scholarly journals Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

2002 ◽  
Vol 50 (2) ◽  
pp. 248-254 ◽  
Author(s):  
John M. DeLong ◽  
Robert K. Prange ◽  
D. Mark Hodges ◽  
Charles F. Forney ◽  
M. Conny Bishop ◽  
...  

1996 ◽  
Vol 79 (4) ◽  
pp. 995-997 ◽  
Author(s):  
Kutlay M Burat ◽  
Onur Bozkurt

Abstract The linearity range of the standard Fe+3 calibration curve for measuring lipid hydroperoxides in food samples by the modified ferrous oxidation-xylenol orange (mFOX) method was extended from 5-20 μg to 2-40 μg by establishing the best concentration of xylenol orange in the same assay system. Butter, fish oil, and some vegetable oils were analyzed by the International Dairy Federation method and the improved mFOX method, and results were compared.



Author(s):  
V. V. Skarga ◽  
E. V. Nevezhin ◽  
A. А. Matrosov ◽  
V. V. Negrebetsky ◽  
M. V. Malakhov

Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen.



1998 ◽  
Vol 37 (3) ◽  
pp. 137-146 ◽  
Author(s):  
Eva Södergren ◽  
Jaffar Nourooz-Zadeh ◽  
Lars Berglund ◽  
Bengt Vessby




2004 ◽  
Vol 106 (10) ◽  
pp. 688-696 ◽  
Author(s):  
Jos� A. Navas ◽  
Alba Tres ◽  
Rafael Codony ◽  
Josep Boatella ◽  
Ricard Bou ◽  
...  


2000 ◽  
Vol 48 (9) ◽  
pp. 4136-4143 ◽  
Author(s):  
Anna Grau ◽  
Rafael Codony ◽  
Magda Rafecas ◽  
Ana C. Barroeta ◽  
Francesc Guardiola


2020 ◽  
Vol 97 (5) ◽  
pp. 509-516
Author(s):  
Reem Abuzaytoun ◽  
Suzanne Budge ◽  
Lisbeth Truelstrup Hansen ◽  
Shawna MacKinnon


1995 ◽  
Vol 43 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Jaffar Nourooz-Zadeh ◽  
Javad Tajaddini-Sarmadi ◽  
Simon P. Wolff




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