Large-Scale and Rapid Preparation of Nanofibrous Meshes and Their Application for Drug-Loaded Multilayer Mucoadhesive Patch Fabrication for Mouth Ulcer Treatment

ABSTRACTThrough the increase in the capacity of sequencing machines massively parallel sequencing of thousands of samples in a single run is now possible. With the improved throughput and resulting drop in the price of sequencing, the cost and time for preparation of sequencing libraries have become the major bottleneck in large-scale experiments. Methods using a hyperactive variant of the Tn5 transposase efficiently generate libraries starting from cDNA or genomic DNA in a few hours and are highly scalable. For genome sequencing, however, the time and effort spent on genomic DNA isolation limits the practicability of sequencing large numbers of samples. Here, we describe a highly scalable method for preparing high quality whole-genome sequencing libraries directly from yeast cultures in less than three hours at 34 cents per sample. We skip the rate-limiting step of genomic DNA extraction by directly tagmenting yeast spheroplasts and add a nucleosome release step prior to enrichment PCR to improve the evenness of genomic coverage. Resulting libraries do not show any GC-bias and are comparable in quality to libraries processed from genomic DNA with a commercially available Tn5-based kit. We use our protocol to investigate CRISPR/Cas9 on- and off-target edits and reliably detect edited variants and shared polymorphisms between strains. Our protocol enables rapid preparation of unbiased and high-quality, sequencing-ready indexed libraries for hundreds of yeast strains in a single day at a low price. By adjusting individual steps of our workflow we expect that our protocol can be adapted to other organisms.

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Large scale, rapid preparation of functional mesophyll chloroplasts from Zea mays and other C4 species

Plant Science Letters ◽

10.1016/0304-4211(84)90152-4 ◽

1984 ◽

Vol 35 (1) ◽

pp. 19-24 ◽

Cited By ~ 34

Author(s):

C.L.D. Jenkins ◽

V.J. Russ

Keyword(s):

Zea Mays ◽

Large Scale ◽

Rapid Preparation ◽

C4 Species

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Rapid Preparation of YBCO Coated Conductor by Chemical Solution Deposition

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.727.991 ◽

2017 ◽

Vol 727 ◽

pp. 991-995

Author(s):

Xiao Lei Zhu ◽

Hong Zhang ◽

Ming Hua Pu ◽

Yong Zhao

Keyword(s):

Energy Saving ◽

Chemical Solution Deposition ◽

Large Scale ◽

Coated Conductors ◽

Precursor Film ◽

Coated Conductor ◽

Chemical Solution ◽

Rapid Preparation ◽

Solution Deposition ◽

Decomposition Time

With Energy shortage becoming increasingly severe, the application of high-temperature superconducting material is considerable prospects. Economic, efficient and energy-saving preparation techniques become the key factors for the large-scale applications of REBa2Cu3O7-δ superconducting coated conductors. A rapid route of chemical solution deposition (CSD) has been developed to prepare YBa2Cu3O7-δ(YBCO) /SmBiO3 (SBO)/NiO/NiW coated conductor. In this process, the precursor film decomposition time is greatly reduced and the whole time of preparing SBO film is less than 2 hours. Therefore, it greatly saves the energy and reduces the cost of preparation. The subsequent YBCO layer was successfully grown on the SBO/NiO/NiW and the Tc is about 89 K and the Jc is about 1.46 MA/cm2 in self-field at 77 K. The results show that using rapid CSD preparation of YBCO/SBO/NiO/NiW is one of the effective energy-saving techniques.

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Fast and inexpensive whole-genome sequencing library preparation from intact yeast cells

G3 Genes|Genome|Genetics ◽

10.1093/g3journal/jkaa009 ◽

2020 ◽

Vol 11 (1) ◽

pp. 1-12

Author(s):

Sibylle C Vonesch ◽

Shengdi Li ◽

Chelsea Szu Tu ◽

Bianca P Hennig ◽

Nikolay Dobrev ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Genomic Dna ◽

