglass® scaffolds were fabricated by the foam replica method and successfully coated with a well attached gelatin layer by dipping and pipetting methods. Depending on macropore size of the scaffold and gelatin concentration, mechanically enhanced scaffolds with improved compressive strength in comparison to uncoated scaffolds could be obtained while preserving the high and interconnected porosity that is required for bone in-growth. Moreover, the scaffolds bioactivity by immersion in simulated body fluid (SBF) was investigated showing that gelatin coating preserves the intrinsic bioactivity of the Bioglass® scaffold. It was also shown that the gelatin layer can be loaded with tetracycline hydrochloride for developing scaffolds with drug delivery capability.

Recent Development in the Fabrication of Collagen Scaffolds for Tissue Engineering Applications: A Review

2019 ◽  
Vol 20 (12) ◽  
pp. 992-1003 ◽  
Author(s):  
Mohammad F. Mh Busra ◽  
Yogeswaran Lokanathan
Keyword(s):  
Tissue Engineering ◽  
Ex Vivo ◽  
Physicochemical Characteristics ◽  
Collagen Scaffolds ◽  
Engineering Applications ◽  
Hydrogel Beads ◽  
Structural Support ◽  
Main Components ◽  
Sheet Membrane

Tissue engineering focuses on developing biological substitutes to restore, maintain or improve tissue functions. The three main components of its application are scaffold, cell and growthstimulating signals. Scaffolds composed of biomaterials mainly function as the structural support for ex vivo cells to attach and proliferate. They also provide physical, mechanical and biochemical cues for the differentiation of cells before transferring to the in vivo site. Collagen has been long used in various clinical applications, including drug delivery. The wide usage of collagen in the clinical field can be attributed to its abundance in nature, biocompatibility, low antigenicity and biodegradability. In addition, the high tensile strength and fibril-forming ability of collagen enable its fabrication into various forms, such as sheet/membrane, sponge, hydrogel, beads, nanofibre and nanoparticle, and as a coating material. The wide option of fabrication technology together with the excellent biological and physicochemical characteristics of collagen has stimulated the use of collagen scaffolds in various tissue engineering applications. This review describes the fabrication methods used to produce various forms of scaffolds used in tissue engineering applications.

Preparation and Characterization of Polylactide-co-glycolide/Carbonate Apatite (PLGA/CHA) Composite Scaffolds

2011 ◽  
Vol 493-494 ◽  
pp. 572-576
Author(s):  
Heather Elizabeth Stone ◽  
Helen Lu ◽  
Racquel Z. LeGeros
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Elastic Modulus ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Bone Repair ◽  
Carbonate Apatite ◽  
Composite Scaffolds ◽  
Physico Chemical

Both natural and synthetic materials have been utilized to provide three dimensional scaffold environments ideal for bone repair. The biomechanical and biocompatibility characteristics of these scaffolds play a vital role in successful tissue engineering constructs. Polymer/carbonate apatite (CHA) composites have shown to improve cell adhesion and proliferation on the scaffold as well as increase elastic modulus, toughness and strength. The aim of this study is to prepare CHA- polylactic-co-glycolide (PLGA) composites in the form of microsphere, scaffold and disc and evaluate their physico-chemical properties, mechanical properties and in vitro bioactivity. 3-D porous cylindrical composite scaffolds were prepared using PLGA/CHA composites with varying PLGA/CHA ratios (30:70 and 50:50). The CHA was prepared by hydrolysis method and characterized using x-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR). The physico-chemical and mechanical properties of the composite scaffolds were evaluated using scanning electron microscopy (SEM), micro-computed tomography (μCT), XRD, FTIR, and thermogravimetry (TGA). Flexural strength was determined using Instron. In vitro bioactivity was determined by the formation of apatite on composite disc surfaces after immersion in simulated body fluid (SBF). SEM and μCT analyses showed high porosity and interconnectivity between microspheres in the composite scaffolds. In vitro bioactivity was observed by the development of an apatite layer on the surfaces of the composite scaffolds after immersion in simulated body fluid. The mechanical strength of the scaffolds was to be dependent on the PLGA-CHA ratio. The elastic modulus, toughness and strength values obtained for the composites were similar to those of reported bone substituted materials. Results from this study provided information on the fabrication of PLGA-CHA scaffolds and their properties that may be useful for their potential application in bone repair and as scaffolds in tissue engineering for bone regeneration.

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