scholarly journals Phylogenetic Microarray Analysis of a Microbial Community Performing Reductive Dechlorination at a TCE-Contaminated Site

2011 ◽  
Vol 46 (2) ◽  
pp. 1044-1054 ◽  
Author(s):  
Patrick K. H. Lee ◽  
F. Warnecke ◽  
Eoin L. Brodie ◽  
Tamzen W. Macbeth ◽  
Mark E. Conrad ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microarray Analysis ◽  
Reductive Dechlorination ◽  
Contaminated Site

scholarly journals Evaluating the aerobic xylene-degrading potential of the intrinsic microbial community of a legacy BTEX-contaminated aquifer by enrichment culturing coupled with multi-omics analysis: uncovering the role of Hydrogenophaga strains in xylene degradation

2022 ◽  
Author(s):  
Sinchan Banerjee ◽  
Anna Bedics ◽  
Péter Harkai ◽  
Balázs Kriszt ◽  
Nagaraju Alpula ◽  
...  
Keyword(s):  
Microbial Community ◽  
Primary Objective ◽  
Community Diversity ◽  
Contaminated Site ◽  
Whole Genome Analysis ◽  
Contaminated Aquifer ◽  
Degrading Bacteria ◽  
Research Outcome ◽  
Xylene Isomers ◽  
Set Up

AbstractTo develop effective bioremediation strategies, it is always important to explore autochthonous microbial community diversity using substrate-specific enrichment. The primary objective of this present study was to reveal the diversity of aerobic xylene-degrading bacteria at a legacy BTEX-contaminated site where xylene is the predominant contaminant, as well as to identify potential indigenous strains that could effectively degrade xylenes, in order to better understand the underlying facts about xylene degradation using a multi-omics approach. Henceforward, parallel aerobic microcosms were set up using different xylene isomers as the sole carbon source to investigate evolved bacterial communities using both culture-dependent and independent methods. Research outcome showed that the autochthonous community of this legacy BTEX-contaminated site has the capability to remove all of the xylene isomers from the environment aerobically employing different bacterial groups for different xylene isomers. Interestingly, polyphasic analysis of the enrichments disclose that the community composition of the o-xylene-degrading enrichment community was utterly distinct from that of the m- and p-xylene-degrading enrichments. Although in each of the enrichments Pseudomonas and Acidovorax were the dominant genera, in the case of o-xylene-degrading enrichment Rhodococcus was the main player. Among the isolates, two Hydogenophaga strains, belonging to the same genomic species, were obtained from p-xylene-degrading enrichment, substantially able to degrade aromatic hydrocarbons including xylene isomers aerobically. Comparative whole-genome analysis of the strains revealed different genomic adaptations to aromatic hydrocarbon degradation, providing an explanation on their different xylene isomer-degrading abilities.

Monitoring Microbial Community Structure and Dynamics duringin situU(VI) Bioremediation with a Field-Portable Microarray Analysis System

2010 ◽  
Vol 44 (14) ◽  
pp. 5516-5522 ◽  
Author(s):  
Darrell P. Chandler ◽  
Alexander Kukhtin ◽  
Rebecca Mokhiber ◽  
Christopher Knickerbocker ◽  
Dora Ogles ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microarray Analysis ◽  
Microbial Community Structure ◽  
Structure And Dynamics ◽  
Analysis System

scholarly journals Geochemical and microbial community determinants of reductive dechlorination at a site biostimulated with glycerol

2016 ◽  
Vol 19 (3) ◽  
pp. 968-981 ◽  
Author(s):  
Siavash Atashgahi ◽  
Yue Lu ◽  
Ying Zheng ◽  
Edoardo Saccenti ◽  
Maria Suarez-Diez ◽  
...  
Keyword(s):  
Microbial Community ◽  
Reductive Dechlorination ◽  
A Site

Natural attenuation of chloroethenes: identification of sequential reductive/oxidative biodegradation by microcosm studies

