Measurement of Hydroperoxides in Edible Oils Using the Ferrous Oxidation in Xylenol Orange Assay

1995 ◽  
Vol 43 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Jaffar Nourooz-Zadeh ◽  
Javad Tajaddini-Sarmadi ◽  
Simon P. Wolff

2000 ◽  
Vol 48 (9) ◽  
pp. 4136-4143 ◽  
Author(s):  
Anna Grau ◽  
Rafael Codony ◽  
Magda Rafecas ◽  
Ana C. Barroeta ◽  
Francesc Guardiola


2020 ◽  
Vol 97 (5) ◽  
pp. 509-516
Author(s):  
Reem Abuzaytoun ◽  
Suzanne Budge ◽  
Lisbeth Truelstrup Hansen ◽  
Shawna MacKinnon


1996 ◽  
Vol 79 (4) ◽  
pp. 995-997 ◽  
Author(s):  
Kutlay M Burat ◽  
Onur Bozkurt

Abstract The linearity range of the standard Fe+3 calibration curve for measuring lipid hydroperoxides in food samples by the modified ferrous oxidation-xylenol orange (mFOX) method was extended from 5-20 μg to 2-40 μg by establishing the best concentration of xylenol orange in the same assay system. Butter, fish oil, and some vegetable oils were analyzed by the International Dairy Federation method and the improved mFOX method, and results were compared.



2002 ◽  
Vol 50 (2) ◽  
pp. 248-254 ◽  
Author(s):  
John M. DeLong ◽  
Robert K. Prange ◽  
D. Mark Hodges ◽  
Charles F. Forney ◽  
M. Conny Bishop ◽  
...  


Author(s):  
V. V. Skarga ◽  
E. V. Nevezhin ◽  
A. А. Matrosov ◽  
V. V. Negrebetsky ◽  
M. V. Malakhov

Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen.





2006 ◽  
Vol 351 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Young Sik Cho ◽  
Hyo Sun Kim ◽  
Chi Hyun Kim ◽  
Hyae Gyeong Cheon


2019 ◽  
Vol 7 (10) ◽  
pp. 347
Author(s):  
Aguilar Vitorino ◽  
Pastrana Alta ◽  
Ortega

Levels of lipid peroxidation in hepatopancreas (HP), gill (G), and hemolymph (HYM) of stone violaceous crab Platyxanthus orbignyi (Milne Edwards and Lucas (1843)) were performed to examine the effect of short exposure to air. After four hours animals were collected, 14 from exposure to air and 10 from seawater were dissected and their lipid peroxidation (LPO) levels were evaluated using the ferrous oxidation-xylenol orange (FOX) method, in gill, hepatopancreas, and hemolymph. The total mortality of those crabs was evaluated after seven hours at 22 ± 1 °C on exposure to air conditions. Levels of LPO in hepatopancreas (female/male = 4.68 ± 1.60/5.12 ± 1.59 Eq-H2O2/g wet tissue) and hemolymph (female/male = 1.48 ± 1.42/1.28 ± 1.06 Eq-H2O2/g wet tissue) displayed no significant differences, in contrast, gills displayed significant differences (male/female = 5.63 ± 0.83/4.63 ± 0.44 Eq-H2O2/g wet tissue, p < 0.05). The results showed that air exposure in the short term in this study induces a different response in oxidative stress levels and damage could be accompanied by accumulation of peroxide lipids (LOOH). These results suggest that different organs can show different responses to oxidative stress between male and female crabs to this species.



2009 ◽  
Vol 111 (6) ◽  
pp. 563-573 ◽  
Author(s):  
Alba Tres ◽  
Carla Daniela Nuchi ◽  
Ricard Bou ◽  
Rafael Codony ◽  
Francesc Guardiola


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