Enzyme-linked immunosorbent assay for quantitation of neomycin phosphotransferase II in genetically modified cotton tissue extracts

1992 ◽  
Vol 40 (8) ◽  
pp. 1453-1458 ◽  
Author(s):  
Glen J. Rogan ◽  
Joel E. Ream ◽  
Sharon A. Berberich ◽  
Roy L. Fuchs
Keyword(s):  
Immunosorbent Assay ◽  
Genetically Modified ◽  
Enzyme Linked Immunosorbent Assay ◽  
Neomycin Phosphotransferase ◽  
Neomycin Phosphotransferase Ii ◽  
Genetically Modified Cotton ◽  
Tissue Extracts

Sensitive enzyme-linked immunosorbent assay for detection of PrPSc in crude tissue extracts from scrapie-affected mice

1997 ◽  
Vol 64 (2) ◽  
pp. 205-216 ◽  
Author(s):  
Kai-Uwe D. Grathwohl ◽  
Motohiro Horiuchia ◽  
Naotaka Ishiguro ◽  
Morikazu Shinagawa
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Extracts

Enzyme linked immunosorbent assay for PAT protein detection in genetically modified rape

2006 ◽  
Vol 3 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Xu Wen-Tao ◽  
Huang Kun-Lun ◽  
Deng Ai-Ke ◽  
Luo Yun-Bo
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Genetically Modified ◽  
Protein A ◽  
Protein Detection ◽  
Cross Reactivity ◽  
Enzymic Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sepharose 4B ◽  
Pat Protein

AbstractWe have developed and applied an immunoassay method to detect genetically modified (GM) rape containing phosphinothricin acetyltransferase (PAT). The purified PAT was identified by Western blotting and enzymic activity analysis. The polyclonal antibody against purified PAT protein was obtained and purified by both a saturated ammonium sulphate method and protein A-Sepharose 4B. The sensitivity and cross-reactivity of the polyclonal antibody has been demonstrated in an enzyme-linked immunosorbent assay (ELISA). The result of the ELISA for antiserum sensitivity was about 2×10−5mg/ml and the cross-reactivity determined experimentally showed a high degree of specificity for the antiserum used, because values were all less than 0.1%. Detection of transgenic plants was evaluated using two transgenic rape lines (MS1/RF1 and MS8/RF3) which could be easily distinguished by ELISA.

Purification and characterization of neomycin phosphotransferase II from genetically modified cottonseed (Gossypium hirsutum)

1995 ◽  
Vol 43 (4) ◽  
pp. 1105-1109 ◽  
Author(s):  
David C. Wood ◽  
Linh V. Vu ◽  
Nancee M. Kimack ◽  
Glennon J. Rogan ◽  
Joel E. Ream ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genetically Modified ◽  
Neomycin Phosphotransferase ◽  
Purification And Characterization ◽  
Neomycin Phosphotransferase Ii

Urokinase-type receptor in breast cancer tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- and polyclonal antibodies

1994 ◽  
Vol 8 ◽  
pp. 123 ◽  
Author(s):  
J. Grøndahl-Hansen ◽  
N. Brünner ◽  
G.M. Clark ◽  
K. Danø ◽  
G. Høyer-Hansen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Breast Cancer Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts ◽  
Type Receptor

Quantitative Assessment of HER-2/Neu Protein Concentration in Breast Cancer by Enzyme-Linked Immunosorbent Assay

2003 ◽  
Vol 18 (1) ◽  
pp. 13-20 ◽  
Author(s):  
V. Müller ◽  
C. Thomssen ◽  
C. Karakas ◽  
I. Eustermann ◽  
J. Ramirez Porras ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Amplification ◽  
Immunosorbent Assay ◽  
Immunohistochemical Staining ◽  
Protein Concentration ◽  
Tumor Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts ◽  
Her 2

