Lipid Hydroperoxide Determination in Dark Chicken Meat through a Ferrous Oxidation−Xylenol Orange Method

2000 ◽  
Vol 48 (9) ◽  
pp. 4136-4143 ◽  
Author(s):  
Anna Grau ◽  
Rafael Codony ◽  
Magda Rafecas ◽  
Ana C. Barroeta ◽  
Francesc Guardiola
Keyword(s):  
Xylenol Orange ◽  
Lipid Hydroperoxide ◽  
Chicken Meat ◽  
Ferrous Oxidation

Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method

Lipids ◽  
1991 ◽  
Vol 26 (10) ◽  
pp. 853-856 ◽  
Author(s):  
Zhen-Yue Jiang ◽  
Alison C. S. Woollard ◽  
Simon P. Wolff
Keyword(s):  
Xylenol Orange ◽  
Lipid Hydroperoxide

Modification of the Ferrous Oxidation‐Xylenol Orange Method for Determination of Peroxide Value in Highly Pigmented Sea Cucumber Viscera Lipid

2020 ◽  
Vol 97 (5) ◽  
pp. 509-516
Author(s):  
Reem Abuzaytoun ◽  
Suzanne Budge ◽  
Lisbeth Truelstrup Hansen ◽  
Shawna MacKinnon
Keyword(s):  
Peroxide Value ◽  
Sea Cucumber ◽  
Xylenol Orange ◽  
Ferrous Oxidation

scholarly journals Improvement of Calibration Curve for Determining Peroxide Values of Food Lipids by the Modified Ferrous Oxidation-Xylenol Orange Method

1996 ◽  
Vol 79 (4) ◽  
pp. 995-997 ◽  
Author(s):  
Kutlay M Burat ◽  
Onur Bozkurt
Keyword(s):  
Fish Oil ◽  
Vegetable Oils ◽  
Calibration Curve ◽  
Xylenol Orange ◽  
Food Samples ◽  
Assay System ◽  
Lipid Hydroperoxides ◽  
Peroxide Values ◽  
Ferrous Oxidation ◽  
International Dairy Federation

Abstract The linearity range of the standard Fe+3 calibration curve for measuring lipid hydroperoxides in food samples by the modified ferrous oxidation-xylenol orange (mFOX) method was extended from 5-20 μg to 2-40 μg by establishing the best concentration of xylenol orange in the same assay system. Butter, fish oil, and some vegetable oils were analyzed by the International Dairy Federation method and the improved mFOX method, and results were compared.

Measurement of Hydroperoxides in Edible Oils Using the Ferrous Oxidation in Xylenol Orange Assay

1995 ◽  
Vol 43 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Jaffar Nourooz-Zadeh ◽  
Javad Tajaddini-Sarmadi ◽  
Simon P. Wolff
Keyword(s):  
Edible Oils ◽  
Xylenol Orange ◽  
Ferrous Oxidation

scholarly journals Using a Modified Ferrous Oxidation−Xylenol Orange (FOX) Assay for Detection of Lipid Hydroperoxides in Plant Tissue

2002 ◽  
Vol 50 (2) ◽  
pp. 248-254 ◽  
Author(s):  
John M. DeLong ◽  
Robert K. Prange ◽  
D. Mark Hodges ◽  
Charles F. Forney ◽  
M. Conny Bishop ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Xylenol Orange ◽  
Lipid Hydroperoxides ◽  
Fox Assay ◽  
Ferrous Oxidation

scholarly journals DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN

2019 ◽  
Vol 14 (1) ◽  
pp. 32-38
Author(s):  
V. V. Skarga ◽  
E. V. Nevezhin ◽  
A. А. Matrosov ◽  
V. V. Negrebetsky ◽  
M. V. Malakhov
Keyword(s):  
Quantitative Analysis ◽  
Phosphate Buffer ◽  
Biological Effects ◽  
Xylenol Orange ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Uva Irradiation ◽  
Fox Assay ◽  
Kinetics Of ◽  
Ferrous Oxidation

Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen.

scholarly journals Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Relevance to determination of total plasma lipid hydroperoxide concentrations

1996 ◽  
Vol 313 (3) ◽  
pp. 781-786 ◽  
Author(s):  
Jaffar NOUROOZ-ZADEH ◽  
Jarad TAJADDINI-SARMADI ◽  
K. L. Eddie LING ◽  
Simon P. WOLFF
Keyword(s):  
Total Lipid ◽  
Xylenol Orange ◽  
Low Density Lipoprotein ◽  
Lipid Hydroperoxide ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Ldl Oxidation ◽  
Lipid Hydroperoxides ◽  
Very Low Density Lipoprotein ◽  
Low Density

High-density lipoprotein (HDL) has been proposed as the principal carrier of hydroperoxides in plasma, based upon data gathered with an HPLC-chemiluminescence technique. To test this hypothesis we have measured total lipid hydroperoxides in native plasma using the ferrous oxidation in Xylenol Orange (FOX) assay and then fractionated plasma into very-low-density lipoprotein, low-density lipoprotein (LDL) and HDL fractions. Hydroperoxides were found to accumulate principally (more than 65%) in LDL, as judged by hydroperoxide content per amount of protein or cholesterol, or expressed as a proportion of total hydroperoxide in plasma. Plasma was also incubated at 37 °C in the presence and absence of 2,2´-azo-bis-(2-amidinopropane) hydrochloride (AAPH), an azo-initiator of lipid peroxidation. The majority of hydroperoxides generated in plasma were recovered in the LDL fraction. Furthermore, when isolated lipoproteins were subject to oxidation initiated by AAPH, very-low-density lipoprotein and LDL showed the greatest propensity for hydroperoxide accumulation, whereas HDL seemed relatively resistant. Estimates for plasma and LDL peroxidation based upon techniques which measure total lipid hydroperoxides suggest that levels of hydroperoxides in plasma and LDL are far higher than that those estimates generated by ostensibly more selective techniques. Higher levels of hydroperoxides in LDL than those reported by HPLC-chemiluminescence also seem in greater accordance with other available data concerning LDL oxidation.

Specificity of the ferrous oxidation of xylenol orange assay: analysis of autoxidation products of cholesteryl arachidonate

2003 ◽  
Vol 313 (2) ◽  
pp. 319-326 ◽  
Author(s):  
Huiyong Yin ◽  
Ned A Porter
Keyword(s):  
Xylenol Orange ◽  
Ferrous Oxidation

Application of the ferrous oxidation–xylenol orange assay for the screening of 5-lipoxygenase inhibitors

2006 ◽  
Vol 351 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Young Sik Cho ◽  
Hyo Sun Kim ◽  
Chi Hyun Kim ◽  
Hyae Gyeong Cheon
Keyword(s):  
Xylenol Orange ◽  
Lipoxygenase Inhibitors ◽  
Ferrous Oxidation
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