Quantitative Hybridization Kinetics of DNA Probes to RNA in Solution Followed by Diffusional Fluorescence Correlation Analysis†

Biochemistry ◽  
1996 ◽  
Vol 35 (31) ◽  
pp. 10182-10193 ◽  
Author(s):  
Petra Schwille ◽  
Frank Oehlenschläger ◽  
Nils G. Walter
Keyword(s):  
Correlation Analysis ◽  
Dna Probes ◽  
Fluorescence Correlation ◽  
Kinetics Of

Interaction Kinetics of Tetramethylrhodamine Transferrin with Human Transferrin Receptor Studied by Fluorescence Correlation Spectroscopy

Biochemistry ◽  
1999 ◽  
Vol 38 (26) ◽  
pp. 8402-8408 ◽  
Author(s):  
Jens Schüler ◽  
Joachim Frank ◽  
Ulrike Trier ◽  
Monika Schäfer-Korting ◽  
Wolfram Saenger
Keyword(s):  
Transferrin Receptor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Human Transferrin ◽  
Human Transferrin Receptor ◽  
Interaction Kinetics ◽  
Kinetics Of

Hybridization kinetics of double-stranded DNA probes for rapid molecular analysis

The Analyst ◽  
2009 ◽  
Vol 134 (8) ◽  
pp. 1675 ◽  
Author(s):  
Vinay Gidwani ◽  
Reza Riahi ◽  
Donna D. Zhang ◽  
Pak Kin Wong
Keyword(s):  
Molecular Analysis ◽  
Dna Probes ◽  
Double Stranded Dna ◽  
Kinetics Of

Correlation analysis of reactivity in the addition of substituted benzylamines to β-nitrostyrene

1996 ◽  
Vol 74 (4) ◽  
pp. 625-629 ◽  
Author(s):  
Neeta Jalani ◽  
Seema Kothari ◽  
Kalyan K. Banerji
Keyword(s):  
Activation Energy ◽  
Correlation Analysis ◽  
Rate Constant ◽  
Steric Hindrance ◽  
Nucleophilic Addition ◽  
Regression Coefficients ◽  
Nucleophilic Attack ◽  
Arrhenius Activation Energy ◽  
First Order ◽  
Kinetics Of

The kinetics of addition of a number of ortho-, meta-, and para-substituted benzylamines to β-nitrostyrene (NS) in acetonitrile have been studied. The reaction is first order with respect to NS. The order with respect to the amine is higher than one. It has been shown that the reaction follows two mechanistic pathways, uncatalyzed and catalyzed by the amine. The Arrhenius activation energy for the catalyzed path is negative, indicating the presence of a pre-equilibrium (k1, k−1) leading to the formation of a zwitterion. The values of the rate constant, k1, for the nucleophilic attack have been determined for 28 benzylamines. The rate constant k1 was subjected to correlation analysis using Charton's LDR and LDRS equations. The polar regression coefficients are negative, indicating the formation of a cationic species in the transition state. The reaction is subject to steric hindrance by ortho substituents. Key words: nucleophilic addition, benzylamines, correlation analysis, kinetics, alkene.

scholarly journals Unveiling the multi-step solubilization mechanism of single vesicles by detergents

2019 ◽  
Author(s):  
Paul A. Dalgarno ◽  
José Juan-Colás ◽  
Gordon J. Hedley ◽  
Lucas Piñeiro ◽  
Mercedes Novo ◽  
...  
Keyword(s):  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Correlation Spectroscopy ◽  
Optical Methods ◽  
Fluorescence Correlation ◽  
Ionic Detergent ◽  
Triton X ◽  
Kinetics Of ◽  
Single Vesicle

AbstractThe solubilization of membranes by detergents is critical for many technological applications and has become widely used in biochemistry research to induce cell rupture, extract cell constituents, and to purify, reconstitute and crystallize membrane proteins. The thermodynamic details of solubilization have been extensively investigated, but the kinetic aspects remain poorly understood. Here we used a combination of single-vesicle Förster resonance energy transfer (svFRET), fluorescence correlation spectroscopy and quartz-crystal microbalance with dissipation monitoring to access the real-time kinetics and elementary solubilization steps of sub-micron sized vesicles, which are inaccessible by conventional diffraction-limited optical methods. Real-time injection of a non-ionic detergent, Triton X, induced biphasic solubilization kinetics of surface-immobilized vesicles labelled with the Dil/DiD FRET pair. The nanoscale sensitivity accessible by svFRET allowed us to unambiguously assign each kinetic step to distortions of the vesicle structure comprising an initial fast vesicle-swelling event followed by slow lipid loss and micellization. We expect the svFRET platform to be applicable beyond the sub-micron sizes studied here and become a unique tool to unravel the complex kinetics of detergent-lipid interactions.

Molecular Exchange Kinetics of Diblock Copolymer Micelles Monitored by Fluorescence Correlation Spectroscopy

2014 ◽  
Vol 3 (5) ◽  
pp. 428-432 ◽  
Author(s):  
David Schaeffel ◽  
Andreas Kreyes ◽  
Yi Zhao ◽  
Katharina Landfester ◽  
Hans-Jürgen Butt ◽  
...  
Keyword(s):  
Diblock Copolymer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Exchange Kinetics ◽  
Copolymer Micelles ◽  
Kinetics Of

Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin α-bungarotoxin with Torpedo californica acetylcholine receptor

1996 ◽  
Vol 58 (1-2) ◽  
pp. 3-12 ◽  
Author(s):  
Barbara Rauer ◽  
Eberhard Neumann ◽  
Jerker Widengren ◽  
Rudolf Rigler
Keyword(s):  
Acetylcholine Receptor ◽  
Torpedo Californica ◽  
Fluorescence Correlation ◽  
Interaction Kinetics ◽  
Kinetics Of

Kinetics of Insulin Adsorption at the Oil–Water Interface and Diffusion Properties of Adsorbed Layers Monitored Using Fluorescence Correlation Spectroscopy

2006 ◽  
Vol 23 (1) ◽  
pp. 148-155 ◽  
Author(s):  
Jesper Donsmark ◽  
Lene Jorgensen ◽  
Susanne Mollmann ◽  
Sven Frokjaer ◽  
Christian Rischel
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Water Interface ◽  
Fluorescence Correlation ◽  
Adsorbed Layers ◽  
Oil Water ◽  
Kinetics Of ◽  
And Diffusion ◽  
Diffusion Properties

Hybridization Reaction Kinetics of DNA Probes on Beads Arrayed in a Capillary Enhanced by Turbulent Flow

2003 ◽  
Vol 75 (13) ◽  
pp. 3079-3085 ◽  
Author(s):  
Yoshinobu Kohara
Keyword(s):  
Turbulent Flow ◽  
Reaction Kinetics ◽  
Dna Probes ◽  
Kinetics Of ◽  
Hybridization Reaction

scholarly journals Full Fluorescence Correlation Analysis - From Picoseconds To Seconds- For The Study Of Biomolecular Interactions And Dynamics

2009 ◽  
Vol 96 (3) ◽  
pp. 26a
Author(s):  
Samantha Fore ◽  
Felix Koberling ◽  
Michael Wahl ◽  
Thomas Huser ◽  
Sonny Ly ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Biomolecular Interactions ◽  
Fluorescence Correlation
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