A Model Macroion Solution Next to a Charged Wall:  Overcharging, Charge Reversal, and Charge Inversion by Macroions

2004 ◽  
Vol 108 (22) ◽  
pp. 7286-7296 ◽  
Author(s):  
Felipe Jiménez-Ángeles ◽  
Marcelo Lozada-Cassou
Keyword(s):  
Charge Inversion ◽  
Charge Reversal

scholarly journals The role of pH, metal ions and their hydroxides in charge reversal of protein-coated nanoparticles

2019 ◽  
Vol 21 (21) ◽  
pp. 11011-11018 ◽  
Author(s):  
Jonas Schubert ◽  
Carmen Radeke ◽  
Andreas Fery ◽  
Munish Chanana
Keyword(s):  
Metal Ions ◽  
Au Nanoparticles ◽  
Charge Inversion ◽  
Charge Reversal ◽  
Metal Hydroxides ◽  
Coated Nanoparticles

In this study, we investigated charge inversion of protein-coated Au nanoparticles caused by the addition of metal ions. Adsorbed metal hydroxides were identified to cause the charge inversion of the NPs by using a combination of cryo-TEM, EFTEM andζ-potential measurements.

Direct Measurement of Charge Reversal on Lipid Bilayers using Heterodyne-Detected Second Harmonic Generation Spectroscopy

2019 ◽  
Author(s):  
HanByul Chang ◽  
Paul Ohno ◽  
Yangdongling Liu ◽  
Franz Geiger
Keyword(s):  
Lipid Bilayers ◽  
Surface Potential ◽  
Second Harmonic ◽  
Fluid Phase ◽  
Cationic Polyelectrolyte ◽  
Charge Reversal ◽  
Buried Interfaces ◽  
Phase Transition Temperatures ◽  
Complementary Techniques ◽  
Order Susceptibility

We report the detection of charge reversal induced by the adsorption of a cationic polyelectrolyte, poly(allylamine) hydrochloride (PAH), to buried supported lipid bilayers (SLBs), used as idealized model biological membranes. We observe changes in the surface potential in isolation from other contributors to the total SHG response by extracting the phase-shifted potential-dependent third-order susceptibility from the overall SHG signal. We demonstrate the utility of this technique in detecting both the sign of the surface potential and the point of charge reversal at buried interfaces without any prior information or complementary techniques<i>.</i>Furthermore, isolation of the second-order susceptibility contribution from the overall SHG response allows us to directly monitor changes in the Stern Layer. Finally, we characterize the Stern and Diffuse Layers over single-component SLBs formed from three different zwitterionic lipids of different gel-to-fluid phase transition temperatures (T<sub>m</sub>s). We determine whether the surface potential changes with the physical phase state (gel, transitioning, or fluid) of the SLB and incorporate 20 percent of negatively charged lipids to the zwitterionic SLB to investigate how the surface potential changes with surface charge.

Synergistic Charge Reversal with Hierarchical Architecture for Self-Adaptive Antibacterial Surface of Polymer Substrate

2020 ◽  
Author(s):  
Tingwu Liu ◽  
Shunjie Yan ◽  
Rongtao Zhou ◽  
Xu Zhang ◽  
Huawei Yang ◽  
...  
Keyword(s):  
Polymer Substrate ◽  
Hierarchical Architecture ◽  
Charge Reversal ◽  
Antibacterial Surface ◽  
Self Adaptive

Evidence of charge inversion in the reaction of singly charged anions with multiply charged macroions

1991 ◽  
Vol 95 (17) ◽  
pp. 6412-6415 ◽  
Author(s):  
Rachel R. Ogorzalek Loo ◽  
Harold R. Udseth ◽  
Richard D. Smith
Keyword(s):  
Charge Inversion ◽  
Multiply Charged ◽  
Singly Charged

scholarly journals pH and redox dual-sensitive drug delivery system constructed based on fluorescent carbon dots

RSC Advances ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 2656-2663
Author(s):  
Boye Zhang ◽  
Qianqian Duan ◽  
Yi Li ◽  
Jianming Wang ◽  
Wendong Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Drug Delivery System ◽  
Delivery System ◽  
Carbon Dots ◽  
Ph Responsive ◽  
Charge Reversal ◽  
Fluorescent Carbon Dots

The system is pH-responsive and redox-controlled release. And the charge reversal and size transitions of the system can enhance the targeted ability. Moreover, the system can recognize the cancer cells by the fluorescence imaging.

scholarly journals NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Alina Spielmann ◽  
Yannik Brack ◽  
Hugo van Beek ◽  
Lion Flachbart ◽  
Lea Sundermeyer ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Catalytic Properties ◽  
Single Charge ◽  
Protein Surface ◽  
Charge Reversal

scholarly journals Tumor acidity-induced charge-reversal liposomal doxorubicin with enhanced cancer cell uptake and anticancer activity

Giant ◽  
2021 ◽  
Vol 6 ◽  
pp. 100052 ◽  
Author(s):  
Jinpeng Yang ◽  
Zihe Yin ◽  
Yincheng Chang ◽  
Hua Wang ◽  
Jiang-Fei Xu ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cancer Cell ◽  
Liposomal Doxorubicin ◽  
Cell Uptake ◽  
Charge Reversal ◽  
Tumor Acidity ◽  
Induced Charge

Charge reversal in real colloids: Experiments, theory and simulations

2008 ◽  
Vol 319 (1-3) ◽  
pp. 103-108 ◽  
Author(s):  
Alberto Martín-Molina ◽  
José Alberto Maroto-Centeno ◽  
Roque Hidalgo-Álvarez ◽  
Manuel Quesada-Pérez
Keyword(s):  
Charge Reversal

Reconstitution of human atlastin fusion activity reveals autoinhibition by the C terminus

2021 ◽  
Vol 221 (2) ◽  
Author(s):  
Daniel Crosby ◽  
Melissa R. Mikolaj ◽  
Sarah B. Nyenhuis ◽  
Samantha Bryce ◽  
Jenny E. Hinshaw ◽  
...  
Keyword(s):  
Network Formation ◽  
Fusion Activity ◽  
Amphipathic Helix ◽  
Alternate Splicing ◽  
Charge Reversal ◽  
Splice Isoforms ◽  
C Terminus ◽  
Splice Isoform ◽  
Inhibitory Domain ◽  
Α Helix

ER network formation depends on membrane fusion by the atlastin (ATL) GTPase. In humans, three paralogs are differentially expressed with divergent N- and C-terminal extensions, but their respective roles remain unknown. This is partly because, unlike Drosophila ATL, the fusion activity of human ATLs has not been reconstituted. Here, we report successful reconstitution of fusion activity by the human ATLs. Unexpectedly, the major splice isoforms of ATL1 and ATL2 are each autoinhibited, albeit to differing degrees. For the more strongly inhibited ATL2, autoinhibition mapped to a C-terminal α-helix is predicted to be continuous with an amphipathic helix required for fusion. Charge reversal of residues in the inhibitory domain strongly activated its fusion activity, and overexpression of this disinhibited version caused ER collapse. Neurons express an ATL2 splice isoform whose sequence differs in the inhibitory domain, and this form showed full fusion activity. These findings reveal autoinhibition and alternate splicing as regulators of atlastin-mediated ER fusion.

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