Inhibition of Penicillium digitatum and Penicillium italicum by Hydroxypropyl Methylcellulose−Lipid Edible Composite Films Containing Food Additives with Antifungal Properties

Aim: Self-healing, swellable and biodegradable polymers are vital materials that may facilitate the different stages of wound healing. The aim of this research was to prepare wound healing films using self-healing polyvinyl alcohol (PVA), swellable hydroxypropyl methylcellulose (HPMC), biodegradable polyglycolic acid (PGA) sutures and ciprofloxacin antibiotic for improved treatment outcome. Methods: Films were formulated through aqueous-based mixing of varying amounts of polyvinyl alcohol (10–20% weight/weight (w/w)) and hydroxypropyl methylcellulose (0.5, 1% w/w) with fixed quantities of ciprofloxacin. PGA sutures were placed as grids within the wet mixtures of the polymers and ciprofloxacin, and thereafter products were air dried. The formulated films were evaluated for swelling ratio, breaking elongation, folding endurance, moisture uptake and loss, compatibility and in vitro antibiotic release. Furthermore, in vivo wound healing was studied using excision model and histopathological examinations. Results: Swelling ratios were above 1.0 and the films were minimally stretchable, with folding endurance greater than 500. Films were stable while moisture uptake and loss were observed to be less than 30%. Among the optimised hydrogel batches, those containing 10% w/w PVA and 1% w/w HPMC with no PGA showed the highest drug release of 73%, whereas the batches with higher PGA content showed higher percentage wound size reduction with minimal scar. The completeness of wound healing with batches containing PVA, HPMC, ciprofloxacin and PGA, along with the standard, is evident considering the massive cornification, regeneration of the epithelial front and stratum spinosum. Conclusion: The findings show that polymer-based multifunctional composite films are suitable for use as dressings for improved wound healing.

Download Full-text

Uso de fungicidas en el manejo postcosecha de limón persa (Citrus Latifolia)

Revista Biológico Agropecuaria Tuxpan ◽

10.47808/revistabioagro.v6i2.202 ◽

2018 ◽

Vol 6 (2) ◽

pp. 107-111

Author(s):

María del Rosario Dávila Lezama ◽

Néstor Manuel Lorenzo Flores ◽

Teresita Ramírez Hernández ◽

María Alva Ángel Lara ◽

Carlos Jesús Real Garrido

Keyword(s):

Botrytis Cinerea ◽

Geotrichum Candidum ◽

Penicillium Digitatum ◽

Rhizopus Stolonifer ◽

Penicillium Italicum ◽

Citrus Latifolia ◽

Penicillium Spp

Estudios realizados, han identificado que los hongos responsables que limitan la vida de anaquel de los cítricos son principalmente: Penicillium digitatum (55-80%); Penicillium italicum (2-30%); Alternaria citri y A. alternata (8-15%); Botrytis cinerea (8-20%): Colletotrichum gloesporioides (2.5-6%); Geotrichum candidum (2-3%); Rhizopus stolonifer y R. oryzae (1-3%); Phytophtora citrophtora (2%) (Salvador et al., 2007). El objetivo del experimento Evaluar la efectividad de dos fingicidas para el control de enfermedades provocadas por hongos en limón persa (Citrus latifolia) en postcosecha. El Proyecto se realizó en Cuajilote, Cuitláhuac, Ver. Trasladando las muestras al laboratorio general número 4 de la Facultad de Ciencias Biológicas y Agropecuarias, región Orizaba-Córdoba, de la Universidad Veracruzana. Los tratamientos donde se aplicaron los fungicidas Bankit Gold® (Azoxystrobin + Fludioxonil) y Magnate Sulphate® (Imazalil) en limón persa (Citrus latifolia) en el proceso de postcosecha, no tuvieron presencia de patógenos que provocan daños en el fruto por lo cual los fungicidas cumplieron con su objetivo, sin embargo, el tratamiento 1 (testigo absoluto) tuvo presencia del patógeno Penicillium spp. en su evaluación a los 30 DDA, esto, basándonos en los resultados de los análisis microbiológicos de limón persa (Citrus latifolia), la contaminación por Penicillium spp. probablemente fue en el almacenamiento del limón persa (Citrus latifolia). Respecto a los resultados de las propiedades fisicoquímicas están dentro los parámetros de calidad.

