Differential Expression of Anthocyanin Biosynthetic Genes and Anthocyanin Accumulation in Tartary Buckwheat Cultivars ‘Hokkai T8’ and ‘Hokkai T10’

2011 ◽  
Vol 59 (6) ◽  
pp. 2356-2361 ◽  
Author(s):  
Nam Il Park ◽  
Xiaohua Li ◽  
Tatsuro Suzuki ◽  
Sun-Ju Kim ◽  
Sun-Hee Woo ◽  
...  
PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e19455 ◽  
Author(s):  
Yong-Zan Wei ◽  
Fu-Chu Hu ◽  
Gui-Bing Hu ◽  
Xiao-Jing Li ◽  
Xu-Ming Huang ◽  
...  

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46070 ◽  
Author(s):  
Li Li ◽  
Zhao-Jun Ban ◽  
Xi-Hong Li ◽  
Mao-Yu Wu ◽  
Ai-Li Wang ◽  
...  

2020 ◽  
Vol 10 (4) ◽  
pp. 651-663
Author(s):  
Lei Yu ◽  
Ya Zhou ◽  
Yihui Zhang ◽  
Wei Liu ◽  
Yongqiang Li ◽  
...  

BACKGROUND: DNA methylation balance is an important regulatory mechanism for mammalian and plant development. The fruit ripening and anthocyanin accumulation of Vaccinium corymbosum are complex developmental processes that involve numerous physiological, biochemical, and structural alterations. OBJECTIVE: This study aimed to investigate the correlation of DNA methylation balance, DNA methylation and demethylation-related gene expression models and anthocyanin accumulation during blueberry fruit ripening. METHODS: The anthocyanin contents during V. corymbosum ‘O’Neal’ fruit development were evaluated. The V. corymbosum DNA methylation- and anthocyanin accumulation-related genes were isolated, and their relative expression patterns were detected during flower bud enlargement and fruit development. Moreover, the relative expression patterns of anthocyanin biosynthetic genes and the dynamic changes in the DNA methylation of the promoter sequences of key anthocyanin biosynthetic genes were evaluated. RESULTS: The results showed that the DNA methylation level of V. corymbosum fruit was consistent with anthocyanin accumulation during ripening, and the expression levels of anthocyanin biosynthetic and DNA methylation-related genes. CONCLUSIONS: During V. corymbosum fruit ripening, anthocyanin accumulation is regulated partially by DNA methylation balance of VcCHS and VcANS promoters.


2012 ◽  
Vol 455-456 ◽  
pp. 443-448
Author(s):  
Bo Zhou ◽  
Shu Hua Yan ◽  
Yu Hua Li

Anthocyanins are the main pigments in flowers and fruits. In most cases, anthocyanin accumulation in fruit is highly controlled by the developmental level. In this study, the cDNA fragments of three genes, chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS), which are involved in the flavonoid pathway, were isolated from total RNA of strawberry ripe fruit by using polymerase chain reaction technique and labeled as probes to determine the expression of anthocyanin biosynthetic genes. Northern analysis showed that a correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of strawberry fruits. At the early stages of fruit development, the mRNA levels encoding CHS, DFR, ANS were high probably responsible for the accumulation of condensed tannins, but the levels decreased dramatically when fruits turned white from green. During the stage of pigment accumulation, their mRNA levels increased strongly to be involved anthocyanin biosynthesis. Difference of CHS in mRNA abundance was correlated with differential accumulation of anthocyanins throughout the process of fruit development. Therefore, CHS could be a key structure gene involved in anthocyanin synthesis. Furthermore, the co-ordination of expression of anthocyanin biosynthetic genes implied a common regulatory mechanism controlling the expression of structural genes in the flavonoid pathway.


2011 ◽  
Vol 59 (11) ◽  
pp. 6034-6039 ◽  
Author(s):  
Nam Il Park ◽  
Hui Xu ◽  
Xiaohua Li ◽  
In Hyuk Jang ◽  
Suhyoung Park ◽  
...  

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