Purpose The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). Experimental design Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). Results Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p<0.0001, Mann-Whitney U test). A significant correlation between p185 concentration and immunohistochemical staining was observed (p<0.0001, Kruskal-Wallis test). In addition, p185 concentration measured by ELISA was correlated with the degree of HER-2/neu gene amplification determined by CISH. HER-2/neu-amplified tumors had significantly higher p185 concentrations (median value 181 ng/mg protein) than non-amplified tumors (median value 20 ng/mg; p<0.0001, Mann-Whitney U test). Conclusions ELISA-based measurement of HER-2/neu protein concentration in breast cancer tissue extracts is feasible and provides quantitative results for p185 protein concentrations that correlate closely with HER-2/neu immunoscore and gene amplification.

scholarly journals Alergenisitas Sistem Glikasi Isolat Protein Kedelai-Fruktooligosakarida (Allergenicity Properties of Soy Protein Isolate-Fructooligosaccaride Glycation Systems)

2017 ◽  
Vol 36 (4) ◽  
pp. 450 ◽  
Author(s):  
Rahayu Suseno ◽  
Nurheni Sri Palupi ◽  
Endang Prangdimurti
Keyword(s):  
Immunosorbent Assay ◽  
Soy Protein ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Soy Protein Isolate ◽  
Immunological Response ◽  
Liquid System ◽  
Protein Isolate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Genetically Modified Food

Food allergy is an immunological response caused by allergens contained in food. Soybean is one of the eight kinds of food products that can cause allergies. Genetically modified food crops that are most widely produced worldwide is soybean (47 % worldwide). Genetically Modified Organisms (GMO) products is concerned may increase the allergenicity of the  product. The aims of the research were to study the allergenicity of GMO and non-GMO Soy Protein Isolates (SPI) and the glycation effect to allergenicity of SPI. GMO and non-GMO SPI were glycated with fructooligosaccharides (FOS) through the Maillard reaction in liquid systems. Allergenicity was determined qualitatively using immunoblotting and quantitatively using Enzyme-Linked Immunosorbent Assay (ELISA). The glycation degree of GMO and non-GMO SPI can increase up to 75.03 % and 73.50 % in the liquid system. There were 9 protein allergens in GMO soybean and 8 protein allergens in non-GMO soybean. The glycation reaction could reduce protein allergens in GMO and non-GMO SPI up to 91.69 % and 87.07 %.ABSTRAKAlergi pangan merupakan sebuah respon imunologis yang disebabkan oleh alergen yang terdapat pada pangan. Kacang kedelai merupakan satu dari delapan jenis bahan pangan yang sering menyebabkan alergi. Tanaman pangan hasil rekayasa genetika (GMO) yang banyak diproduksi di dunia adalah kacang kedelai yaitu sekitar 47 %. Produk GMO dikhawatirkan dapat meningkatkan alergenisitasnya. Penelitian ini bertujuan untuk mempelajari tinggat alergenisitas antara Isolat Protein Kedelai (IPK) GMO dan non-GMO serta pengaruh glikasi terhadap alergenisitas IPK. IPK GMO dan non-GMO diglikasi dengan fruktooligosakarida melalui reaksi Maillard dengan sistem cair. Alergenisitas diukur secara kualitatif menggunakan immunobloting dan secara kuantitatif menggunakan Enzyme-Linked Immunosorbent Assay (ELISA). Peningkatan derajat glikasi IPK GMO dan non-GMO pada sistem cair masing-masing memperlihatkan hasil 75,03 % dan 73,50 %. Terdapat 9 protein alergen pada kacang kedelai GMO dan 8 protein alergen pada kacang kedelai non-GMO. Reaksi glikasi dapat mengurangi alergen pada kacang kedelai GMO dan non-GMO hingga 91,69% dan 87,07%.

Urokinase receptor in breast cancer tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- and polyclonal antibodies

1995 ◽  
Vol 33 (3) ◽  
pp. 199-207 ◽  
Author(s):  
E. R�nne ◽  
G. H�yer-Hansen ◽  
N. Br�nner ◽  
H. Pedersen ◽  
F. Rank ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Breast Cancer Tissue ◽  
Urokinase Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts
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