Download Full-text

Antimicrobial soy protein isolate-based films: physical characterisation, active agent retention and antifungal properties against Penicillium italicum

International Journal of Food Science & Technology ◽

10.1111/ijfs.13664 ◽

2017 ◽

Vol 53 (4) ◽

pp. 921-929 ◽

Cited By ~ 6

Author(s):

Ramsés R. González-Estrada ◽

Montserrat Calderón-Santoyo ◽

Juan A. Ragazzo-Sánchez ◽

Stephane Peyron ◽

Pascale Chalier

Keyword(s):

Soy Protein ◽

Soy Protein Isolate ◽

Active Agent ◽

Protein Isolate ◽

Penicillium Italicum ◽

Antifungal Properties

Download Full-text

Decay Detection in Citrus Fruits Using Hyperspectral Computer Vision

Intelligent Data Analysis for Real-Life Applications ◽

10.4018/978-1-4666-1806-0.ch006 ◽

2012 ◽

pp. 104-123

Author(s):

Juan Gómez-Sanchis ◽

Emilio Soria-Olivas ◽

Delia Lorente-Garrido ◽

José M. Martínez-Martínez ◽

Pablo Escandell-Montero ◽

...

Keyword(s):

Computer Vision ◽

Agricultural Sector ◽

Vision System ◽

Penicillium Digitatum ◽

Machine Learning Techniques ◽

Economic Losses ◽

Citrus Fruits ◽

Penicillium Italicum ◽

Citrus Industry ◽

Learning Techniques

The citrus industry is nowadays an important part of the Spanish agricultural sector. One of the main problems present in the citrus industry is decay caused by Penicillium digitatum and Penicillium italicum fungi. Early detection of decay produced by fungi in citrus is especially important for the citrus industry of distribution. This chapter presents a hyperspectral computer vision system and a set of machine learning techniques in order to detect decay caused by Penicillium digitatum and Penicillium italicum fungi that produce more economic losses to the sector. More specifically, the authors employ a hyperspectral system and artificial neural networks. Nowadays, inspection and removal of damaged citrus is done manually by workers using dangerous ultraviolet light. The proposed system constitutes a feasible and implementable solution for the citrus industry; this has been proven by the fact that several machinery enterprises have shown their interest in the implementation and patent of the system.

Download Full-text

Non-chemical treatments for preventing the postharvest fungal rotting of citrus caused by Penicillium digitatum (green mold) and Penicillium italicum (blue mold)

Trends in Food Science & Technology ◽

10.1016/j.tifs.2019.02.053 ◽

2019 ◽

Vol 86 ◽

pp. 479-491 ◽

Cited By ~ 34

Author(s):

Konstantinos Papoutsis ◽

Matthaios M. Mathioudakis ◽

Joaquín H. Hasperué ◽

Vasileios Ziogas

Keyword(s):

Penicillium Digitatum ◽

Penicillium Italicum ◽

Blue Mold ◽

Chemical Treatments ◽

Green Mold

Download Full-text

Individual and Combined Effects of Vanillin and Potassium Sorbate on Penicillium digitatum, Penicillium glabrum, and Penicillium italicum Growth

Journal of Food Protection ◽

10.4315/0362-028x-62.5.541 ◽

1999 ◽

Vol 62 (5) ◽

pp. 541-542 ◽

Cited By ~ 24

Author(s):

B. MATAMOROS-LEÓN ◽

A. ARGAIZ ◽

A. LÓPEZ-MALO

Keyword(s):

Water Activity ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Potato Dextrose Agar ◽

Penicillium Digitatum ◽

Potassium Sorbate ◽

Combined Effects ◽

Fractional Inhibitory Concentration ◽

Penicillium Italicum ◽

The Individual

The individual and combined effects of potassium sorbate and vanillin concentrations on the growth of Penicillium digitatum, P. glabrum, and P. italicum in potato dextrose agar adjusted to water activity 0.98 and pH 3.5 were evaluated. Inhibitory concentrations of potassium sorbate varied from 150 ppm for P. digitatum to 700 ppm for P. glabrum, and for vanillin from 1,100 ppm for P. digitatum and P. italicum and 1,300 ppm for P. glabrum. Fractional inhibitory concentration (FIC) isobolograms show curves deviated to the left of the additive line. Calculated FIC index varied from 0.60 to 0.84. FIC index as well as FIC isobolograms show synergistic effects on mold inhibition when vanillin and potassium sorbate are applied in combination.

Download Full-text