Development of a Microsatellite-Based Method for the Differentiation of European Wild Boar (Sus scrofa scrofa) from Domestic Pig Breeds (Sus scrofa domestica) in Food

2012 ◽  
Vol 60 (13) ◽  
pp. 3341-3347 ◽  
Author(s):  
Christine M. Conyers ◽  
Theodore R. Allnutt ◽  
Heather J. Hird ◽  
Joy Kaye ◽  
James Chisholm
2020 ◽  
Vol 9 (32) ◽  
Author(s):  
S. Ndlovu ◽  
A.-L. Williamson ◽  
L. Heath ◽  
O. Carulei

ABSTRACT We report here the genome sequences of three African swine fever virus isolates obtained from a domestic pig (Zaire [Zaire]), a warthog (RSA/W1/1999 [South Africa]), and a European wild boar (RSA/2/2004 [South Africa]) belonging to genotypes IV, XX, and XX, respectively. This report increases the number of genotype XX, wild boar, and warthog reference sequences available.


2016 ◽  
Vol 47 (6) ◽  
pp. 682-690 ◽  
Author(s):  
L. Iacolina ◽  
V. Brajković ◽  
A. Canu ◽  
N. Šprem ◽  
V. Cubric-Curik ◽  
...  

2010 ◽  
Vol 101 (4) ◽  
pp. 797-822 ◽  
Author(s):  
PAULO CÉLIO ALVES ◽  
IRIS PINHEIRO ◽  
RAQUEL GODINHO ◽  
JOAQUIN VICENTE ◽  
CHRISTIAN GORTÁZAR ◽  
...  

2019 ◽  
pp. 21-25
Author(s):  
Bendegúz Mihalik ◽  
Krisztián Frank ◽  
Dániel Szemethy ◽  
Viktor Stéger ◽  
Szilvia Kusza

Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds. The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3. After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds. In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.


Genetics ◽  
2000 ◽  
Vol 154 (4) ◽  
pp. 1785-1791 ◽  
Author(s):  
E Giuffra ◽  
J M H Kijas ◽  
V Amarger ◽  
Ö Carlborg ◽  
J-T Jeon ◽  
...  

Abstract The domestic pig originates from the Eurasian wild boar (Sus scrofa). We have sequenced mitochondrial DNA and nuclear genes from wild and domestic pigs from Asia and Europe. Clear evidence was obtained for domestication to have occurred independently from wild boar subspecies in Europe and Asia. The time since divergence of the ancestral forms was estimated at ~500,000 years, well before domestication ~9,000 years ago. Historical records indicate that Asian pigs were introduced into Europe during the 18th and early 19th centuries. We found molecular evidence for this introgression and the data indicated a hybrid origin of some major “European” pig breeds. The study is an advance in pig genetics and has important implications for the maintenance and utilization of genetic diversity in this livestock species.


2018 ◽  
Vol 98 (3) ◽  
pp. 557-564
Author(s):  
Martyna Batorska ◽  
Justyna Więcek ◽  
Małgorzata Kunowska-Slósarz ◽  
Kamila Puppel ◽  
Jan Slósarz ◽  
...  

The objective of this study was to investigate the effect of carcass weight on chemical characteristics and fatty acid composition of m. longissimus dorsi and m. semimembranosus of European wild boar (Sus scrofa scrofa) meat. The research was carried out on 50 wild boars shot during four battues at hunting reserves in the central part of Poland. The carcasses were divided into three groups: ≤30 kg (G1), 31–45 kg (G2), and >45 kg (G3). The protein concentration increased with carcass weight (P ≤ 0.01). The concentration of saturated fatty acids was influenced by carcass weight and was higher when weight increased. A significantly higher level of monounsaturated fatty acids (MUFA) was found in the carcasses of wild boars from G3 compared with G1 group (P ≤ 0.01). The content of C18:2n-6 was significantly lower in the lightest carcasses compared with the heaviest (P ≤ 0.05). Statistical analysis confirmed that carcasses weight significantly affected polyunsaturated fatty acids (PUFA) n-3 content. The lightest carcasses contained a significantly higher concentration of PUFA n-3 (P ≤ 0.01). In addition, in G3, the amount of C22:5n-3 was two-fold lower (P ≤ 0.01) than in G1. The research has shown that the meat quality was affected by the carcass weight, resulting better parameters in the lighter than in the heavier carcasses. Therefore, most of the culled wild boars should be squeakers and young boars after the first year of life.


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