Large Scale ◽

Massively Parallel Sequencing ◽

Yeast Cells ◽

Whole Genome ◽

High Quality ◽

Rapid Preparation ◽

Genomic Dna Isolation

Abstract Through the increase in the capacity of sequencing machines massively parallel sequencing of thousands of samples in a single run is now possible. With the improved throughput and resulting drop in the price of sequencing, the cost and time for preparation of sequencing libraries have become the major bottleneck in large-scale experiments. Methods using a hyperactive variant of the Tn5 transposase efficiently generate libraries starting from cDNA or genomic DNA in a few hours and are highly scalable. For genome sequencing, however, the time and effort spent on genomic DNA isolation limit the practicability of sequencing large numbers of samples. Here, we describe a highly scalable method for preparing high-quality whole-genome sequencing libraries directly from Saccharomyces cerevisiae cultures in less than 3 h at 34 cents per sample. We skip the rate-limiting step of genomic DNA extraction by directly tagmenting lysed yeast spheroplasts and add a nucleosome release step prior to enrichment PCR to improve the evenness of genomic coverage. Resulting libraries do not show any GC bias and are comparable in quality to libraries processed from genomic DNA with a commercially available Tn5-based kit. We use our protocol to investigate CRISPR/Cas9 on- and off-target edits and reliably detect edited variants and shared polymorphisms between strains. Our protocol enables rapid preparation of unbiased and high-quality, sequencing-ready indexed libraries for hundreds of yeast strains in a single day at a low price. By adjusting individual steps of our workflow, we expect that our protocol can be adapted to other organisms.

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Chitin of Araneae origin: structural features and biomimetic applications: a review

Applied Physics A ◽

10.1007/s00339-020-03867-x ◽

2020 ◽

Vol 126 (9) ◽

Cited By ~ 1

Author(s):

Tomasz Machałowski ◽

Chris Amemiya ◽

Teofil Jesionowski

Keyword(s):

Large Scale ◽

Environmental Science ◽

Structural Features ◽

Alternative Source ◽

Rapid Preparation ◽

Chemical Reagents ◽

Naturally Occurring ◽

Ready Availability ◽

Processing Wastes ◽

Seafood Processing

Abstract Large scale isolation of chitin traditionally has been carried out from fungal biomass as well as from seafood processing wastes, e.g., from shrimp, crab and lobster exoskeletons. Despite the relative abundance and ready availability of these materials, isolation of chitin requires great deal of chemical reagents and is time consuming. Obtained in this way chitin is produced in the form of powders, whiskers, and flakes. In this review, we have focused on the moulting cuticles of spiders as an alternative source of naturally occurring chitin. The comparatively high chitin content in the moults allows for rapid preparation of structures that maintain their original shape and integrity, such as the chitinous tubes from leg exoskeletons. Based on our latest scientific analyses regarding spider chitin, we highlight here its advantages and its biomimetic applications in tissue engineering, catalysis and environmental science.

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Formulation and Evaluation Pharmaceutical Aqueous Gel of Powdered Guava Leaves for Mouth Ulcer Treatment

Pharmatutor ◽

10.29161/pt.v6.i4.2018.32 ◽

2018 ◽

Vol 6 (4) ◽

pp. 32 ◽

Cited By ~ 1

Author(s):

S. Shaikh ◽

A. Shete ◽

R. Doijad

Keyword(s):

Mouth Ulcer ◽

Guava Leaves ◽

Ulcer Treatment

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One-Step Fabrication of a Functionally Integrated Device Based on Polydimethylsiloxane-Coated SiO2 NPs for Efficient and Continuous Oil Absorption

Materials ◽

10.3390/ma14205998 ◽

2021 ◽

Vol 14 (20) ◽

pp. 5998

Author(s):

Guannan Ju ◽

Lei Zhou ◽

Chang Jiao ◽

Jiafeng Shen ◽

Yihao Luan ◽

...

Keyword(s):

Rational Design ◽

Large Scale ◽

Rapid Preparation ◽

Hydrophobic Coating ◽

Novel Structure ◽

Engineering Strategy ◽

One Step ◽

Integrated Device ◽

Structure Engineering ◽

Spraying Method

The construction of superhydrophobic surfaces necessitates the rational design of topographic surface structure and the reduction of surface energy. To date, the reported strategies are usually complex with multi-steps and costly. Thus, the simultaneous achievement of the two indispensable factors is highly desired, yet rather challenging. Herein, we develop a novel structure engineering strategy of realizing the fabrication of a functionally integrated device (FID) with a superhydrophobic surface via a one-step spraying method. Specifically, silica nanoparticles are used to control the surface roughness of the device, while polydimethylsiloxane is employed as the hydrophobic coating. Benefitting from the adopted superhydrophobicity, the as-fabricated FID exhibits a continuous, excellent oil-water separating performance (e.g., 92.5% separating efficiency) when coupled with a peristaltic pump. Notably, a smart design of incorporating a gas switch is adopted in this device, thereby effectively preventing water from entering the FID, realizing thorough oil collection, and avoiding secondary pollution. This work opens up an avenue for the design and development of the FID, accessible for rapid preparation and large-scale practical application.

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