Kinetics of Insulin Adsorption at the Oil–Water Interface and Diffusion Properties of Adsorbed Layers Monitored Using Fluorescence Correlation Spectroscopy

2006 ◽  
Vol 23 (1) ◽  
pp. 148-155 ◽  
Author(s):  
Jesper Donsmark ◽  
Lene Jorgensen ◽  
Susanne Mollmann ◽  
Sven Frokjaer ◽  
Christian Rischel
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Water Interface ◽  
Fluorescence Correlation ◽  
Adsorbed Layers ◽  
Oil Water ◽  
Kinetics Of ◽  
And Diffusion ◽  
Diffusion Properties

Molecular Exchange Kinetics of Diblock Copolymer Micelles Monitored by Fluorescence Correlation Spectroscopy

2014 ◽  
Vol 3 (5) ◽  
pp. 428-432 ◽  
Author(s):  
David Schaeffel ◽  
Andreas Kreyes ◽  
Yi Zhao ◽  
Katharina Landfester ◽  
Hans-Jürgen Butt ◽  
...  
Keyword(s):  
Diblock Copolymer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Exchange Kinetics ◽  
Copolymer Micelles ◽  
Kinetics Of

scholarly journals Fluorescence Correlation Spectroscopy To Study Diffusion and Reaction of Bacteriophages inside Biofilms

2008 ◽  
Vol 74 (7) ◽  
pp. 2135-2143 ◽  
Author(s):  
R. Briandet ◽  
P. Lacroix-Gueu ◽  
M. Renault ◽  
S. Lecart ◽  
T. Meylheuc ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Extracellular Polymeric Substances ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Two Photon ◽  
Photon Excitation ◽  
Viral Particles ◽  
Resistant Strains ◽  
And Diffusion

ABSTRACT In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococcal c2 phage diffusion and reaction through model biofilms using in situ fluorescence correlation spectroscopy with two-photon excitation. Correlation curves for fluorescently labeled c2 phage in nonreacting Stenotrophomonas maltophilia biofilms indicated that extracellular polymeric substances did not provide significant resistance to phage penetration and diffusion, even though penetration and diffusion were sometimes restricted because of the noncontractile tail of the viral particle. Fluctuations in the fluorescence intensity of the labeled phage were detected throughout the thickness of biofilms formed by c2-sensitive and c2-resistant strains of Lactococcus lactis but could never be correlated with time, revealing that the phage was immobile. This finding confirmed that recognition binding receptors for the viral particles were present on the resistant bacterial cell wall. Taken together, our results suggest that biofilms may act as “active” phage reservoirs that can entrap and amplify viral particles and protect them from harsh environments.

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