Kinetics and Thermodynamics of DNA, RNA, and Hybrid Duplex Formation

Biochemistry ◽  
2013 ◽  
Vol 52 (5) ◽  
pp. 765-772 ◽  
Author(s):  
Brittany Rauzan ◽  
Elizabeth McMichael ◽  
Rachel Cave ◽  
Lesley R. Sevcik ◽  
Kara Ostrosky ◽  
...  
Keyword(s):  
Kinetics And Thermodynamics ◽  
Hybrid Duplex ◽  
Duplex Formation

scholarly journals A Suite of Therapeutically-Inspired Nucleic Acid Logic Systems for Conditional Generation of Single-Stranded and Double-Stranded Oligonucleotides

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 615 ◽  
Author(s):  
Paul Zakrevsky ◽  
Eckart Bindewald ◽  
Hadley Humbertson ◽  
Mathias Viard ◽  
Nomongo Dorjsuren ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Boolean Logic ◽  
Hybrid Duplex ◽  
Therapeutic Development ◽  
Conditional Function ◽  
Regulatory Functions ◽  
Duplex Formation ◽  
Rna Biomarkers ◽  
Low Expression

Several varieties of small nucleic acid constructs are able to modulate gene expression via one of a number of different pathways and mechanisms. These constructs can be synthesized, assembled and delivered to cells where they are able to impart regulatory functions, presenting a potential avenue for the development of nucleic acid-based therapeutics. However, distinguishing aberrant cells in need of therapeutic treatment and limiting the activity of deliverable nucleic acid constructs to these specific cells remains a challenge. Here, we designed and characterized a collection of nucleic acids systems able to generate and/or release sequence-specific oligonucleotide constructs in a conditional manner based on the presence or absence of specific RNA trigger molecules. The conditional function of these systems utilizes the implementation of AND and NOT Boolean logic elements, which could ultimately be used to restrict the release of functionally relevant nucleic acid constructs to specific cellular environments defined by the high or low expression of particular RNA biomarkers. Each system is generalizable and designed with future therapeutic development in mind. Every construct assembles through nuclease-resistant RNA/DNA hybrid duplex formation, removing the need for additional 2′-modifications, while none contain any sequence restrictions on what can define the diagnostic trigger sequence or the functional oligonucleotide output.

Adsorption of safranine T from aqueous medium by nano mesoporous MCM-41: adsorption equilibrium, kinetics, and thermodynamics

2020 ◽  
Vol 10 ◽  
Author(s):  
Xiao-Dong Li ◽  
Qing-Zhou Zhai
Keyword(s):  
Electron Microscopy ◽  
Ph Value ◽  
Particle Diameter ◽  
Spherical Particles ◽  
Practical Significance ◽  
Average Particle ◽  
Average Pore ◽  
Kinetics And Thermodynamics ◽  
Safranine T ◽  
Mcm 41

Introduction: In industrial production, a small amount of saffron T emissions will cause increase of water color and increase of chemical oxygen consumption, so study of the decolorization of saffron T wastewater has an important practical significance. Methods: MCM (Mobil Composition of Matter)-41 molecular sieve was synthesized by hydrothermal method. Power Xray diffraction and scanning electron microscopy were used to characterize the sample. Safranine T dye was adsorbed from water by the MCM-41 prepared. Kinetics and thermodynamics of the adsorption were studied. Results: The MCM-41 sample presented spherical particles and regular. The BET (Brunner-Emmett-Teller) specific surface area of the sample determined by 77 K low temperature nitrogen adsorption-desorption isotherm was 932 m2 /g. Its average particle diameter was 110 nm. TEM (transmission electron microscopy) results showed that the sample structure presented a honeycomb pore structure and the average pore diameter was 3.0 nm. The results showed that when room temperature was 20 ± 1 ℃, adsorbate safranine T: adsorbent MCM-41 = 20 : 1，the optimum pH value of adsorption was 4.0 and contact time was 20 min, the adsorption rate reached 98.29% and the adsorption capacity was 19.66 mg/g. The entropy change and enthalpy change of the adsorption system are respectively ΔS0 = 157.5 J/(mol·K); ΔH0 = 21.544 kJ/mol. When temperature was 277.15, 293.15, 303.15 K，the free energy change was respectively △G1 0 = -22.107 kJ/mol, △G2 0 = -24.627 kJ/mol, △G3 0 = -26.202 kJ/mol. Conclusion: The adsorption of safranine T by MCM-41 belongs to a pseudo-second-order adsorption. This adsorption accords with the Freundlich equation and belongs to a heterogeneous adsorption. The adsorption is an endothermic reaction of entropy increase, being spontaneous.

Spectral property and application of self-duplex formation of an APy-substituted oligodeoxyadenylate

2008 ◽  
Author(s):  
Jeong Wu Yi ◽  
Young Jun Seo ◽  
Byeang Hyean Kim
Keyword(s):  
Spectral Property ◽  
Duplex Formation

scholarly journals Characterizing Binding Kinetics and Thermodynamics of Computer-Designed Nanobodies Targeting SARS-CoV-2 RBD

2021 ◽  
Vol 120 (3) ◽  
pp. 21a-22a
Author(s):  
Matheus Ferraz ◽  
Roberto Lins
Keyword(s):  
Binding Kinetics ◽  
Kinetics And Thermodynamics

scholarly journals Exploiting kinetics and thermodynamics to grow phase-pure complex oxides by molecular-beam epitaxy under continuous codeposition

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Eva H. Smith ◽  
Jon F. Ihlefeld ◽  
Colin A. Heikes ◽  
Hanjong Paik ◽  
Yuefeng Nie ◽  
...  
Keyword(s):  
Molecular Beam Epitaxy ◽  
Molecular Beam ◽  
Complex Oxides ◽  
Kinetics And Thermodynamics ◽  
Phase Pure ◽  
Pure Complex

scholarly journals Kinetics and thermodynamics of the formation of mixed micelles of egg phosphatidylcholine and bile salts.

1984 ◽  
Vol 25 (2) ◽  
pp. 135-147 ◽  
Author(s):  
N Rajagopalan ◽  
S Lindenbaum
Keyword(s):  
Bile Salts ◽  
Mixed Micelles ◽  
Kinetics And Thermodynamics
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