2008 ◽  
Vol 58 (5) ◽  
pp. 1137-1145 ◽  
Author(s):  
K. R. Schmidt ◽  
A. Tiehm
Keyword(s):  
Reductive Dechlorination ◽  
Anaerobic Degradation ◽  
Reduction Potential ◽  
Oxidative Degradation ◽  
Contaminated Site ◽  
Low Oxygen ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Reductive Degradation ◽  
Microcosm Studies

A different lines of evidence approach for investigation of biodegradation processes at a chloroethene contaminated site showed well corresponding results of pollutant profiles, redox zonation, characterisation of autochthonic microflora and microcosm studies. In particular microcosm studies allowed identification of the predominating degradation pathways. Perchloroethene and trichloroethene are reductively chlorinated to mainly cis-1,2-dichloroethene (cDCE) under anaerobic conditions. Further reductive degradation to vinyl chloride (VC) is restricted to a distinct strongly anaerobic zone in the plume. Addition of high amounts of sediment material (80 vol%) to groundwater microcosms enabled reductive dechlorination without amendment with further auxiliary substrates. Reductive dechlorination was not irreversibly hindered by initially high nitrate concentrations and initially high oxidation–reduction potential. The products of anaerobic degradation cDCE and VC are subsequently aerobically mineralised, even when only low oxygen concentrations are available. Anaerobic oxidative degradation could not be proven in this study.

Environmental proteomics reveals early microbial community responses to biostimulation at a uranium- and nitrate-contaminated site

PROTEOMICS ◽  
2013 ◽  
Vol 13 (18-19) ◽  
pp. 2921-2930 ◽  
Author(s):  
Karuna Chourey ◽  
Silke Nissen ◽  
Tatiana Vishnivetskaya ◽  
Manesh Shah ◽  
Susan Pfiffner ◽  
...  
Keyword(s):  
Microbial Community ◽  
Contaminated Site ◽  
Community Responses

scholarly journals Hydrogeochemical and hydrodynamic features affecting redox processes in groundwater

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Diego Di Curzio
Keyword(s):  
Reductive Dechlorination ◽  
Chlorinated Solvents ◽  
Organic Substrate ◽  
Electron Acceptors ◽  
Contaminated Site ◽  
Hydrodynamic Effect ◽  
Redox Processes ◽  
Dispersive Transport ◽  
Factorial Kriging

This research deals with some hydrogeochemical and hydrodynamic features that affect redox processes in groundwater, presenting three different case studies and methodological approaches. This information is vital when characterizing contaminated sites, planning monitoring, selecting remedial techniques, and assessing health risks. In the San Pedro Sula site case study (Honduras), new insights and an advanced method for the aquifer redox zonation were provided. The Multi-Collocated Factorial Kriging, a multivariate geostatistical technique borrowed from agriculture and soil science, identified simultaneous redox processes, acting at different scales and mainly due to eutrophicated surface-water/groundwater interaction: at short-range, Mn oxihydroxide reductive dissolution and Fe oxi-hydroxide colloidal phase precipitation; at long-range, Fe mobilization. The obtained results are supported by the Principal Component Analysis and hydrogeochemical numerical modeling. The interaction among different contamination events in the VR site allowed to get a deeper insight into the competition for organic substrate among different redox processes. To this purpose, a 1-D Advective-Reactive- Dispersive transport numerical model was implemented, and its results highlighted that the reductive dechlorination is inhibited by the inorganic Terminal Electron Accepting Processes, enriching groundwater in highly toxic by-products of the reductive dechlorination (i.e. Vinyl Chloride). As a matter of fact, the bacteria using inorganic Terminal Electron Acceptors to oxidize the organic substrate are more efficient than the halo-respiring bacteria, that favor reductive dechlorination. The contaminated site of Bussi Officine case study allowed to clarify the hydrodynamic effect on the redox processes and the anoxic condition distribution. The comparison between dissolved chlorinated solvents and the inorganic Terminal Electron Acceptors distribution in the aquifer pointed out that dispersion inhibits all the redox processes because it dilutes the organic substrate concentration in groundwater. On the other hand, the lacustrine deposits rich organic matter (i.e. peat) can be considered as “chemical reactors”, that releases by back-diffusion toxic by-product of the reductive dichlorination in groundwater.

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